| 三江平原不同类型小叶章湿地土壤细菌群落功能多样性 |
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| 引用本文： | 隋心,张荣涛,杨立宾,许楠,钟海秀,王继丰,倪红伟.三江平原不同类型小叶章湿地土壤细菌群落功能多样性[J].环境科学研究,2016,29(10):1479-1486. |
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| 作者姓名： | 隋心 张荣涛 杨立宾 许楠 钟海秀 王继丰 倪红伟 |
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| 作者单位： | 1.黑龙江省科学院自然与生态研究所, 湿地与生态保育国家地方联合工程实验室, 黑龙江 哈尔滨 150040 |
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| 基金项目： | 国家自然科学基金项目(31470019,31400429)；黑龙江省院所财政应用技术专项(STJB16-01) |
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| 摘 要： | 为全面了解三江平原小叶章湿地土壤细菌功能多样性的变化规律,采用Biolog-ECO微平板法对三江平原内不同类型小叶章湿地(草甸化小叶章湿地、沼泽化草甸小叶章湿地和沼泽化小叶章湿地)的土壤细菌群落功能多样性变化规律和特点进行分析.结果表明:不同类型小叶章湿地土壤理化性质差异显著(P<0.05).土壤细菌群落平均颜色变化率(AWCD)随培养时间的延长而增加,不同类型湿地土壤细菌群落代谢活性表现为草甸化小叶章湿地>沼泽化草甸小叶章湿地>沼泽化小叶章湿地；土壤细菌群落多样性之间差异显著(P<0.05),草甸小叶章湿地土壤微生物的Shannon-Wiener多样性指数和McIntosh指数最高,分别为3.30和64.03；沼泽化草甸小叶章湿地次之,分别为3.23和60.63；沼泽化小叶章湿地最低,分别为3.19和54.21.Shannon-Wiener多样性指数和McIntosh指数与平均颜色变化率变化规律一致,说明土壤细菌群落结构受到土壤理化性质的影响,并且随着土壤含水量的升高,土壤细菌群落的平均颜色变化率和多样性均呈下降趋势.土壤细菌群落对不同碳源的利用强度上存在明显差异,其中对碳水化合物类、氨基酸类碳源的利用强度差异不大(P>0.05),但是对羧酸类、胺类、酚酸类、多聚物类碳源的利用强度差异显著(P<0.05).主成分分析(PCA)表明,两个主成分累计贡献率为80%,能够很好地解释不同湿地土壤细菌在碳源利用上有明显的空间分异这一现象.碳水化合物类碳源对土壤细菌群落多样性的影响最高,其次是氨基酸类和羧酸类碳源.研究显示,土壤理化性质及植被群落组成是影响小叶章湿地土壤细菌群落组成和功能活性的重要因素.
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| 关 键 词： | 小叶章湿地 土壤微生物 功能多样性 碳代谢 |
| 收稿时间： | 2016/1/29 0:00:00 |
| 修稿时间： | 2016/6/12 0:00:00 |
Bacterial Community Functional Diversity of Calamagrostis angustifolia Wetland in Sanjiang Plain |
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| Affiliation: | 1.National and Provincial Joint Engineering Laboratory of Wetlands and Ecological Conservation,Institute of Nature & Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China2.National and Provincial Joint Engineering Laboratory of Wetlands and Ecological Conservation,Institute of Nature & Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China ;College of Geographical Science, Harbin Normal University, Harbin 150025, China |
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| Abstract: | To understand the bacterial functional diversity regarding soil carbon metabolism in Calamagrostis angustifolia wetlands, the topsoils(0-20 cm) of three different C.angustifolia wetlands were collected in Sanjiang Plain. The distribution and variation of carbon metabolism diversity were assessed by Biolog-ECO microplate method. The results showed that the soil physicochemical properties were significantly different between the three wetlands(P<0.05). The average well color development(AWCD) of all the samples increased with the extension of incubation time, with the values of AWCD following the decreasing order: meadow wetlands>marsh meadow wetlands>marsh wetlands. The soil bacterial diversities between the wetlands were significantly different, and the Shannon-Wiener and McIntosh diversity indexes also showed the same trend as AWCD. The meadow wetland had the greatest Shannon-Wiener and McIntosh indexes, 3.30 and 64.03； the second was marsh meadow wetland, 3.23 and 60.63； the lowest was marsh wetland, 3.19 and 54.21, respectively. The Shannon-Wiener and McIntosh diversity indexes also showed the same trends as AWCD. The bacterial community structures were influenced by soil physicochemical properties, with the AWCD and diversity indexes decreasing with the increase of soil water content. The utilization intensity of six categories of carbon sources by soil bacteria had significant differences, but not for carbohydrates or amino acids(P>0.05). Principal component analysis(PCA), of which the cumulative contribution was 80%, could well explain that soil bacterial carbon utilization had obvious spatial variation, suggesting the bacterial metabolism diversity could be reflected in the use of carbohydrates, amino acids and carboxylic acids, particularly carbohydrates. In addition, soil physicochemical properties and vegetation composition were the main factors modifying soil bacterial composition and functional activity in C.angustifolia wetlands. |
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