Effects of chlortetracycline, Cu and their combination on the performance and microbial community dynamics in swine manure anaerobic digestion
Rui Wang , Junya Zhang , Jibao Liu , Dawei Yu , Hui Zhong , Yawei Wang , Meixue Chen , Juan Tong , Yuansong Wei
DOI:10.1016/j.jes.2017.08.023
Received January 22, 2017,Revised July 14, 2017, Accepted August 29, 2017, Available online September 08, 2017
Volume 30,2018,Pages 206-215
Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline (CTC), Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2 L anaerobic digestion reactors were established including reactor A (control), B (CTC spiked by 0.5 g/kg dry weight, dw), C (Cu spiked by 5 g/kg dw) and D (combination of CTC, 0.5 g/kg dw, and Cu, 5 g/kg dw), and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by 21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion (by 30.3%). Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C.
