| 浮床水芹不同生长阶段水体活性物质代谢组学分析 |
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| 引用本文： | 孙林鹤,常雅军,韩士群,崔键,刘晓静,姚东瑞.浮床水芹不同生长阶段水体活性物质代谢组学分析[J].环境科学研究,2021,34(8):1860-1875. |
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| 作者姓名： | 孙林鹤 常雅军 韩士群 崔键 刘晓静 姚东瑞 |
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| 作者单位： | 江苏省中国科学院植物研究所(南京中山植物园),江苏南京 210014;江苏省水生植物资源与水环境修复工程研究中心,江苏南京 210014;江苏省中国科学院植物研究所(南京中山植物园),江苏南京 210014;江苏省农业科学院农业资源与环境研究所,江苏南京 210014 |
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| 基金项目： | 江苏省农业科技自主创新项目CX(18)2027国家自然科学基金项目32000270江苏省“六大人才高峰”高层次人才项目TD-JNHB-008 |
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| 摘 要： | 为解析浮床水芹在不同生长阶段种植水中活性代谢物特征与差异，揭示水芹化感作用与环境微生物互作机制，对水芹种植水中代谢物进行定性定量研究十分必要.该研究利用非靶向代谢组学技术，采用LC-ESI-MS分析方法对水芹繁殖期、幼苗期、成熟期3阶段种植水中代谢物进行检测，结合多元统计和单变量统计方法筛选出主要代谢物和显著差异代谢物，并对环境修复相关代谢物进行了相关性分析.结果表明:①在不同生长阶段水芹种植水中共检测到306种代谢物，13种代谢物在各时期种植水中的相对含量均超过1%，其中包括甜菜碱、硬脂酸和芥酸酰胺等参与植物抗冻、化感作用和反硝化细菌富集过程的重要代谢物.②共112种代谢物在不同时期种植水中存在显著差异，化感作用代谢物硬脂酸和植物-根际微生物互作代谢物D-（-）-奎宁酸在各时期间均为显著差异代谢物.③相关性分析发现，硬脂酸、壬二酸、肉豆蔻酸、儿茶酚、3，5-二羟基苯甲酸、阿魏酸、水杨酸、咖啡酸和苯甲酸9种化感物质含量之间具有高度相关性（r>0.6，P < 0.01），且在水芹幼苗期相对含量最高.研究显示，水芹种植水中存在至少23种环境修复相关代谢物，其中9种化感物质可能存在协同作用，幼苗期水芹更适于水芹提取物抑藻剂开发.
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| 关 键 词： | 水芹 代谢组学 根际效应 环境修复 根际微生物 化感作用 |
| 收稿时间： | 2020-12-26 |
Metabolomics Analysis of Bioactive Compounds in Culture Water Planting Floating Bed Water Dropwort in Different Development Stages |
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| Affiliation: | 1.Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China2.Jiangsu Engineering Research Center of Aquatic Plant Resources and Water Environment Remediation, Nanjing 210014, China3.Agriculture Resource and Environment Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | In order to investigate the characteristics and differences of culture water planting floating bed water dropwort and reveal the mechanism of allelopathy and the crosstalk with microorganisms, it is necessary to study the metabolites in the culture water. The content of metabolites in the culture water planting the water dropwort of three different stages (proliferation stage, juvenile stage and adult stage) was quantified via LC-ESI-MS based non-targeted metabolomics technology. The primary metabolites and the metabolites with significant difference were identified through multivariate statistics and univariate statistics. The correlation analysis between important metabolites and environmental remediation was carried out. The results showed that: (1) 306 metabolites were detected in the culture water at different stages. The content ratios of 13 metabolites at each stage were above 1%. Among them, compounds like stearic acid, betaine and erucamide were the key metabolites for cold tolerance, allelopathy and denitrifying bacteria accumulation. (2) 112 metabolites were significantly different metabolites between stages, allelopathy metabolite stearic acid and plant-microbe crosstalk metabolite D-(-)-quinic acid were significantly different metabolites between any stages. (3) Correlation analysis results showed that the contents of 9 allelopathy metabolites, azelaic acid, myristic acid, catechol, 3, 5-dihydroxybenzoic acid, ferulic acid, salicylic acid, stearic acid, caffeic acid and benzoic acid were highly corelated (r > 0.6, P < 0.01) and the content was the highest in the juvenile stage. Taken together, at least 23 metabolites in the culture water are related to environmental remediation, 9 of them may work together during the allelopathy of water dropwort and water dropwort in the juvenile stage is more suitable for the developing of water dropwort algae inhibitor. |
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