• 关键词：聚丙烯酰胺  原油  降解  假单胞菌PD-1

• 基金项目："九五"中国石油天然气集团公司攻关项目(961210609)

• 李蔚
• 南开大学生命科学院,天津300071

• 刘如林
• 南开大学生命科学院,天津300071

• 梁凤来
• 南开大学生命科学院,天津300071

• 杨振宇
• 大庆油田勘探开发研究院,大庆163712

• 石梅
• 大庆油田勘探开发研究院,大庆163712

• 摘要：以聚丙烯酰胺为能源和碳源从油田采出水中分离到一株菌, 根据其生理生化特性分析及生长试验, 该株菌被鉴定为假单胞菌属PD-1菌株。 对该菌的性能进行了评价, 证明该菌能够在含原油、聚丙烯酰胺的水环境中生长, 并对原油和聚丙烯酰胺具有降解作用。 化学分析表明, 在细菌的降解下, 原油物性发生改变, 姥鲛烷/nC₁₇和植烷/nC₁₈以及∑C₂₁^-∑C₂₂⁺和(C₂₁

• Abstract：By the isolation and incubation technology, a microbial strain that could grow with crude oil and polyacrylamide had been screened. According to its physiological and biochemical characteristics, the selected bacterium was identified as a strain belonging to Pseudomonas sp. Genus, named PD-1. The evaluation showed that the crude oil and polyacrylamide could be biodegraded by the bacteria and the physical characteristics of the crude oil had been changed, resulting in the increase of Pr/nC₁₇,Ph/nC₁₈,∑C^-₂₁/∑C⁺₂₂and (C₂₁+C₂₂)/(C₂₈+C₂₉).The crude oil was degraded into hexadecanic acid, propanoic acid and butenic acid. At the same time, the structure of polyacrylamide was destroyed, resulting in the reduction of the viscosity and the molecular weight.

• Key words：polyacrylamide  crude oil  degradation  Pseudomonas sp  PD-1

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