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污泥好氧堆肥对PAHs的处理效果和抗生素及抗性基因消解效果
引用本文:孙伟,匡科,严兴,李碧清,雷芳,王双,肖先念.污泥好氧堆肥对PAHs的处理效果和抗生素及抗性基因消解效果[J].环境科学研究,2021,34(7):1757-1763.
作者姓名:孙伟  匡科  严兴  李碧清  雷芳  王双  肖先念
作者单位:广州市净水有限公司,广东 广州 510163
基金项目:广州市科技计划重点项目201710010007广东省重点领域研发计划项目2019B110209002广州市“岭南英杰工程”人才计划[2019] 928 of Guangzhou Human Resources and Social Security Bureau
摘    要:为探讨污泥共堆肥对PAHs的处理效果以及堆肥过程中抗生素及抗性基因的变化情况,试验设置添加辅料木屑、蘑菇渣、微生物发酵菌和酸化生物质炭进行好氧共堆肥处理.结果表明:污泥堆肥对PAHs的去除率基本都在50%以上,特别是芴、菲和蒽的去除率超过了80%.∑16PAHs(16种PAHs总量)的去除率达到78.7%,∑PAHs-cancer(具有致癌活性的4~6环PAHs总量)去除率达到74.1%,∑PAHs-ppc(被我国列入环境“优先污染物”的PAHs总量)去除率达到68.6%.136种抗性基因在堆肥过程中出现了不同程度的消减,其中,BL2a_1(青霉素)、catB5(氯霉素)和tetPA(四环素)等11种抗性基因在堆肥过程中完全消减,消减率达到100%,BL1_asba(头孢霉菌素)、EreA(红霉素)、QnrB(氟喹诺酮)和cmL_e8(氯霉素)等19种抗性基因在堆肥过程中消减率均超过85%,堆肥过程能有效消减该类抗性基因.研究显示,抗生素及其降解产物影响了微生物群落属水平,分枝杆菌属和鞘氨醇杆菌属变化明显,污泥堆肥产品施用过程中抗性基因出现水平迁移,不宜直接农业施用. 

关 键 词:污泥堆肥  微生物发酵菌  PAHs  抗性基因  消减率
收稿时间:2020-08-11

Sludge Aerobic Composting to Treat PAHs,Antibiotics and Resistance Genes
Affiliation:Guangzhou Sewage Purification Co., Ltd., Guangzhou 510163, China
Abstract:In order to study the effect of sludge composting on PAHs and the changes of antibiotics and resistance genes in the composting process, experiments were set up to add auxiliary sawdust, mushroom residue, microbial fermentation bacteria and acidified biochar. The results show that the degradation rate of PAHs by sludge composting was more than 50%, and the degradation rate of fluorene, phenanthrene and anthracene was more than 80%. The total degradation rate of ∑16PAHs (total amount of 16 polycyclic aromatic hydrocarbons) was 78.7%, that of ∑PAHs-cancer(total amount of 4-6-ring polycyclic aromatic hydrocarbons with carcinogenic activity) was 74.1%, and that of ∑PAHs-PPC(total PAHs listed as 'priority pollutants' in China) was 68.6%, respectively. During the composting, the contents of 136 resistant genes were reduced to different degrees, 11 resistant genes such as BL2a_1 (penicillin), catB5 (chloramphenicol) and tetPA (tetracycline) were removed completely, and the contents of 19 resistant genes such as BL1_asba (cephalosporin), EreA (erythromycin), QnrB (fluoroquinolone) and cmL_e8 (chloramphenicol) were reduced by 85%. The composting effectively reduced the resistance genes. The research shows that the degradation of antibiotics affected the level of microbial community, and Mycobacterium and Sphingobacterium changed obviously. During the application process, resistance genes migrated horizontally. The agricultural application of the compost products needs further research and demonstration. 
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