摘要:
以泥鳅为受试动物,采用急性毒性、微核试验及酶活分析实验,研究了离子液体[C16mim]Cl对水生生物的生物毒性作用。急性实验表明:[C16mim]Cl对泥鳅有明显的毒性,其对泥鳅24、48和96 h的LC50分别为2.533、1.763和1.379 mg · L-1;微核实验表明:对照组红细胞微核率为0.5‰左右,而处理组红细胞微核率最高达到12.89‰,且各处理组均明显高于对照组(P <0.05);酶活分析实验表明:处理组血清的GPT和GOT活力均明显高于对照组,且随[C16mim]Cl浓度增高而呈现上升趋势。从以上结果可以得出:[C16 mim]Cl对泥鳅具有显著的遗传毒性和生理毒性效应,因而推测其大量使用可能对水生生物及生态环境具有一定的毒害作用。
Abstract:
The toxicity effect of[C16mim]Cl was explored using the loach as tested animal based on the acute toxicity, micronucleus (MN) and enzyme assay test. The acute test result showed that[C16mim]Cl had evident toxicity effect on loach, which LC50 of 24,48 and 96 h were 2.533, 1.763 and 1.379 mg·L-1, respectively. The results of MN test indicated that the MN frequency of control group just only was 0.5‰, while the highest frequency of MN was 12.89‰ in treating group, and there was significant difference between the treatment groups and the control group (P < 0.05). In enzyme assay test, the results indicated that the GPT and GOT activity of blood serum in treatment groups were significantly higher than that of control fish (P < 0.05), which increased with the increasing of treating concentration and time of[C16mim]Cl ion liquid. It was concluded that[C16 mim]Cl had significant genetic toxicity and physiological toxicity effect on loach, suggesting that the use of[C16 mim]Cl in industry may have the toxic effect on aquatic organisms and ecosystem.
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