研究报告
刘光涛,周长芳,孙利芳,朱薇薇,姜昊,王涵兴,安树青.凤眼莲化感物质对铜绿微囊藻、斜生栅藻生长及细胞数相对比例的影响[J].环境科学学报,2011,31(10):2303-2311
凤眼莲化感物质对铜绿微囊藻、斜生栅藻生长及细胞数相对比例的影响
- Effects of Eichhornia crassipes allelochemicals on the growth of two mono- and co-cultured algae Microcystis aeruginosa and Scenedesmus obliquus
- 基金项目：国家自然科学基金(No. 30970458, 30770358); 国家重点基础研究发展计划(973)项目(No.2008CB418004)
- 作者
- 单位
- 刘光涛
- 南京大学生命科学院,南京 210093
- 周长芳
- 南京大学生命科学院,南京 210093
- 孙利芳
- 南京大学生命科学院,南京 210093
- 朱薇薇
- 南京大学生命科学院,南京 210093
- 姜昊
- 南京大学生命科学院,南京 210093
- 王涵兴
- 南京大学生命科学院,南京 210093
- 安树青
- 南京大学生命科学院,南京 210093
- 摘要：为探索化感物质在蓝藻水华治理方面的应用潜力,选取在凤眼莲根系分泌物中发现的5种化感物质(亚油酸、亚油酸甘油酯、丙酰胺、N-苯基—2-萘胺和壬酸),研究了3种剂量下(0.1、1.0、10.0 mg·L-1)其对单一、混合培养铜绿微囊藻和斜生栅藻生长的影响.结果发现,化感物质成分、剂量和藻类培养方式对铜绿微囊藻和斜生栅藻生长都具有极显著的影响(p<0.001),化感物质剂量相对而言影响力最大.5种化感物质对两种藻类的生长总体上呈低剂量促进高剂量抑制和先促进后抑制的趋势.其中,壬酸对两种藻的作用均极强,另外,丙酰胺对铜绿微囊藻、亚油酸对斜生栅藻的作用也较强.0.1、1.0 mg·L-1的壬酸和10 mg·L-1的亚油酸、亚油酸甘油酯、丙酰胺、N-苯基—2-萘胺都显著增加了混合培养物中斜生栅藻的相对比例(p<0.05).本研究表明上述凤眼莲化感物质在蓝藻水华治理中具有良好的应用前景.
- Abstract：To explore the possibility of using allelochemicals in solving the problem of algal blooms in eutrophicated shallow lakes, five allelochemicals found from roots of Eichhornia crassipes (linoleic acid, 1-linoleoyl-rac-glycerol, propionamide, N-phenyl-2-naphthylamine and nonanoic acid) were tested with mono- and co-cultured algae Microcystis aeruginosa and Scenedesmus obliquus. The results showed that components and doses of the allelochemicals, as well as culture modes, affected the growth of the two algae significantly (p<0.001). The allelochemicals generally promoted the growth of the algae when added at low concentrations (0.1~1.0 mg·L-1) and during early culture stages, while such effects turned into inhibition when allelochemical concentration was increased (10.0 mg·L-1) and the culture stage was extended. Among the fived allelochemicals tested, nonanoic acid had the strongest effect on both algae, whereas individually, M. aeruginosa was most affected by propionamide, and S. obliquus by linoleic acid. Cell number proportions of S. obliquus in co-cultures were also increased by nonanoic acid at a concentration of 0.1~1.0 mg·L-1 and the other allelochemicals at 10.0 mg·L-1 (p<0.05). This research indicated a promising potential of the application of Eichhornia crassipes allelochemicals in control of aquatic algae.
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