首页 | 官方网站   微博 | 高级检索  
     

硫氰酸钠对斑马鱼毒性的评价
引用本文:廖静,谷浩,牟文,张灿,熊丽.硫氰酸钠对斑马鱼毒性的评价[J].生态毒理学报,2011,6(1):43-47.
作者姓名:廖静  谷浩  牟文  张灿  熊丽
作者单位:华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉 430079;华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉 430080;华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉 430081;华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉 430082;华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉 430083
基金项目:全国大学生创新性实验;国家自然科学基金(No. 30771429; No. 20672041)
摘    要:为了研究植物性饲料代谢产生的中间产物硫氰酸钠(NaSCN)对水生生物的毒性效应,首先将斑马鱼(Brachy danio rerio)暴露于NaSCN的8个浓度组中进行96 h-LC50急性毒性试验,测得其半致死浓度(96 h-LC50)为403 mg·L-1.再以安全浓度(1/10LC50.)为基准,等对数设置3个质量...

关 键 词:硫氰酸钠(NaSCN)  斑马鱼  Na+/K+-ATPase  乙酰胆碱酯酶(ACHE)  MDA
收稿时间:2009/12/24 0:00:00
修稿时间:2010/3/30 0:00:00

Assessment of NaSCN Toxicity on Brachydanio rerio
Liao Jing,Gu Hao,Mu Wen,Zhang Can and Xiong Li.Assessment of NaSCN Toxicity on Brachydanio rerio[J].Asian Journal of Ecotoxicology,2011,6(1):43-47.
Authors:Liao Jing  Gu Hao  Mu Wen  Zhang Can and Xiong Li
Abstract:To study the toxic effects of NaSCN on the aquatic specials,Brachydanio rerio were exposed to NaSCN of eight different concentrations for 96 h-LC50 measurement.It shows that the 96 h-LC50 is 403 mg·L-1,thus the safe concentration(1/10 LC50) is 40.3 mg·L-1.Next,the safe concentration was set as the standard,three NaSCN concentrations(22.7,40.3,71.7 mg·L-1) were confected using the same logarithmic distance with a control group(0 mg·L-1),and 96 hours' acute toxicity test was carried out.In the meantime,the activities of AChE and Na+/K+-ATPase in B.rerio brain tissue were measured;the MDA contents in B.rerio body tissue were also measured.The results of ANOVA test show that the AChE activities of NaSCN exposure groups were not significantly different compared with the control group.While the Na+/K+-ATPase activities and the MDA contents of 71.7 mg·L-1 group were significantly different(p =0.01) compared with the control group.The contents of MDA and the concentrations of NaSCN showed obvious dose-response relation.Thus,it was deduced that the toxicity mechanism of NaSCN to B.rerio was mainly resulting from the dysfunction of mitochondrion.And MDA content could be used as an effective biomarker for toxicity assessment of NaSCN to B.rerio.
Keywords:NaSCN  Brachydanio rerio  AChE  Na+/K+\|ATPase  MDA
本文献已被 万方数据 等数据库收录!
点击此处可从《生态毒理学报》浏览原始摘要信息
点击此处可从《生态毒理学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号