环毛蚓在高羊茅修复土壤菲污染过程中的作用

为了解土壤动物在植物修复多环芳烃类化合物过程中的作用,采用盆栽试验法,对比研究了蚯蚓（Pheretima sp．）活动对高羊茅（Festuca arundinacea）修复土壤菲污染的影响效应以及各种生物、非生物因子在植物修复菲污染过程中的作用。结果显示,在20．05-322．06mg·kg^-1菲污染水平范围内,与相同污染水平下无蚯蚓作用的菲污染土壤中生长的植株相比,蚯蚓活动促进修复植物高羊茅的生长：试验期间（72d）,修复植物的单株生物量增加9．74％～21．53％,根冠比增加17．26％～21．44％。添加蚯蚓72d后,种植高羊茅的菲污染土壤中,菲的去除率高达61．70％～88．78％,其平均去除率（77．38％）比无蚯蚓活动的土壤-植物系统中的（68．36％）提高了9．02％,比无植物生长的对照组土壤（22．57％）提高54．81％。各种生物（如植物代谢、植物积累、动物积累、微生物降解、植物．微生物交互作用等）、非生物（如渗滤、吸附、光解、挥发等）因子中,植物．微生物交互作用对菲去除的平均贡献率（47．81％）最为突出,比无蚯蚓活动时（42．08％）提高5．73％。说明蚯蚓活动可强化土壤．植物系统对土壤菲污染的修复作用。     

菲、芘、1,2,4-三氯苯对蚯蚓的急性毒性效应

测定了草甸棕壤条件下 ,菲、芘和 1,2 ,4-三氯苯单一与复合污染对蚯蚓的急性致死及亚致死效应。结果表明 ,3种化学品的浓度与蚯蚓死亡率显著相关 (α =0 .0 5 ,r菲 =0 .87,r芘 =0 .85 ,r三氯苯 =0 .95 ) ,与蚯蚓亚致死效应的相关性稍低 (α =0 .0 5 ,r菲 =0 .75 ,r芘 =0 .72 ,r三氯苯 =0 .85 )。蚯蚓个体对 3种有机物毒性的耐受程度差别较大。引起蚯蚓死亡的毒性阈值浓度菲和 1,2 ,4-三氯苯均为 2 0mg·kg-1,芘为 2 0 0 0mg·kg-1;引起蚯蚓体重增长率下降的亚急性毒性阈值浓度分别为菲 2 0mg·kg-1、1,2 ,4-三氯苯 3 0 0mg·kg-1、芘 10 0 0mg·kg-1;LC10 0 分别为菲 10 0mg·kg-1,1,2 ,4-三氯苯 3 5 0mg·kg-1。由于芘的低水溶性和低毒性 ,无法计算LC10 0 。实验还表明 ,复合污染主要表现为协同和拮抗作用     

生物修复PAHs污染土壤对酶活性的影响

在PAHs污染土壤生物修复过程中,以黑麦草、苜蓿为修复植物,固定化微生物菌剂为外源微生物,通过盆栽实验研究了不同处理对土壤酶活性的影响,以及酶活性与PAHs的去除效果之间的相关性。结果表明,植物修复、微生物修复及两者联合修复均显著提高了土壤PAHs的去除效果,其中黑麦草和苜蓿与微生物菌剂联合修复效果分别达到37.57%和38.41%,比单独的植物修复和菌剂修复高出一倍左右。各种生物修复同时促进了土壤多酚氧化酶、脱氢酶及脲酶的活性,减少了过氧化氢酶活性。过氧化氢酶活性与多酚氧化酶活性呈显著负相关（P〈0.05）、而脲酶与多酚氧化酶显著正相关（P〈0.05）。进一步分析表明PAHs的去除率与脱氢酶活性极显著正相关（P〈0.01）,与多酚氧化酶活性呈显著正相关（P〈0.05）,与过氧化氢酶活性呈负相关（P=0.564）;因此,在PAHs污染土壤生物修复过程中,可以选择土壤脱氢酶活性和多酚氧化酶活性作为PAHs修复效率的微生态指示指标。     

固定化微生物对土壤中苯并芘的降解

研究了3株细菌与3株真菌对土壤中苯并芘（BaP）的降解动态,从中筛选出1株细菌（Bacillus sp.）和1株真菌（Mucor sp.）,并采用吸附法将混合菌固定在改性后蛭石上,研究了固定化混合菌对土壤中BaP的降解效果。结果表明：细菌中芽孢杆菌（Bacillus sp.,SB02）降解率最高,42 d对B[a]P的降解率为33.0%,降解速率也最快,1周可降解12.6%的BaP;真菌中毛霉（Mucor sp.,SF06）降解率最高,42 d对B[a]P的降解率为69.7%;以改性后蛭石为载体用吸附法制得的固定化混合菌,传质性能好,对BaP的降解率42 d可达95.32%,高于游离菌20个百分点。     

Accumulation and phytoavailability of benzo[a]pyrene in an acid sandy soil

Effects of benzo[a]pyrene (B[a]P) on ryegrass (Lolium perenne L.) growth, plant accumulation and dissipiation of B[a]P in a red sandy soil (Hapli-Udic Argosol) were studied in a pot experiment. The plants were grown for 61 days in soil spiked with B[a]P at 0, 12.5, 25 and 50 mg kg⁻¹. Control pots without plants were also set up. Soil extractable B[a]P, plant shoot and root biomass, and concentrations of B[a]P in plant shoots and roots were determined. Ryegrass biomass was increased by addition of B[a]P and root B[a]P concentrations were significantly correlated with B[a]P application rate, but no such correlation was found for shoot B[a]P concentrations. This indicates that B[a]P enhanced the growth of the ryegrass. The extractable B[a]P concentration in the planted soil was significantly lower than that in the unplanted control soil at the rate of 50 mg B[a]P kg⁻¹. This indicates that ryegrass may help to dissipate B[a]P in soil at concentrations over 50 mg kg⁻¹ soil although the mechanism for this is not understood.     

污染场地土壤多环芳烃（PAHs）生物可利用浓度的健康风险评价方法

随土壤不慎经口摄入是PAHs对人体健康造成危害的重要途径之一,目前该暴露途径下PAHs的健康风险计算主要基于土壤中总PAHs浓度进行计算。但是,这种计算方法并未考虑PAHs在土壤中的赋存状态及经口摄入后在人体不同器官中的毒理动力学过程,导致计算结果过于保守,修复目标过于严格,修复成本过高。针对这一问题国外相关研究人员已开展基于土壤中PAHs生物可利用性的健康风险评价研究并取得较大进展,但在国内就如何在风险评价过程中引入PAHs生物可利用性及其面临的障碍缺乏系统性报道。在对土壤中PAHs赋存形态及其随土壤经口腔摄入后在人体消化及循环系统中的动态分配最新研究成果进行综述的基础上,通过对该暴露途径下现有风险计算模型存在问题及原因进行分析,提出基于土壤中PAHs可利用浓度进行风险计算,并对相应计算模型进行推导以及模型参数的获取方法进行了简要概括,以解决目前模型计算结果过于保守的问题。同时,对于在现有分层次进行场地健康风险评价思路中如何科学地纳入基于土壤中PAHs可利用浓度进行风险计算的思路以及在实际风险评价中应用该思路还需进行的研究工作进行了简要讨论。     

Assessment of Cs and Sr accumulation in two epiphytic species of Tillandsia (Bromeliaceae) in vitro

Multiple nuclides commonly occur together and exert toxicity simultaneously, but the difference between single and combined effects of the nuclides is rarely investigated. Epiphytic Tillandsia species are efficient air pollution biomonitors, but rarely used to monitor nuclide contamination. Two Tillandsia species, that is, T. brachycaulos and T. stricta, were chosen to test their capacity to accumulate Cs and Sr. Most plants were able to endure Cs and Sr stress for a long period, which suggested these species could resist toxic elements physiologically and metabolically. With the increasing Cs or Sr concentrations, nuclide contents in both species increased significantly, indicating the potential of Tillandsia species in monitoring nuclide pollutants. However, when the plants were treated with combined nuclides, the content of each ion decreased distinctly compared to those treated with single ion, which suggested Cs and Sr influenced and inhibited each other. In addition, T. brachycaulos seemed more efficient in the uptake of Sr, while T. stricta was more efficient for Cs. Both species accumulated more Sr than Cs at low concentrations, while more Cs than Sr at high concentrations. These results indicated that the uptake of Cs and Sr was related to both the concentrations of the nuclides and the plant species exposed.     

污染场地土壤多环芳烃(PAHs)生物可利用浓度的健康风险评价方法

随土壤不慎经口摄入是PAHs对人体健康造成危害的重要途径之一,目前该暴露途径下PAHs的健康风险计算主要基于土壤中总PAHs浓度进行计算。但是,这种计算方法并未考虑PAHs在土壤中的赋存状态及经口摄入后在人体不同器官中的毒理动力学过程,导致计算结果过于保守,修复目标过于严格,修复成本过高。针对这一问题国外相关研究人员已开展基于土壤中PAHs生物可利用性的健康风险评价研究并取得较大进展,但在国内就如何在风险评价过程中引入PAHs生物可利用性及其面临的障碍缺乏系统性报道。在对土壤中PAHs赋存形态及其随土壤经口腔摄入后在人体消化及循环系统中的动态分配最新研究成果进行综述的基础上,通过对该暴露途径下现有风险计算模型存在问题及原因进行分析,提出基于土壤中PAHs可利用浓度进行风险计算,并对相应计算模型进行推导以及模型参数的获取方法进行了简要概括,以解决目前模型计算结果过于保守的问题。同时,对于在现有分层次进行场地健康风险评价思路中如何科学地纳入基于土壤中PAHs可利用浓度进行风险计算的思路以及在实际风险评价中应用该思路还需进行的研究工作进行了简要讨论。     

Degradation of benzo[a]pyrene in an experimentally contaminated paddy soil by vetiver grass (Vetiveria zizanioides)

A pot experiment was conducted to study the effect of growing vetiver grass on the biodegradation of benzo[a]pyrene (B[a]P) under glasshouse conditions. Plant biomass, microbial biomass C and degradation of B[a]P were determined. B[a]P disappeared faster in the plant treatments than in unplanted controls. Disappearance of B[a]P was accompanied by an increase in soil microbial biomass C. Vetiver grass may promote the biodegradation of B[a]P under flooded conditions by plant roots by stimulating the microbial biomass. Microbial biomass was the main factor affecting dissipation of B[a]P under flooded conditions.     

Concentrations of arsenic and heavy metals in vegetation at two abandoned mine tailings in South Korea

Untreated abandoned mines may result in hazards to ecosystems due to dispersion of various toxic elements such as arsenic (As) and lead (Pb). Phytoremediation is an alternative of remediation for large scale mine dumps. Plant species were sampled from two abandoned gold (Au) mines in South Korea. Plant samples were digested following the guidelines of US EPA Method 3050 (US-EPA, 1996) and analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Concentrations and bioaccumulation factors of plants are reported and out-performing species are summarized. Poplar trees (Populus davidiana) growing on the Myoungbong tailings were suggested to be a potential species for revegetation of large scale Au mine tailings. Arsenic accumulations of bracken ferns (Pteridium aquilinum) sampled from the Duckum tailings were far lower than those of the reported hyperaccumulators, but the possible chronic adverse effects on residents through daily diet are of concern.     

Phytotoxicity and persistence of metribuzin residues in black soil

A bioassay procedure based on the root and shoot growth of cucumber and sorghum were used to study the phytotoxicity, as affected by varying the concentration of metribuzin, and persistence of metribuzin residues in soil. Sorghum and cucumber were found to be very susceptible test plant species to metribuzin. It was found that the persistence and phytotoxicity increased with increasing rate of application of metribuzin, and the relationship between herbicide concentration and phytotoxicity was best described by a quadratic equation. Sorghum and cucumber plants emerged as very sensitive new bioassay plants for metribuzin and could detect residues even at 0.010 and 0.046?ppm in the post harvest soil of potato crop.     

Ecotoxicological evaluation of sediments by battery bioassays: application and comparison of two integrated classification systems

There is a recognised need to integrate multiple toxicity data deriving from battery bioassays into an easily understandable and single value, which may facilitate the decision-making process related to the management of contaminated sediments. In this study, two integration results systems were applied and compared: toxicity classification system (TCS) and Toxicity Test Battery integrated Index (TBI). The quality of sediments of the Taranto seas (Mar Grande and Mar Piccolo) was assessed by a battery of five test species representing different trophic levels (Dunaliella tertiolecta, Tigriopus fulvus, Corophium insidiosum, Mytilus galloprovincialis, and Anphibalanus amphitrite) and performed on three exposure phases (elutriate, porewater, and whole sediment). The integration of biological tests results with TBI showed that stations located in Mar Grande and one station in Mar Piccolo were in the high ecotoxicological risk level, according to the results obtained with TCS. The comparison of results deriving from two evaluation systems showed similarity in the ecotoxicological risk level. Only in one case there was a lack of concordance between two indices giving opposite judgments. The present study demonstrates that the use of one of the two indices can be useful to estimate and to rank hazard level for sediments.     

天津污灌区苯并（a）芘、荧蒽和菲生态毒性的风险表征

运用概率风险评价方法表征和比较了天津污灌区3种多环芳烃对生物影响的风险性。根据各物质的环境暴露浓度和相应急性毒性值的累计概率分布曲线估计了各物质相对风险性的大小。采用联合概率曲线方式比较了不同暴露概率水平条件下3种多环芳烃的相对风险。结果表明，该地区目前菲的总体风险性高于另两种化合物，苯并（a)芘的总体风险性最低。而低暴露风险条件下（受威胁生物不超过20％），苯并（a)芘的风险较大，菲次之，荧蒽风险相对较低。     

Sensitive crop species and appropriate bioassays for potential use in phytotoxicity assessment of Pb-contaminated soils

The present study aims to determine the phytotoxic effects of lead (Pb) on corn (Zea mays), wheat (Triticum aestivum), cucumber (Cucumis sativus), cabbage (Brassica oleracea) and lettuce (Lactuca sativa) and to identify the sensitive crop species and appropriate bioassays for potential use in phytotoxicity assessment of Pb-contaminated soil. In a laboratory experiment, Pb(NO₃)₂ was added to the background soil to obtain eight Pb treatments. The results indicate that the seed germination rate of lettuce decreases by 14.44%, 30.00% and 40.00% at 2000, 3000 and 4000 mg Pb kg^?1 soil, respectively. However, the germination of corn, wheat, cucumber and cabbage is not significantly influenced by the Pb-contaminated soil treated with all the tested concentrations. Furthermore, the root elongation is more sensitive to Pb than is seed germination. The minimum concentrations of adverse effect of maize, wheat, cucumber, cabbage and lettuce are 2000, 3000, 1300, 800 and 300 mg Pb kg^?1 soil, respectively. Moreover, dicotyledon species are more sensitive than monocotyledon species. In the genotoxicity study, the mitotic index (MI) fluctuates with an increasing Pb concentration. The micronuclei (MN) frequencies of cucumber, cabbage and lettuce exhibit a dose-dependent effect at concentrations ranging from 1300 to 4000 mg Pb kg^?1 soil. It can be concluded that lettuce is a good candidate for indicating the toxicity of Pb in soil. Root elongation and the micronucleus frequency of dicotyledon are appropriate bioassays for potential use in phytotoxicity assessment of Pb-contaminated soil.     

东莞市夏季大气颗粒物中的多环芳烃及硝基多环芳烃

以东莞市2011年夏季不同区域的大气颗粒物为研究对象,定性定量分析了其中多环芳烃(PAHs)及硝基多环芳烃(NPAHs)的浓度、组成.采用特征比值法分析了PAHs及NPAHs的来源,并通过PEFs毒性评价法评价了颗粒物中多环芳烃及硝基多环芳烃的BaP等效毒性,估算出个体致癌指数.结果表明东莞市颗粒物上16种多环芳烃总含量在12.60—193.95 ng·m-3范围内,6种硝基多环芳烃的总含量在5.88—62.79 ng·m-3,隧道环境中多环芳烃及硝基多环芳烃的浓度最高.除隧道环境中颗粒物的等效毒性及个体致癌指数超标外,东莞市颗粒物上PAHs及NPAHs对人体均不构成严重威胁.     

改良剂对红蛋植物修复污染土壤重金属铅和镉效果的影响

通过盆栽试验研究了在重度重金属混合污染土壤上,施用不同配比的石灰和泥炭（T1,泥炭0 g·kg^-1土,石灰2 g·kg^-1土;T2,泥炭30 g·kg^-1土,石灰0 g·kg^-1土;T3,泥炭50 g·kg^-1土,石灰0g·kg^-1土;T4,泥炭30 g·kg^-1土,石灰2g·kg^-1土;T5,泥炭50 g·kg^-1土,石灰2g·kg^-1土）对红蛋（Echinodorus osiris）生长及其去除污染土壤铅、镉量的影响,探讨了化学修复和植物提取修复技术相结合修复重金属污染土壤的可能性。研究结果表明,施用石灰显著提高了土壤pH,施用泥炭不显著;施用石灰和泥炭后土壤中交换态铅、镉含量较CK显著降低,红蛋地上部和地下部铅、镉含量较CK有不同程度降低,T4、T5处理降低不显著;T3、T4、T5处理显著地提高了红蛋的铅单株迁移量和年迁移量,年迁移量分别为CK的2.1倍、2.6倍和2.8倍;T1、T2、T3、T4、T5处理显著地提高了红蛋的镉单株迁移量和年迁移量,年迁移量分别为CK的1.8倍、2.9倍、2.9倍、2.8倍和2.9倍,其主要原因在于施用土壤改良剂改善了红蛋的生长,显著增加了地上部生物量。结合经济效益方面因素考虑,以施用泥炭30g·kg^-1土和石灰2g·kg^-1土的处理更适合推广运用。     

城市高架桥降雨径流多环芳烃（PAHs）污染特征及其生态风险评价

以上海市高架桥降雨径流作为研究对象,分析了4场高架路面降雨径流溶解相、颗粒相16种PAHs的质量浓度和场次降雨径流平均浓度（Event Mean Concentration）。探讨了城市高架降雨径流中PAHs质量浓度特征、动态变化过程和污染状况。通过对无量纲累积径流量和无量纲累积污染物负荷曲线M（V）进行幂函数拟合,对4场降雨径流不同环数和总PAHs冲刷强度进行了定量表征。以水样中8种PAHs的监测质量浓度及其对3至40种水生生物的LC50为基础数据,采用推广风险系数法对径流水体PAHs进行了生态风险评价。结果表明：地表径流中溶解态PAHs的质量浓度为27.9~979.4 ng·L-1,主要以3环和4环组分为主;颗粒态PAHs中的质量浓度为1120.1~4892.6 ng·L-1,主要以4-6环的组分为主。径流样品中PAHs更容易吸附在颗粒物上,PAHs主要以颗粒相为主,且4-6环颗粒相PAHs所占比例更高,也显示了化石燃料不完全燃烧的特征。4场降雨径流以2013-05-26次降雨EMC值最大,PAHs污染水平最高。其中通过对BaP的EMC值计算,发现4场降雨径流中BaP的EMC值皆超过了国家的排放标准,应引起相关监测管理部门重视。4场降雨径流均表现不同程度的初始冲刷效应,雨强和径流量是影响初始冲刷的2个重要因素。初始冲刷散点拟合状况良好,定量表征发现2013-06-27次降雨初始冲刷强度最大。风险商表征说明,径流水体PAHs造成的生态风险较小,但部分单体PAH对水生生物的生态影响不容忽视。     

大型钢铁厂及其周边土壤多环芳烃污染现状调查、评价与源解析

采用气相色谱-质谱联机方法(GC-MS)分析了东北某钢铁厂及周边居住区、风景区共11个采样点表层土壤样品16种多环芳烃(PAHs),结果表明,钢铁工业区16种PAHs(∑PAHs)浓度范围为3.39×103—1.54×105ng·g-1,平均浓度3.21×104ng·g-1;居住区∑PAHs浓度范围为587—6.70×103ng·g-1,平均浓度3.82×103ng·g-1;风景区千山∑PAHs浓度385 ng·g-1.∑PAHs和Bap浓度均呈工业区>居住区>风景区趋势.与国内外其他研究结果相比,该钢铁工业区及其周边居住区土壤PAHs污染相对较为严重,11个采样点中有9个采样点土壤∑PAHs为严重污染,4个采样点苯并(a)芘(Bap)浓度超过加拿大土壤质量基准.利用特征比值法(Diagnostic Rate)和主成分分析法(Principal component analysis,PCA)对钢铁工业区及其周边地区土壤进行了源解析,结果表明,钢铁工业区土壤中PAHs主要来源于焦炉、燃煤、柴油燃烧等污染源,周边地区土壤除受工业污染源排放影响外,机动车汽油、柴油污染排放也有重要影响.     

测定5种高环多环芳烃毒性当量因子并应用于太湖梅梁湾表层沉积物分析

作用位点和作用机理相同的污染物通常具有毒性加合作用,但是各个污染物对总体毒性的贡献并不相同,因此需要采用等毒性当量方法进行归一化．本研究选取了5种具有较强芳烃受体效应的多环芳烃(PAHs),通过大鼠肝癌细胞株H4ⅡE体外EROD酶诱导试验,得到相应的剂量／效应关系曲线,并计算了单个PAH的毒性当量因子 (EROD-TEF)．研究结果表明,不同PAH对EROD响应的EC50有较大区别,毒性相对强弱按照苯并[k]荧蒽>茚并 [1,2,3-cd]芘>苯并[a]芘>苯并[b]荧蒽>苊的顺序递减,对应的EROD-TEF值分别为1．1×10-4、3．0×10-5、3．9×10-6、 2．8×10-6和1．5×10-6．利用所得到的EROD-TEF值和气相色谱-质谱(GC-MS)联用方法测定的太湖梅梁湾地区表层沉积物中16种PAHs的浓度,计算得到5种PAHs的2,3,7,8-TCDD毒性当量TEQPAH,并与体外EROD酶诱导生物测试方法测得的表层沉积物毒性当量EROD-TEQ进行了比较．结果显示,两者间存在较好的线性关系(r2=0．65, p<0．05),证明实验测得的EROD-TEF值能够用于实际样品的分析;所研究的沉积物样品中5种PAHs对总芳烃受体效应的贡献均大于50％,表明PAHs是太湖梅梁湾地区表层沉积物中主要的芳烃受体效应活性物质,其可能引起的环境风险应得到进一步重视．