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天津市城郊不同土地利用类型土壤中抗生素分布特征及影响因素分析

裴浩鹏,  徐艳,  陈蕊,  涂棋,  李厚禹,  师荣光

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文章导航 > 环境工程  > 2021 >  39(1): 166-173
裴浩鹏, 徐艳, 陈蕊, 涂棋, 李厚禹, 师荣光. 天津市城郊不同土地利用类型土壤中抗生素分布特征及影响因素分析[J]. 环境工程, 2021, 39(1): 166-173. doi: 10.13205/j.hjgc.202101026
引用本文: 裴浩鹏, 徐艳, 陈蕊, 涂棋, 李厚禹, 师荣光. 天津市城郊不同土地利用类型土壤中抗生素分布特征及影响因素分析[J]. 环境工程, 2021, 39(1): 166-173. doi: 10.13205/j.hjgc.202101026
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PEI Hao-peng, XU Yan, CHEN Rui, TU Qi, LI Hou-yu, SHI Rong-guang. DISTRIBUTION CHARACTERISTICS AND INFLUENCING FACTORS OF ANTIBIOTICS IN SOILS OF DIFFERENT LAND USE TYPES IN SUBURBS OF TIANJIN[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 166-173. doi: 10.13205/j.hjgc.202101026
Citation: PEI Hao-peng, XU Yan, CHEN Rui, TU Qi, LI Hou-yu, SHI Rong-guang. DISTRIBUTION CHARACTERISTICS AND INFLUENCING FACTORS OF ANTIBIOTICS IN SOILS OF DIFFERENT LAND USE TYPES IN SUBURBS OF TIANJIN[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 166-173. doi: 10.13205/j.hjgc.202101026
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天津市城郊不同土地利用类型土壤中抗生素分布特征及影响因素分析

doi: 10.13205/j.hjgc.202101026

  • 1. 沈阳农业大学 土地与环境学院, 沈阳 110866;

  • 2. 农业农村部环境保护科研监测所, 天津 300191;

  • 3. 北京交通大学 土木建筑工程学院, 北京 100091

基金项目: 

中央级公益性科研院所基本科研业务费专项（2019-jbkyywf-xy）；国家自然科学基金青年科学基金项目（41807369）。

详细信息
    作者简介:

    裴浩鹏(1994-),男,硕士,主要研究方向为土壤抗生素的分布特征。1042299572@qq.com

    通讯作者:

    徐艳(1988-),女,博士,助理研究员,主要研究方向为环境有机污染物污染特征研究。xuyan_wa@163.com

    师荣光(1980-),男,博士,研究员,主要研究方向为环境信息技术、产地污染监测与预警、产地安全管理。winsomesky@163.com

DISTRIBUTION CHARACTERISTICS AND INFLUENCING FACTORS OF ANTIBIOTICS IN SOILS OF DIFFERENT LAND USE TYPES IN SUBURBS OF TIANJIN

  • 1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;

  • 2. Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China;

  • 3. School of Civil Eningeering, Beijing Jiaotong University, Beijing 100091, China

    摘要
  • 摘要: 选取了天津市城郊8个不同土地利用类型土壤（包括旱地、公园、林地、滩涂、水浇地、绿化带、居民区、荒地），共48个样本点，对天津不同土地利用类型土壤中抗生素含量、组成、分布特征及其影响因素进行研究。采用超高效液相色谱-质谱联用法（HPLC-MS/MS）检测了5类12种抗生素的残留水平，同时结合其土壤理化性质及微生物群落结构解析影响抗生素污染特征的关键因素。结果表明：抗生素总体检出浓度在（4.35~1.35）×103 μg/kg，总体浓度顺序四环素类（TCs）>磺胺类（SAs）>喹诺酮类（QNs）>大环内酯类（MLs）>β-内酰胺类（β-lactams），其中旱地土壤中抗生素含量显著高于其他土地利用类型土壤抗生素，主要与有机肥施用有关。不同土壤抗生素主成分分析（PCA）发现，旱地和公园土壤中抗生素的组成与其他土壤类型存在较为明显的差异，这与畜禽粪便施肥以及外源生活中抗生素的排放有关；环境因子与抗生素的冗余分析（RDA）结果显示：土壤环境因子[总氮（TN）、总磷（TP）、铜（Cu）、锌（Zn）、pH]对抗生素的组成有较强影响（P<0.05）；微生物与抗生素的斯皮尔曼（Spearman）相关分析发现，浮霉菌属（Planctomyces）、酸性细菌_细菌_WX27（Acidobacteria_bacterium_WX27）、鞘脂单胞菌属（Sphingomonas）、芽孢杆菌属（Gemmatimona）、嗜热光合细菌（Roseiflexus）、类固醇杆菌（Steroidobacter）、溶杆菌属（Lysobacter）、硫化细菌（Thiobacillus）与抗生素相关性达到显著水平（P<0.05），其中微生物与四环素和喹诺酮类抗生素相关性较强。研究结果可为后续土壤抗生素的污染防治工作提供参考。
    Abstract: In this study, a total of 48 sample sites were selected from eight soils with different land use types (including dry land, park, woodland, beach, irrigated land, green belt, residential area and wasteland) in suburban areas of Tianjin, to study the content, composition, distribution characteristics and influencing factors of antibiotics in the soil in Tianjin. Ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was used to detect the residual levels of 12 antibiotics in five categories. Meanwhile, the key factors affecting the pollution characteristics of antibiotics were analyzed by the physical and chemical properties of the soil and microbial community structure. The results showed that the concentration of antibiotics was detected in the range of (4.35~1.35)×103 μg/kg, in the general sequence of tetracyclines (TCs)>sulfonamides (SAs)>quinolones (QNs)>macrolides (MLs)>β-lactams, in which antibiotic content in dry land soil was significantly higher than the other soil, mainly related to organic fertilizer application. Principal component analysis (PCA) showed that the composition of antibiotics in the soil of the dry land and park was significantly different from the others, which was related to fertilization of livestock and poultry manure and the release of antibiotics in exogenous life. Redundant analysis (RDA) of environmental factors and antibiotics showed that soil environmental factors (total nitrogen (TN), total phosphorus (TP), copper (Cu), zinc (Zn), pH) had a strong influence on the composition of antibiotics (P<0.05). Spearman correlation between microorganisms and antibiotics showed that Planctomyce, Sphingomonas, Gemmatimona, Roseiflexus, Steroidobacter, Acidobacteria_bacterium_WX27, Lysobacter, Thiobacillus had significant correlations with antibiotics (P<0.05). Among them, microorganisms were strongly related to tetracycline and quinolone antibiotics. This study could provide a scientific basis for the prevention and control of soil antibiotic pollution.
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