• 关键词：黄松田土壤  外源甲烷  甲烷氧化活性  碳素  氮素

• 基金项目：国家自然科学基金(39870033);中国科学院南京土壤研究所开放实验室(971531);国家教育部留学回国人员基金项目

• 闵航
• 浙江大学生命科学学院生物科学系, 杭州 310029

• 陈中云
• 浙江大学生命科学学院生物科学系, 杭州 310029

• 吴伟祥
• 浙江大学生命科学学院生物科学系, 杭州 310029

• 陈美慈
• 浙江大学生命科学学院生物科学系, 杭州 310029

• 摘要：研究了碳素和氮素物质对黄松田土壤中甲烷氧化菌种群及其甲烷氧化活性的影响.结果表明,加入不同碳素或氮素物质对黄松田土中甲烷氧化菌种群的数量变化无显著性影响,但对土壤氧化外源甲烷活性却具有显著性影响.且不同浓度的同一物质对黄松田土壤氧化外源甲烷速率的影响也不相同.甲烷氧化代谢途径中的中间产物(甲醇和甲酸)较非甲烷氧化代谢途径中的中间产物和能促进土壤产甲烷菌产甲烷活性的碳源物质(酵母膏、葡萄糖和乙酸)可更强烈地抑制土壤氧化外源甲烷的活性.有机氮素物质对黄松田土的甲烷氧化速率的影响要较无机氮素物质弱.在无机氮素物质中,硝酸盐对土壤氧化外源甲烷活性的抑制强度要强于铵盐类物质,NH³要比NH⁴⁺对甲烷氧化菌的甲烷氧化活性具有更强的抑制作用.一旦黄松田土的甲烷氧化活性受到碳、氮物质的抑制,恢复其氧化外源甲烷活性就需要较长时间.且受到抑制的程度越强,恢复所需时间也越长.

• Abstract：The effects of carbon and nitrogen sources on the added methane oxidation and the population methaneoxidizing bacteria in marinefluvigenic yellow loamy paddy soil were studied. The results showed that the various carbon and nitrogen sources affect the added methane oxidizing activity significantly, but not the population of methaneoxidizing bacteria. Also same substrate with different concentrations give various effects on the added methaneoxidizing rate. Formate and methanol, (metabolic intermediates) and yeast extract, glucose and acetate, (nonmetabolic intermediate) are the inhibitors for the added methane oxidation. Inorganic nitrogen The effect on the added methane oxidation rate of inorgamc nitrogen resouce is strongen than organic nitrogen resources. Nitrate is the strongest one. The inhibition of ammonia was stronger to methane oxidation than that of ammonium. There is need a longer time for recovery after the activity of added methane oxidation is inhibited by carbon or nitrogen sources.

• Key words：marinefluvigenic yellow loamy paddy soil  added methane  methaneoxidizing activity  carbon resource  nitrogen source

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