| 1株汞挥发真菌的分离及特性分析 |
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| 引用本文： | 司光正,杨清晨,董佳,闫天歌,常军军,陈金全.1株汞挥发真菌的分离及特性分析[J].环境工程,2020,38(9):247-252. |
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| 作者姓名： | 司光正 杨清晨 董佳 闫天歌 常军军 陈金全 |
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| 作者单位： | 1. 云南大学 生态学与环境学院 高原山地生态与退化环境修复重点实验室, 昆明 650091; |
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| 基金项目： | 国家重点实验室开放基金;云南省教育厅项目 |
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| 摘 要： | 近年来土壤汞污染日益严峻，严重危害环境和人类健康，已成为一个世界性的环境问题，因此选择合理有效的方法对其进行修复与控制刻不容缓。有些微生物对汞具有吸附和挥发的特性，可用于受汞污染环境的修复。实验从受重金属污染的土壤中分离纯化出1株高抗汞真菌（DC-F9），其汞的最小抑菌浓度（MIC）达到160 mg/L；通过ITS序列比较，鉴别该菌属于曲霉属真菌；摇瓶培养发现，该真菌在Hg（Ⅱ）浓度为5 mg/L的培养基中对汞的挥发率、吸附率和总去除率分别为36.8%、58.4%、95.2%；而在Hg（Ⅱ）浓度为10 mg/L的培养基中，其值分别为45.4%、40.2%、85.6%；通过FTIR分析，发现该菌株为响应汞的胁迫表现出多种生理上变化。结果表明该菌株具有对汞污染环境修复的应用潜力。
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| 关 键 词： | 土壤汞污染 汞挥发 真菌 吸附 |
| 收稿时间： | 2019-07-09 |
ISOLATION AND CHARACTERIZATION OF A STAIN OF MERCURY VOLATIZING FUNGUS |
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| SI Guang-zheng,YANG Qing-chen,DONG Jia,YAN Tian-ge,CHANG Jun-jun,CHEN Jin-quan.ISOLATION AND CHARACTERIZATION OF A STAIN OF MERCURY VOLATIZING FUNGUS[J].Environmental Engineering,2020,38(9):247-252. |
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| Authors: | SI Guang-zheng YANG Qing-chen DONG Jia YAN Tian-ge CHANG Jun-jun CHEN Jin-quan |
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| Affiliation: | 1. Key Laboratory of Plateau Mountain Ecology and Degraded Environment Rehabilitation, College of Ecology and Environment, Yunnan University, Kunming 650091, China;2. Institute of International Rivers and Eco-Security, Yunnan University, Kunming 650091, China |
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| Abstract: | In recent years, soil contaminated with mercury (Hg) has become serious problem on earth, endangering the environment and human health. Therefore, it is urgent to develop a reasonable and effective method to remediate it. Some microorganisms have capacity of adsorption and volatilization for Hg(Ⅱ), and can be applied to the bioremediation of Hg-contaminated environments. In this study, a highly Hg-resistant fungus (DC-F9) was isolated from the heavy metal-contaminated soil with a minimum inhibitory concentration (MIC) of 160 mg/L. The strain belonged to the genus Aspergillus by the phylogenetic analysis of its ITS sequence. In the medium supplemented with Hg(Ⅱ) concentration of 5 mg/L, the volatilization rate, adsorption rate and total removal rate of Hg(Ⅱ) were 36.8%, 58.4%, 95.2% respectively; and they were 45.4%, 40.2%, 85.6% respectively at Hg(Ⅱ) concentration of 10 mg/L. The strain exhibited a variety of physiological changes in response to mercury stress by FTIR analyses. These results showed that the DC-F9 had potentials of bioremediation for Hg-contaminated environment. |
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