| 甲硫醇脱臭菌的分离、分子鉴定及应用 |
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| 引用本文： | 刘 波,闫懂懂,毕雪梅.甲硫醇脱臭菌的分离、分子鉴定及应用[J].环境科学研究,2007,20(1):104-107. |
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| 作者姓名： | 刘 波 闫懂懂 毕雪梅 |
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| 作者单位： | 1.南京大学 污染控制与资源化国家重点实验室,江苏 南京 210093 |
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| 基金项目： | 国家自然科学基金
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南京大学校科研和教改项目 |
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| 摘 要： | 以低浓度甲硫醇臭气的生物降解为研究对象， 从不同的菌源中筛选得到一株能高效降解甲硫醇的菌株.运用16S rDNA的分子鉴定技术，确定筛选菌Jll的16S rDNA序列同芸苔根际菌(Rape rhizosphere)的相似性最大(97%)，为同属.筛选菌Jll生长迅速，挂膜时间短.将其接种于生物滴滤反应器中，在甲硫醇进气流量为0.3 m3/h，质量浓度为40 mg/m3，运行仅4 d后，其去除率可逐渐升到100%.当进气流量分别为0.3，0.6和1.2 m3/h时，该反应器对甲硫醇的最大去除能力分别为36.2，41.8和16.4 mg/(m3·h).反应器进气负荷提高到(96±3) mg/(m3·h)，运行一段时间后，恢复适宜的进气负荷，生物滴滤反应器对甲硫醇的去除率很快回升.这表明菌Jll不但降解能力高，而且抗逆性强.
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| 关 键 词： | 16S rDNA基因 甲硫醇 芸苔根际菌(Rape rhizosphere) 生物滴滤反应器 |
| 文章编号： | 1001-6929（2007）01-0104-04 |
| 修稿时间： | 2006-05-16 |
Isolation, Application and Molecular Identification of Deodorizing Bacteria for Methyl Mercaptan |
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| LIU Bo,YAN Dong-dong and BI Xue-mei.Isolation, Application and Molecular Identification of Deodorizing Bacteria for Methyl Mercaptan[J].Research of Environmental Sciences,2007,20(1):104-107. |
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| Authors: | LIU Bo YAN Dong-dong and BI Xue-mei |
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| Affiliation: | 1.National Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093, China2.The Department of Urban and Resources Sciences, Nanjing University, Nanjing 210093, China |
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| Abstract: | The objective of this research is to degrade low concentration Methyl mercaptan by microorganism. Strain Jll screened out from different odor origins could efficiently remove methyl mercaptan (MM). A systematic identification method, 16S rDNA gene sequence comparison, for deodorizing bacteria was carried out.The 16S rDNA gene sequence analysis of strain Jll showed the highest level of 97% homology to Rape rhizosphere. The strain Jll's growth was rapid and the time of deodorizing domestication shortened. After Jll was inoculated into trickled-bed biofilters, the removal efficiency was up to 100% after only 4 days, operating at the air flow rate of 0.3 m3/h and inlet pollutant concentration of 40 mg/m3. When the superficial air flow rate was 0.3, 0.6 and 1.2 m3/h,maximum eliminationcapacity of 36.2, 41.8 and 16.4 mg/(m3·h) was obtained respectively. Adjusting inlet loads of (96±3) mg/(m3·h) to suitable low inlet loads, the removal efficiency of bioreactor could improve rapidly. It shows that the Jll has powerful capacity to degrade MM and resistance to shocks. |
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| Keywords: | 16S rDNA gene methyl mercaptan Rape rhizosphere trickled-bed biofilter |
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