| 基于EEM与高通量技术分析中药渣投加对餐厨垃圾堆肥的影响 |
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| 引用本文： | 廖黎明,潘家琦,陈钰,胡耀元,莫辉,卢钰,宿程远.基于EEM与高通量技术分析中药渣投加对餐厨垃圾堆肥的影响[J].环境工程,2021,39(1):142-147. |
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| 作者姓名： | 廖黎明 潘家琦 陈钰 胡耀元 莫辉 卢钰 宿程远 |
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| 作者单位： | 珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541004;广西师范大学环境与资源学院,广西桂林541004;广西师范大学环境与资源学院,广西桂林541004 |
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| 基金项目： | 广西自然科学基金项目（2017GXNSFAA198277）；广西师范大学博士科研启动基金项目（2016BQ003）；广西研究生教育创新计划项目（XYCSZ2019077）。 |
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| 摘 要： | 以中药渣作为调理剂与外加碳源，利用三维荧光（EEM）考察了不同质量配比对餐厨垃圾与城市污泥共堆肥过程腐熟程度的影响，并采用高通量测序技术对不同堆体中的微生物群落进行探究。EEM分析结果表明:中药渣的添加可加快堆体腐熟进程，且随着投加量的增加，效果不断增强。而通过高通量测序可知:随着中药渣投加量的增加，堆体中微生物的生物多样性逐渐降低，但微生物群落丰富度逐渐增大；中药渣的添加会降低变形菌门的相对丰度，但会提高厚壁菌门的相对丰度；添加质量分数为5%的中药渣可提高菌体中梭菌纲的数量，从而促进堆体中纤维素的分解，且可避免中药渣添加过多所引起的堆体局部板结。总体而言，质量分数为5%中药渣的投加对于餐厨垃圾与城市污泥共堆肥过程的促进作用最佳。
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| 关 键 词： | 中药渣 餐厨垃圾 城市污泥 三维荧光 高通量测序 |
| 收稿时间： | 2020-05-16 |
ANALYSIS OF EFFECT OF ADDITION OF CHINESE HERBAL RESIDUE ON FOOD WASTE COMPOSTING BASED ON EEM AND HIGH-THROUGHPUT SEQUENCING |
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| Affiliation: | 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541004, China;2. School of Environment and Resources, Guangxi Normal University, Guilin 541004, China |
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| Abstract: | Using Chinese herbal residue as a conditioner and an external carbon source, the effect of different quality ratios on the composting process of food waste and municipal sludge were investigated by EEM spectra. In addition, the microbial communities in different piles were analyzed by using high throughput sequencing technology. The results of the EEM spectra showed that the addition of Chinese herbal residue accelerated the maturity process of composting. With the increase of the dosage, the effect was continuously enhanced. According to high throughput sequencing analysis, with the increase of the dosage of Chinese herbal residue, the microbial biodiversity in the piles gradually decreased, but the microbial community richness increased. The relative abundance of Proteobacteria decreased by adding Chinese herbal residue, while the relative abundance of Firmicutes increased. Meanwhile, adding a weight ratio 5% of Chinese herbal residue could improve the number of Clostridia, so as to promote the decomposition of cellulose in the piles, and avoid the local hardening of the piles caused by the excessive addition of Chinese herbal residue. In general, the addition of 5% Chinese herbal residue had best promoting effect on the composting process of food waste and municipal sludge. |
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