| 消化污泥序批式驯化中产气性能和微生物多样性变化 |
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| 引用本文： | 刘新媛,胡文甲,欧阳帆,聂家民,吴楠,杨帆,孔丝纺.消化污泥序批式驯化中产气性能和微生物多样性变化[J].环境工程,2021,39(3):136. |
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| 作者姓名： | 刘新媛 胡文甲 欧阳帆 聂家民 吴楠 杨帆 孔丝纺 |
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| 作者单位： | 1. 天津农学院 工程技术学院, 天津 300384; |
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| 基金项目： | 天津市应用基础与前沿技术研究计划项目（16JCQNJCO8200）；天津农学院科研发展基金计划（20190115）；广东高校青年创新人才类项目（2017GkQNCX066）；深圳市科技计划（JCYJ20180307155011964）。 |
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| 摘 要： | 利用序批式运行方法探索低活性厌氧接种污泥对新底物的适应过程。研究发现，接种污泥经过1个批次驯化后，后续批次的甲烷产量维持稳定，说明首批次培养实现了有效的代谢调整。分析各发酵批次的pH值和产甲烷动力学参数发现，随着驯化批次的增加，接种污泥产甲烷的延迟期缩短、产甲烷速率升高，且各批次初期酸化程度降低，说明了多批次驯化促进污泥对底物的适应能力。高通量测序技术分析驯化前后微生物群落结构可知，3个批次驯化后污泥菌群的丰富度和多样性均降低。驯化筛选出以Paludibacter属（相对丰度为52.3%）和Methanosaeta属（相对丰度为72.31%）为优势菌属的群落结构。
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| 关 键 词： | 消化污泥 产甲烷 驯化培养 厌氧发酵 菌群结构 |
| 收稿时间： | 2020-02-27 |
BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE |
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| Affiliation: | 1. College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300384, China;2. Traffic and Environment Department, Shenzhen Institute & Information Technology, Shenzhen 518172, China |
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| Abstract: | This article investigated the adaptability changes of slight-activated seed sludge to new substrate during anaerobic sequencing batch acclimatization. Results showed that the seed sludge had stable methane production ability in the second and third batch after the acclimatization in the first batch, indicating that the effective metabolic adjustment had been obtained in the first batch. According to the results of the pH value and kinetic parameters for methane production in each batch, it was found the delay period for methane production was shortened, the methane production rate was increased and the acidification degree in the initial period of each batch was reduced with the acclimated batch. The results indicated that the adaptability of seed sludge to substrate was gradually enhanced. The microbial community structure before and after sequencing batch culture were detected by high throughput sequencing technology. Results showed that the richness and diversity of acclimated sludge reduced after the three times batch culture. The dominant bacterial genus of Paludibacter with relative abundance of 52.3% and the dominant archaeal genus of Methanosaeta with relative abundance of 72.31%, were selected during the acclimatization. |
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