Functional-ecological role of biological diversity in populations and communities

The functional role of differentiation with respect to local population density, expression of responses to the presence of other individuals, and body weight has been analyzed from an ecoenergetic standpoint with consideration of known mathematical laws. The results indicate that increased variation in these parameters (i.e., their higher diversity) improves the efficiency of energy flow through the animal population: the input of energy increases, while its expenditures decrease. The greatest effect is achieved when the population is divided into alternative modal groups.     

The internal exposure of Taiwanese to phthalate--an evidence of intensive use of plastic materials

Phthalates are widely used in industry and consumer products. Di-(2-ethylhexyl) phthalate (DEHP) and di-n-butylphthalate (DBP) show the greatest potency of reproductive toxicants among phthalates. The purposes of this study are to examine the migration level of phthalate from PVC films by simulating food handling and to reveal the body burden of phthalate for Taiwanese. In order to estimate a worst-case of phthalate migration, food was covered with polyvinyl chloride (PVC) films and then microwave heated. Results show that DEHP level in food increased significantly after heating for 3 min. Under the heating condition, the calculated intake of phthalate and the percentage of the tolerable daily intake (TDI, based on body weight of 60 kg) from eating one 400-g meal were 1705.6 microg and 92.2% for DEHP. Determination of urinary metabolites from 60 subjects reveals more than 90% of samples were detectable for mono-methyl phthalate (MMP), mono-butyl phthalate (MBP) and mono-ethylhexyl phthalate (MEHP). Notably, the median value of estimated daily intake of DEHP had reached 91.6% of TDI established by the European Union Scientific Committee for Toxicity, Ecotoxicity and the Environment (CSTEE) (1998). Thirty-seven percent of the study population exceeded the TDI and 85% exceeded the reference dose (RfD) of the US EPA. We conclude that the body burden of DEHP for Taiwanese reflects the intensives use of plastic materials in the region. The regulation of PVC for food preparation is necessary.     

Hair mercury levels in an urban population from southern Italy: fish consumption as a determinant of exposure

Mercury levels in hair of a general population, 237 adults aged between 35-45, in Naples, Italy, were assessed. The subjects were asked to fill in a questionnaire about age, gender, body weight, height, body mass index (BMI), fish consumption, number, surface and area of dental amalgam fillings. Total mercury (THg) concentrations in human hair ranged from 0.221 to 3.402 microg/g and the mean value for the subjects under study was 0.638 microg/g. Study participants were divided into three groups in accordance with fish consumption and dental amalgam: ANF (amalgam and no fish); NAF (no amalgam but with fish) and AAF (amalgam and fish). Significant differences in THg were found in the three groups (p<0.05). A multiple linear regression analysis showed a weak but significant correlation of THg content in hair with respect to gender and age, but almost no association was found between THg and dental fillings. Conversely, a strong correlation was obtained between THg and fish consumption regardless of the group evaluated. Finally, mercury levels in hair exceeded the levels corresponding to the EPA reference dose (RfD) of 0.1 microg Hg/kg body weight per day (1 microg Hg/g hair) in 6% of the population (4% men and 2% women). However, the THg limits in our subjects were not exceeded according to the WHO guidelines, which use a benchmark dose of 0.23 microg Hg/kg bw/day (14 microg Hg/g maternal hair).     

Specific Features of the Dynamics of Murine Rodent Communities under the Effects of Urbanization: 2. Maintenance of Population Size

The reproduction dynamics of nonsynanthropic rodents living in urbanized areas was compared with that of rodents from natural communities. The number of females in a community and the number of embryos and placental spots per female were estimated. The mechanisms providing for the maintenance of population size were different in different habitats. In particular, the urban communities of murine rodents proved to have a higher reproductive potential.     

The Toad-Headed Agama Phrynocephalus guttatus (Reptilia: Agamidae): The Correlates of Survival in a Translocated Population in the Southwestern Kalmyk Republic

Observations of a translocated population of toad-headed agamas (Phrynocephalus guttatus) in the south of the Kalmyk Republic (1998–2001) have shown that seasonal differences in animal survival are manifested only in juveniles and the survival rate of juveniles is higher than that of adult individuals. A significant correlation between survival rate and sex is also characteristic only of juveniles: survival rate is higher in females than in males. In adult males, survival positively correlates with the level of activity; in juvenile males, with body size. In adult females, survival inversely correlates with body size and weight.     

A Method for Forecasting the Dates of Mass Calving and Some Characteristics of the Rut and Calving in the Kalmyk Population of Saiga (Saiga tatarica L.)

A method for forecasting the dates of calving in the saiga population on the basis of average fetal body weight is proposed. The method is based on the relationship between the number of days before calving and the average weight of fetuses, which obviates the necessity of determining fetal age and exact gestation period in this species. The average length of gestation, the dates of the rut in the years studied, and the age of females that have mated immediately upon the onset of the rut and before its cessation have been determined. It is shown that the duration of mass calving depends on the proportion of mature males in the population.     

Concentrations of PCDD/PCDFs and PCBs in fish and seafood from the Catalan (Spain) market: estimated human intake

The concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and biphenyls (PCBs) in samples from 14 fish and seafood species widely consumed by the population of Catalonia, Spain, were measured. These samples were randomly purchased independently of their geographical origin. The intake of PCDD/Fs and PCBs through consumption of these species was also estimated for various age and sex groups of this population. The highest and lowest levels of PCDD/Fs and dioxin-like PCBs (DL-PCBs) were found in red mullet and shrimp, respectively. For a standard adult man (70 kg body weight), the intake of PCDD/Fs plus DL-PCBs through consumption of fish and other seafood was estimated to be 38.0 pg WHO-TEQ/day. Tuna, hake, and sardine were the species with the highest contribution to this intake. The results of this study indicate that, in general terms, the dietary habits of the population of Catalonia (Spain) regarding fish and seafood consumption do not contribute remarkably to increase PCDD/F and DL-PCB intake.     

基于正态云模型和熵权的人口发展现代化程度综合评价

为有效地监测和评估人口发展现代化程度,实现人口、经济、环境协调发展,探索和构建了人口发展程度综合评价模型。针对人口发展过程中存在的模糊性和随机性问题,建立了基于正态云模型和熵权的综合评判模型。借鉴已有理论的基础上,综合考虑人口总量、人口结构等5个方面因素构建人口综合发展现代化程度指标体系,采用熵权法确定各个指标权重,借助于正态云模型定量描述单指标条件下待评价我国人口发展的等级。云模型是表示某个定性概念与定量表示之间不确定性转换的模型,它将概念的模糊性和随机性结合在一起,对解决不确定性问题具有很强的鲁棒性。采用云模型理论实现评语与评估指标值之间的不确定映射,保留了评估过程中的随机性和模糊性。最后,以2000-2009年我国10年人口发展相关指标的时间序列数据,以及2009年全国和四个直辖市的人口综合发展截面数据为例,对人口发展现代化程度进行实证检验,结果表明,该模型比传统的模糊综合法能得到更加合理的评估结果。     

Normalized Brain Weight as a Characteristic of the Relationship between the Body and Brain Weights in Birds

The growth of many internal organs of vertebrates is isometrically related to the growth of the body. Therefore, calculation of the index of an organ makes it possible to eliminate the basic dependence of organ weight on body weight and to reveal the effects of second-order factors. The relationship between the brain and body weights is allometric in all vertebrates, from fishes to birds and mammals, including man. To make this relationship isometric, brain weight should be divided by the square root of body weight, rather than body weight proper. This parameter was previously named normalized brain weight (NBW). The advantage of using NBW instead of cerebral index has been demonstrated for 88 subarctic populations of different bird species.     

Bioaccumulation of lead in Xenopus laevis tadpoles from water and sediment

The overall objective of this research was to monitor the uptake kinetics of lead in an amphibian model and correlate metal content with embryo development. Based upon the concentration of lead found in the water and sediment of a Louisiana swamp adjacent to a Superfund site, a controlled laboratory experiment exploring lead uptake from water and sediment by Xenopus laevis tadpoles was conducted. For 5 weeks, tadpoles were exposed to water and a simulated sediment, kaolin, spiked with 1, 5, or 10 times the concentration of lead found in field water and sediment samples. Additionally, organisms were exposed to the 5 x condition for 3 and 6 weeks. The experimental controls consisted of unexposed tadpoles and ones exposed to lead originating from water or sediment exclusively. At the end of the exposure periods, developmental data, i.e., body weight and developmental stage, were recorded, and the tadpoles were analyzed for whole body lead concentration. Lead extraction was accomplished by dry ashing, and its amount was quantified polarographically. Results showed that lead inhibited the normal development of these amphibians, in a manner that generally was more severe as exposure level increased. The hindrance of tadpole development also coincided with an increase in whole body lead concentration at higher exposures. Temporally, at the 5 x exposure concentration, the mean lead level increased with time, but this difference was not statistically significant (P<.05). Additionally, control animals exposed to lead (either in water or in sediment) showed no statistical difference with regard to weight and lead uptake, indicating that lead originating from both water and sediment is incorporated into the tadpole. The controlled laboratory experimental protocol used here is thus capable of investigating the uptake of a single metal (Pb in this case) and determining its effect on the development of tadpoles while differentiating the significance of multiple sources of exposure.     

Market basket study on dietary intake of PCDD/Fs, PCBs, and PBDEs in Finland

We have measured the concentrations of polychlorinated dibenzo-p-dioxins (PCDD/F), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDE) in 10 market baskets consisting of almost 4000 individual food samples representing 228 different food items, and also in the total diet basket. Lower bound concentrations of PCDD/Fs ranged between 0.0057 and 5.6 pg/g fresh weight in the market baskets and the corresponding values for PCBs from 39 to 25,000 pg/g. The fish basket contributed most to the concentrations of dioxins and PCBs, and also to concentrations of PBDEs in which the lower bound range was from 0.82 to 850 pg/g. We also assessed the average daily intakes of these substances by the Finnish adult population. The average daily intake of sum of PCDD/Fs and PCBs as WHO toxic equivalents was assessed to be 115 pg, which was 1.5 pg WHO-TEq/kg body weight using an average mean weight of 76 kg for the general population in Finland. The contribution of fish to the intake of PCDD/Fs was between 94% and 72%, depending on whether lower or upper bound concentrations were used. With respect to PCBs, the contribution of fish was 80%. The calculated intake of PBDEs of 44 ng/day was comparable to intake assessments from other countries. Fish also contributed most to the PBDE intake, but there was some other source of PBDEs that distinguishes the exposure to PBDEs from exposure to PCDD/Fs and PCBs. This additional source seemed to be found in the market basket that included beverages, spices, and sweets.     

长江靖江段沿岸似鳊生长及种群参数估算

2012年1月~2013年12月连续24个月在长江靖江段沿岸收集似鳊标本2 334尾,采用基于体长频率数据的ELEFAN I技术估算长江近口段沿岸似鳊的生长参数与种群参数。结果表明:似鳊体长(L,cm)与体质量(W,g)幂函数关系为:W=0.0182×L~(2.948)(r=0.9823,n=2334)。Von Bertalanffy生长方程描述的似鳊生长参数为:极限体长(L_∞)=19.25 cm,生长系数(K)=0.2/a,理论生长起点年龄(t_0)为-1.59 a,体质量的生长拐点年龄为3.55 a。采用Pauly的经验公式估算似鳊自然死亡系数(M)为0.556/a。由长度变换渔获曲线法估算出的似鳊总死亡系数(Z)为1.638/a,捕捞死亡系数(F)和开发率(E)分别为1.082/a和0.66/a。2012~2013年长江靖江段沿岸似鳊年均资源重量和资源数量分别为120.59 t和947.4万尾。经相关估算参数和相对单位补充渔获量分析得出,当前长江靖江段沿岸似鳊已处于过度捕捞状态。建议将沿岸水域禁渔期延长至9月(即4~9月),以实现似鳊资源的恢复和可持续发展。     

Elevation-dependent genetic variation of plants and seed embryos in the Crimea Mountain population of Pinus pallasiana D. Don

In the Crimea Mountain population of Pinus pallasiana D. Don, the genetic structure of adult plants and embryos was studied along an elevation gradient (150, 400, 600, and 900 m a.s.l.). Ten polymorphic loci of six enzyme systems were examined. The degree of differentiation of plants from different elevations and their progeny with respect to genetic structure proved to be low, indicating the absence of strict isolation barriers that would interfere with gene flow along the elevation gradient.     

Factors influencing wing size and body weight variation in the western honeybee

The patterns of wing size and body weight variation have been studied in worker bees, queens, and drones of the western honeybee. The influences of physiological states and ecological situations on the weight of the body and its parts and on the filling of the digestive tract have been analyzed. The results show that the differences in body weight between worker bees, queens, and drones do not correspond to differences in wing size between them. It is noted that worker bees, with their relatively small but strongly varying body weight, have acquired relatively large wings in the course of species phylogeny, a process accompanied by functional and morphophysiological differentiation of the members of bee families.     

Perfluorinated compounds in seafood from coastal areas in China

Diet is an important source of perfluorinated compound (PFC) exposure and seafood is an important diet component for coastal populations. Therefore, it is necessary to monitor the concentrations of PFCs in seafood. In this study, we measured thirteen PFCs in 47 fatty fish and 45 shellfish samples collected from six coastal provinces in China (Liaoning, Shandong, Jiangsu, Zhejiang, Fujian, and Guangdong), using an ultra-performance liquid chromatography and tandem mass spectrometry (UPLC–MS/MS). Perfluorooctanesulfonate (PFOS) was the dominant PFC in fatty fish which accounted for 38% of total PFCs, whereas perfluoroctanoic acid (PFOA) was the predominant PFC in shellfish. Concentrations of PFOS were ranged from less than 1.4 to 1627 pg/g wet weight in fatty fish, with the highest concentration in red drum from Jiangsu. Concentrations of PFOA in shellfish ranged from less than 5.4 to 7543 pg/g wet weight, with the maximum concentration found in briny clam also from Jiangsu. Compared with other studies, the PFC levels in seafood collected from our studied areas are relatively low. The estimated dietary intakes (EDI, pg/kg body weight /day) of PFOA, PFOS and total PFCs for the reference man (63 kg body weight) were calculated by multiplying the mean concentrations (pg/g wet weight) of PFOA, PFOS and total PFCs in six coastal provinces with the daily consumption data (g/day) from the fourth Chinese total diet study carried out in 2007. The highest EDI of PFOS and PFOA were found to be 694 pg/kg body weight/day and 914 pg/kg body weight/day in Guangdong and Jiangsu, respectively. However, the highest EDI of total PFCs was found in Fujian at 2513 pg/kg body weight/day. The EDI from seafood is much lower than the tolerable daily intake (TDI) recommended by the European Food Safety Authority in 2008 indicating low health risk of PFC exposure via eating seafood among the coastal populations in China.     

Variation in Morphophysiological Traits of Male and Female <Emphasis Type="Italic">Aporia crataegi</Emphasis> L. (Lepidoptera: Pieridae) Depending on the Timing of Adult Emergence

The correlation between the timing of adult emergence and variation in morphophysiological parameters of males and females has been analyzed in the black-veined white Aporia crataegi L. In 2013 to 2015, black-veined white butterflies were collected from a natural population in the south of Sverdlovsk oblast throughout the emergence period; at the same time, adult insects were reared from pupae in individual cages. Comparison of the field observations in nature and the results of rearing preimaginal stages has shown that the largest adults are the first to emerge, while the smallest are the last to emerge. This pattern holds true both for the parameters varying throughout the adult insect life (body weight and relative abdomen weight) and for the permanent characteristic of the “structural” size (wing area). The influence of protandry and food availability to caterpillars on the variation in the size of adult A. crataegi during the period of generation emergence is discussed.     

Changes in the abundance of parasitic gamasid mite Macronyssus corethroproctus (Oudemans, 1902) during the overwintering period of its host, the pond bat Myotis dasycneme (Boie, 1825)

The population dynamics of the parasitic gamasid mite Macronyssus corethroproctus (Oudemans, 1902) during the overwintering period of its host, the pond bat Myotis dasycneme (Boie, 1825), has been analyzed. Regression equations have been obtained that describe the dependence of the abundance of mites on the time since the start of overwintering and on host??s sex and body condition.     

Deleterious effects in mice of fish-associated methylmercury contained in a diet mimicking the Western populations' average fish consumption

Methylmercury (MeHg) is a potent neurotoxin, and human beings are mainly exposed to this pollutant through fish consumption. Only a few contradictory epidemiological studies are currently available examining the impact of fish consumption on human populations. In the present study, we wanted to address whether a diet mimicking the fish consumption of Western populations could result in observable adverse effects in mice, and whether beneficial nutriments from fish were able to counterbalance the deleterious effects of MeHg, if any. In Europe and the United States, fish consumption varies widely between countries, from 11 to 100 g fish/day. A mid-range value of 25 g fish/day corresponds to a fish contribution to the total diet of 1.25% on a dry weight basis. We decided to supplement a vegetarian-based mouse diet with 1.25% of lyophilized salmon flesh (SAL diet), or 1.25% of a blend of lyophilized cod, tuna, and swordfish (CTS diet). Total mercury contents were 1.15±0.15, 2.3±0.1 and 35.75±0.15 ng Hg/g of food pellets for the control, SAL and CTS diets, respectively. After two months feeding, the CTS diet resulted in significant observable effects as compared to the control and SAL diets, encompassing decreased body growth, altered behavioral performance and increased anxiety level, modification of mitochondrial respiratory protein subunit concentrations in kidney and brain structures, modified gene expression patterns in kidneys, liver and muscles, and a decrease of dopamine concentrations in the hypothalamus and striatum. Our findings have health implications, firstly because 1.25% of CTS flesh in the diet corresponds to an average exposure to MeHg below the WHO provisory tolerable weekly intake (PTWI) (1.6 μg MeHg/kg of body weight/week), and secondly because many people in Western populations, among them women of child-bearing age, are exceeding the PTWI value (for instance, 35% of the French population inhabiting the Atlantic and Mediterranean coasts).     

Ecological Groups of the Daubed Shanny <Emphasis Type="Italic">Leptoclinus maculatus</Emphasis> (Fries, 1838), an Arcto-boreal Species,Regarding Growth and Early Development

The patterns of growth and development have been studied in L. maculatus early life stages of different ecological groups (pelagic and demersal) from Kongsfjorden and Billefjorden (the northwestern coast of West Spitsbergen). It has been found that the growth rate of larvae markedly increases by age 2+ (stage L2), which is due to their transition to exogenous feeding. Consideration is given to changes in the length and condition of the lipid sac (a provisory organ) in the period of its formation, growth, and resorption in the course of ontogeny. The lipid sac reaches the maximum size at stage L4* and is resorbed at stage L5. Analysis of individual variation in the size and body weight of fish early life stages provides the possibility to evaluate the adaptation capacity of a species under specific conditions of the Arctic.     

Rates of energy and phosphorus metabolism in Littorina snails (gastropoda)

Energy metabolism and phosphorus excretion were studied in Littorina littorea and L. saxatilis snails from the White Sea. Some elemental components of the body were determined quantitatively, and the rates of oxygen consumption and phosphorus excretion were estimated. All parameters were calculated relative to dry body weight (mg). Daily expenditures for energy metabolism in Littorina spp. averaged 3.6 ± 1.1%, and those for phosphorus metabolism were approximately four times lower (about 1%), with the total phosphorus content averaging 0.8% dry body weight. The atomic ratio of consumed oxygen to excreted phosphorus for both species averaged O: P = 1164 ± 140.