| 毒死蜱暴露对蚯蚓的亚慢性神经毒性研究 |
| |
| 引用本文： | 杨易梅,周世萍,李惠娟,李奇生,武自强,杨广斌,朱鑫泽.毒死蜱暴露对蚯蚓的亚慢性神经毒性研究[J].环境科学研究,2023,36(2):414-421. |
| |
| 作者姓名： | 杨易梅 周世萍 李惠娟 李奇生 武自强 杨广斌 朱鑫泽 |
| |
| 作者单位： | 1.西南林业大学，西南地区林业生物质资源高效利用国家林业和草原局重点实验室，云南 昆明 650224 |
| |
| 基金项目： | 国家自然科学基金项目(No.31860155)；西南地区林业生物质资源高效利用国家林业和草原局重点实验室基金项目(No.2021-KF01) |
| |
| 摘 要： | 蚯蚓作为土壤生态系统中重要的生物类群，在维持土壤生态系统的功能中起着重要作用.毒死蜱是我国使用量较大的有机磷农药品种之一，对以蚯蚓为代表的土壤生物具有潜在威胁.本文选择云南林地常见的蚯蚓优势种—皮质远盲蚓(Amynthas corticis)作为试验生物，从毒死蜱暴露引发的蚯蚓脑部神经递质(乙酰胆碱、多巴胺、5-羟色胺、谷氨酸)、离子通道特征酶(Ca2+-Mg2+-ATP酶、Ca2+-ATP酶)变化入手，对毒死蜱亚慢性神经毒性导致的蚯蚓运动、学习记忆等神经行为变化进行研究，运用综合毒性指数(IBR)评价蚯蚓生物标志物变化，开展毒死蜱暴露对蚯蚓的亚慢性神经毒性研究.结果表明：暴露期内，毒死蜱处理组中蚯蚓脑部的乙酰胆碱含量显著高于对照组(P<0.05)，且随着暴露时间的增加，毒死蜱处理组中蚯蚓脑部乙酰胆碱含量持续升高，而蚯蚓脑部多巴胺、5-羟色胺、谷氨酸含量则均显著低于对照组(P均小于0.05).随着暴露时间的增加，毒死蜱对这3种神经递质含量的抑制加强，毒死蜱对蚯蚓的神经损伤导致了蚯蚓的运动迟缓和学习记忆障碍，毒死蜱暴...
|
| 关 键 词： | 蚯蚓 毒死蜱 亚慢性神经毒性 神经行为 土壤污染 |
| 收稿时间： | 2022-06-09 |
Subchronic Neurotoxicity of Chlorpyrifos Exposure to Earthworms |
| |
| Affiliation: | 1.Key Laboratory of State Forestry Administration for Highly-Efficient Utilization of Forestry Biomass, Southwest Forestry University, Kunming 650224, China2.Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China |
| |
| Abstract: | As an important biological species in soil ecosystems, earthworms play a significant role in maintaining the function of soil ecosystems. Chlorpyrifos is one of the most widely used organophosphorus pesticides in China, which poses potential threat to soil organisms represented by earthworms. In this study, we selected Amynthas corticis, a dominant earthworm species commonly found in Yunnan woodlands, as the experimental organism. The changes in the neurotransmitters (acetylcholine, dopamine, 5-hydroxytryptamine, glutamate) and ion channel characteristic enzymes (Ca2+-Mg2+-ATPase, Ca2+-ATPase) in the brain of earthworms induced by chlorpyrifos exposure were studied. The subchronic neurotoxicity of chlorpyrifos on earthworms was investigated using integrated biomarker response IBR. The results showed that the acetylcholine content in the brain of earthworms in the chlorpyrifos-treated group was significantly higher than that in the control group during the exposure period (P<0.05), and the acetylcholine content continued to increase with the increase of exposure time, while the dopamine, 5-hydroxytryptamine and glutamate contents in the brain of earthworms were significantly lower than those in the control group (P<0.05). With increasing exposure time, the inhibitory effect of chlorpyrifos on the content of these three neurotransmitters increased, and the neurological damage caused by chlorpyrifos to earthworms led to movement retardation and learning-memory impairment in earthworms. Chlorpyrifos exposure-induced neurotransmitter abnormalities in earthworms are one of the possible mechanisms of neurobehavioral changes in movement retardation and learning-memory impairment in earthworms. The subchronic neurotoxicity IBR values of 8 and 12 mg/kg chlorpyrifos for earthworms at 28 d exposure were 3.2275 and 4.5246, respectively. The IBR values at 56 d exposure were 3.8848 and 5.8995, respectively, and the subchronic neurotoxicity of chlorpyrifos to earthworms increased with increasing exposure concentration and time. It was found that chronic neurotoxicity of chlorpyrifos did not leading to the death of earthworms, but caused neurobehavioral changes such as movement retardation and memory impairment in earthworms. |
| |
| Keywords: | |
|
| 点击此处可从《环境科学研究》浏览原始摘要信息 |
|
点击此处可从《环境科学研究》下载全文 |
|
