| 固定化好氧脱氮菌及其在低C/N鸭场废水处理中的应用 |
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| 引用本文： | 李宇馨,陈鹏程,廖劲松,戴睿智,潘圳钟,杨德,许燕滨.固定化好氧脱氮菌及其在低C/N鸭场废水处理中的应用[J].环境科学研究,2021,34(2):328-336. |
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| 作者姓名： | 李宇馨 陈鹏程 廖劲松 戴睿智 潘圳钟 杨德 许燕滨 |
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| 作者单位： | 1.广东工业大学环境科学与工程学院, 广东 广州 510006 |
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| 基金项目： | 广东省重点领域研发计划项目（No.2019B110205004）；广东省基础与应用基础研究基金项目（No.2019B1515210033）；广东省科技计划项目（No.2016B020240003） |
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| 摘 要： | 为解决集约化畜禽养殖废水处理工艺（厌氧-缺氧/好氧-缺氧/好氧）中普遍存在的出水NO3--N浓度超标的问题，试验将一株具有高效去除NO3--N能力的好氧细菌Pseudomonas mendocina LYX固定于丝瓜络，并制备成菌剂，研究其在最佳脱氮条件下对鸭场废水深度处理的强化效果.结果表明:①在C/N为5、培养温度为35℃、pH为8、ρ（DO）为3.45 mg/L的条件下，P.mendocina LYX达到最优脱氮效果.②固定化菌剂投加后，反应器中NO3--N和CODCr的去除率较菌剂强化前分别提高了40%和20%，ρ（NO2--N）提前达到峰值，反硝化进程被明显推进.③反应器运行至第17天时，污泥中好氧脱氮菌Pseudomonas的相对丰度由最初的0.03%升至8.50%，反应器内微生物多样性显著增加，此时，NO3--N和CODCr去除率均达到最大值，分别为86%和79%.④反应器运行至第22天时，污泥中Pseudomonas的相对丰度降至0.59%，菌剂强化作用逐渐减弱，反应器中NO3--N和CODCr去除率基本与菌剂强化前持平，菌剂强化效果具有周期性.研究显示，固定化P.mendocina LYX菌剂有望在好氧条件下通过原位强化解决低C/N水质中的NO3--N污染问题，具有较好的应用前景.
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| 关 键 词： | Pseudomonas mendocina LYX 脱氮菌剂 低C/N 鸭场废水 |
| 收稿时间： | 2019/12/9 0:00:00 |
| 修稿时间： | 2020/5/1 0:00:00 |
Immobilization of Aerobic Denitrifying Bacterium and Its Application in Treatment of Duck Farm Wastewater with Low C/N |
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| LI Yuxin,CHEN Pengcheng,LIAO Jinsong,DAI Ruizhi,PAN Zhenzhong,YANG De,XU Yanbin.Immobilization of Aerobic Denitrifying Bacterium and Its Application in Treatment of Duck Farm Wastewater with Low C/N[J].Research of Environmental Sciences,2021,34(2):328-336. |
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| Authors: | LI Yuxin CHEN Pengcheng LIAO Jinsong DAI Ruizhi PAN Zhenzhong YANG De XU Yanbin |
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| Affiliation: | 1.School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China2.Guangdong Yikangsheng Environmental Science and Technology Company Limited, Yunfu 527300, China |
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| Abstract: | Intensive livestock and poultry breeding wastewater treatment processes (anoxic-anaerobic/oxic-anaerobic/oxic) generally have the problem that the nitrate concentration in the effluent exceeds the standard. In order to solve this problem, the bacterium Pseudomonas mendocina LYX, which can efficiently remove nitrate under aerobic conditions, was prepared as the microbial agent and fixed on loofah to form a biofilm, and then was used in the advanced treatment of the wastewater from some duck farm. The results showed that:(1) The optimal nitrogen removal conditions of P. mendocina LYX were C/N 5, 35℃, pH 8 and DO 3.45 mg/L. (2) The addition of the microbial agent in simulated wastewater treatment system could increase the removal rates of NO3--N and CODCr in the duck farm wastewater by 40% and 20%, respectively. The NO2--N concentration peak in the reactor appeared earlier than the control, which indicated that the denitrification process was accelerated significantly by P. mendocina LYX. (3) The relative abundance of the aerobic denitrifying bacteria Pseudomonas in the reactor increased from 0.03% to 8.50% at the 17th day, and the microbial diversity in the system also increased significantly. Meanwhile, the removal rates of nitrate and CODCr also increased to 86% and 79%, respectively. (4) When the reactor was operated to the 22nd day, the relative abundance of Pseudomonas dropped to 0.59%, which resulted in the gradual weakening of the enhancement effect of the agent, and the removal efficiencies of nitrate and CODCr also decreased to a level comparable to that of the control. It indicated that the strengthening effect of the inoculum was periodic. Therefore, P. mendocina LYX can be a promising inoculum in solving the problem of NO3--N accumulation in aerobic tank of wastewater treatment system with low C/N by adding in situ under aerobic conditions, and has a broad prospect in application. |
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| Keywords: | Pseudomonas mendocina LYX microbial agent for nitrate removal low C/N ratio duck farm wastewater |
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