| 一株高效嗜碱反硝化菌的分离鉴定及其脱氮特性研究 |
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| 引用本文： | 韩冰,丁雷,史沐,周建民,袁明珠,任颖,李爽,崔长征.一株高效嗜碱反硝化菌的分离鉴定及其脱氮特性研究[J].环境科学研究,2020,33(9):2129-2137. |
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| 作者姓名： | 韩冰 丁雷 史沐 周建民 袁明珠 任颖 李爽 崔长征 |
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| 作者单位： | 华东理工大学资源与环境工程学院, 高浓度难生物降解有机废水处理技术国家工程实验室, 上海 200237 |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目（No.B100-C-1503）；上海市重点学科建设项目资助（No.B506） |
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| 摘 要： | 为了应对日趋严苛的废水TN排放标准要求，试验采用溴百里酚蓝（BTB）培养基，从某煤化工废水处理厂反硝化缺氧池活性污泥中，经多次分离、纯化获得了一株高效兼性厌氧反硝化菌株HK13.通过形态观察及16S rRNA基因序列分析，菌株HK13被鉴定为施氏假单胞菌属（Pseudomonas stutzeri）.在此基础上，利用含硝酸盐模拟废水，探讨了碳源类型、C/N（碳氮比）、初始pH、溶解氧（以不同摇床转速表征不同浓度的溶解氧）和培养温度对菌株HK13反硝化脱氮能力的影响，确定了该菌株的最优生长条件和最大脱氮效率.结果表明:①菌株HK13最适反硝化脱氮条件为以柠檬酸钠碳源，C/N 8，培养温度35℃，初始pH 8~10，摇床转速100 r/min.②初始ρ（NO3--N）为106.67 mg/L下，反应12 h后菌株HK13对TN的去除率可达92.62%；反应9~12 h时，该菌株的脱氮速率最高，可达20.03 mg/（L·h），其16 h的脱氮率在98%以上，且无亚硝酸盐积累.③菌株HK13适宜生长的温度和pH范围广泛，分别为20~40℃和7~10.研究显示，菌株HK13具有快速高效的脱氮能力及嗜碱特性，拓宽了生物脱氮工艺对环境条件的适用范围，在废水脱氮领域具有广泛的应用前景.
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| 关 键 词： | 反硝化脱氮 施氏假单胞菌属 嗜碱 16S rRNA 亚硝酸盐 |
| 收稿时间： | 2019/10/7 0:00:00 |
| 修稿时间： | 2019/11/22 0:00:00 |
Identification and Characterization of an Alkalophilic Pseudomonas stutzeri Strain with High Denitrification Capacity |
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| HAN Bing,DING Lei,SHI Mu,ZHOU Jianmin,YUAN Mingzhu,REN Ying,LI Shuang,CUI Changzheng.Identification and Characterization of an Alkalophilic Pseudomonas stutzeri Strain with High Denitrification Capacity[J].Research of Environmental Sciences,2020,33(9):2129-2137. |
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| Authors: | HAN Bing DING Lei SHI Mu ZHOU Jianmin YUAN Mingzhu REN Ying LI Shuang CUI Changzheng |
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| Affiliation: | National Engineering Laboratory for Industrial Wastewater Treatment, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | In order to meet increasingly strict effluent TN discharge standards, a high efficient anaerobic denitrifier HK13 was isolated and purified with the modified bromothymol blue (BTB) medium from the anoxic activated sludge in a coal chemical wastewater treatment plant. Based on morphological observation and 16S rRNA gene sequence analysis, strain HK13 was identified as Pseudomonas stutzeri. The effects of carbon source, C/N ratio (carbon to nitrogen ratio), initial pH, dissolved oxygen (dissolved oxygen was characterized by shaker speed) and temperature on the denitrification characteristics of strain HK13 were studied using simulated nitrate wastewater, and the optimum growth conditions and the maximum nitrogen removal efficiency were determined. The results showed that: (1) The optimum denitrification conditions for strain HK13 were sodium citrate as the carbon source, C/N 8, 35 ℃, initial pH 8-10, and shaker speed 100 r/min. (2) Under the optimum conditions of initial ρ(NO3--N) of 106.67 mg/L, the TN removal efficiency of strain HK13 was 92.62% at 12 h. The peak TN removal rate of this strain reached 20.03 mg/(L·h) between 9 h and 12 h, and the TN removal efficiency was higher than 98% at 16 h without nitrite accumulation in the system. (3) The suitable temperature and pH range for strain HK13 were 20-40 ℃ and 7-10, respectively. These results demonstrated that strain HK13 exhibited efficient denitrification ability with alkalophilic feature, which broadened the environmental conditions of the biological denitrification process for the treatment of nitrogen polluted wastewater. |
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| Keywords: | denitrification Pseudomonas stutzer alkalophilic 16S rRNA nitrite |
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