| 混合脱氮微生物菌群的高密度培养 |
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| 引用本文： | 耿金菊,刘登如,华兆哲,陈 坚.混合脱氮微生物菌群的高密度培养[J].环境科学研究,2002,15(5):22-24. |
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| 作者姓名： | 耿金菊 刘登如 华兆哲 陈 坚 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 基金项目： | 教育部跨世纪人才培养计划基金项目 |
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| 摘 要： | 利用微生物的混合培养技术,研究了好氧条件下同时硝化-反硝化的生物脱氮过程.混合脱氮微生物菌群生长的适宜pH范围为7～10,在5 L发酵罐上探索了实现混合脱氮微生物菌群高密度培养的pH控制策略:发酵前期补酸控制pH≤8,发酵中后期不控制pH值,可缩短菌体的生长周期,提高菌体的氨氮降解速率,细胞质量浓度达3.9 g/L,比自然pH条件下提高了62.5%.并在10 L发酵罐上作进一步的培养,验证了其pH控制策略的可行性.通过分批补加(NH2)2SO4使菌浓进一步提高了15.4%,最终细胞干重为4.5 g/L.
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| 关 键 词： | 脱氮微生物菌群 高密度培养 好氧脱氧 |
| 文章编号： | 1001-6929(2002)05-0022-03 |
| 收稿时间： | 2001/11/12 0:00:00 |
High-Density Culture of Mixed Denitrifying Bacteria |
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| GENG Jin-ju,LIU Deng-ru,HUA Zhao-zhe and CHEN Jian.High-Density Culture of Mixed Denitrifying Bacteria[J].Research of Environmental Sciences,2002,15(5):22-24. |
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| Authors: | GENG Jin-ju LIU Deng-ru HUA Zhao-zhe and CHEN Jian |
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| Affiliation: | Key Laboratory of Industrial Biotechnology of Ministry of Education,Southern Yangtze University,Wuxi 214036,China |
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| Abstract: | The simultaneous aerobic nitrifying-denitrifying process was investigated using the mixed culture method.The pH value of 7～10 was the favorable condition for the growth of mixed denitrifying bacteria.To obtain high cell concentration during the cultivation using 5 L tank,pH was controlled below 8 at the initial fermentation stage,and uncontrolled afterwards.The dry cell weight reached 3 9 g/L,increased by 62 5% compared to the value in the natural pH culture conditions.Further scale-up culture was investigated in 10 L fermentation tank.The biomass was further increased by 15 4% by the feeding of ammonium sulfate,and the maximum biomass was 4 5 g/L. |
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| Keywords: | denitrifying bacteria high\|density culture aerobic denitrifying |
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