| 铁矿区重金属污染对土壤微生物群落变化的影响 |
| |
| 引用本文： | 邢 奕,司艳晓,洪 晨,李 洋.铁矿区重金属污染对土壤微生物群落变化的影响[J].环境科学研究,2013,26(11):1201-1211. |
| |
| 作者姓名： | 邢 奕 司艳晓 洪 晨 李 洋 |
| |
| 作者单位： | 北京科技大学土木与环境工程学院, 北京 100083 |
| |
| 基金项目： | 国家自然科学基金项目(41273091)；北京市科技新星计划项目(Z111106054511043) |
| |
| 摘 要： | 以密云水库上游某铁矿区为研究对象,采用荧光定量PCR研究了矿区内不同采样点土壤中细菌、真菌、放线菌基因数量的变化,并结合土壤的理化性质与重金属污染情况进行了相关分析. 结果表明,研究区微生物的基因数量与该地区的w(AP)(AP为有效磷)均呈显著正相关(P<0.05)；细菌、放线菌的基因数量与该地区的内梅罗污染指数呈显著负相关〔二者R分别为-0.756(P<0.01)和-0.614(P<0.05)〕,而真菌的基因数量与内梅罗污染指数无显著相关性. 采用PCR-DGGE研究了细菌、真菌、放线菌的群落结构变化,冗余度分析结果表明,内梅罗污染指数对细菌、放线菌的种群分布影响较大(P<0.05),对真菌的影响则较小. 细菌、放线菌的种群多样性水平随着污染程度升高呈先升后降的趋势. 微生物数量和结构的变化都表明,不同类群的微生物对重金属敏感程度为真菌抗性最强,放线菌和细菌次之. Cd和Cu污染抑制了土壤中微生物的数量和群落多样性,而轻度Cr、Pb和Zn污染则促进了群落数量的增加和群落结构多样性的丰富.
|
| 关 键 词： | 微生物群落 DGGE 荧光定量 PCR |
| 收稿时间： | 2013/6/18 0:00:00 |
| 修稿时间： | 2013/9/7 0:00:00 |
Impact of Long-Term Heavy Metal Pollution on Microbial Community in Iron Mine Soil |
| |
| XING Yi,SI Yan-xiao,HONG Chen and LI Yang.Impact of Long-Term Heavy Metal Pollution on Microbial Community in Iron Mine Soil[J].Research of Environmental Sciences,2013,26(11):1201-1211. |
| |
| Authors: | XING Yi SI Yan-xiao HONG Chen and LI Yang |
| |
| Affiliation: | Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083, China |
| |
| Abstract: | To examine the spatial changes of microbial community in an iron mine of the upstream area of Miyun Reservoir, soil samples were collected from twelve sites. Quantification of bacteria, fungi and actinobacteria were determined by real-time PCR. Derived from Pearson correlation analysis showed that there was significant positive correlation (P<0.05) between the abundance of total microbes and w(AP). Meanwhile, the abundance of bacteria and actinobacteria had significant negative correlation with contamination index(R=-0.756, P<0.01; R=-0.614, P<0.05). Pollution levels, however, had no effect on fungi abundance. PCR-DGGE was used to analyze the bacteria, fungi, and actinobacteria community structure as well. Redundancy analysis demonstrated that contamination index greatly influenced on bacteria and actinobacteria community (P<0.05) and less influenced on fungi. The diversity of bacteria and actinobacteria increased from low to moderate levels of contamination and then decreased at relatively high contamination index. The changes of microbial abundance and structure revealed that different microbial communities exhibit various responses to heavy metals. Fungi were the most tolerated species to heavy metals, followed by actinobacteria and bacteria in the same contaminant grade. Moreover, Cr, Pb and Zn promoted the abundance and the diversity of microbial community in their moderate pollution. The distribution of microbial community, however, was inhibited by Cd and Cu. This indicated that microbial communities would shift from sensitive population to tolerant population, and it was important to further understand their synergic relationship and biogeochemical processes. |
| |
| Keywords: | microbial DGGE real-time PCR |
|
| 点击此处可从《环境科学研究》浏览原始摘要信息 |
|
点击此处可从《环境科学研究》下载全文 |
|
