• 关键词：餐厨垃圾  厌氧消化  PCR-TGGE  微生物群落结构

• 基金项目：国家"十一五"科技支撑计划项目(No.2010BAC67B01);重庆市科技计划重点项目

• 彭绪亚
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 邸玉翠
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 贾传兴
• 1. 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045;
  2. 济宁市环境保护局,济宁 272000

• 梅冰
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 王璐
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 洪俊华
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 李小风
• 重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

• 摘要：为了解不同负荷下单相餐厨垃圾厌氧消化反应器内微生物群落结构演替特征,在单相厌氧消化反应器负荷为2.0~8.5 kg·m^-3·d^-1(以VS计)的不同负荷条件下取样,运用16S rDNA的PCR-TGGE技术对反应器内微生物进行动态追踪.同时,运用Dice系统和NMDS软件对PCR-TGGE图谱进行分析.结果表明,负荷为4.0~6.0 kg·m^-3·d^-1时,微生物群落结构变化不大;负荷为6.0~7.0 kg·m^-3·d^-1时,微生物群落结构变化较为明显;负荷分别为7.0~8.0 kg·m^-3·d^-1及8.5 kg·m^-3·d^-1时,微生物群落结构变化最为明显.纵观整个过程,在餐厨垃圾厌氧消化反应器有机负荷在2.0~8.5 kg·m^-3·d^-1下厌氧反应器内的微生物群落结构存在明显的阶段性演替;负荷为7.0 kg·m^-3·d^-1时微生物群落结构的丰富度最好.

• Abstract：The objective of this study was to better understand the transition of microbial communities during the anaerobic digestion (AD) of food waste at different volumetric organic loading rates. Tests were conducted in laboratory scale, single-phase reactors operated at loading rates ranged from 2.0 to 8.5 kg·m^-3·d^-1. The microbial community structure and dynamics were characterized by the 16S rDNA-based polymerase chain reaction-temperature gradient gel electrophoresis (PCR-TGGE) assay. The TGGE profiles analyzed by Dice similarity coefficients method and non-metric multidimensional scaling (NMDS) method indicated that the microbial community structure varied significantly at loading rates of 7.0~8.0 kg·m^-3·d^-1 and 8.5 kg·m^-3·d^-1, respectively, but insignificantly at the loading rate of 4.0~6.0 kg·m^-3·d^-1. In summary, significant phase transition in the microbial community structure occurred at the full range of 2.0 to 8.5 kg·m^-3·d^-1, with an optimal diversity of microbial communities observed at 7.0 kg·m^-3·d^-1.

• Key words：food waste  anaerobic digestion  PCR-TGGE  microbial community structure

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  摘要点击次数： 2015 全文下载次数： 3467