| 耐镉菌株的分离及其对Cd2+的吸附富集 |
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| 引用本文： | 刘爱民,黄为一.耐镉菌株的分离及其对Cd2+的吸附富集[J].中国环境科学,2006,26(1):91-95. |
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| 作者姓名： | 刘爱民 黄为一 |
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| 作者单位： | 1. 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏,南京,210095;安徽师范大学生命科学学院,安徽,芜湖,241000
2. 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏,南京,210095 |
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| 摘 要： | 采用梯度浓度驯化的方法,筛选分离出一株能高度耐镉和富集镉的菌株,初步鉴定为假单胞菌属(Pseudomonas sp.).该菌株在含Cd2+浓度4500mg/L的液体细菌培养基中能够生存,当Cd2+浓度为100mg/L时,pH值为6～8有利于提高菌株耐镉能力.Cd2+为低浓度时菌株积累Cd2+,菌体对Cd2+浓度为100mg/L的积累率达99.1%,冻干菌体对Cd2+的吸附量为8.786mg/g干菌体;当Cd2+浓度大于909mg/L时,菌体表现出外排Cd2+现象.红外分析和电镜观察表明,菌株细胞壁在吸附Cd2+中起重要作用,67.4%的Cd2+吸附在细胞壁上.
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| 关 键 词： | 耐镉细菌 富集 吸附 外排Cd2 红外分析 电镜观察 |
| 文章编号： | 1000-6923(2006)01-0091-05 |
| 修稿时间： | 2005年5月20日 |
Separation of tolerant cadmium bacterium strain and its accumulation adsorption of Cd2+ |
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| LIU Ai-min,HUANG Wei-yi.Separation of tolerant cadmium bacterium strain and its accumulation adsorption of Cd2+[J].China Environmental Science,2006,26(1):91-95. |
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| Authors: | LIU Ai-min HUANG Wei-yi |
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| Abstract: | A strain capable of tolerating and accumulating cadmium, was separated through concentration gradient taming technique and was identified preliminarily as Pseudomonas sp.. This strain could survive in liquid bacterium medium containing 4500mg/L of Cd2+. At100mg/L of Cd2+, the pH value of 6-8 was favorable to enhancing the strain's tolerance to Cd2+. At low concentration of Cd2+, the strain accumulated Cd2+, and the accumulation rate was 99.1%; the Cd2+ adsorption quantity was 8.786mg/g dry bacterium body. At above 909mg/L of Cd2+, the strain showed the capability of excluding Cd2+. Infrared spectroscopy analysis and electron microscopic observation indicated that the cell wall of the strain played an important role in adsorbing Cd2+, more than 67.4% Cd2+ adsorbed on the cell wall. |
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| Keywords: | tolerant-cadmium bacterium accumulation adsorption excluding Cd~(2+) infrared spectroscopy analysis electron microscopic observation |
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