| 3次投加H2O2预氧化对石油污染土壤中石油烃生物降解的研究 |
| |
| 引用本文： | 徐金兰,杨正利.3次投加H2O2预氧化对石油污染土壤中石油烃生物降解的研究[J].环境工程,2023,41(2):122-130. |
| |
| 作者姓名： | 徐金兰 杨正利 |
| |
| 作者单位： | 1. 西安建筑科技大学 环境与市政工程学院, 西安 710055; |
| |
| 基金项目： | 国家自然科学基金项目(51778524) |
| |
| 摘 要： | 为得到一种高效降解石油烃(TPH)的Fenton预氧化联合生物修复石油污染土壤的修复方式，考察了分3次投加不同浓度H2O2预氧化后羟基自由基(·OH)特征、NH+4-N浓度、DOC浓度、脱氢酶及多酚氧化酶活性，以及石油烃去除量。结果表明：5 g石油污染土样体系中，将900 mmol/L的H2O2(4.635 mL)分3次投加预氧化后，所产生的·OH最大瞬时强度低，·OH的持续时间短，对石油降解菌杀伤作用少，TPH氧化量较高。生物修复前20 d, NH+4-N浓度消耗量较高(170.45 mg/kg),石油降解菌菌量增长快，长链烷烃C21—C30(22%)和DOC降解率(69%)高，生物修复前期充足NH+4-N浓度的消耗诱导石油降解菌在0～50 d高效降解长链烷烃(42%)。脱氢酶活性和多酚氧化酶活性在20 d达到顶峰，表明微生...
|
| 关 键 词： | Fenton预氧化 NH+4-N 长链烷烃 生物修复 酶活性 |
| 收稿时间： | 2022-03-06 |
IMPACTS OF PETROLEUM HYDROCARBONS BIODEGRADATION IN OIL-CONTAMINATED SOIL AFTER PRE-OXIDATION WITH THREE BATCHS H2O2 ADDITION |
| |
| Affiliation: | 1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;2. Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China |
| |
| Abstract: | To obtain a remediation method of Fenton pre-oxidation combined with bioremediation of oil-contaminated soil for efficient degradation of petroleum hydrocarbons (TPH), the characteristics of hydroxyl radical (·OH), NH+4-N concentration, DOC concentration, dehydrogenase and polyphenol oxidase activities and TPH removal with dosing different concentration of H2O2 by three batches were investigated in this study. The results showed that the maximum instantaneous intensity and duration of ·OH were low, the bacteria destruction was low and TPH oxidation was high after pre-oxidation with 900 mmol/L H2O2 (4.635 mL) by three batches dosing. The NH+4-N consumption (170.45 mg/kg) was high and the quantity of hydrocarbon-degrading microorganisms increased rapidly, then long-chain alkanes C21 to C30 (22%) and DOC (69%) degradation were high at the first 20 days in bio-remediation. Therefore, hydrocarbon degraders were induced to degrade long-alkane (42%) in 0 to 50 days by sufficient NH+4-N concentration consumption in the early bio-remediation stage. The activities of dehydrogenase and polyphenol oxidase reached a peak on the 20th day, indicating that the metabolic activity of microorganisms increased, and petroleum hydrocarbon degradation mainly occurred in this period. |
| |
| Keywords: | |
|
| 点击此处可从《环境工程》浏览原始摘要信息 |
|
点击此处可从《环境工程》下载全文 |
|
