| 泥膜混合MBBR系统自养脱氮工艺的启动研究 |
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| 引用本文： | 姚丽婷,陈启智,赖勇州,梁瑜海.泥膜混合MBBR系统自养脱氮工艺的启动研究[J].环境科学研究,2022,35(4):999-1006. |
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| 作者姓名： | 姚丽婷 陈启智 赖勇州 梁瑜海 |
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| 作者单位： | 1.华南农业大学资源环境学院，广东 广州 510642 |
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| 基金项目： | 广东省自然科学基金-面上项目(No.2021A1515010904)~~； |
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| 摘 要： | 为了加速厌氧氨氧化菌(AnAOB)富集，解决自养脱氮工艺启动缓慢的问题，在短程硝化絮状污泥反应器中投加含有少量AnAOB的悬浮填料，构建泥膜混合移动床生物膜反应器(MBBR)系统，探讨该系统在自养脱氮启动中的作用. 结果表明:①在温度为20~30 ℃、pH为7.8~8.2、DO浓度为0.2~0.9 mg/L的条件下，经45 d的运行，成功富集AnAOB. 通过调整运行模式和曝气量，TN去除率提高至70%左右，成功启动自养脱氮工艺. ②在运行过程中，曝气阶段主要发生短程硝化反应，缺氧阶段主要发生厌氧氨氧化反应. ③泥膜混合MBBR系统中优势的好氧氨氧化菌(AOB)和AnAOB分别为Nitrosomonas和Candidatus_Kuenenia. Nitrosomonas主要分布于絮状污泥中，其相对丰度从42.95%减至30.98%；而Candidatus_Kuenenia主要分布于填料生物膜中，其相对丰度从5.88%增至25.90%. ④泥膜混合MBBR系统中还检测出Ignavibacteriales_bacterium_UTCHB1、Pseudomonas、Denitratisoma等多种反硝化细菌，说明部分TN损失是通过内源反硝化途径实现. 研究显示，基于短程硝化絮状污泥的泥膜混合MBBR系统，可以维持稳定的短程硝化，快速富集AnAOB，也可以有效缩短自养脱氮工艺的启动时间.
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| 关 键 词： | 自养脱氮 移动床生物膜反应器(MBBR) 好氧氨氧化菌(AOB) 厌氧氨氧化菌(AnAOB) 微生物群落 |
| 收稿时间： | 2021-06-19 |
Start-Up of Autotrophic Nitrogen Removal Process in A Sludge-Biofilm Hybrid MBBR System |
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| YAO Liting,CHEN Qizhi,LAI Yongzhou,LIANG Yuhai.Start-Up of Autotrophic Nitrogen Removal Process in A Sludge-Biofilm Hybrid MBBR System[J].Research of Environmental Sciences,2022,35(4):999-1006. |
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| Authors: | YAO Liting CHEN Qizhi LAI Yongzhou LIANG Yuhai |
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| Affiliation: | 1.College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China2.Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | In order to accelerate the enrichment of anaerobic ammonia oxidizing bacteria (AnAOB) and solve the problem of slow start-up of autotrophic nitrogen removal process, this study constructed a sludge-biofilm hybrid moving bed biofilm reactor (MBBR) by adding suspended filler containing a small amount of AnAOB to a partial nitrification flocculation sludge reactor. The role of the system in the start-up of autotrophic nitrogen removal process was investigated. The results show that: (1) After 45 days of operation, AnAOB was enriched successfully under the conditions of temperature (20-30 ℃), pH (7.8-8.2) and DO concentration (0.2-0.9 mg/L). The total nitrogen (TN) removal rate was increased to about 70% by adjusting the operation mode and aeration rate, and the start-up of autotrophic nitrogen removal process was realized. (2) During operation, partial nitrification mainly occurs in the aeration stage, and anammox mainly occurred in the anoxic stage. (3) The dominant genera of ammonia oxidizing-bacteria (AOB) and AnAOB were Nitrosomonas and Candidatus_Kuenenia, respectively. Nitrosomonas was mainly distributed in the flocculated sludge, and the relative abundance decreased from 42.95% to 30.98%. While Candidatus_Kuenenia was mainly distributed in the filler biofilm, and its relative abundance increased from 5.88% to 25.90%. (4) The system also detected Ignavibacteriales_bacterium_UTCHB1, Pseudomonas, Denitratisoma and other denitrifying bacteria, indicating that part of the TN loss was achieved through endogenous denitrification. This study shows that the sludge-biofilm hybrid MBBR based on the partial nitrification flocculation sludge can maintain stable partial nitrification and rapidly enrich AnAOB, which can effectively shorten the start-up time of the autotrophic nitrogen removal process. |
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