Cadmium in moose kidney and liver – age and gender dependency, and standardisation for environmental monitoring

In the northern hemisphere moose has been found to be suitable as a monitoring animal for the presence of cadmium in the environment. The metal accumulates mainly in the kidney and the liver, with the rate of accumulation dependent on age and possibly also on gender. Collection of tissue material often results in sample selections with disparate age and gender composition, which makes comparison between different regions and different studies difficult. A previous large scale investigation of metals in kidney and liver from moose in Sweden provided Cd data (n = 3,817 and 3,802, respectively) to further explore the relation between Cd accumulation and age/gender. Based on local averages, the individual deviations were analysed with respect to the factors age and gender resulting in an 'ageing function' for each gender and organ. In addition, estimates of the pure individual variations were obtained; the standard deviations correspond to a factor 1.7-1.9 for the Cd concentration, which indicates that 25-30 samples are needed to give a representative mean value (with RSD approximately 10%). In order to be able to compare results from different studies, all individual results can be transformed to represent a 'standard moose' with respect to age and gender. A comparison along these lines was undertaken between Cd levels in Alaska and Sweden. Finally, a relationship between the Cd levels in kidney and liver was derived, providing at least rough estimates for kidney from liver values (or vice versa).     

Mullet and gudgeon liver histopathology and macroinvertebrate indexes and metrics upstream and downstream from a wastewater treatment plant (Febros River—Portugal)

The increased pollution in ecosystems reinforces the importance of both chemical monitoring and biological monitoring of streams and rivers, as an effective water quality-based approach to assess aquatic ecosystem health. In this study, gudgeon (Gobio gobio) and mullet (Mugil cephalus) liver histopathology (biomarker) and some macroinvertebrate community indexes and metrics (bioindicator) were used to evaluate the effect of the wastewater treatment plant (WWTP) of Febros (Avintes) in Febros River water quality and ecosystem health. Regarding macroinvertebrate communities, the Belgian Biotic Index (BBI) and Iberian Biological Monitoring Working Party (IBMWP) indexes suggested that Febros water was slightly polluted, even though the worst situation was found downstream the WWTP discharge. Concerning community metrics, upstream percent of individuals in five numerically dominant taxa (80%) was slightly more superior than the downstream (78%). The presence of intolerant or sensible individuals, determined by percent of Ephemeroptera, Plecoptera, and Trichoptera individuals and number of Ephemeroptera, Plecoptera, and Trichoptera families metrics, was higher upstream WWTP, reflecting a better water quality. The histopathology shows the presence of hepatic lesions in gudgeon and mullet. The statistical analysis of the lesion gradation showed that only necrosis was significantly higher in gudgeon captured downstream the WWTP, while differences were not observed for mullet. The multivariate analysis of data confirmed the existence of differences in hepatic lesions between gudgeon and mullet and between sampling sites. Regarding macroinvertebrate community, this analysis showed that the organic contamination reflected by the BBI and IBMWP indexes values was a determinant factor in the spatial distribution of macroinvertebrates. This work showed that the study of different biological organization levels can be used for a better assessment of ecosystem ecological integrity and can be used as a tool to reveal anthropogenic activity effects in macroinvertebrate diversity and in fish liver pathology from Febros River.     

Influence of home characteristics on airborne and dustborne endotoxin and β-D-glucan

The aim of this study was to assess the associations between airborne and dustborne microbial contaminants (endotoxin and β-D-glucan) and estimate the effects of home characteristics on exposure levels of these microbial contaminants. Endotoxin and β-D-glucan concentrations in airborne inhalable particles, airborne PM1 (<1 μm) and vacuumed dust from 184 residential homes were determined using specific Limulus amebocyte assays. Home characteristics were recorded by visual inspection and questionnaires. Linear regression and correlation analyses were performed. Inhalable endotoxin correlated with dust endotoxin (r = 0.34, p < 0.001) and PM1 endotoxin (r = 0.33, p < 0.001). Inhalable β-D-glucan correlated with dust β-D-glucan (r = 0.18, p < 0.01), but not with PM1 β-D-glucan. Significant correlation was also found between PM1 and dust concentrations for endotoxin (r = 0.26, p < 0.001), but not for β-D-glucan. Multivariate regression analyses showed only one significant association between airborne contaminants and environmental characteristics: inhalable β-D-glucan was positively associated with relative humidity with an effect size (change in the dependent variable corresponding to a unit increase in the independent variable) of 2.32 and p < 0.05. In contrast, several associations were found between dust concentrations and environmental characteristics. Dust endotoxin was positively associated with temperature (2.87, p < 0.01) and number of inhabitants (2.76, p < 0.01), whereas dust β-D-glucan was inversely associated with the presence of dogs (-2.24, p < 0.05) and carpet (-3.05, p < 0.01) in the home. In conclusion, dustborne contaminants were more strongly affected by home characteristics than airborne contaminants. Furthermore, even though statistically significant, the correlations between airborne and dustborne contaminants were weak. This indicates that airborne concentrations cannot be reliably predicted based on dustborne concentrations.     

Source identification and apportionment of PM2.5 and PM2.5−10 in iron and steel scrap smelting factory environment using PMF,PCFA and UNMIX receptor models

To identify the potential sources responsible for the particulate matter emission from secondary iron and steel smelting factory environment, PM_2.5 and PM_2.5?10 particles were collected using the low-volume air samplers twice a week for a year. The samples were analyzed for the elemental and black carbon content using x-ray fluorescence spectrometer and optical transmissometer, respectively. The average mass concentrations were 216.26, 151.68, and 138. 62 μg/m³ for PM_2.5 and 331.36, 190.01, and 184.60 μg/m³ for PM_2.5?10 for the production, outside M1 and outside M2 sites, respectively. The same size resolved data set were used as input for the positive matrix factorization (PMF), principal component factor analysis (PCFA), and Unmix (UNMIX) receptor modeling in order to identify the possible sources of particulate matter and their contribution. The PMF resolved four sources with their respective contributions were metal processing (33 %), e-waste (33 %), diesel emission (22 %) and soil (12 %) for PM_2.5, and coking (50 %), soil (29 %), metal processing (16 %) and diesel combustion (5 %) for PM_2.5?10. PCFA identified soil, metal processing, Pb source, and diesel combustion contributing 45, 41, 9, and 5 %, respectively to PM_2.5 while metal processing, soil, coal combustion and open burning contributed 43, 38, 12, and 7 %, respectively to the PM_2.5?10. Also, UNMIX identified metal processing, soil, and diesel emission with 43, 42 and 15 % contributions, respectively for the fine fraction, and metal processing (71 %), soil (21 %) and unidentified source (1 %) for the coarse fraction. The study concluded that metal processing and e-waste are the major sources contributing to the fine fraction while coking and soil contributed to the coarse fraction within the factory environment. The application of PMF, PCFA and UNMIX receptor models improved the source identification and apportionment of particulate matter drive in the study area.     

Northern Rivers Ecosystem Initiative: Nutrients and Dissolved Oxygen – Issues and Impacts

Anthropogenic inputs of nitrogen (N), phosphorus (P) and oxygen-consuming material to aquatic ecosystems can change nutrient dynamics, deplete oxygen, and change abundance and diversity of aquatic plants and animals. The Northern Rivers Ecosystem Initiative required a research and assessment program to establish the contribution of pulp mill and sewage discharges to eutrophication and depressions in dissolved oxygen (DO) in the Athabasca and Wapiti rivers of northern Alberta, Canada and examine the adequacy of existing guidelines for protecting these systems. Analysis of long-term data showed that total N (TN) and total P (TP) concentrations in exposed river reaches exceeded concentrations in reference reaches by ≤ 2 times for the Athabasca River, and by 9.6 (TP) and 2.6 (TN) times for the Wapiti River. Results from nutrient limitation experiments conducted in situ and in mesocosms showed that benthic algal production was nutrient sufficient downstream of pulp mill discharges but constrained in upper river reaches by insufficient P (Athabasca River) or N + P (Wapiti River). Dissolved oxygen (DO) concentrations in both rivers declined during winter such that median concentrations in the Athabasca River 945 km downstream of the headwaters were approximately 8 mg L⁻¹ in mid-February. Although water column DO rarely approached the guideline of 6.5 mg L⁻¹, DO studies undertaken in the Wapiti River showed that pore water DO often failed to meet this guideline and could not be predicted from water column DO. Results from this integrated program of monitoring and experimentation have improved understanding of the interactions between nutrients, DO and aquatic ecosystem productivity and resulted in recommendations for revisions to nutrient and DO guidelines for these northern rivers.The Canadian Crown reserves the right to retain a non-exclusive, royalty free licence in and to any copyright.     

Polycyclic aromatic hydrocarbon concentrations,mutagenicity, and Microtox® acute toxicity testing of Peruvian crude oil and oil-contaminated water and sediment

The oil industry is a major source of contamination in Peru, and wastewater and sediments containing oil include harmful substances that may have acute and chronic effects. This study determined polycyclic aromatic hydrocarbon (PAH) concentrations by GC/MS, mutagenicity using TA98 and TA100 bacterial strains with and without metabolic activation in the Muta-ChromoPlate? test, and Microtox® 5-min EC₅₀ values of Peruvian crude oil, and water and sediment pore water from the vicinity of San José de Saramuro on the Marañón River and Villa Trompeteros on the Corrientes River in Loreto, Peru. The highest total PAH concentration in both areas was found in water (Saramuro?=?210.15 μg/ml, Trompeteros?=?204.66 μg/ml). Total PAH concentrations in water from San José de Saramuro ranged from 9.90 to 210.15 μg/ml (mean?=?66.48 μg/ml), while sediment pore water concentrations ranged from 2.19 to 70.41 μg/ml (mean?=?24.33 μg/ml). All water samples tested from Saramuro and Trompeteros sites, and one out of four sediment pore water samples from Trompeteros, were found to be mutagenic (P?<?0.001). One sediment pore water sample in Saramuro was determined to have a measurable toxicity (Microtox EC₅₀?=?335.1 mg/l), and in Trompeteros, the EC₅₀ in water and sediment pore water ranged from 25.67 to 133.86 mg/l. Peruvian crude oil was mutagenic using the TA98 strain with metabolic activation, and the EC₅₀ was 17.18 mg/l. The two areas sampled had very high PAH concentrations that were most likely associated with oil activities, but did not lead to acute toxic effects. However, since most of the samples were mutagenic, it is thought that there is a greater potential for chronic effects.     

Pesticide concentrations in water and sediment and associated invertebrate toxicity in Del Puerto and Orestimba Creeks, California, 2007�C2008

The California??s San Joaquin River and its tributaries including Orestimba (ORC) and Del Puerto (DPC) Creeks are listed on the 2006 US EPA Clean Water Act §303(d) list for pesticide impairment. From December 2007 through June 2008, water and sediment samples were collected from both creeks in Stanislaus County to determine concentrations of organophosphorus (OP) and pyrethroid insecticides and to identify toxicity to Ceriodaphnia dubia and Hyalella azteca. OPs were detected in almost half (10 of 21) of the water samples, at concentrations from 0.005 to 0.912 ??g L^???1. Diazinon was the most frequently detected OP, followed by chlorpyrifos and dimethoate. Two water samples were toxic to C. dubia; based on median lethal concentrations (LC₅₀), chlorpyrifos was likely the cause of this toxicity. Pyrethroids were detected more frequently in sediment samples (18 detections) than in water samples (three detections). Pyrethroid concentrations in water samples ranged from 0.005 to 0.021 ??g L^???1. These concentrations were well below reported C. dubia LC₅₀s, and toxicity was not observed in laboratory bioassays. Cyfluthrin, bifenthrin, esfenvalerate, and ??-cyhalothrin were detected in sediment samples at concentrations ranging from 1.0 to 74.4 ng g^???1, dry weight. At DPC, all but one sediment sample caused 100% toxicity to H. azteca. Based on estimated toxicity units (TUs), bifenthrin was likely responsible for this toxicity and ??-cyhalothrin also contributed. At ORC, survival of H. azteca was significantly reduced in four of the 11 sediment samples. However, pyrethroids were detected in only two of these samples. Based on TUs, bifenthrin and ??-cyhalothrin likely contributed to the toxicity.     

Air Pollution Concentrations of PM2.5, PM10 and NO2 at Ambient and Kerbsite and Their Correlation in Metro City – Mumbai

Ambient concentrations of PM_2.5 and PM₁₀ are of concern with respect to effects on human health and environment. Increased levels of mortality and morbidity have been associated with respirable particulate air pollution. In India, it is not yet mandatory to monitor PM_2.5 levels therefore very limited information is available on PM_2.5 levels. To understand the fine particle pollution and also correlate with PM₁₀ which are monitored regularly in compliance with ambient air quality standards. This study was carried out to monitor PM_2.5, PM₁₀, and NO₂ for about one year in a residential cum commercial area of Mumbai city with a view to understand their correlation. The average PM_2.5 concentration at ambient and Kerbsite was 43 and 69 μg/m³. The correlation coefficients between PM_2.5 and PM₁₀ at ambient and Kerbsite were 0.83 and 0.85 respectively thus indicating that most of the PM_2.5 and PM₁₀ are from similar sources. TSP, PM₁₀ levels exceeded Central Pollution Control Board(CPCB) standard during winter season. PM_2.5 levels also exceeded 24 hourly average USEPA standard during winter season indicating unhealthy air quality.     

Adaptation to What and Why?

Adaptation in response to anthropogenic climate change seeks to maintain viability by maximising benefits and minimising losses. It is necessary because some climatic change is now inevitable, despite the international focus on mitigation measures. Indeed, the measures agreed at Kyoto would by themselves result in only a small reduction in the climate changes to be expected over the next century.Discussion of the expected changes and possible impacts leads to the following conclusions regarding climate change scenarios in relation to impacts and adaptation: Climate change in the foreseeable future will not be some new stable "equilibrium" climate, but rather an ongoing "transient" process; Climate change predictions relevant to impacts on most sectors and ecosystems are still highly uncertain; There is a need for a greater focus on developing countries and tropical regions, and on relevant key variables, including the magnitude and frequency of extreme events; The focus should shift from single predictions, or extreme ranges of uncertainty, to risk assessment; Thresholds critical to impacted sectors and ecosystems should be identified, and expressed as functions of climatic variables; Planned adaptations will be necessary to cope with multiple stresses, including those due to non-climatic changes; A major task of adaptation science is to identify the limits of adaptation, i.e., to identify "dangerous levels of greenhouse gases" beyond which adaptation becomes impractical or prohibitively expensive.     

Using remote sensing and GIS to detect and monitor land use and land cover change in Dhaka Metropolitan of Bangladesh during 1960–2005

This paper illustrates the result of land use/cover change in Dhaka Metropolitan of Bangladesh using topographic maps and multi-temporal remotely sensed data from 1960 to 2005. The Maximum likelihood supervised classification technique was used to extract information from satellite data, and post-classification change detection method was employed to detect and monitor land use/cover change. Derived land use/cover maps were further validated by using high resolution images such as SPOT, IRS, IKONOS and field data. The overall accuracy of land cover change maps, generated from Landsat and IRS-1D data, ranged from 85% to 90%. The analysis indicated that the urban expansion of Dhaka Metropolitan resulted in the considerable reduction of wetlands, cultivated land, vegetation and water bodies. The maps showed that between 1960 and 2005 built-up areas increased approximately 15,924 ha, while agricultural land decreased 7,614 ha, vegetation decreased 2,336 ha, wetland/lowland decreased 6,385 ha, and water bodies decreased about 864 ha. The amount of urban land increased from 11% (in 1960) to 344% in 2005. Similarly, the growth of landfill/bare soils category was about 256% in the same period. Much of the city's rapid growth in population has been accommodated in informal settlements with little attempt being made to limit the risk of environmental impairments. The study quantified the patterns of land use/cover change for the last 45 years for Dhaka Metropolitan that forms valuable resources for urban planners and decision makers to devise sustainable land use and environmental planning.     

Accumulation dynamics and cellular locations of Pb, Zn and Cd in resident and transplanted Flavocetraria nivalis lichens near a former Pb–Zn mine

Accumulation dynamics and cellular locations of lead (Pb), zinc (Zn) and cadmium (Cd) were studied in Flavocetraria nivalis lichens near the former Black Angel Pb–Zn Mine in West Greenland. Natural resident thalli were collected from four dust-contaminated sites near the mine. In addition, thalli were taken from an uncontaminated reference site and transplanted to the contaminated sites followed by a collection 1 year after. Total thalli metal contents were determined, and thalli were subjected to a sequential extraction procedure. After 1 year of transplantation, total Pb thalli contents were significantly elevated compared with initial concentrations at all sites (for Zn and Cd contents only at the two sites closest to the mine). However, transplanted thalli contained significantly less Pb (26?±?12 %), Zn (64?±?13 %) and Cd (34?±?7 %) compared with resident thalli from these sites. Results from the sequential extraction procedure showed marked differences among Pb, Zn and Cd in the extracellular, intracellular and residual fraction. The lower total metal concentrations in transplanted compared with resident thalli at the contaminated sites were mostly due to a larger metal content bound in the residual fraction in resident thalli. In contrast, the metal content bound in the extracellular fraction were not significantly different in transplanted and resident thalli. The results indicate that extracellular-bound Pb, Zn and Cd in F. nivalis can be used as a proxy for recent (annual) atmospheric metal deposition whereas the large residual metal fraction in resident lichens indicate an accumulation of metal-containing particles in the thalli over time that includes several years of uptake.     

Comparative toxicity of nano-ZnO and bulk ZnO suspensions to zebrafish and the effects of sedimentation, ˙OH production and particle dissolution in distilled water

With the common application of nanoscale zinc oxide (nZnO) and significant potential for its release directly into aquatic environments, it is urgent to carry out research on ecotoxicological impact of nZnO. The characterization of nZnO, the amount of ˙OH in suspensions in the presence of light and the acute toxicity of nZnO and its bulk counterpart suspensions, as well as the acute toxicity of Zn(2+) solution to zebrafish (Danio rerio) at 96 h were studied. It was found that nZnO aggregated into irregular shapes in suspensions, and showed a relationship between its size distribution and concentration. In the presence of light, nZnO suspensions could generate ˙OH, the concentration of which increased with time. Although it was generally thought that ˙OH played a role in the biotoxicity to zebrafish, similar toxicity was observed for the nZnO and bulk ZnO suspensions (96 h LC(50) 3.969 mg L(-1), 2.525 mg L(-1), respectively). Furthermore, the sedimentation of nZnO and bulk ZnO in suspensions, and the accumulation of Zn in zebrafish were studied. The results showed that dissolved Zn(2+), from nZnO and bulk ZnO in suspensions, were toxic to zebrafish, while the aggregation and sedimentation of nZnO suspensions reduced the toxicity of nZnO. However, Zn(2+) may not be the main source of acute toxicity of nZnO and bulk ZnO to zebrafish. The experimental results highlight the importance of a systematic assessment of toxicity mechanisms of metal oxide nanoparticles (NPs) to determine definitively whether their toxicity is caused by nano-effects.     

Temporal and spatial change detecting (1998–2003) and predicting of land use and land cover in Core corridor of Pearl River Delta (China) by using TM and ETM+ images

Land use/land cover (LULC) has a profound impact on economy, society and environment, especially in rapid developing areas. Rapid and prompt monitoring and predicting of LULC’s change are crucial and significant. Currently, integration of Geographical Information System (GIS) and Remote Sensing (RS) methods is one of the most important methods for detecting LULC’s change, which includes image processing (such as geometrical-rectifying, supervised-classification, etc.), change detection (post-classification), GIS-based spatial analysis, Markov chain and a Cellular Automata (CA) models, etc. The core corridor of Pearl River Delta was selected for studying LULC’s change in this paper by using the above methods for the reason that the area contributed 78.31% (1998)–81.4% (2003) of Gross Domestic Product (GDP) to the whole Pearl River Delta (PRD). The temporal and spatial LULC’s changes from 1998 to 2003 were detected by RS data. At the same time, urban expansion levels in the next 5 and 10 years were predicted temporally and spatially by using Markov chain and a simple Cellular Automata model respectively. Finally, urban expansion and farmland loss were discussed against the background of China’s urban expansion and cropland loss during 1990–2000. The result showed: (1) the rate of urban expansion was up to 8.91% during 1998–2003 from 169,078.32 to 184,146.48 ha; (2) the rate of farmland loss was 5.94% from 312,069.06 to 293,539.95 ha; (3) a lot of farmland converted to urban or development area, and more forest and grass field converted to farmland accordingly; (4) the spatial predicting result of urban expansion showed that urban area was enlarged ulteriorly compared with the previous results, and the directions of expansion is along the existing urban area and transportation lines.     

Heavy metal phytoextraction—natural and EDTA-assisted remediation of contaminated calcareous soils by sorghum and oat

The abilities of sorghum (Sorghum bicolor L.) and oat (Avena sativa L.) to take up heavy metals from soils amended with ethylenediaminetetraacetic acid (EDTA) were assessed under greenhouse conditions. Both plants were grown in two soils contaminated with heavy metals (Gujranwala—silty loam and Pacca—clay loam). The soils were treated with 0, 0.625, 1.25, and 2.5 mM EDTA kg^?1 soil applied at both 45 and 60 days after sowing (DAS); the experiment was terminated at 75 DAS. Addition of EDTA significantly increased concentrations of Cd, Cr, and Pb in roots and shoots, and bio-concentration factors and phytoextraction rates were also increased. Post-harvest soil analysis showed that soluble fractions of metals were also increased significantly. The increase in Cd was ≈ 3-fold and Pb was ≈ 15-fold at the highest addition of EDTA in Gujranwala soil; in the Pacca soil, the increase was less. Similarly, other phytoremediation factors, such as metal translocation, bio-concentration factor, and phytoextraction, efficiency were also maximum when soils were treated with 2.5 mM EDTA kg^?1 soil. The study demonstrated that sorghum was better than oat for phytoremediation.     

Mapping of fluoride endemic area and assessment of F−1 accumulation in soil and vegetation

The prevalence of fluorosis is mainly due to the consumption of more fluoride (F^?1) through drinking water, vegetables, and crops. The objective of the study was mapping of F^?1 endemic area of Newai Tehsil, Tonk district, Rajasthan, India. For the present study, water, soil (0–45 cm), and vegetation samples were collected from 17 villages. Fluoride concentration in water samples ranged from 0.3 to 9.8 mg/l. Out of 17 villages studied, the amounts of F^?1 content of eight villages were found to exceed the permissible limits. Labile F^?1 content and total F^?1 content in soil samples ranges 11.00–70.05 mg/l and 50.3–179.63 μg g^?1, respectively. F^?1 content in tree species was found in this order Azadirachta indica 47.32–55.76 μg g^?1 > Prosopis juliflora 40.16–49.63 μg g^?1 > Acacia tortilis 34.39–43.60 μg g^?1. While in case of leafy vegetables, F^?1 content order was Chenopodium album 54.23–98.42 μg g^?1 > Spinacea oleracea 30.41–64.09 μg g^?1 > Mentha arvensis 35.48–51.97 μg g^?1. The order of F^?1 content in crops was found as 41.04 μg g^?1 Pennisetum glaucum > 13.61 μg g^?1 Brassica juncea > 7.98 μg g^?1 Triticum sativum in Krishi Vigyan Kendra (KVK) farms. Among vegetation, the leafy vegetables have more F^?1 content. From the results, it is suggested that the people of KVK farms should avoid the use of highly F^?1 containing water for irrigation and drinking purpose. It has been recommended to the government authority to take serious steps to supply drinking water with low F^?1 concentration for the fluorosis affected villages. Further, grow more F^?1 hyperaccumulator plants in F^?1 endemic areas to lower the F^?1 content of the soils.     

Combining mapped and statistical data in forest ecological inventory and monitoring – supplementing an existing system

A forest ecological inventory and monitoring system combining information derived from maps and samples is proposed based on ecosystem regions (Bailey, 1994). The system extends the design of the USDA Forest Service Region 6 Inventory and Monitoring System (R6IMS) in the Pacific Northwest of the United States. The key uses of the information are briefly discussed and expected results are illustrated with examples. The system is flexible, allowing regions based on ecological considerations to be modified. Sampling intensities that are affordable are likely to be insufficient to provide meaningful estimates for key parameters relating to rare and endangered species, watersheds, and other ecological units. Methods are proposed for collecting additional information in follow-up surveys and combining it with relevant information obtained in R6IMS. Near-continuous information on weather and possible pollution variables recorded by instruments at sampling sites is needed to develop meaningful models to understand what is happening in the ecoregions. R6IMS and the proposed additions constitute a dynamic system which will be modified further as data are analyzed.     

Contaminant Exposure and Effects—Terrestrial Vertebrates Database: Trends and Data Gaps for Atlantic Coast Estuaries

In order to examine the condition of biota in Atlantic coast estuaries, "Contaminant Exposure and Effects—Terrestrial Vertebrates" database (CEE-TV) has been compiled through computerized search of published literature, review of existing databases, and solicitation of unpublished reports from conservation agencies, private groups, and universities. Summary information has been entered into the database, including species, collection date (1965–present), site coordinates, estuary name, hydrologic unit code, sample matrix, contaminant concentrations, biomarker and bioindicator responses, and reference source, utilizing a 98-field character and numeric format. Currently, the CEE-TV database contains 3699 geo-referenced records representing 190 vertebrate species and >140,000 individuals residing in estuaries from Maine through Florida. This relational database can be directly queried or imported into a Geographic Information System to examine spatial patterns, identify data gaps and areas of concern, generate hypotheses, and focus ecotoxicological field assessments. Information on birds made up the vast majority (83%) of the database, with only a modicum of data on amphibians (<0.1%). Of the >75,000 chemical compounds in commerce, only 118 commonly measured environmental contaminants were quantified in tissues of terrestrial vertebrates. There were no CEE-TV data records in 15 of the 67 estuaries located along the Atlantic coast and Florida Gulf coast. The CEE-TV database has a number of potential applications including focusing biomonitoring efforts to generate critically needed ecotoxicological data in the numerous "gaps" along the coast, reducing uncertainty about contaminant risk, identifying areas for mitigation, restoration or special management, and ranking ecological conditions of estuaries.     

Singapore‘s Environment and Food Production

Most recent figures from Singapore‘s Primary Production Department show that the country now produces less than 10% of its own food. This compares with 40% of its own water supply, hitherto considered to be Singapore‘s Achilles Heel. Although the Republic‘s agrotechnology programme has been in existence since 1986, it appears to be lagging behind demand; so can anything be done to increase production and at the same time improve the environment? This paper examines the recent history of Singapore‘s agricultural policy and compares it with the "traditional" family farms which are still being phased out. It considers that family farms, agricultural allotments and even flat roof tops, could be both economically productive and of great advantage to the environment because of recreational and ecological benefits. The conclusion is that there is a place for both traditional farmland as well as advanced food growing technology in Singapore.