摘要:
为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500 μg·L-1Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40 μg·L-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8 μg·L-1时即可显著抑制HSP70 mRNA表达量,并在40 μg·L-1时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500 μg·L-1时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。
Abstract:
In order to understand the effects of heavy metal pollution on the gene expression of heat shock proteins (HSPs) in marine fish, Sebastiscus marmoratus were exposed to different concentrations (i.e., 1.6, 8, 40, 200, 500 μg·L-1) of Cd or Pb for 5 days. A quantitative loop-mediated isothermal amplification (LAMP) method was used to analyze the hepatic mRNA levels of HSP60, HSP70, HSP90, and HSC70. Under the Pb exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly reduced except in the 40 μg·L-1 treatment, whereas the mRNA levels of HSP70 were significantly decreased by 8 μg·L-1 Pb, and reached the minimum when Pb was 40 μg·L-1. Under the Cd exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly affected;in contrast, mRNA levels of HSP70 were significantly increased and peaked at 500 μg·L-1 Cd. In conclusion, the hepatic HSP70 was relatively more sensitive to heavy metal exposure than the other HSPs, and thus had the potential to be the early warning for heavy metal pollution in marine environment.
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