Profile and removal of endocrine disrupting chemicals by using an ER/ARcompetitive ligand binding assay and chemical analyses
Ze-hua Liu , Mamoru Ito , Yoshinori Kanjo , Atsushi Yamamoto
DOI:
Received July 09, 2008,Revised December 31, 2008, Accepted , Available online
Volume 21,2009,Pages 900-906
- Summary
- References
- Related Articles
An estrogen receptor (ER)/androgen receptor (AR) ligand competitive binding assay (ER/AR-binding assay) and chemical analyses
were used to evaluate the endocrine disrupting chemicals (EDCs) behavior of two municipal wastewater treatment plants (WWTPs) (K
and S). In the influents, estrone (E1), androsterone (A), androstenedione (AD), BPA (bisphenol A), NP (nonylphenol) and daidzein (DZ)
were detected in high amounts with subsequent 24 h-average concentrations of 350, 1000, 29, 1300, 3900, and 5700 ng/L in K-WWTP
and of 310, 620, 59, 1600, 2600, and 8400 ng/L in S-WWTP. The estrogenic (androgenic) activity as 17 -estradiol (E2) equivalents
(EEQ) or testosterone (Te) equivalents (TEQ) was consequently 620 ng E2/L (570 ng Te/L) and 580 ng E2/L (800 ng Te/L) for the
two WWTPs. The removal e ciencies of the above mentioned sole target chemicals were 51%–100% for K-WWTP and 55.6%–100%
for S-WWTP. The removal e ciencies of EEQ were about 73% for both WWTPs, while the removal e ciencies of TEQ were 62.1%
for K-WWTP and 98.4% for S-WWTP. In addition, chemical-derived EEQ were about 1.2%–52.4% of those by ER-binding assay for
K-WWTP and the corresponding ratios were 1.3%–83.3% for S-WWTP, while chemical derived TEQ were less than 3% of values
measured by the AR-binding assay for both WWTPs.
