| 模拟光伏曝气SBR除磷性能及其聚磷菌群落结构 |
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| 引用本文： | 高兴华,周北海,徐九华,马方曙.模拟光伏曝气SBR除磷性能及其聚磷菌群落结构[J].环境科学研究,2015,28(12):1908-1916. |
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| 作者姓名： | 高兴华 周北海 徐九华 马方曙 |
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| 作者单位： | 北京科技大学土木与环境工程学院, 北京 100083 |
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| 基金项目： | 国家海洋公益性行业科研专项(201405035) |
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| 摘 要： | 基于村落生活污水排水、日光昼夜变化和SBR(序批式反应器)运行方式的特点及强化生物除磷所需的交替厌氧/好氧环境,采用模拟光伏曝气SBR处理生活污水,考察反应器的除磷效果及机理,并采用PCR(聚合酶链式反应)和TA克隆考察反应器内PAO(聚磷菌)的群落结构. 结果表明,反应器在12 d内成功启动,TP去除率从35.4%逐渐升至98.8%,此后TP去除率一直稳定在97%以上,出水ρ(TP)小于0.1 mg/L. 在反应段末期,反应器污泥中w(TP)达60 mg/g以上,其中w(complex-P)(complex-P为胞内聚合磷)达到55.0 mg/g,而w(EPS-P)(EPS-P为胞外聚合物结合磷)所占比例<10%,表明EPS吸附对除磷的贡献很小,除磷主要通过PAO的释磷/吸磷过程实现. 所有PAO均属于“Candidatus Accumulibacter”世系,Ⅱ型Candidatus Accumulibacter(包括Clade ⅡA-Clade ⅡD)是反应器内主要的PAO,其在3个克隆文库(d30、d60、d90)中所占比例分别为80.0%、80.0%和76.7%.
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| 关 键 词： | 光伏曝气 强化生物除磷 SBR 聚磷菌 |
| 收稿时间： | 2015/6/15 0:00:00 |
| 修稿时间： | 2015/7/28 0:00:00 |
Phosphorous Removal Performance and Polyphosphate-Accumulating Organism Community Structure in a Simulated PV Aeration SBR |
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| GAO Xinghu,ZHOU Beihai,XU Jiuhua and MA Fangshu.Phosphorous Removal Performance and Polyphosphate-Accumulating Organism Community Structure in a Simulated PV Aeration SBR[J].Research of Environmental Sciences,2015,28(12):1908-1916. |
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| Authors: | GAO Xinghu ZHOU Beihai XU Jiuhua and MA Fangshu |
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| Affiliation: | School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China |
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| Abstract: | A simulated PV aeration system without batteries was proposed for domestic wastewater treatment, considering the features of rural sewage discharge, solar diurnal variation, the operation mode of SBR and the required anaerobic/aerobic conditions for enhanced biological phosphorus removal (EBPR). The phosphorous removal performance and mechanism were investigated. Moreover, the polyphosphate-accumulating organism (PAO) community structure was evaluated based on PCR and TA cloning. The results showed that the novel reactor was started-up successfully within 12 days, and the phosphorous removal rate increased from 35.4% to 98.8%. Thereafter, the mean TP removal rate was maintained stably above 97%, and the mean TP concentration in the effluent was below 0.1 mg/L. At the end of the reaction stage, the phosphorous contends of the active sludge were above 60 mg/g. The biologically stored phosphorus (complex P) reached as high as 55.0 mg/g, and was the major fraction of phosphorus in the sludge. The EPS adsorbed phosphorus accounted for less than 10% of the removed phosphorus, indicating the phosphorous removal was mainly carried out by the phosphorous release/uptake process of PAO. Adsorption of EPS played an unimportant role in the phosphorus removal process. All the PAO belonged to “Candidatus Accumulibacter” lineage; type Ⅱ “Candidatus Accumulibacter,” including Clade Ⅱ A-Clade Ⅱ D, was the most enriched PAO in the reactor, containing 80.0%, 80.0% and 76.7% of the PAO ppk1 sequences in clone libraries of d30, d60 and d90. |
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| Keywords: | PV aeration enhanced biological phosphorous removal SBR polyphosphate-accumulating organism |
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