| 高氨氮废水与城市生活污水短程硝化系统菌群比较 |
| 摘要点击 2912 全文点击 1771 投稿时间：2012-06-19 修订日期：2012-10-12 |
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| 中文关键词 短程硝化 生物脱氮 16S rDNA克隆文库 PLFA 定量PCR |
| 英文关键词 partial nitritation biological nitrogen removal 16S rDNA clone library phospholipid fatty acid (PLFA) quantitative PCR |
| 作者 | 单位 | E-mail | | 赵志瑞 | 中国科学院生态环境研究中心, 北京 100085
中国科学院大学, 北京 100049 | zhiruizh@163.com | | 马斌 | 北京城市排水集团有限责任公司, 北京 100022 | | | 张树军 | 北京城市排水集团有限责任公司, 北京 100022 | | | 李滨 | 河北科技大学环境科学与工程学院, 石家庄 050018 | | | 白志辉 | 中国科学院生态环境研究中心, 北京 100085 | zhbai@rcees.ac.cn | | 王晓辉 | 河北科技大学环境科学与工程学院, 石家庄 050018 | | | 庄国强 | 中国科学院生态环境研究中心, 北京 100085 | | | 张洪勋 | 中国科学院生态环境研究中心, 北京 100085
中国科学院大学, 北京 100049 | |
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| 中文摘要 |
| 短程硝化是污水脱氮工艺中的重要环节,系统中的菌群结构决定了其处理效果. 为探讨短程硝化系统中的微生物对不同污水的适应性,利用细菌16S rDNA克隆文库、磷脂脂肪酸(PLFA)和定量PCR分析方法对高氨氮废水和城市生活污水短程硝化系统中活性污泥的细菌群落结构、总体微生物的多样性以及功能微生物进行了比较. 克隆文库结果表明两个系统中细菌群落结构明显不同,城市生活污水中细菌种类更丰富,但两个系统的优势菌群都属于变形菌门(Proteobacteria)和拟杆菌门(Bacteroidete). 磷脂脂肪酸分析结果显示高氨氮废水短程硝化系统中微生物多样性指数和均匀度指数明显低于城市生活污水. 定量PCR结果表明,高氨氮废水短程硝化系统中氨氧化菌(AOB)与亚硝酸盐氧化菌(NOB)的数量都多于城市生活污水短程硝化系统; 高氨氮废水短程硝化系统中AOB比NOB高3个数量级,而城市生活污水短程硝化系统中AOB比NOB高2个数量级. |
| 英文摘要 |
| Nitritation is an important part of the biological nitrogen removal process, and the performance of the process was determined by the microbial community structure. To explore the microbial adaptability to different sewage, the microbial diversity and the amount of bacteria were investigated in a high ammonia wastewater treatment process and a sewage treatment process using the clone library of bacterial 16S rDNA, the phospholipid fatty acid method (PLFA) and the quantitative PCR. The clone library results showed that there was a significantly difference in bacterial community structure of these two processes, although the dominant bacteria belong to the Proteobacteria and Bacteroidete, there were more clusters in the sewage treatment process. The PLFAs results showed that the microbial diversity index and the evenness index of the high ammonium wastewater treatment process were significantly low. The quantitative PCR results showed that amounts of ammonia oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the high ammonium wastewater treatment process were higher than these in sewage treatment process. The copy number of AOB was higher than the copy number of NOB in the high ammonia wastewater treatment process by three orders magnitude. The copy number of AOB was higher than the copy number of NOB in sewage treatment process by two orders of magnitude. |
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