污染控制技术及原理
刘洁生,陈芝兰,杨维东,陈伟峰.凤眼莲根系丙酮提取物抑制赤潮藻类生长的机制研究[J].环境科学学报,2006,26(5):815-820
凤眼莲根系丙酮提取物抑制赤潮藻类生长的机制研究
- Inhibitory mechanism of acetone extract from Eichhornia crassipes root on Alexandrium tamarense
- 基金项目：国家重点基础研究计划(973)项目(No.2001CB409710);广东省科技计划项目(No.2004B20501007)
- 作者
- 单位
- 刘洁生
- 暨南大学生物工程学系, 广州 510632
- 陈芝兰
- 暨南大学生物工程学系, 广州 510632
- 杨维东
- 暨南大学生物工程学系, 广州 510632
- 陈伟峰
- 暨南大学生物工程学系, 广州 510632
- 摘要：为了探讨凤眼莲根系丙酮提取物抑制塔玛亚历山大藻(Alexandrium tamarense)生长的机制,筛选和发现新的、高效、低毒或无毒、能迅速控制赤潮生物生长的除藻剂.对比分析了凤眼莲根系丙酮提取物中不同物质的抑藻效果,探讨了凤眼莲根系丙酮提取物抑藻的主要化学基础通过考查主要抑藻物质对藻细胞可溶性蛋白质、丙二醛含量和超氧化物歧化酶(SOD)活力的影响,探讨其抑藻机理.结果表明,浓度高于5m·L-1以上的N—苯基—2-萘胺,3d后能保持对塔玛亚历山大藻抑制率超过50%;浓度为70μL·L-1的亚油酸对塔玛亚历山大藻的抑制率约为40%;浓度为70μL·L-1的壬酸第3天可以达到85%的抑藻率,但随后藻密度有反弹.实验浓度范围内的亚油酸甘油酯和丙酰胺对塔玛亚历山大藻的抑制作用不明显.培养液中加入一定量N—苯基—2-萘胺后,藻体中的可溶性蛋白质和丙二醛含量有应激性升高,超氧化物歧化酶活力呈下降趋势.研究结果表明,N—苯基—2-萘胺可能是凤眼莲根系丙酮提取物中的主要抑藻物质;其可能通过自由基反应破坏藻细胞的结构,从而达到抑藻效果.
- Abstract：The chemical basis and mechanism of the antialgal effect of acetone extract from Eichhornia crassipes roots were discussed to screen newly and efficient algaecides in controlling HAbs. The inhibitory activities of chemicals in the acetone extract from Eichhornia crassipes root were determinated. On the basis of comparison in removal efficiency and toxicity of the chemicals assayed, the effect of N-phenyl-2-naphthylamine, the best candidate to control HAbs, on protein, MDA and SOD of A. tamarense was studied. 5 mg·L-1 of N-phenyl-2-naphthylamine was showed to have 50% inhibitory rate on A. tamarense after 3 days. 70 μL·L-1 of linoleic acid appeared 40% inhibition rate on A. tamarense.70 μL·L-1 of pelargonic acid had 85% inhibition rate at the 3 rd day with some increase in cell density later on. However, glyceryl linoleate and propionamide in the experimental concentrations had less inhibition on A. tamarense. N-phenyl-2-naphthylamine induced some increases in protein and MDA, but led to decrease in SOD activity in A. tamarense. These suggested that N-phenyl-2-naphthylamine might play an important role in the inhibition, and that allelochemicals in E. crassipes might damage the algae cell structure through free radical reaction. The allelochemicals from E.crassipes roots such as N-phenyl-2-naphthylamine might be a potential candidate in HABs control.
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