Physiological responses of Avicennia marina seedlings to the phytotoxic effects of the water-soluble fraction of light Arabian crude oil

Stomatal behavior, growth performance and the accumulation of polynuclear aromatic hydrocarbons (PAHs) were evaluated in seedlings of the mangrove Avicennia marina (Forssk.) Vierh., treated with a water-soluble fraction (WSF) of Abu-Dhabi light Arabian crude oil through foliar spraying or soil application.Irregular stomatal behavior and weak stomatal control over transpiration were observed during the first 24 hours, where stomatal resistances of plants sprayed with 150 and 300 g PAHs plant^–1 were significantly lower than that of the control plants. After six weeks, all treated plants showed no significant difference in their relative growth rate (RGR) or in the net assimilation rate (NAR) compared with the control plants.Tri-aromatic hydrocarbons were the most accumulated in tissues of the treated plants. Penta- and hexa-aromatics, on the other hand, were undetectable in the WSF and consequently in the treated plants. A linear relationship was observed between the dose applied to plants and the amounts of tissue accumulated PAHs (r ²=0.515 for soil application and r ²=0.984 for foliar spray). In plants sprayed with 300 g PAHs plant^–1, the total PAHs accumulated were more than that accumulated in plants treated through soil application.These findings suggest that: aqueous extraction of crude oil tends to signify the percentage of the low molecular weight PAHs, e.g. naphthalene, to the total PAHs; disturbed stomatal behavior in the first day of the treatment may be due to the venting of the volatile low molecular weight aromatic hydrocarbons (e.g. benzene, toluene, and xylenes) through the stomata; and uptake of water-soluble hydrocarbons by plants is equally possible through both of the root system and the foliage. The ecological implications of these finding are discussed in relation to oil pollution of mangrove stands under field conditions.     

Crisis Water Management and Ibis Breeding at Narran Lakes in Arid Australia

Narran Lakes is a Ramsar site recognised for its importance for colonial waterbird breeding, which only occurs after large highly variable flooding events. In 2008, 74,095 pairs of ibis bred for the first time in seven years, establishing two contiguous colonies, a month apart. Most (97%) of the colony consisted of the straw-necked ibis (Threskiornis spinicollis) with the remainder consisting of glossy ibis (2%, Plegadis falcinellus) and Australian white ibis (1%, T. molucca). Following cessation of river flows, water levels fell rapidly in the colony site, resulting in a crisis management decision by governments to purchase and deliver water (10,423 Ml) to avert mass desertion of the colonies. There were significant differences in the reproductive success of each colony. In colony 1 60% of eggs hatched and 94% of chicks fledged, while in colony 2 40% of eggs hatched with only 17% of chicks fledging. Statistical analyses found that water depth was a significant variable in determining reproductive success. Rapid falls in water level during the chick stage in colony 2 resulted in decreased chick and overall offspring success. The results of this study identify the impact of upstream water resource development on colonial waterbird breeding and have implications for water management policies.     

Modeling the Relations Between Flow Regime Components,Species Traits,and Spawning Success of Fishes in Warmwater Streams

Modifications to stream hydrologic regimes can have a profound influence on the dynamics of their fish populations. Using hierarchical linear models, we examined the relations between flow regime and young-of-year fish density using fish sampling and discharge data from three different warmwater streams in Illinois, Alabama, and Georgia. We used an information theoretic approach to evaluate the relative support for models describing hypothesized influences of five flow regime components representing: short-term high and low flows; short-term flow stability; and long-term mean flows and flow stability on fish reproductive success during fish spawning and rearing periods. We also evaluated the influence of ten fish species traits on fish reproductive success. Species traits included spawning duration, reproductive strategy, egg incubation rate, swimming locomotion morphology, general habitat preference, and food habits. Model selection results indicated that young-of-year fish density was positively related to short-term high flows during the spawning period and negatively related to flow variability during the rearing period. However, the effect of the flow regime components varied substantially among species, but was related to species traits. The effect of short-term high flows on the reproductive success was lower for species that broadcast their eggs during spawning. Species with cruiser swimming locomotion morphologies (e.g., Micropterus) also were more vulnerable to variable flows during the rearing period. Our models provide insight into the conditions and timing of flows that influence the reproductive success of warmwater stream fishes and may guide decisions related to stream regulation and management.     

PHREATOPHYTE EVAPOTRANSPIRATION AND ITS POTENTIAL REDUCTION WITHOUT ERADICATION1

ABSTRACT: The continuous availability of ground water to riparian phreatophytic vegetation results in large evapotranspiration (ET) losses in summer. Chemical or physical eradication of this vegetation can have undesirable environmental side effects. Spraying phreatophyte foliage with a nontoxic antitranspirant (AT) may reduce transpiration without eradication. Transpiration rate per unit leaf area was similar for several phreatophyte species, but ET per unit land area of phreatophytes depneds more on stand density than species. The mean ET for saltcedar in June was 8.1 mm/day measured by Bowen ratio, compared with 7.9 mm by lysimeters. ATs and growth-retardants reduced transpiration by over 50 percent in laboratory tests where foliage was thoroughly sprayed. In the field AT sprayed by a back-pack mistblower reduced ET by 20–35 percent initially and 10 percent after a month. No ET reduction occurred when AT was sprayed by helicopter on saltcedar, because excessive droplet size and heavy salt deposits on the foliage resulted in poor spray adherence. Wax-based AT was relatively nontoxic to fish and wildlife. Dissolved oxygen could be reduced for aquatic life, but further AT dilution in streams and ponds would minimize this. Helicopter spraying may affect bird nests and egg hatchability. Although ATs significantly reduce ET, their high cost and spraying difficulties preclude current use on phreatophytes. With improvement they may economically help to conserve water in riparian areas in future years.     

EFFECTS OF TREATED BLEACHED KRAFT MILL EFFLUENT ON EGGS AND PROLARVAE OF STRIPED BASS (MORONE SAXATILIS)1

ABSTRACT: Striped bass (Morone saxatilis) eggs and prolarvae were exposed to a range of treated bleached kraft mill effluent (BKME) concentrations from 0 to 20 percent effluent by volume (v/v) under continuous flow test conditions. The treated BKME used in the study before dilution had a BOD₅ of 18 to 25 mg/l, TSS of 60 to 185 mg/l and true color of 1750 to 1755 mg/l. No mortality attributable to BKME was found in three separate egg studies. No delayed hatching of the eggs occurred in any of the BKME treatments relative to the control treatment. No significant morphological abnormalities (pugheadness, truncation, or scoliosis) were found in prolarvae exposed to BKME from the egg stage through six days post/hatch. Tests with prolarvae revealed that BKME at concentrations from 2 to 20 percent v/v did not cause mortality during exposures up to 60 hours. However, BKME concentrations ranging between 8 to 12 percent up to 20 percent v/v caused increased mortality after 72 hours of exposure.     

Contaminant Exposure and Effects in Red-Winged Blackbirds Inhabiting Stormwater Retention Ponds

Stormwater wetlands are created to retain water from storms and snow melt to reduce sediment, nutrient, and contaminant pollution of natural waterways in metropolitan areas. However, they are often a source of attractive habitat to wetland-associated wildlife. In this study of 12 stormwater wetlands and a larger, older reference site, elevated concentrations of zinc and copper were found in sediments and carcasses of 8-day-old red-winged blackbird (Agelaius phoeniceus) nestlings inhabiting stormwater sites. Although nesting success in the stormwater wetlands was comparable to national averages, sediment zinc concentrations correlated with clutch size, hatching success, fledgling success, and Mayfield nest success, suggesting that the nestlings may have been stressed and impaired by elevated zinc. This stress may have been direct on the nestlings or indirect through effects on the availability of food organisms.Published online     

Oil spill studies: A review of ecological effects

We reviewed seven particularly well known and/or studied oil spills that have occurred since the National Academy of Sciences 1975 report, Petroleum in the Marine Environment or that occurred prior to that report but about which significant new information has since been acquired. The spills studied were from the bargeFlorida, and tankersArrow, Argo Merchant, Amoco Cadiz, andTsesis and blowouts from the Bravo and Ixtoc I platforms.These best studies yield only limited insight into effects because they lack controls and have a high degree of natural variability. TheTsesis, Florida, andAmoco Cadiz cases are exceptional since they occurred in areas of ongoing research programs and had nearby areas suitable for controls. Oil spills have produced measurable effects on ecosystems that have not been readily predictable from laboratory studies on isolated organisms. However, ecosystemlevel interactions are poorly understood even without the complications resulting from effects of pollution. These generalizations emerge: oil regularly reaches sediments after a spill; oil in anoxic sediments is persistent; oil regularly contaminates Zooplankton and benthic invertebrates; fish are also contaminated, but to a lesser extent; oil contamination decreases the abundance and diversity of benthic communities.     

Sequential bioconversion of used paper to sugars by cellulases from Trichoderma reesei and Penicillium funiculosum

Used paper, a potential resource of alternative energy, can be recycled but mostly it forms a significant component of solid waste. Used office paper, foolscap paper, filter paper and newspaper have been treated with cellulase from Trichoderma reesei and Penicillium funiculosum to bioconvert their cellulose component into sugars. Both non-pretreated and pretreated paper was incubated successively with the two cellulases during four consecutive incubation periods of 1 h each. The amount of sugars released during this sequential treatment was compared with the total sugar produced during a 4 h period of continuous incubation with each enzyme system independently. Pre-treatment milling of paper proved to effectively increase the sugar formation under all incubation conditions. Successive incubation with the two enzyme systems of both non-pretreated and pretreated paper materials was more efficient than the corresponding continuous bioconversion. The highest relative sugar yield was experienced during successive treatment of pretreated materials when T. reesei cellulase initiated the degradation. However, maximum bioconversion of pretreated newspaper was obtained when P. funiculosum initiated degradation. Pretreated foolscap paper was the most susceptible substrate with maximum bioconversion when exposed to both forms of successive cellulase treatment.     

Fish Species Used as Biomarker for Heavy Metal and Hydrocarbon Contamination for Cross River, Nigeria

Enhanced concentrations of Fe, Zn, Mn, Pb, Cu, Cd and total hydrocarbons (THC) determined in fish samples from the Cross River system, Nigeria have been associated with clinical defects (nausea, headache, hepatitis, body rashes) observed in coastal residents who are the major consumers of the fish species. Fish samples were collected from ten locations with varying degrees of exposure to human activities. Heavy metal concentrations in fish followed the sequence: Fe>Zn>Mn>Pb>Cu>Cd with the highest concentration of 243 g/g (Fe) wet weight occurring in Tympanotonus sp. Fe levels were significantly (P<0.001) higher than other metals analysed. The sequence in total hydrocarbon concentrations according to fish species was in the order of O. niloticus (55.1 g/g) > E. fimbriata > P. elongatus > Portonus sp. > C. nigrodigitatus > Tympanotonus sp. Generally, the demersal species showed a marked potential for tolerating high levels of heavy metals while the pelagic species showed preference for the accumulation of hydrocarbons. The degree of contamination depended on pollutant type, fish species, sampling location, trophic level and their mode of feeding. The persistent accumulation and tolerable potential of Tympanotonus, Portonus and P. elongatus suggest that they might be effectively utilized as self-integrating indicators for time-series monitoring of the rate of recovery of the impacted ecosystem by heavy metals. Possible sources of pollutant include leachates from municipal dumps, used crankcase oils from fluvial discharges (mechanic workshops) and occasional oil spills.     

SOME EFFECTS OF ACIDIFIED WATER ON THE EARLY DEVELOPMENT OF ROACH (Rutilus rutilus L.) AND PERCH (Perca fluviatilis L.)1

ABSTRACT. Fertilized eggs of perch (Perca fluviatilus L.) and roach (Rutilus rutilus L.) were reared at different pH values both in the field and in the laboratory. The results from the laboratory tests revealed that both species showed at least a 50% decrease in their hatching frequencies when reared at pH values below 5.6. At pH values below 4.6 both species studied show an almost complete lack of reproduction. The field studies with eggs from different lakes indicated that there might be an adaptation, manifesting itself as a higher frequency of hatching in water with a composition similar to that of the natural habitat. Field experiments also showed that the roach is more sensitive than perch to low pH values.     

Impact of Fencing on the Recovery of the Ground Flora on Heavily Eroded Slopes of a Deciduous Forest

This paper seeks to outline early stages in the recovery of forest ground flora on eroded slopes impacted by recreation activities and to suggest how these data might be applied in the formulation of management policies for forest recreation areas. Based on a fencing experiment in the Sonian Forest near Brussels, we investigated whether, over a 6-year period, the vegetation was able to recover after having been destroyed by recreation use. Short-term trends in overall species composition were already observable during this 6-year study. Species recovery on eroded hills was related to slope, aspect, and soil type. During the considered time scale, the proportion of hemicryptophytes and the number of ancient forest species increased significantly. A downward trend was detected for Ellenbergs nitrogen and temperature indexes and for the proportion of therophytes and pioneer plants of disturbed places. Changes in species frequencies suggest six recovery strategies: early, late, expanding, disappearing, transient, and fluctuating species. Aside from seedling reproduction from overstory influences, Luzula sylvatica appeared to be the most resilient of the species identified in the study since this species has the highest global frequency in our sampling plots and has increased its cover during the study period. Study results indicate that (1) protection from recreation has initiated the recovery of species in the herb layer, but (2) it may take a long time before vegetation previously present in the ground flora may recover in both density and species composition.     

EFFECTS OF NITRATE AND PHOSPHATE CONCENTRATION ON NATURAL AQUATIC BACTERIAL POPULATIONS1

ABSTRACT: Natural aquatic bacterial populations in three streams located at the Savannah River Project, Aiken, S.C. have been studied in relation to the effects of ambient temperatures, dissolved River Project, Aiken, oxygen (DO), nitrate and phosphate concentrations. ³ 3 This work was supported in part by Contract #AT (38–1–824) with the U.S. Atomic Energy Commission, and was done in part in connection with AEC Contract #AT (07–2)–1.
Samples collected at monthly intervals for a period of one year from each system, were plated in duplicate at each of two dilutions on 1/4 strength Standard Plate Count Agar (Difco). After incubation at 25±1 °C for four days, total colony forming units, percent chromagens, and number of colony types (diversity) were determined and colonies were picked for identification. Temperatures were generally equal in two of the streams throughout the year, being lowest in Upper Three Runs (U3R) and Tirns Branch (TB), and highest in the Ash Basin System (ABS). DO content did not vary appreciably between the streams. Nitrates and phosphates were lowest in U3R, next lowest in TB, higher in the last station in ABS, and highest in the ash basin per se. Total colony forming units were highest in the ash basin, whereas chromagen percentage and diversity were highest in the last station in ABS. Results of these studies indicate that high nitrates and phosphates, in the absence of high organic carbon concentrations, have little, if any detrimental effect on the stability of natural aquatic bacterial populations.     

Managing Tree-of-Heaven (Ailanthus altissima) in Parks and Protected Areas: A Case Study of Rondeau Provincial Park (Ontario, Canada)

The Carolinian Life Zone in southwestern Ontario, Canada is valued because it represents an almost disjunct ecosystem (i.e., one that is typical of the mid-Atlantic United States, rather than the rest of Canada or the nearby states in the United States). The landscape of the Carolinian Life Zone has undergone dramatic transformation, especially in recent decades as agriculture, urbanization, and recreation have intensified. One of the most apparent changes is the invasion of exotic plant species that exacerbates the need for mass restoration efforts. Within the Carolinian Life Zone, Rondeau Provincial Park has experienced an influx of nonindigenous, invasive species in recent years. Tree-of-heaven (Ailanthus altissima) is one example. The infestation is still relatively localized to (mainly) the park, slowly spreading, and manageable as long as something is done immediately. We examined the effects of hand-pulling and mulching, cut stump and glyphosate application, cut stump alone, and the EZJect Capsule Injection System (using glyphosate) on the management of A. altissima within the park. Cut stump and glyphosate treatment was most effective and efficient in its control of young A. altissima shoots because it limits disturbance and has acceptable capital and operating costs. Hand-pulling and mulching was the second choice, mainly because of the risk of additional disturbance that increased shoot densities 1 year after treatment. Cut stump alone was not effective, worsened the infestation, and is not recommended for this species. The EZJect system was effective at managing mature, seed-producing shoots, although the somewhat higher capital costs mean that the system probably should be purchased for management of several invasive tree species to make it more cost-effective.     

Effects of Excess Copper on Growth of Wild Rice (<Emphasis Type="Italic">Zizania palustris</Emphasis>) Seedlings Tested in Reconstituted and Natural Waters

Dissolved copper was toxic to wild rice (Zizania palustris) seedlings when exposed in water from the seed collection site of Swamp Creek, Crandon, Wisconsin, USA, and in laboratory-prepared artificial or reconstituted water. Seeds for the study were harvested, then held through a portion of dormancy, in Swamp Creek. After 60 days they were shipped to a laboratory, chilled, and tested with copper after germination. The end point of the tests was net gain in wet weight of the seedlings; additionally, a pronounced reduction in root development was observed. Using measured concentrations, the lowest no-observable-effect concentration (NOEC) in our study was 37 g/liter in Swamp Creek water and the lowest-observable-effect concentration (LOEC) was 59 g/liter. However, it appeared that there was a point at which concentrations of copper above 400 g/liter did not result in any measurable effect or exhibit a definitive dose–response. Because the results in Swamp Creek water were more relevant to the possibility of local metals additions and the association of reduced seedling growth by copper was more powerful in this water, we derived an equation to express the relationship between copper concentration and toxicity for Swamp Creek water. As an example, we would expect a 3.0% reduction in seedling growth at 5.0 g/liter copper. Seedling roots were particularly affected and the resultant plants may be less well anchored and more susceptible to dislodging than plants not exposed to copper. Further refinement of the methodology may be used to address effects of other contaminants impacting rice beds in North America. This study was conducted while the first author was employed by the U.S. Geological Survey, Mid-continent Ecological Science Center, Fort Collins, Colorado     

Pulpwood,pesticides, and people. Controlling spruce budworm in northeastern North America

The eastern spruce budworm is a major forest pest over the continental range of the spruce-fir forest ecosystem and its southern ecotonal fringes in Canada and the northeastern United States. The current budworm outbreak illustrates the difficulty of arriving at economically sound and publicly acceptable forest pest control policies. Policies ranging from no use of chemical control to annual widespread crop protection have been adopted. There is no single all-around best policy for spruce budworm control. Chemical spray programs have demonstrably slowed the normal progress of mortality due to budworm, but have not eradicated the pest. Where industry remains heavily dependent on a fully utilized spruce-fir forest, no easy, low-cost solutions to the budworm problem exist. Reliance on spraying will have to be reduced and plans made to utilize higher levels of tree mortality and to manage the forest for lower future vulnerability.     

Erosional Consequence of Saltcedar Control

Removal of nonnative riparian trees is accelerating to conserve water and improve habitat for native species. Widespread control of dominant species, however, can lead to unintended erosion. Helicopter herbicide application in 2003 along a 12-km reach of the Rio Puerco, New Mexico, eliminated the target invasive species saltcedar (Tamarix spp.), which dominated the floodplain, as well as the native species sandbar willow (Salix exigua Nuttall), which occurred as a fringe along the channel. Herbicide application initiated a natural experiment testing the importance of riparian vegetation for bank stability along this data-rich river. A flood three years later eroded about 680,000 m³ of sediment, increasing mean channel width of the sprayed reach by 84%. Erosion upstream and downstream from the sprayed reach during this flood was inconsequential. Sand eroded from channel banks was transported an average of 5 km downstream and deposited on the floodplain and channel bed. Although vegetation was killed across the floodplain in the sprayed reach, erosion was almost entirely confined to the channel banks. The absence of dense, flexible woody stems on the banks reduced drag on the flow, leading to high shear stress at the toe of the banks, fluvial erosion, bank undercutting, and mass failure. The potential for increased erosion must be included in consideration of phreatophyte control projects.     

Temperatures and Locations Used by Hibernating Bats,Including <Emphasis Type="Italic">Myotis sodalis</Emphasis> (Indiana Bat), in a Limestone Mine: Implications for Conservation and Management

Understanding temperatures used by hibernating bats will aid conservation and management efforts for many species. A limestone mine with 71 km of passages, used as a hibernaculum by approximately 30,000 bats, was visited four times during a 6-year period. The mine had been surveyed and mapped; therefore, bats could be precisely located and temperatures (T _s) of the entire hibernaculum ceiling accurately mapped. It was predicted that bats should hibernate between 5 and 10°C to (1) use temperatures that allow a near minimal metabolic rate, (2) maximize the duration of hibernation bouts, (3) avoid more frequent and prolonged arousal at higher temperatures, (4) avoid cold and freezing temperatures that require an increase in metabolism and a decrease in duration of hibernation bouts or that could cause death, and (5) balance benefits of a reduced metabolic rate and costs of metabolic depression. The distribution of each species was not random for location (P < 0.000) or T _s (P < 0.000). Myotis sodalis (Indiana bat) was most restricted in areas occupied, hibernating in thermally stable yet cold areas ( = 8.4 ± 1.7°C); 99% associated with cement block walls and sheltered alcoves, which perhaps dampened air movement and temperature fluctuations. Myotis lucifugus (little brown myotis) hibernated in colder, more variable areas ( = 7.2 ± 2.6°C). Myotis septentrionalis (northern myotis), Pipistrellus subflavus (eastern pipistrelle), and Eptesicus fuscus (big brown bat) typically hibernated in warm, thermally stable areas ( = 9.1 ± 0.2°C, = 9.6 ± 1.9°C, and = 9.5 ± 1.5°C, respectively). These data do not indicate that hibernacula for M. sodalis, an endangered species, should be manipulated to cool below 5°C.     

What's wrong with animalby-products?

Without looking beyond the conditions under which laying hens typically live in the contemporary U.S. egg industry, we can understand why the production and consumption of factory farmed eggs could be judged immoral. However, the question, What (if anything) is wrong with animal by-products? cannot always be adequately answered by looking at the conditions under which animals live out their productive lives. For the dairy industry looks benign in those terms, but if we look beyond the conditions under which milk cows live, we can better understand some animal rights activists' reasons for objecting to dairy products. The contemporary U.S. dairy industry requires a slaughter industry between one-seventh and one-third the size of the contemporary beef industry. Today, beef slaughter is vastly more humane than poultry slaughter, but if today's beef slaughter industry is judged emmoral, the contemporary dairy industry should be judged similarly immoral, because the two are wedded. This is the deep reason for moral suspicion of the dairy industry.     

Sludge-grown algae for culturing aquatic organisms: Part I. Algal growth in sludge extracts

This project is aimed at studying the feasibility of using sewage sludge to prepare culture media for microalgae (Chlorella-HKBU) and the use of the sludge-grown algae as a feed for some aquatic organisms. Part I of the project included results on preparing sludge extracts and their use on algal culture. By comparing two culturing techniques, aeration and shaking, it was noted that both lag and log phases were shortened in the aeration system. A subsequent experiment noted that algal growth subject to aeration rates of 1.0 and 1.5 liters/min had similar lag and log phases. In addition, both aeration rates had a significantly higher (P < 0.05) final cell density than that of 0.5 liters/min. A detailed study on the variation of growth conditions on the algal growth was done. The results indicated that pH values of all the cultures declined below 5 at day 12. The removal rates of ammonia N ranged from 62% to 70%. The sludge-grown algae contained a rather substantial amount of heavy metals (µg/g): Zn 289–581, Cu 443–682, Ni 310–963, Mn 96–126, Cr 25–118, and Fe 438–653. This implied that the rather high levels of heavy metals may impose adverse effects on higher trophic organisms.     

Foraging areas of king penguins from Macquarie Island in relation to a marine protected area

Twenty-three king penguins (Aptenodytes patagonicus) from Macquarie Island were tracked by satellite during the late incubation period in 1998–1999 to determine the overlap of the foraging zone of king penguins with an area to be declared a marine protected area (MPA) near the island. While all penguins left the colony in an easterly direction and traveled clockwise back to the island, three penguins foraged in the northern parts of the general foraging area and stayed north of 56°S. The remaining 20 penguins ventured south and most crossed 59°S before returning to the island. The total foraging area was estimated to be 156,000 km² with 36,500 km² being most important (where penguins spent >150 hr in total). North-foraging penguins reached on average 331 ± 24 km from the colony compared to 530 ± 76 km for the south-foraging penguins. The latter traveled an average total distance of 1313 ± 176 km, while the northern foragers averaged 963 ± 166 km. Not only did the penguins spend the majority of their foraging time within the boundaries of the proposed MPA, they also foraged chiefly within the boundaries of a highly protected zone. Thus, the MPA is likely to encompass the foraging zone of king penguins, at least during incubation.