| 基于微生物完整性指数的苏州市湿地健康评价 |
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| 引用本文： | 朱文婷,钱玮,于淼,宣雄智.基于微生物完整性指数的苏州市湿地健康评价[J].环境科学研究,2019,32(3):423-430. |
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| 作者姓名： | 朱文婷 钱玮 于淼 宣雄智 |
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| 作者单位： | 苏州农业职业技术学院,江苏 苏州,215008;苏州科技大学,江苏 苏州,215009 |
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| 基金项目： | 江苏省高等学校自然科学研究面上项目（No.17KJB610011）；苏州市科技计划项目（No.SNG2017059）；苏州农业职业技术学院青年提升计划项目（No.PPN201710） |
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| 摘 要： | 为建立在不同区域和不同湿地等大尺度范围内M-IBI（微生物生物完整性指数）评价方法体系，以苏州市15个湿地公园作为研究对象，构建符合区域性特征的生物完整性评价体系并进行应用研究.分别于2015年、2016年和2017年每年的7月对苏州市15个湿地公园的入流口和出流口水质进行采样调查，将采样点分为对照点、受损点和修复点，基于湿地水环境中微生物群落Illumina高通量测序技术，依据候选指标分布范围检验、敏感性分析和相关性检验，筛选出香农指数、前2优势分类单元相对丰度（相对丰度最多的2个属）总和、高温耐受属相对丰度、污染耐受属相对丰度、TN（总氮）敏感属相对丰度5个指标，提出了参数标准化公式和健康评价标准，依此将苏州市湿地健康状态划分为健康、亚健康、一般、较差、差5个等级.结果表明:①在10个受损点3 a所采集的30个样品中，有2个处于较差水平，有5个处于一般水平，有18个处于亚健康水平，有5个处于健康水平；而对于修复点，有23个样点处于健康水平，7个样点处于亚健康水平.②在农业面源污染较多的湿地入流口健康状况最差，工业用水汇入较多的湿地入流口次之，而城市景观水汇入的湿地入流口的健康状况最好.研究显示，M-IBI可以较合理地评价苏州市湿地生态系统健康状况，湿地对水环境的修复作用明显，经修复后的水体均处于健康或亚健康水平.
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| 关 键 词： | 微生物群落 生物完整性指数 湿地健康评价 |
| 收稿时间： | 2018/3/20 0:00:00 |
| 修稿时间： | 2018/9/17 0:00:00 |
Health Evaluation of Wetlands in Suzhou City based on Microbiome Index of Biotic Integrity |
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| ZHU Wenting,QIAN Wei,YU Miao and XUAN Xiongzhi.Health Evaluation of Wetlands in Suzhou City based on Microbiome Index of Biotic Integrity[J].Research of Environmental Sciences,2019,32(3):423-430. |
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| Authors: | ZHU Wenting QIAN Wei YU Miao and XUAN Xiongzhi |
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| Affiliation: | 1.Suzhou Polytechnic Institute of Agricultures, Suzhou 215008, China2.Suzhou University of Science and Technology, Suzhou 215009, China |
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| Abstract: | In order to solve the problems of establishing the M-IBI (microbial index of biotic integrity) evaluation system in different regions and different wetlands, 15 wetland parks in Suzhou City were used as research areas to construct a biological integrity evaluation system with regional characteristics. Water samples were collected at the inlets and outlets of 15 wetland parks in Suzhou City in July from 2015 to 2017. The sampling points were divided into damaged points and reference points. Based on the Illumina high throughput sequencing information of the microbial community in the wetlands, 5 indexes, such as the sum of relative abundances of the first 2 dominant taxa, relative abundances of high temperature tolerance genus, relative abundances of high pollution tolerance genus, relative abundances of TN sensitive genus, were selected according to the distribution range test of the candidate index, sensitivity analysis and correlation tests. The standardized formula of parameters and the standards of health evaluation were put forward, and the health status of Suzhou City wetlands was divided into 5 health grades:healthy, sub-healthy, general, poor and bad. The results showed that of the 30 samples collected at the 10 damaged points in 3 years, 2 samples were at poor level, 5 samples at general level, 18 samples at sub health level and 5 samples at health level, while based on the repair points, 23 samples samples were in health level, and 7 sites were in sub-health level. The health status of the wetland inflow with agricultural effluent were the worst, and the wetland inflow with industrial effluent were the next, and the health status of the wetland inflow with the urban effluent were the best. The research shows that the M-IBI can be used for reasonable evaluation of the health status of wetland ecosystem in Suzhou City. The restoration of the wetlands was obvious, and the restored water bodies were at the healthy or sub-health levels. |
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| Keywords: | microbial community index of biotic integrity wetland health assessment |
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