Effects of high sludge loss on the nitrogen removal performance and microbial community of anammox process
ZHAO Yi-chun1,2,3, LIU Ya-lei2,3, LI Yi-shu2,3, FANG Yuan-yuan2,3, WANG Bo-han2, LI Jia-rui2, WU Dong2, CHANG Ben-ze2, YANG Guang-feng2,3
1. National Engineering Research Center of Marine Facility Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China; 2. Department of Environmental Science and Engineering, Zhejiang Ocean University, Zhoushan 316022, China; 3. Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control, Zhoushan 316022, China
Abstract：In order to study the effects of strong sludge loss on the operation performance, characteristics of granular sludge and nitrogen removal bacteria and functional genes in anaerobic ammonia oxidation (anammox) reactor, a upflow anaerobic sludge blanket (UASB) system was constructed and operated to treat synthetic wastewater. Experimental results showed that the nitrogen removal performance of anammox process was not obviously impaired by high sludge loss, and the total nitrogen (TN) removal efficiency (RE) reached 89.18% after 4days’ recovery. A higher EPS content and a lower PN/PS value of 0.12were beneficial to the formation and agglomeration of anammox granular sludge. The anammox granular sludge with sludge size of > 2mm accounted for 44.9% of the total sludge in this system, and corresponding value was 84.3% for the sludge with size of > 0.5mm, which was advantage to the sludge retention in the reactor. The dominant anammox phyla were Proteobacteria (28.03%), Planctomycetes (15.57%) and Chloroflexi (8.63%), and the domimant genera were anammox bacteria Candidatus Jettenia (9.63%) and Candidatus Brocadia (3.54%). The functional genes involved in anammox reaction included nirS (1.27%), hzs (1.28%), hzo (1.29%), hao (7.04%) and hdh (0.81%), but the presence of denitrification bacteria and related functional genes decreased the stoichiometric ratios Rs (ΔNO2--N/ΔNH4+-N) and Rp (ΔNO3--N/ΔNH4+-N) of anammox reaction.
赵一淳, 刘亚雷, 李亦舒, 方媛媛, 王博涵, 李佳瑞, 吴懂, 畅犇泽, 阳广凤. 强污泥流失对anammox工艺脱氮性能及菌群的影响[J]. 中国环境科学, 2022, 42(5): 2161-2168.
ZHAO Yi-chun, LIU Ya-lei, LI Yi-shu, FANG Yuan-yuan, WANG Bo-han, LI Jia-rui, WU Dong, CHANG Ben-ze, YANG Guang-feng. Effects of high sludge loss on the nitrogen removal performance and microbial community of anammox process. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2161-2168.
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