Effects of pCO2 on the interaction between an excavating sponge,Cliona varians,and a hermatypic coral,Porites furcata

Rising dissolved pCO₂ is a mounting threat to coral reef ecosystems. While the biological and physiological impacts of increased pCO₂ are well documented for many hermatypic corals, the potential effects on bioerosion processes remain largely unknown. Increases in pCO₂ are likely to modify the direct interactions between corals and bioeroders, such as excavating sponges, with broad implications for the balance between biologically mediated deposition and erosion of carbonate in reef communities. This study investigated the effects of three levels of CO₂ (present-day, mid-century and end-of-century projections) on the direct interaction between a bioeroding sponge, Cliona varians, and a Caribbean coral, Porites furcata. Increased pCO₂ concentrations had no effect on the attachment rates of C. varians to the corals, and we observed no significant impact of pCO₂ on the survival of either the coral or sponges. However, exposure to end-of-century levels of CO₂-dosing (~750 μatm) reduced calcification in P. furcata and led to a significant increase in sponge-mediated erosion of P. furcata. These findings demonstrate that pCO₂ can enhance erosional efficiency without impacting survival or competitive vigor in these two species. While few studies have considered the influence of pCO₂ on the competitive outcomes of interactions between corals and other reef organisms, our study suggests that assessing the impacts of changing pCO₂ on species interactions is crucial to adequately predict ecosystem-level responses in the future.     

Effect of biochar addition on short-term N<Subscript>2</Subscript>O and CO<Subscript>2</Subscript> emissions during repeated drying and wetting of an anthropogenic alluvial soil

Agricultural soils are an important source of greenhouse gases (GHG). Biochar application to such soils has the potential of mitigating global anthropogenic GHG emissions. Under irrigation, the topsoils in arid regions experience repeated drying and wetting during the crop growing season. Biochar incorporation into these soils would change the soil microbial environment and hence affect GHG emissions. Little information, however, is available regarding the effect of biochar addition on carbon dioxide (CO₂) and nitrous oxide (N₂O) emissions from agricultural soils undergoing repeated drying and wetting. Here, we report the results of a 49-day aerobic incubation experiment, incorporating biochar into an anthropogenic alluvial soil in an arid region of Xinjiang Province, China, and measuring CO₂ and N₂O emissions. Under both drying–wetting and constantly moist conditions, biochar amendment significantly increased cumulative CO₂ emission. At the same time, there was a significant reduction (up to ~20 %) in cumulative N₂O emission, indicating that the addition of biochar to irrigated agricultural soils may effectively slow down global warming in arid regions of China.     

Tough as a rock-boring urchin: adult Echinometra sp. EE from the Red Sea show high resistance to ocean acidification over long-term exposures

Ocean acidification, a process caused by the continuous rise of atmospheric CO₂ levels, is expected to have a profound impact on marine invertebrates. Findings of the numerous studies conducted in this field indicate high variability in species responses to future ocean conditions. This study aimed at understanding the effects of long-term exposure to elevated pCO₂ conditions on the performance of adult Echinometra sp. EE from the Gulf of Aqaba (Red Sea). During an 11-month incubation under high pCO₂ (1,433 μatm, pH_NBS 7.7) and control (435 μatm, pH_NBS 8.1) conditions, we examined the urchins’ somatic and gonadal growth, gametogenesis and skeletal microstructure. Somatic and gonadal growths were exhibited with no significant differences between the treatments. In addition, all urchins in the experiment completed a full reproductive cycle, typical of natural populations, with no detectable impact of increased pCO₂ on the timing, duration or progression of the cycle. Furthermore, scanning electron microscopy imaging of urchin tests and spines revealed no signs of the usual observed effects of acidosis, such as skeletal dissolution, widened stereom pores or non-smoothed structures. Our results, which yielded no significant impact of the high pCO₂ treatment on any of the examined processes in the urchins studied, suggest high resistance of adult Echinometra sp. EE to near future ocean acidification conditions. With respect to other findings in this area, the outcome of this study provides an example of the complicated and diverse responses of echinoids to the predicted environmental changes.     

Ocean acidification and warming alter photosynthesis and calcification of the symbiont-bearing foraminifera Marginopora vertebralis

The impact of elevated CO₂ and temperature on photosynthesis and calcification in the symbiont-bearing benthic foraminifer Marginopora vertebralis was studied. Individual specimens of M. vertebralis were collected from Heron Island on the southern Great Barrier Reef (Australia). They were maintained for 5 weeks at different temperatures (28, 32 °C) and pCO₂ (400, 1,000 µatm) levels spanning a range of current and future climate-change scenarios. The photosynthetic capacity of M. vertebralis was measured with O₂ microsensors and a pulse-amplitude-modulated chlorophyll (Chl) fluorometer, in combination with estimates of Chl a and Chl c ₂ concentrations and calcification rates. After 5 weeks, control specimens remained unaltered for all parameters. Chlorophyll a concentrations significantly decreased in the specimens at 1,000 µatm CO₂ for both temperatures, while no change in Chl c ₂ concentration was observed. Photoinhibition was observed under elevated CO₂ and temperature, with a 70–80 % decrease in the maximum quantum yield of PSII. There was no net O₂ production at elevated temperatures in both CO₂ treatments as compared to the control temperature, supporting that temperature has more impact on photosynthesis and O₂ flux than changes in ambient CO₂. Photosynthetic pigment loss and a decrease in photochemical efficiency are thus likely to occur with increased temperature. The elevated CO₂ and high temperature treatment also lead to a reduction in calcification rate (from +0.1 to >?0.1 % day^?1). Thus, both calcification and photosynthesis of the major sediment-producing foraminifer M. vertebralis appears highly vulnerable to elevated temperature and ocean acidification scenarios predicted in climate-change models.     

Structural and functional vulnerability to elevated pCO2 in marine benthic communities

The effect of elevated pCO₂/low pH on marine invertebrate benthic biodiversity, community structure and selected functional responses which underpin ecosystem services (such as community production and calcification) was tested in a medium-term (30 days) mesocosm experiment in June 2010. Standardised intertidal macrobenthic communities, collected (50.3567°N, 4.1277°W) using artificial substrate units (ASUs), were exposed to one of seven pH treatments (8.05, 7.8. 7.6, 7.4, 7.2, 6.8 and 6.0). Community net calcification/dissolution rates, as well as changes in biomass, community structure and diversity, were measured at the end of the experimental period. Communities showed significant changes in structure and reduced diversity in response to reduced pH: shifting from a community dominated by calcareous organisms to one dominated by non-calcareous organisms around either pH 7.2 (number of individuals and species) or pH 7.8 (biomass). These results were supported by a reduced total weight of CaCO₃ structures in all major taxa at lowered pH and a switch from net calcification to net dissolution around pH 7.4 (Ω_calc = 0.78, Ω_ara = 0.5). Overall community soft tissue biomass did not change with pH and high mortality was observed only at pH 6.0, although molluscs and arthropods showed significant decreases in soft tissue. This study supports and refines previous findings on how elevated pCO₂ can induce changes in marine biodiversity, underlined by differential vulnerability of different phyla. In addition, it shows significant elevated pCO₂-/low pH-dependent changes in fundamental community functional responses underpinning changes in ecosystem services.     

Effect of increasing sea water pCO2 on the northern Atlantic krill species Nyctiphanes couchii

Surprisingly little is known about potential effects of ocean acidification on krill of the Northern Hemisphere as ecologically very important food web component. Sub-adult individuals of the northern Atlantic krill species Nyctiphanes couchii (caught at Austevoll near Bergen, Norway, in January 2013) were exposed in the laboratory to four different levels of pCO₂ (430, 800, 1,100, and 1,700 µatm) for 5 weeks in order to assess potential changes in a set of biological response variables. Survival decreased and the frequency of moulting-related deaths increased with increasing pCO₂. Survival was considerably reduced at relatively high pCO₂ of 1,700 µatm and tended to be negatively affected at 1,100 µatm pCO₂. However, the experimental results show no significant effects of pCO₂ on inter-moult period and growth at pCO₂ levels below 1,100 µatm. No differences in length measurements of the carapace and uropod were observed across pCO₂ levels, indicating no effect of changing carbonate chemistry on the morphology of those calciferous parts of the exoskeleton. The results suggest that sub-adult N. couchii may not suffer dramatically from predicted near-future changes in pCO₂. However, potential detrimental effects on the moulting process and associated higher mortality at 1,100 µatm pCO₂ cannot be excluded. Further experiments are needed in order to investigate whether early life stages of N. couchii show a different sensitivity to elevated sea water pCO₂ and whether those results are transferable to other krill species of the Northern Hemisphere.     

Elevated CO2 affects the behavior of an ecologically and economically important coral reef fish

We tested the effect of near-future CO₂ levels (≈490, 570, 700, and 960 μatm CO₂) on the olfactory responses and activity levels of juvenile coral trout, Plectropomus leopardus, a piscivorous reef fish that is also one of the most important fisheries species on the Great Barrier Reef, Australia. Juvenile coral trout reared for 4 weeks at 570 μatm CO₂ exhibited similar sensory responses and behaviors to juveniles reared at 490 μatm CO₂ (control). In contrast, juveniles reared at 700 and 960 μatm CO₂ exhibited dramatically altered sensory function and behaviors. At these higher CO₂ concentrations, juveniles became attracted to the odor of potential predators, as has been observed in other reef fishes. They were more active, spent less time in shelter, ventured further from shelter, and were bolder than fish reared at 490 or 570 μatm CO₂. These results demonstrate that behavioral impairment of coral trout is unlikely if pCO₂ remains below 600 μatm; however, at higher levels, there are significant impacts on juvenile performance that are likely to affect survival and energy budgets, with consequences for predator–prey interactions and commercial fisheries.     

地质封存CO_2泄漏对蚯蚓的毒性效应

二氧化碳捕集与封存技术(CO_2 capture and storage, CCS)是当前国际上公认的CO_2减排的有效措施,但封存在地下的CO_2仍然因为各种不稳定因素存在泄漏风险,对土壤环境及土壤生态系统产生威胁。选择赤子爱胜蚓为研究对象,通过模拟高浓度CO_2对蚯蚓形态与生理变化的影响,探究CCS泄漏所产生的土壤高浓度CO_2对蚯蚓的毒性效应。研究表明,土壤高浓度CO_2使蚯蚓出现生殖环带肿大、尾部串珠以及断尾等外部形态变化,皮肤和刚毛受到损伤并且表皮发生褶皱等现象;随着CO_2浓度的增加以及暴露时间的延长,蚯蚓的死亡率不断增加,土壤高浓度CO_2对蚯蚓的7 d和14 d半致死浓度分别为26.39%和17.78%;蚯蚓体腔细胞溶酶体中性红保留时间(NRRT)减少。因此,蚯蚓有望作为监测CO_2泄漏的指示生物,NRRT可作为识别CO_2泄漏的敏感指标。     

Integrierte Treibhausgasbewertung der Prozessketten von Erdgas und industriellem Biomethan in Deutschland

Background The use of natural gas has increased in the last years. In the future, its import supply and transport structure will diversify (longer distances, higher share of LNG (liquefied natural gas), new pipelines). Thus the process chain and GHG emissions of the production, processing, transport and distribution might change. Simultaneously, the injection of bio methane into the natural gas grid is becoming more important. Although its combustion is regarded as climate neutral, during the production processes of bio methane GHG emissions are caused. The GHG emissions occurring during the process chain of energy fuels are relevant for the discussion on climate policy and decision making processes. They are becoming even more important, considering the new Fuel Quality Directive of the EU (Dec. 2008), which aims at controlling emissions of the fuel process chains. Aim In the context of the aspects outlined above the aim is to determine the future development of gas supply for Germany and the resulting changes in GHG emissions of the whole process chain of natural gas and bio methane. With the help of two gas consumption scenarios and an LCA of bio methane, the amount of future emissions and emission paths until 2030 can be assessed and used to guide decision processes in energy policy. Results and discussion The process chain of bio methane and its future technical development are outlined and the related emissions calculated. The analysis is based on an accompanying research study on the injection of bio methane to the German gas grid. Two types of biogas plants have been considered whereof the “optimised technology” is assumed to dominate the future market. This is the one which widely exploits the potential of process optimisation of the current “state of the art” plant. The specific GHG emissions of the process chain can thus be nearly halved from currently 27.8?t CO₂-eq./TJ to 14.8?t CO₂-eq./TJ in 2030. GHG emissions of the natural gas process chain have been analysed in detail in a previous article. Significant modifications and a decrease of specific emissions is possible, depending on the level of investment in the modernisation of the gas infrastructure and the process improvements. These mitigation options might neutralise the emission increase resulting from longer distances and energy intensive processes. In the last section two scenarios (low and high consumption) illustrate the possible development of the German gas supply until 2030, given an overall share of 8–12?% of bio methane. Considering the dynamic emission factors calculated in the former sections, the overall gas emissions and average specific emissions of German gas supply can be given. The current emissions of 215.4 million t CO₂-eq. are reduced by 25?% in the low-consumption scenario (162 million t CO₂-eq.), where consumption is reduced by 17?%. Assuming a consumption which is increased by 17?% in 2030, emissions are around 7?% higher (230.9 million t CO₂-eq.) than today. Conclusions Gaseous fuels will still play a significant role for the German energy supply in the next two decades. The GHG emissions mainly depend on the amount of gas used. Thus, energy efficiency will be a key issue in the climate and energy related policy discussion. A higher share of bio methane and high investments in mitigation and best available technologies can significantly reduce the emissions of the process chain. The combustion of bio methane is climate neutral compared to 56?t CO₂/TJ caused by the direct combustion of natural gas (or 111?t CO₂/TJ emitted by lignite). The advantage of gaseous energy carriers with the lowest levels of GHG emissions compared to other fossil fuels still remains. This holds true for fossil natural gas alone as well as for the expected future blend with bio-methane.     

Physiological responses of the calcifying rhodophyte, <Emphasis Type="Italic">Corallina officinalis</Emphasis> (L.), to future CO<Subscript>2</Subscript> levels

Future atmospheric CO₂ levels will most likely have complex consequences for marine organisms, particulary photosynthetic calcifying organisms. Corallina officinalis L. is an erect calcifying macroalga found in the inter- and subtidal regions of temperate rocky coastlines and provides important substrate and refugia for marine meiofauna. The main goal of the current study was to determine the physiological responses of C. officinalis to increased CO₂ concentrations expected to occur within the next century and beyond. Our results show that growth and production of inorganic material decreased under high CO₂ levels, while carbonic anhydrase activity was stimulated and negatively correlated to algal inorganic content. Photosynthetic efficiency based on oxygen evolution was also negatively affected by increased CO₂. The results of this study indicate that C. officinalis may become less competitive under future CO₂ levels, which could result in structural changes in future temperate intertidal communities.     

Within- and transgenerational effects of ocean acidification on life history of marine three-spined stickleback (Gasterosteus aculeatus)

Some studies have demonstrated that elevated CO₂ concentrations in ocean waters negatively impact metabolism and development of marine fish. Particularly, early developmental stages are probably more susceptible to ocean acidification due to insufficient regulations of their acid-base balance. Transgenerational acclimation can be an important mechanism to mediate impacts of increased CO₂ on marine species, yet very little is known about the potential of parental effects in teleosts. Therefore, transgenerational effects were investigated on life history in juvenile three-spined sticklebacks Gasterosteus aculeatus by acclimating parents (collected in April 2012, 55°03′N, 8°44′E) and offspring to ambient (~400 µatm) and elevated (~1,000 µatm) CO₂ levels and measured parental fecundity as well as offspring survival, growth and otolith characteristics. Exposure to elevated CO₂ concentrations led to an increase in clutch size in adults as well as increased juvenile survival and growth rates between 60 and 90 days post-hatch and enlarged otolith areas compared with fish from ambient CO₂ concentrations. Moreover, transgenerational effects were observed in reduced survival and body size 30 days post-hatch as well as in enlarged otoliths at the end of the experiment, when fathers or both parents were acclimated to the high-CO₂ environment. These results may suggest that elevated CO₂ concentrations had rather positive effects on life-history traits of three-spined sticklebacks, but that parental acclimation can modify these effects without improving offspring fitness. Although the mechanistic basis of such transgenerational acclimation remains unclear, selective gradients within generations seem to determine the direction of transgenerational effects.     

Effects of ocean acidification on invertebrate settlement at volcanic CO2 vents

We present the first study of the effects of ocean acidification on settlement of benthic invertebrates and microfauna. Artificial collectors were placed for 1 month along pH gradients at CO₂ vents off Ischia (Tyrrhenian Sea, Italy). Seventy-nine taxa were identified from six main taxonomic groups (foraminiferans, nematodes, polychaetes, molluscs, crustaceans and chaetognaths). Calcareous foraminiferans, serpulid polychaetes, gastropods and bivalves showed highly significant reductions in recruitment to the collectors as pCO₂ rose from normal (336–341 ppm, pH 8.09–8.15) to high levels (886–5,148 ppm) causing acidified conditions near the vents (pH 7.08–7.79). Only the syllid polychaete Syllis prolifera had higher abundances at the most acidified station, although a wide range of polychaetes and small crustaceans was able to settle and survive under these conditions. A few taxa (Amphiglena mediterranea, Leptochelia dubia, Caprella acanthifera) were particularly abundant at stations acidified by intermediate amounts of CO₂ (pH 7.41–7.99). These results show that increased levels of CO₂ can profoundly affect the settlement of a wide range of benthic organisms.     

Increased carbon dioxide availability alters phytoplankton stoichiometry and affects carbon cycling and growth of a marine planktonic herbivore

Rising levels of CO₂ in the atmosphere have led to increased CO₂ concentrations in the oceans. This enhanced carbon availability to the marine primary producers has the potential to change their nutrient stoichiometry, and higher carbon-to-nutrient ratios are expected. As a result, the quality of the primary producers as food for herbivores may change. Here, we present experimental work showing the effect of feeding Rhodomonas salina grown under different pCO₂ (200, 400 and 800 μatm) on the copepod Acartia tonsa. The rate of development of copepodites decreased with increasing CO₂ availability to the algae. The surplus carbon in the algae was excreted by the copepods, with younger stages (copepodites) excreting most of their surplus carbon through respiration and adult copepods excreting surplus carbon mostly as DOC. We consider the possible consequences of different excretory pathways for the ecosystem. A continued increase in the CO₂ availability for primary production, together with changes in the nutrient loading of coastal ecosystems, may cause changes in the trophic links between primary producers and herbivores.     

Future ocean acidification will be amplified by hypoxia in coastal habitats

Ocean acidification is elicited by anthropogenic carbon dioxide emissions and resulting oceanic uptake of excess CO₂ and might constitute an abiotic stressor powerful enough to alter marine ecosystem structures. For surface waters in gas-exchange equilibrium with the atmosphere, models suggest increases in CO₂ partial pressure (pCO₂) from current values of ca. 390 μatm to ca. 700–1,000 μatm by the end of the century. However, in typically unequilibrated coastal hypoxic regions, much higher pCO₂ values can be expected, as heterotrophic degradation of organic material is necessarily related to the production of CO₂ (i.e., dissolved inorganic carbon). Here, we provide data and estimates that, even under current conditions, maximum pCO₂ values of 1,700–3,200 μatm can easily be reached when all oxygen is consumed at salinities between 35 and 20, respectively. Due to the nonlinear nature of the carbonate system, the approximate doubling of seawater pCO₂ in surface waters due to ocean acidification will most strongly affect coastal hypoxic zones as pCO₂ during hypoxia will increase proportionally: we calculate maximum pCO₂ values of ca. 4,500 μatm at a salinity of 20 (T = 10 °C) and ca. 3,400 μatm at a salinity of 35 (T = 10 °C) when all oxygen is consumed. Upwelling processes can bring these CO₂-enriched waters in contact with shallow water ecosystems and may then affect species performance there as well. We conclude that (1) combined stressor experiments (pCO₂ and pO₂) are largely missing at the moment and that (2) coastal ocean acidification experimental designs need to be closely adjusted to carbonate system variability within the specific habitat. In general, the worldwide spread of coastal hypoxic zones also simultaneously is a spread of CO₂-enriched zones. The magnitude of expected changes in pCO₂ in these regions indicates that coastal systems may be more endangered by future global climate change than previously thought.     

CO2 leakage simulation: effects of the pH decrease on fertilisation and larval development of Paracentrotus lividus and sediment metals toxicity

Carbon capture and storage has become a new mitigation option to reduce anthropogenic CO₂ emissions. The effects of the CO₂-related acidification, associated with unpredictable leaks of CO₂ during the operational phases were studied using the Paracentrotus lividus sea urchin-liquid-phase-assays (fertilisation and embryo-larval development tests). Besides to the direct effects of the CO₂-associated pH decrease, the possible effects on marine sediment toxicity were studied by exposing the urchin larvae to elutriate of sediments with different metals concentration, which were subjected to various pH treatments. The resulted median toxic effect pH ranged from 6.33?±?0.02 and 6.91?±?0.01 for the egg fertilisation, and between 6.66?±?0.03 and 7.16?±?0.01 for the larval development assays. Concentrations of Co, Zn, As, Cu, and Fe were detected in the sediment elutriates. For all the sediment elutriates tested the amount of the dissolved Zn increased in parallel with the pH reductions. Correlated effects of acidification, biological response, and the presence of dissolved metals were observed in this work evidencing that the most important factor controlling the reduction of egg fertilisation and larval development success was the combined effects of the reduction of pH and the presence of the contaminants (mainly Zn, Co, and As).     

Tolerance of juvenile barnacles (Amphibalanus improvisus) to warming and elevated pCO2

We investigated the impacts of warming and elevated pCO₂ on newly settled Amphibalanus improvisus from Kiel Fjord, an estuarine ecosystem characterized by significant natural pCO₂ variability. In two experiments, juvenile barnacles were maintained at two temperature and three pCO₂ levels (20/24 °C, 700–2,140 μatm) for 8 weeks in a batch culture and at four pCO₂ levels (20 °C, 620–2,870 μatm) for 12 weeks in a water flow-through system. Warming as well as elevated pCO₂ hardly affected growth or the condition index of barnacles, although some factor combinations led to temporal significances in enhanced or reduced growth with an increase in pCO₂. While warming increased the shell strength of A. improvisus individuals, elevated pCO₂ had only weak effects. We demonstrate a strong tolerance of juvenile A. improvisus to mean acidification levels of about 1,000 μatm pCO₂ as is already naturally experienced by the investigated barnacle population.     

Effects of boreal forest management practices on the climate impact of CO2 emissions from bioenergy

In Life Cycle Assessment (LCA), carbon dioxide (CO₂) emissions from biomass combustion are traditionally assumed climate neutral if the bioenergy system is CO₂ flux neutral, i.e. the quantity of CO₂ released approximately equals the amount of CO₂ sequestered in biomass. This convention is a plausible assumption for fast growing biomass species, but is inappropriate for slower growing biomass, like forests. In this case, the climate impact from biomass combustion can be potentially underestimated if CO₂ emissions are ignored, or overestimated, if biogenic CO₂ is considered equal to anthropogenic CO₂. The estimation of the effective climate impact should take into account how the CO₂ fluxes are distributed over time: the emission of CO₂ from bioenergy approximately occurs at a single point in time, while the absorption by the new trees is spread over several decades. Our research target is to include this dynamic time dimension in unit-based impact analysis, using a boreal forest stand as case study. The boreal forest growth is modelled with an appropriate function, and is investigated under different forestry regimes (affecting the growth rate and the year of harvest). Specific atmospheric decay functions for biomass-derived CO₂ are then elaborated for selected combinations of forest management options. The contribution to global warming is finally quantified using the GWP_bio index as climate metric. Results estimates the effects of these practices on the characterization factor used for the global warming potential of CO₂ from bioenergy, and point out the key role played by the selected time horizon.     

Evaluation of numerical simulations of CO2 transport in a city block with field measurements

Studying urban air-transport phenomena is highly complex, because of the heterogenous flow patterns that can arise. The main reason for these is the variable topology of urban areas, however, there is a large number of influencing variables such as meteorological conditions (e.g., wind situation, temperature) and anthropogenic factors such as traffic emissions. During a one-year CO₂ measurement campaign in the city of Basel, Switzerland, steep CO₂ gradients were measured around a large building. The concentration differences showed a strong dependency on the local flow regimes. Analysis of the field data alone did not provide a complete explanation for the mechanisms underlying the observed phenomena. The key numerical parameters were defined and the influence of turbulent kinetic energy dependency on the time interval for the Reynolds decomposition was studied. A Reynolds-Average Navier-Stokes Computational Fluid Dynamics (CFD) approach was applied in the study area and the CO₂ concentrations were simulated for six significant meteorological situations and compared to the measured data. Two flow regimes dependent on the wind situation, which either enhanced or suppressed the concentration of CO₂ in the street canyon, were identified. The enhancement of CO₂ in the street canyon led to a large difference in CO₂ concentration between the backyard- and street-sides of a building forming the one wall of the canyon. The specific characteristics of the flow patterns led to the identification of the processes determining the observed differences in CO₂ concentrations. The combined analysis of measurement and modeling showed the importance of reliable field measurements and CFD simulations with a high spatial resolution to assess transport mechanisms in urban areas.     

Effects of long-term acclimation to environmental hypercapnia on extracellular acid–base status and metabolic capacity in Mediterranean fish <Emphasis Type="Italic">Sparus aurata</Emphasis>

In the context of future scenarios of anthropogenic CO₂ accumulation in marine surface waters, the present study addresses the effects of long-term hypercapnia on a Mediterranean fish, Sparus aurata. By equilibration with elevated CO₂ levels seawater pH was lowered to a value of 7.3, close to the maximum pH drop expected in marine surface waters from atmospheric CO₂ accumulation. Intra- and extracellular acid–base parameters as well as changes in enzyme profiles were studied in red and white muscles and the heart under both normocapnia and hypercapnia. The activities of pyruvate kinase (PK), lactate dehydrogenase (L-LDH), citrate synthase (CS), malate dehydrogenase and and 3-hydroxyacyl CoA dehydrogenase (HOAD) reflect the pathways and capacity of oxidative processes in metabolism. Long-term hypercapnia caused a transient reduction in blood plasma pH (pH_e) as well as in intracellular pH (pH_i). Compensation of the acidosis occurred through increased plasma and cellular bicarbonate levels. Changes in enzymatic activities, especially the increase in the activity of L-LDH, paralleled by a drop in CS activity in white and red muscles reflect a shift from aerobic to anaerobic pathways of substrate oxidation during long-term acclimation under hypercapnia. The present results suggest that moderate environmental hypercapnia changes the metabolic profile in tissues of S. aurata. Consequences for slow processes like growth and reproduction potential as well as potential harm at population, species and ecosystem levels require further investigation.     

The physiological and molecular responses of larvae from the reef-building coral Pocillopora damicornis exposed to near-future increases in temperature and pCO2

Given the threats of greenhouse gas emissions and a changing climate to marine ecosystems, there is an urgent need to better understand the response of not only adult corals, which are particularly sensitive to environmental changes, but also their larvae, whose mechanisms of acclimation to both temperature increases and ocean acidification are not well understood. Brooded larvae from the reef coral Pocillopora damicornis collected from Nanwan Bay, Southern Taiwan, were exposed to ambient or elevated temperature (25 or 29 °C) and pCO₂ (415 or 635 μatm) in a factorial experiment for 9 days, and a variety of physiological and molecular parameters were measured. Respiration and rubisco protein expression decreased in larvae exposed to elevated temperature, while those incubated at high pCO₂ were larger in size. Collectively, these findings highlight the complex metabolic and molecular responses of this life history stage and the need to integrate our understanding across multiple levels of biological organization. Our results also suggest that for this pocilloporid larval life stage, the impacts of elevated temperature are likely a greater threat under near-future predictions for climate change than ocean acidification.