Abstract:Responses of morphological and physiological characteristics in rice (Oryza sativa L.) seedling roots to its uptake of nano-CuO (10, 100mg/L) were investigated using hydroponic experiments by setting micron oxide copper (micron-CuO) and copper ions (Cu2+) as control. Results showed that total copper contents in roots and shoots (roots 351~1444mg/kg dw; shoots 351~1444mg/kg dw) of rice seedlings in nano-CuO treatments (10, 100mg/L) were higher than those in micron-CuO treatments (roots 248~817mg/kg dw; shoots 1.57~1.60mg/kg dw) and Cu2+ treatments (roots147~220mg/kg dw; shoots 14~26mg/kg dw), and furthermore, nano-CuO was observed in TEM images of both root and stem cells, indicating rice seedling can uptake and transfer nano-CuO by the form of nano-CuO. A large amount of nano-CuOs observed in endosomes of rice seedling cells indicated that nano-CuO mainly relied on endocytic pathways to enter cell. The nano-CuO had toxic effects on rice seedling roots and the inhibitory rates on roots morphologies of nano-CuO ranged from 28% to 74%, and root length, root volume and root specific surface area were most sensitive indicators for nano-CuO toxicity. Both nano-CuO bioaccumulation in rice and nanoeffects resulted in toxicities of nano-CuO to rice seedling roots. Under low concentration of nano-CuO strees (10mg/L), root activities of rice seedlings significant improved but no significant differences were observed in its total roots absorbing area and actively absorbing area, compared with the control. Under high concentration of nano-CuO strees (100mg/L), root activities and total roots absorbing area of rice seedlings significantly decreased, compared with control.
刘涛, 向垒, 余忠雄, 莫测辉, 李彦文, 赵海明, 蔡全英, 李慧. 水稻幼苗对纳米氧化铜的吸收及根系形态生理特征响应[J]. 中国环境科学, 2015, 35(5): 1480-1486.
LIU Tao, XIANG Lei, YU Zhong-Xiong, MO Ce-Hui, LI Yan-Wen, ZHAO Hai-Ming, CAI Quan-Ying, LI Hui. Responses of morphological and physiological characteristics in rice (Oryza sativa L.) seedling roots to its uptake of CuO nanopaticles. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(5): 1480-1486.