| 处理秸秆纤维素乙醇废水的东北土著白腐真菌筛选及特性研究 |
| |
| 引用本文： | 刘丽荣,梁红,高大文.处理秸秆纤维素乙醇废水的东北土著白腐真菌筛选及特性研究[J].环境科学研究,2018,31(7):1310-1315. |
| |
| 作者姓名： | 刘丽荣 梁红 高大文 |
| |
| 作者单位： | 1.东北林业大学林学院, 黑龙江 哈尔滨 150040 |
| |
| 基金项目： | 哈尔滨市应用技术研究与开发项目（No.2016RAXXJ010） |
| |
| 摘 要： | 为了提高秸秆纤维素乙醇废水的处理效果，选择6种东北土著白腐真菌，对2%的秸秆纤维素乙醇废水中木质素进行降解处理.采用正交试验法对筛选出的高效降解菌进行产漆酶培养基的优化.结果表明:6种白腐真菌最高酶活大小顺序为青顶拟多孔菌>血红密孔菌>糙皮侧耳菌>彩绒革盖菌>烟色烟管菌>灵芝；血红密孔菌、糙皮侧耳菌、彩绒革盖菌、青顶拟多孔菌、灵芝、烟色烟管菌在第0天起始质量浓度为640.9~716.6 mg/L，在第14天木质素的质量浓度分别为434.0、411.2、441.8、441.7、533.3、503.5 mg/L，对木质素的去除率分别为37.1%、37.0%、31.8%、31.7%、25.6%、21.4%，并分别在第12、12、4、4、2、6天木质素降解趋于平稳，表明降解效果最好的菌种为血红密孔菌.血红密孔菌产漆酶培养基最优组合方案:最佳碳源为锯末，质量浓度为35 g/L；最佳氮源为蛋白胨，质量浓度为4 g/L；最佳pH为5.极差分析表明，各因素对血红密孔菌产漆酶的影响顺序为碳源>氮源> pH >氮源质量浓度>碳源质量浓度.在最佳培养基条件下，废水中木质素降解率达41.1%.研究显示，血红密孔菌可以作为生物法处理秸秆纤维素乙醇废水的菌种资源，也可为今后的进一步应用研究提供科学依据.
|
| 关 键 词： | 白腐真菌 漆酶 木质素 秸秆纤维素乙醇废水 |
| 收稿时间： | 2017/12/1 0:00:00 |
| 修稿时间： | 2018/2/10 0:00:00 |
Screening and Characteristics of Dongbei Local White Rot Fungi Which Dispose Cellulosic Ethanol Waste Water of Straw |
| |
| LIU Lirong,LIANG Hong and GAO Dawen.Screening and Characteristics of Dongbei Local White Rot Fungi Which Dispose Cellulosic Ethanol Waste Water of Straw[J].Research of Environmental Sciences,2018,31(7):1310-1315. |
| |
| Authors: | LIU Lirong LIANG Hong and GAO Dawen |
| |
| Affiliation: | 1.School of Forestry, Northeast Forestry University, Harbin 150040, China2.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
| |
| Abstract: | In order to improve the effect of disposal of cellulosic ethanol waste water of straw, the project selected six local white rot fungi in the northeast of China to dispose by degradation the lignin in 2% cellulosic ethanol waste water of straw. The experiment adopted orthogonal test to optimize the screened efficient degradation fungi in producing laccase culture medium. The results showed that the six species of white rot fungi are Polyporellus picipes > Pycnoporus sanguineus > Pleurotus ostreatus > Coriolus uersicolor > Bjerkandera fumosa > Ganoderma lucidum in the sequence of enzyme activity; the initial concentration of P. sanguineus, P. ostreatus, C. uersicolor, P. picipes, G. lucidum and B. fumosa at the 0th day were 640.9-716.6 mg/L, the concentration of lignin at the 14th day were 434.0, 411.2, 441.8, 441.7, 533.3 and 503.5 mg/L respectively, and the removal rate for lignin were 37.1%, 37.0%, 31.8%, 31.7%, 25.6% and 21.4% respectively. The degradation trend of lignin was to be stable at the 12th, 12th, 4th, 4th, 2nd, 6th day respectively. It can be seen that the white rot fungi with the best degradation effect is P. sanguineus; P. sanguineus optimal combination of produces laccase culture medium:the best origin of carbon is saw powder with mass concentration of 35 g/L, the best nitrogen source is peptone with mass concentration of 4 g/L, the best pH is 5. the range analysis showed that the influence sequence of various factors on P. sanguineus in producing laccase is source of carbon > nitrogen source > pH > concentration of nitrogen source > concentration of carbon source; the degradation rate of lignin in waste water under the condition of the best culture medium was up to 41.1%. The research shows that P. sanguineus can be used as source of fungi for disposal of cellulosic ethanol waste water of straw by means of bioanalysis, and provides scientific basis for further application and research in the future. |
| |
| Keywords: | white rot fungi laccase lignin straw cellulose ethanol wastewater |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《环境科学研究》浏览原始摘要信息 |
|
点击此处可从《环境科学研究》下载全文 |
|
