| 快速检测痕量铜的光电型传感器的研制 |
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| 引用本文： | 商璟,柴春彦,翁芝莹,张洪才,刘国艳.快速检测痕量铜的光电型传感器的研制[J].环境监测管理与技术,2012,24(2):45-47. |
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| 作者姓名： | 商璟 柴春彦 翁芝莹 张洪才 刘国艳 |
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| 作者单位： | 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 上海市科委技术标准专项基金资助项目（06DZ05036） |
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| 摘 要： | 利用铜对葡萄糖氧化酶的抑制作用,以及葡萄糖氧化酶-辣根过氧化物酶-葡萄糖(Glu)-邻联甲苯胺(OT)偶联反应体系生成蓝色底物的原理,将Glu与OT固定化制成试纸条,插入自制的便携式单色光反射计,组装出检测铜的光电型传感器。当铜的质量浓度范围为1 mg/L～100 mg/L时,光反射强度值与铜的质量浓度呈良好的线性关系。在优化的试验条件下,该方法的检测限为0.064 mg/L,检测时间为5 min。
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| 关 键 词： | 铜 光电型传感器 葡萄糖氧化酶-辣根过氧化物酶-葡萄糖-邻联甲苯胺 快速检测 |
Research of Photoelectric Sensor for Fast Test of Trace Copper |
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| SHANG Jing
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CHAI Chun-yan
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WENG Zhi-ying
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ZHANG Hong-cai
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LIU Guo-Yan.Research of Photoelectric Sensor for Fast Test of Trace Copper[J].The Administration and Technique of Environmental Monitoring,2012,24(2):45-47. |
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| Authors: | SHANG Jing CHAI Chun-yan WENG Zhi-ying ZHANG Hong-cai LIU Guo-Yan |
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| Affiliation: | (College of Biology and Agriculture,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | A photoelectric sensor was composed by home-made portable monochromatic light reflector which inserted a test strip with immobilized glucose and o-tolidine for copper detection.Principle of the photoelectric sensor was that inhibitory effect of copper on glucose oxidase formed blue substrate by using a coupling reaction system of glucose oxidase-horseradish peroxidase-glucose-o-tolidine.There was good correlative linearity between 1 mg/L~100 mg/L copper solution and light reflecting intensity.The detection limit of method was 0.064 mg/L,testing time 5 minutes after optimization of the experimental condition. |
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| Keywords: | Copper Photoelectric sensor Glucose oxidase-horseradish-glucose（Glu）-o-tolidine（OT） Fast test |
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