| 可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性 |
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| 引用本文： | 孙磊,宋彤彤,朱珍妮,李丹阳,刘存歧.可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性[J].环境科学研究,2016,29(12):1882-1886. |
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| 作者姓名： | 孙磊 宋彤彤 朱珍妮 李丹阳 刘存歧 |
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| 作者单位： | 1.河北大学生命科学学院, 河北 保定 071002 ;河北省微生物多样性研究与应用实验室, 河北 保定 071002 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项(2012ZX07203003)；河北省生物工程重点学科项目 |
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| 摘 要： | 为治理制药废水中残留的难降解有机污染物TEA(triethylamine,三乙胺),以石家庄某污水处理厂的活性污泥为材料,采用富集培养和选择培养,分离筛选到1株能以TEA为唯一碳源和氮源生长代谢的降解菌——S6-2.通过测定形态特征、生理生化特性、G+C(碱基对)摩尔百分比及16S rRNA基因序列系统发育分析,该菌株被鉴定为赤红球菌(Rhodococcus ruber).采用单一影响因素试验分析菌株S6-2对TEA的降解特性,结果表明:菌株S6-2具有较强的TEA降解能力及降解稳定性；其最适降解温度为32 ℃,最适降解pH为8.0.菌株S6-2可耐受较高浓度TEA,在ρ(TEA)为900 mg/L的培养基中仍能生长；在最适条件下,菌株S6-2对TEA的降解率为70.7%±1.8%.该株菌在含TEA的无机盐平板上传代培养15代后,对TEA的降解率为69.3%±2.5%,说明菌株S6-2对TEA的降解具有稳定性.研究显示,菌株S6-2可作为TEA污染水体生物修复的潜在资源.
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| 关 键 词： | 三乙胺 生物降解 赤红球菌 分离鉴定 |
| 收稿时间： | 2016/3/21 0:00:00 |
| 修稿时间： | 2016/6/21 0:00:00 |
Screening,Identification and Characterization of a Triethylamine Degrading Bacterium Rhodococcus ruber S6-2 |
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| Affiliation: | 1.College of Life Sciences, Hebei University, Baoding 071002, China ;Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, China2.College of Life Sciences, Hebei University, Baoding 071002, China |
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| Abstract: | In order to treat wastewater contaminated by triethylamine, which is generated from pharmaceutical factories, bacterial strains which could degrade triethylamine were screened. A bacterial strain S6-2 that used triethylamine as the sole carbon and energy source was isolated from the activated sludge of a wastewater treatment plant in Shijiazhuang by enrichment and selective medium. S6-2 was identified as Rhodococcus ruber by morphological, physiological and biochemical characteristics, as well as (G+C)mol percentage and 16S rRNA gene phylogenic analysis. Through single-factor experiments of degrading features, S6-2 was shown to have high degradation ability and stability. The study demonstrated that temperature and pH were important factors for triethylamine degradation. The optimal temperature was 32 ℃, and the optimal pH was 8.0.This strain was tolerant to a high concentration oftriethylamine and grew normally in tradethylamine as high as 900 mg/L. Under the optimal conditions, the triethylamine degradation rate reached 70.7%±1.8%. After 15 generations subcultured on mineral salt plates supplemented with triethylamine, the triethylamine degradation rate was 69.3%±2.5%. The results showed that S6-2 had steady ability of triethylamine degradation, and suggested that S6-2 could be a potential strain for treating triethylamine-contaminated wastewater. |
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