Pseudomonas sp. (Strain 10–1B): A potential inoculum candidate for green and sustainable remediation |
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| Authors: | Maryam Bello‐Akinosho Rasheed Adeleke Mapitsi S Thantsha Mphekgo Maila |
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| Affiliation: | 1. New Agricultural Sciences Building, University of Pretoria, Lunnon Road, Pretoria 0083, South Africa;2. Agricultural Research Council-Institute for Soil, Climate and Water, 600 Belvedere Street, Arcadia, Pretoria 0001, South Africa |
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| Abstract: | Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is a consequence of various industrial processes which destabilizes the ecosystem. Bioremediation by bacteria is a cost‐effective and environmentally safe solution for reducing or eliminating pollutants in soils. In the present study, we artificially polluted agricultural soil with used automobile engine oil with a high PAH content and then isolated bacteria from the soil after 10 weeks. Pseudomonas sp. strain 10–1B was isolated from the bacterial community that endured this artificial pollution. We sequenced its genomic DNA on Illumina MiSeq sequencer and evaluated its ability to solubilize phosphate, fix atmospheric nitrogen, and produce indoleacetic acid, in vitro, to ascertain its potential for contribution to soil fertility. Its genome annotation predicted several dioxygenases, reductases, ferredoxin, and Rieske proteins important in the ring hydroxylation initiating PAH degradation. The strain was positive for the soil fertility attributes evaluated. Such combination of attributes is important for any potential bacterium partaking in sustainable bioremediation of PAH‐polluted soil. |
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