Distribution,life history,and production of three species of <Emphasis Type="Italic">Neomysis</Emphasis> in Akkeshi-ko estuary,northern Japan

Spatial and seasonal distribution pattern, life history and production of three species of Neomysis (Mysidacea) which commonly occur in northwestern subarctic Pacific coastal waters, were investigated throughout the year in the Akkeshi-ko estuary, northern Japan. The most abundant species Neomysis awatschensis (annual mean density: 179.8 inds. m⁻², biomass: 108.8 mg DW m⁻²) occurred at the inner part of the estuary including low salinity areas with no clear preference for the seagrass bed. The second most abundant Neomysis mirabilis (mean density: 95.8 inds. m⁻², biomass: 90.1 mg DW m⁻²) occurred at relatively saline seagrass site throughout the year. Occurrence of Neomysis czerniawskii in the estuary was limited to the seagrass bed during summer when their population mainly consisted of juveniles, suggesting that this species is a seasonal migrant between the estuary and the marine environment. Both N. awatschensis and N. mirabilis populations were composed of two generation types, a larger sized overwintering and smaller sized spring/summer generations; however, each species had a different reproductive strategy. N. awatschensis was characterized by fast growth to maturity at a smaller size than N. mirabilis with a relatively high fecundity during warm season, suggesting that this species is an r-strategist which can utilize opportunistically a wide variety of habitats. In contrast, the seagrass bed resident N. mirabilis was a K-strategist which matures at a larger size producing fewer but larger offspring. The annual production of N. awatschensis (0.57–0.70 g DW m⁻², mean of the whole estuary) and N. mirabilis (0.58–0.68 g DW m⁻², mean of the seagrass bed) at their respective habitats was comparable. Consequently, species-specific life history and distribution pattern are concluded to allow Neomysis spp. to coexist in the estuary and the high carrying capacity of seagrass bed is suggested to contribute to maintain their high biomass level.     

Variation in measures of immunocompetence of sea fan coral, <Emphasis Type="Italic">Gorgonia ventalina</Emphasis>, in the Florida Keys

Aspergillosis is a widespread disease that has impacted the demography of the Caribbean sea fan coral, Gorgonia ventalina. The innate coral immune defenses can be measured as constitutive levels of immune proteins (peroxidase [POX], prophenoloxidase [PPO], lysozyme-like activity [LYS], exochitinase [EXOC]), antioxidant (superoxide dismutase [SOD]), and antimicrobial (antibacterial [AB] and antifungal [AF]) activity. Therefore, variations in these parameters across a geographic region could provide clues to the role of environment in disease. This study examined healthy sea fans collected in July 2005 from six offshore sites in the Florida Keys lying between 24.569°N and 25.220°N, a distance of ~145 km. Contrary to expectations, small (<15 cm) colonies did not differ significantly from large colonies (>15 cm) in the protein-based levels of activity in any of the measured parameters. However, there were significant differences in many of the parameters among sites, and Molasses Reef and Looe Key Reef were the most different in POX, PPO, SOD, and AF activity. This suggests that there are potential site-specific environmental factors that shape the immune physiology of colonies. Several proxies of environmental stress were also regressed against levels of the immune parameters. The proxies included 10 year averages of benthic community composition, 5 year averages of water quality, and historic aspergillosis disease prevalence and severity. Generality about environmental drivers was limited by assaying only six sites, but several patterns did emerge. SOD, EXOC, and AF activity were all correlated with percent bare substrate cover, suggesting that certain immune components may be activated in low coral environments. LYS and EXOC activity were positively correlated with dissolved inorganic nitrogen (DIN), one proxy of water quality. There were no relationships between any of the measured immune parameters and previous disease prevalence and severity. This study is a first step in evaluating levels of within- and between-site variation in coral immunity and investigating possible environmental drivers.     

Activity patterns of Kemp's ridley turtles, <Emphasis Type="Italic">Lepidochelys kempii</Emphasis>, in the coastal waters of the Cedar Keys,Florida

Radio and sonic telemetry were used to investigate the tidal orientation, rate of movement (ROM), and surfacing behavior of nine Kemp's ridley turtles, Lepidochelys kempii, tracked east of the Cedar Keys, Florida. The mean of mean turtle bearings on incoming (48ᇅ °) and falling (232ᆽ °) tides was significantly oriented to the mean directions of tidal flow (37Nj °, P<0.0025, and 234Nj °, P<0.005, respectively). Turtles had a mean ROM of 0.44ǂ.33 km/h (range: 0.004-1.758 km/h), a mean surface duration of 18ᆣ s (range: 1-88 s), and a mean submergence duration of 8.4Lj.4 min (range: 0.2-60.0 min). ROM was negatively correlated with surface and submergence durations and positively correlated with the number of surfacings. Furthermore, ROMs were higher and surface and submergence durations were shorter during the day. Daily activities of turtles were attributed to food acquisition and bioenergetics.     

Population structure of red porgy, <Emphasis Type="Italic">Pagrus pagrus</Emphasis>, in the Atlantic Ocean

The red porgy, Pagrus pagrus (L.), is a protogynous sparid associated with reefs and hard bottom habitat throughout the warm-temperate Atlantic Ocean. In this study, the degree of geographic population differentiation in Atlantic populations was examined with microsatellite and mitochondrial DNA markers (mtDNA). Six microsatellite loci were amplified and scored in 690 individuals from the eastern North Atlantic (Crete, Madeira, and Azores), western North Atlantic (North Carolina to Florida, and the eastern Gulf of Mexico), and Brazil. At two loci, fixed allelic differences were found among the three major geographic areas, while frequency differences were observed at three other loci. The DNA of 371 individuals was amplified at the mtDNA control region, and 526 bp were sequenced. Tamura–Nei’s D was used as a measure of nucleotide diversity and divergence: diversity averaged 0.011 within samples, while the corrected divergence averaged 0 between samples within the same area and 0.061 between samples from different areas. Transversion haplotype minimum spanning networks, nucleotide divergence, and F _ST values all show that the western Atlantic samples were more closely related to each other than any was to samples from the eastern North Atlantic. Within the western North Atlantic, no significant population differentiation was observed, and within the eastern North Atlantic, only the Azores sample showed detectable differences from Crete and Madeira. These data indicate general homogeneity within large areas, and deep divisions between these areas. Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users.     

Temporal and spatial variation in protein,carbohydrate, and lipid levels in <Emphasis Type="Italic">Corallium rubrum</Emphasis> (Anthozoa,Octocorallia)

Red coral (Corallium rubrum) is an important component of the Mediterranean coralligenous habitat. The present study analyzes the relationship between energy storage molecule concentration (i.e. tissue balance of protein–carbohydrate–lipid levels) and the gonadal output in two populations located at different depths (16–18 m and 40–45 m depth) through a seasonal cycle (2002–2003). In June 2003, another six populations have been sampled to compare medium-scale geographical variation. The colonies sampled were less than 6 cm high, thus representing the majority of the population in the studied area. There was no clear relationship between energy storage (i.e. carbohydrate and lipid levels) and gonadal output, nor in the seasonal cycle or the spatial variation. A clear-cut seasonal trend of energy storage was detected only in the shallower population. The water temperature was warmer at 20 m depth and the water transparency (Secchi disk) was also higher (lower seston concentration) in 2003 when the same periods in 2002 and 2003 were compared (May–August). Carbohydrates seemed to be more sensitive to food constraints than lipids. The partial mortality detected in summer 2003 in the sampling area appeared to be recorded in the carbohydrate and lipid levels of this species, especially in the shallow population. The biochemical levels prove to be a useful tool to detect unusual environmental conditions that may cause partial or total mortality in benthic suspension feeders.     

Life history of a dominant polychaete, <Emphasis Type="Italic">Polygordius jouinae</Emphasis>, in inner continental shelf sands of the Mid-Atlantic Bight,USA

The life history of the polychaete Polygordius jouinae Ramey, Fiege, and Leander 2006 was studied in the sandy sediments of the LEO-15 research area off the coast of Tuckerton, New Jersey (39°27.69′N, 74°15.81′W). Divers collected sediment cores biweekly, from February 2004 to November 2005 to examine the temporal patterns of abundance and size–frequency distributions of P. jouinae. These parameters were also examined (for the first time) for P. jouinae in samples taken in ambient sediments and in three reciprocal 3 to 5-day sediment transplant experiments that were part of a previous, unrelated study at coarse sand and muddy sand sites (∼3 km apart) at LEO-15 in the summers of 1994 and 1995. Analysis of these previous data along with the current data provided a larger set of values for P. jouinae, as well as further insight into the life history, habitat selection, and dispersal of P. jouinae (not considered in previous studies). Polygordius jouinae is gonochoristic and the population was mostly composed of sexually mature individuals in late May 2004 and 2005. The reproductive period usually occurs from May to August and individuals that spawn generally have a 1-year life span. Non-spawning individuals of P. jouinae could potentially live longer than 1 year and therefore the population may sometimes consist of individuals of two generations. Recruitment begins no later than July as recently settled individuals (≤9 mm body length), which were not present during the first week of June 1995, 2004, and 2005, first appeared in July in all 3 years. The smallest individual was 2.01 mm long and 0.07 mm wide and this provides the first estimate of size at initial recruitment. Although some worms in sediment trays likely settled as larvae, most worms were recently settled juveniles (length categories 6 and 9 mm) transported in from the surrounding sediments, presumably as a consequence of re-suspension or bed load transport. As expected, larger individuals were present later in September. Although it is unknown whether P. jouinae were transported actively or passively these data suggest that recently settled juveniles are capable of habitat selection (prefer sand over mud) and show that post-settlement transport and selection influence community structure.     

Eye development in southern calamary, <Emphasis Type="Italic">Sepioteuthis australis</Emphasis>, embryos and hatchlings

Eye development, optical properties and photomechanical responses were examined in embryos and hatchlings of the southern calamary, Sepioteuthis australis. This species occurs in shallow coastal waters in Australia and New Zealand, and the egg masses were collected in October and December 2004 from Great Oyster Bay, Tasmania. At the earliest developmental stage the eye of the squid was comprised of a hemispherical cup of undifferentiated neural retina, while presumptive iris cell layers and lentigenic precursor cells enclosed a posterior eye chamber. Differentiation of the proximal and distal processes was observed in correspondence with the cornea development and lens crystallization, and occurred before differentiation of the neural retina, which was complete prior to hatching. Longer photoreceptor distal processes were first observed just prior to hatching in the dorsal-posterior retina. After hatching, this difference was much more evident and higher photoreceptor density was found in the central retina. This indicates that the eye of S. australis at this age uses different retina areas for different visual tasks. Optical sensitivity and resolution suggest that juvenile S. australis are diurnal. This study also found functional photomechanical responses of visual screening pigment migration and pupil constriction in S. australis embryos, although complete functionality of the pupil at this stage was uncertain. However, the pupils of squid aged 2 days closed almost completely under bright conditions, showing that photomechanical responses were highly developed in the juvenile squid. These findings indicate that squid embryos are able to perceive visual stimulation, suggesting an early reliance on vision for survival after hatching.     

Biotic resistance to green crab, <Emphasis Type="Italic">Carcinus maenas</Emphasis>, in California bays

The distribution of the introduced European green crab, Carcinus maenas, was investigated in the central California embayments of Bodega Bay Harbor (BBH), Tomales Bay, and Bolinas Lagoon using baited traps and snorkel surveys. Adult green crabs were very spatially limited in all three embayments and occurred primarily in warm, shallow areas that lacked large native Cancer spp. crabs. The green crabs that were found in closest proximity to populations of Cancer spp. exhibited high levels of limb damage and loss; damage was strongly correlated with low ratios of intertidal area: edge, indicative of narrow areas of intertidal that are more easily accessed by large Cancer spp. moving up to forage during periods of tidal inundation. Up to 70% of the green crabs tethered in areas of BBH that are utilized by Cancer spp. experienced limb loss, while those tethered in the marsh, where there are no Cancer spp., were undamaged. The results suggest that the potential distribution of green crabs in the northeastern Pacific will be far less than has been predicted, and that their impacts may be largely attenuated through predation by and competition with native crab species.     

Maturity,fecundity, and reproductive cycle of the spotted ratfish, <Emphasis Type="Italic">Hydrolagus colliei</Emphasis>

Size at maturity, fecundity, and reproductive periodicity were estimated for the spotted ratfish, Hydrolagus colliei (Lay and Bennett, 1839), off the coast of California, Oregon, and Washington. Maximum body size and size at median maturity were greater for females than males. Skeletal muscle concentrations of the steroid hormones testosterone (T) and estradiol (E₂) predicted similar, but slightly smaller sizes at maturity than the morphological criterion. Stage of maturity for males was estimated identically using internal organs or external secondary sexual characters, thus allowing non-lethal maturity assessments. Size at median maturity was greater north of Point Conception for females, and north of Cape Mendocino for males. Peak parturition occurred from May to October, with increased concentrations of E₂ in skeletal muscle of females correlating with ovarian recrudescence during November to February. No significant seasonal trends in female T were apparent, but mean female 11-ketotestosterone (11KT) was 300% greater in April than any other month during the parturition season. There was a marginal evidence for increased number and size of ova with maternal size. Extrapolation of the hypothesized 6 to 8-month egg-laying season to observed mean parturition rates of captive specimens yielded an estimated annual fecundity of 19.5–28.9 egg cases. Differences in fecundity among higher taxonomic classifications of chondrichthyans were detected with chimaeriform fishes more fecund than lamniform, myliobatiform, squaliform, and rhinobatiform fishes, and less fecund than rajiform fishes. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Relatedness,recognition errors,and colony fusion in the termite <Emphasis Type="Italic">Nasutitermes corniger</Emphasis>

Loss of aggression between social groups can have far-reaching effects on the structure of societies and populations. We tested whether variation in the genetic structure of colonies of the termite Nasutitermes corniger affects the probability of aggression toward non-nestmates and the ability of unrelated colonies to fuse. We determined the genotypes of workers and soldiers from 120 colonies at seven polymorphic microsatellite loci. Twenty-seven colonies contained offspring of multiple founding queens or kings, yielding an average within-colony relatedness of 0.33. Genotypes in the remaining 93 colonies were consistent with reproduction by a single queen and king or their progeny, with an average within-colony relatedness of 0.51. In standardized assays, the probability of aggression between workers and soldiers from different colonies was an increasing function of within-colony relatedness. The probability of aggression was not affected significantly by the degree of relatedness between colonies, which was near zero in all cases, or by whether the colonies were neighbors. To test whether these assays of aggression predict the potential for colony fusion in the field, we transplanted selected nests to new locations. Workers and soldiers from colonies that were mutually tolerant in laboratory assays joined their nests without fighting, but workers and soldiers that were mutually aggressive in the assays initiated massive battles. These results suggest that the presence of multiple unrelated queens or kings promotes recognition errors, which can lead to the formation of more complex colony structures.     

Spawning patterns in the leopard grouper, <Emphasis Type="Italic">Mycteroperca rosacea</Emphasis>, in comparison with other aggregating groupers

We documented the spawning patterns of the leopard grouper, Mycteroperca rosacea, from April to June 2005 in the central Gulf of California, Mexico to draw comparisons with other aggregate-spawning groupers and provide information useful for management of their fishery. Adults formed spawning aggregations of 150 to >700 individuals at specific sites, and spawning occurred daily at these sites from late April through early June. Courtship occurred throughout the day, but spawning was restricted to the evening hours. Adults spawned in groups of 6–40 fish, and pair-spawning was not observed. The group-spawning behavior of adults and the gonosomatic indices of mature males (maximum = 7.2%) suggest that sperm competition was present. The site-specificity of leopard grouper spawning aggregations and diel spawning period were typical of most aggregating groupers, and the size and structure of these aggregations was similar to other species in the genus Mycteroperca. Leopard grouper behavior patterns were unusual in that spawning aggregations persisted for extended periods, spawning was not synchronized with the lunar cycle, and adults aggregated during non-spawning periods. The extensive duration and site-specificity of spawning aggregations and the propensity of M. rosacea to form aggregations year-round increases the vulnerability of the species to overfishing. Policies that limit harvest from these aggregations are needed to improve the management of leopard grouper fisheries in the Gulf of California.     

Disease resistance in a weaver ant, <Emphasis Type="BoldItalic">Polyrhachis dives</Emphasis>, and the role of antibiotic-producing glands

Parasites represent one of the main threats to all organisms and are likely to be particularly significant for social animals because of the increased potential for intragroup transmission. Social animals must therefore have effective resistance mechanisms against parasites and one of the most important components of disease resistance in ants is thought to be the antibiotic-producing metapleural gland. This gland is ancestral in ants, but has been lost secondarily in a small number of species. It is unknown whether these evolutionary losses are due to a reduction in parasite pressure or the replacement of the gland’s function with other resistance mechanisms. Here we used the generalist entomopathogenic fungus Metarhizium to compare the disease resistance of a species of a weaver ant, Polyrhachis dives, which has lost the metapleural gland, with that of the well-studied leaf-cutting ant Acromyrmex echinatior and two other ant species, Myrmica ruginodis and Formica fusca, all of which have metapleural glands. The P. dives weaver ants had intermediate resistance when kept individually, and similar resistance to A. echinatior leaf-cutting ants when kept in groups, suggesting that the loss of the metapleural gland has not resulted in weaver ants having reduced disease resistance. P. dives weaver ants self-groomed at a significantly higher rate than the other ants examined and apparently use their venom for resistance, as they had reduced resistance when their venom gland was blocked and the venom was shown in vitro to prevent the germination of fungal spores. Unexpectedly, the leaf-cutting ant A. echinatior also had reduced resistance to Metarhizium when its venom gland was blocked. It therefore appears that the evolutionary loss of the metapleural gland does not result in reduced disease resistance in P. dives weaver ants, and that this at least in part may be due to the ants having antimicrobial venom and high self-grooming rates. The results therefore emphasise the importance of multiple, complementary mechanisms in the disease resistance of ant societies.     

Energy allocation and metabolic scope in early turbot, <Emphasis Type="Italic">Scophthalmus maximus</Emphasis>, larvae

Early stages of marine fish larvae are characterized by fast growth while having a limited aerobic scope and an immature digestive system. In order to understand this apparent paradox, the study of energy allocation is a major necessity. Components of the energy budget of turbot (Scophthalmus maximus) larvae were determined during initial development (days 4–12) and the complete energy allocation budget is presented. It was observed that food absorption efficiency increased from 32 to 51% during the studied period, and so did the energy available for growth and metabolic purposes. The relative amount of energy for maintenance decreased from 71 to 36% of energy channelled to metabolism. Gross growth efficiency increased from 20 to 26% of ingested energy, and net growth efficiency decreased from 66 to 52% of assimilated energy. Reduction of net growth efficiency is the reflex of a higher metabolic rate in older larvae, due to increased costs of activity and growth. Evidence, indicating that metabolic scope of early turbot larva is unable to accommodate simultaneously high levels of growth and activity was found. Alternative strategies to accommodate the costs of growth and activity exist in turbot larvae, and may result in a trade-off between fast growth and viability. As larvae grow, the various physiological processes described get more efficient, and the metabolic scope increases.     

Increased sperm numbers in the vas deferens of meadow voles, <Emphasis Type="Italic">Microtus pennsylvanicus,</Emphasis> in response to odors of conspecific males

Sperm competition occurs when the sperm of two or more males compete to fertilize the egg/s of a particular female. Males of some species respond to a high risk of sperm competition by increasing the number of sperm in their ejaculates. Males may accomplish such a response by increasing the intensity or duration of contraction of the cauda epididymidis and vas deferens. During emission (first phase of the ejaculatory process), the vas deferens receives sperm from the cauda epididymidis and propels the sperm to the urethra. In this paper, we tested the hypothesis that males exposed to a high risk of sperm competition mobilize larger numbers of sperm from the cauda epididymidis to the vas deferens before initiation of copulatory behavior. This accumulation of sperm in the vas deferens would result in a larger number of sperm in the ejaculate. To test this hypothesis, we exposed male meadow voles, Microtus pennsylvanicus, to either low or high risks of sperm competition using soiled bedding of conspecific individuals. At three different times after this exposure (15, 30, or 60 min), we removed both vasa deferentia and counted the sperm within them. We found a significant increase in sperm numbers in the vas deferens of males after 30 min of being exposed to a high risk of sperm competition. The lower sperm numbers after 15 and 60 min of exposure suggest that the observed response is relatively slow and that sperm mobilized to the vasa deferentia may return to the cauda epididymides if ejaculation does not occur some time after the observed response. Our results indicate that the physiological response that may result in high sperm numbers in the ejaculate in relation to high risk of sperm competition can occur before initiation of copulatory behavior.     

Physiological stress responses in the edible crab, <Emphasis Type="Italic">Cancer pagurus</Emphasis>, to the fishery practice of de-clawing

We examined physiological stress responses in the edible crab, Cancer pagurus, subjected to the commercial fishery practice of manual de-clawing. We measured haemolymph glucose and lactate, plus muscular glycogen and glycogen mobilisation, in three experiments where the crabs had one claw removed. In the first, crabs showed physiological stress responses when ‘de-clawed’ as compared to ‘handled only’ over the short term of 1–10 min. In the second, de-clawing and the presence of a conspecific both increased the physiological stress responses over the longer term of 24 h. In the third, de-clawing was shown to be more stressful than ‘induced autotomy’ of claws. Further, the former practice caused larger wounds to the body and significantly higher mortality than the latter. Since the fishery practice is to remove both claws, the stress response observed and mortality data reported are conservative.     

Pair formation,pre-spawning waiting,and protandry in kokanee,<Emphasis Type="Italic"> Oncorhynchus nerka</Emphasis>

The timing of arrival to breeding areas can have profound effects on reproductive success. Under some conditions (restricted breeding seasons and mating systems characterized by a longer period of mating among males than females), the maximization of mating opportunities by males theoretically selects for the earlier arrival of males than females (a phenomenon called protandry). This study quantifies the relationship between the arrival timing and spawning success of male kokanee (non-anadromous Oncorhynchus nerka). The spawning behavior of kokanee was observed in a large pen and the spawning success of each male was estimated as the number of spawning events he participated in. A male's spawning success depended primarily on his success at pairing with and mate-guarding females, and less on participation in spawning events while unpaired. Males who paired earlier in the season had higher spawning success than males who paired later in the season because they experienced more opportunities to pair with new females. Among males who eventually paired (some males never did), arriving early was correlated with pairing early. However, selection for protandry was weak, largely because early arrival did not guarantee that a male would pair. Pre-spawning waiting by females also weakened the correlation between arrival day and pairing day. The random probability of pairing with respect to arrival day and pre-spawning waiting by females likely explains the weak selection for protandry in kokanee and the low amount of protandry observed in other sockeye salmon (anadromous O. nerka) populations.     

Larval ecology of the great barracuda, <Emphasis Type="Italic">Sphyraena barracuda</Emphasis>, and other sphyraenids in the Straits of Florida

The great barracuda (Sphyraena barracuda) is a widespread, ecologically and socioeconomically important coastal fish, yet very little is known about its larvae. We examined spawning and larval ecology of Western Atlantic sphyraenids using monthly ichthyoplankton samples collected over 2 years along a transect spanning the east–west axis of the Straits of Florida (SOF). Samples were dominated by the great barracuda (92.8%) and sennets (Sphyraena borealis and Sphyraena picudilla; 6.6%). While larval sennets and S. barracuda displayed similar vertical distributions (majority in upper 25 m), horizontal and temporal patterns of abundance suggested a spatial and temporal species replacement between larval S. barracuda and sennets that tracks adult ecology. The diet of both taxa consisted largely of copepods, with inclusion of fish larvae at 8 mm SL, and in S. barracuda alone, a switch in the wet season to exclusive piscivory by 12 mm SL (18 days post-hatch). A lack of piscivory in S. barracuda larvae captured in the dry season corresponded to slower larval growth than in the wet season. Larval growth was also related to size-at-hatch and larval age such that larvae that were larger at hatch or larger (older) at capture grew faster at earlier ages, suggesting faster larval growth, and indirectly larger hatch size, conveys a survival advantage. Unlike larval growth, instantaneous mortality rate did not differ with season, and no lunar cyclic patterns in spawning output were identified. Our results provide insight into the pelagic phase of sphyraenids and highlight the importance of both diet and hatch size to the growth and survival of fish larvae in low latitude oceanic environments.     

Reproductive biology of the spiny lobster, <Emphasis Type="Italic">Panulirus Penicillatus</Emphasis>, in the southeastern coastal waters off Taiwan

The reproductive biology of spiny lobster, Panulirus penicillatus, was studied based on 2,068 lobsters, ranging from 34.28 to 131.60 mm carapace length (CL), sampled in Taitung coastal waters from September 2003 to December 2004. The overall sex ratio approximated 1:1 (χ² = 0.02, P > 0.05), but the monthly sex ratios in 2004 showed significant differences and males were predominant in sizes larger than 80 mm CL. Reproductive activity, assessed using histology, a gonadosomatic index and percentage of ovigerous females, indicated that the mature females could be found in every month and that the major spawning occurred from May to September. The presence of re-developing/re-ripe ovaries by month and size-specific spawning time suggest that larger mature females (>60 mm CL) spawn at least three times a year while smaller new mature females spawn at least once a year. For females, the estimated sizes at 50% physiological and functional maturity were (mean ± SE) 56.46 ± 0.56 mm CL and 66.63 ± 1.07 mm CL. The estimated sizes at functional maturity were between 72 and 74 mm CL for males. The number of eggs per spawning event (brood size, BS) was related to CL by the equation Y _BS = 2.4 × 10^-3CL^4.18 (r ² = 0.902, n = 12). Female lobsters with CL ranging from 60 to 80 mm made the greatest contributions to egg production because of their high brood size and active reproductive activity. A minimum legal size should be established for the fishery to protect egg production potential of lobster population in the southeastern coastal waters off Taiwan.     

Loss of Forest Cover in Kalimantan, Indonesia, Since the 1997–1998 El Niño

Abstract:  Deforestation in Indonesia poses a significant threat to the region's biodiversity. We mapped forest cover in Kalimantan, Indonesia, in 2002, with imagery provided by the Moderate Resolution Imaging Spectrometer (MODIS). The MODIS-based forest and nonforest map showed good agreement with other sources of recent data on forest cover. Comparison of MODIS forest cover with Indonesian government data from 1996 revealed that almost 3 million ha of forest were lost in Kalimantan since the major El Niño event of 1997–1998, when a drought produced unprecedented biomass burning in the region. Over two-thirds of the deforestation occurred in proposed and existing protected areas, especially those of 100,000–250,000 ha. The loss of forest in proposed and existing protected areas suggests that Kalimantan's protected-area network is no longer viable and that alternative conservation strategies, such as timber certification and improved monitoring and enforcement, are needed to preserve remaining forest habitats there.