| 松花江下游滨岸带典型植被根际土壤细菌群落结构与功能多样性 |
| 摘要点击 2122 全文点击 687 投稿时间：2021-06-29 修订日期：2021-09-23 |
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| 中文关键词 松花江 植被类型 土壤细菌 群落结构 功能预测 |
| 英文关键词 Songhua River vegetation types soil bacteria community diversity function prediction |
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| 中文摘要 |
| 通过分析松花江典型植被（芦苇、白杨、松江柳和修氏苔草）根际土壤细菌群落结构与功能的差异，辨识不同类型植被修复受损滨岸带生境的潜力，为松花江受损滨岸带生境修复和保护提供理论依据.使用Illumina MiSeq PE300二代高通量测序平台对松花江下游滨岸带的4种典型植被根际土壤细菌16S rRNA进行测序，分析不同植被根际土壤细菌群落组成与功能的差异及其影响因素.结果表明，芦苇根际土壤细菌多样性的Ace、Chao1和Shannon指数显著高于白杨（P<0.05），与松江柳和修氏苔草的土壤细菌多样性差异不显著.主坐标轴分析和ANOSIM组间差异检验表明，白杨与其它植被根际土壤细菌群落结构差异显著（P<0.05），松江柳、修氏苔草和芦苇根际土壤细菌群落结构相近.4种典型植被根际土壤细菌共划分为38个菌门，其中，酸杆菌门（Acidobacteria）、放线菌门（Actinobacteria）、绿弯菌门（Chloroflexi）、疣微菌门（Verrucomicrobia）和变形菌门（Proteobacteria）为各样地共有的优势菌门（相对丰度>5%）.硝化螺旋菌门（Nitrospirae）、芽单胞菌门（Gemmatimonadetes）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和己科河菌门（Rokubacteria）的相对丰度均超过1%.4种典型植被根际土壤细菌群落包括6类一级代谢通路，43类二级代谢通路，其中主要的二级代谢通路（功能基因序列相对丰度>1%）包括14类.碳氮比、土壤pH和有机碳为影响不同植被根际土壤细菌多样性的关键环境因子.由此可见，不同植被在修复滨岸带生境中的效果具有一定差异，湿地植被（松江柳和修氏苔草）有利于土壤细菌多样性的提高和土壤生态系统功能的发挥. |
| 英文摘要 |
| The aim of this study was to provide a reference for the riparian zone with protection and ecological restoration by analyzing the differences in typical vegetation (Phragmites communis, Populus tomentosa, Salix sungkianica, and Carex schmidtii) rhizosphere bacterial communities and their functions and identifying the potential of different types of vegetation to restore the damaged riparian zone in Songhua River. The 16S rRNA of rhizosphere soil bacteria in the four typical vegetation types of the riparian zone along the downstream of the Songhua River was sequenced using the Illumina MiSeq PE300 high-throughput sequencing platform. The community diversity, functional differences, and influencing factors of rhizosphere soil bacteria for different vegetation types were analyzed. The results showed that the Ace index, Chao1 index, and Shannon index of soil bacterial diversity in P.communis were significantly higher than those of P.tomentosa (P<0.05), and there was no significant difference between the above two types of vegetation and S. sungkianica and C.schmidtii. There were significant differences between the soil bacterial community structure of P. tomentosa and that of the three other vegetation types (P<0.05). The soil bacterial community structures of S.sungkianica, C.schmidtii, and P.communis were similar. Bacteria in the rhizosphere soil of the four typical vegetation types could be divided into 38 phyla. Acidobacteria, Actinobacteria, Chloroflexi, Verrucomicrobia, and Proteobacteria were the dominant phyla (relative abundance>5%), and the Nitrospirae, Gemmatimonadetes, Bacteroidetes, Firmicutes, and Rokubacteria of bacteria had a relative abundance greater than 1%. The bacterial community in the rhizosphere soil of the four typical vegetation types had 6 primary metabolic pathways and 43 secondary metabolic pathways, including 14 types of main secondary metabolic pathways (relative abundance>1%). Diversity in rhizosphere soil bacterial communities of different vegetation types was significantly influenced by the C/N ratio, soil pH, and moisture content. Hence, the effects of different vegetation types in repairing the degraded riparian zone were different, and wetland vegetation (S.sungkianica and C.schmidtii) was conducive to the improvement in soil bacterial diversity and soil ecosystem functions. |
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