Transfer-RNA: The early adaptor

Evolutionary history of tRNA is studied by comparative sequence analysis of two specified tRNA's at various phylogenetic levels and of tRNA families within four different species. Criteria are developed that allow 1) to distinguish between convergent and divergent evolution, 2) to determine the mechanism of divergence and 3) to estimate the degree of randomization of the variable parts of the sequences. The conclusion of these investigations is that tRNA's represent ancient molecules that existed in the form of a mutant distribution prior to their integration into genomes.     

Qualitative and Quantitative Assessment of Hepatitis A Virus in Wastewaters in Tunisia

Hepatitis A causes substantial morbidity in both industrialized and non-industrialized countries and represents an important health problem in several southern Mediterranean countries. The objectives of the study were as follows: (a) to assess the occurrence of hepatitis A virus (HAV) in Tunisia through the monitoring of urban wastewaters collected at wastewater treatment plants (WTPs); (b) to characterize environmental strains; and (c) to estimate the viral load in raw and treated sewages, in order to evaluate the potential impact on superficial waters receiving discharges. A total of 150 raw and treated wastewaters were collected from three WTPs and analyzed by both qualitative (RT-PCR/nested) and quantitative (qRT-PCR) methods. Of these, 100 (66 %) were found to be positive for HAV by the qualitative assay: 68.3 % in influents and 64.7 % in effluents. The vast majority of HAV sequences belonged to sub-genotype IA, with 11 different strains detected found to be identical to clinical strains isolated from Tunisian patients with acute hepatitis. Five unique variants were also detected, not previously reported in clinical cases. Only two IB strains were found, confirming the rarity of this sub-genotype in this country. The results of the present study indicate a wide circulation of the pathogen in the population, most probably in the form of asymptomatic infections, a finding consistent with the classification of the country as having intermediate/high endemicity. Quantitative data showed high viral loads in influents (3.5E+05 genome copies/liter, mean value) as well as effluents (2.5E+05 genome copies/liter, mean value), suggesting that contaminated water could be a critical element in transmission.     

Cercaricidal activity of some essential oils of plants from Brazil

Extended screening tests on the cercaricidal activity of various plants from northeast Brazil have shown that the extracts of the leaves and fruits ofPiper marginatum,Protium heptaphyllum, andCapsicum annuum show a remarkable effect on the cercaria ofSchistosoma mansoni. In the case of the oils ofPiper marginatum andCapsicum annuum, 90–96% of the cercaria ofSchistosoma mansoni were killed within 15 min. According to the NMR spectra, the active principles of the extracts seem to be water-soluble unsaturated compounds from the oils or their hydrolysis products. Natural substances provide an attractive alternative for preventive actions against schistosomiasis, as the perturbation of the ecological equilibrium of natural waters can be avoided in this manner.     

UVC Inactivation of dsDNA and ssRNA Viruses in Water: UV Fluences and a qPCR-Based Approach to Evaluate Decay on Viral Infectivity

Disinfection by low-pressure monochromatic ultraviolet (UVC) radiation (253.7 nm) became an important technique to sanitize drinking water and also wastewater in tertiary treatments. In order to prevent the transmission of waterborne viral diseases, the analysis of the disinfection kinetics and the quantification of infectious viral pathogens and indicators are highly relevant and need to be addressed. The families Adenoviridae and Polyomaviridae comprise human and animal pathogenic viruses that have been also proposed as indicators of fecal contamination in water and as Microbial Source Tracking tools. While it has been previously suggested that dsDNA viruses may be highly resistant to UVC radiation compared to other viruses or bacteria, no information is available on the stability of polyomavirus toward UV irradiation. Here, the inactivation of dsDNA (HAdV2 and JCPyV) and ssRNA (MS2 bacteriophage) viruses was analyzed at increasing UVC fluences. A minor decay of 2-logs was achieved for both infectious JC polyomaviruses (JCPyV) and human adenoviruses 2 (HAdV2) exposed to a UVC fluence of 1,400 J/m², while a decay of 4-log was observed for MS2 bacteriophages (ssRNA). The present study reveals the high UVC resistance of dsDNA viruses, and the UV fluences needed to efficiently inactivate JCPyV and HAdV2 are predicted. Furthermore, we show that in conjunction with appropriate mathematical models, qPCR data may be used to accurately estimate virus infectivity.     

Transfer-RNA,an early gene?

The theory of self-reproductive molecular systems involves the consequence that translation must have started from a selected distribution of RNA molecules, that comprised GC-rich sequences of a length < 100 nucleotides. This implies a joint function of messenger and adaptor, which both had to be recruited from the same mutant distribution. The reconstruction of tRNA precursors yields such a molecule showing some reverberation of a codon pattern GNC. These findings suggest that tRNA has been the earliest component of the translation machinery.     

Soil infertility in the Amazonian rain forest

Extensive mineral alteration in the older land surfaces of the eastern Amazonian lowland has led to complete impoverishment of the soils in respect of their inorganic nutrient contents. Large-scale use of this area for agricultural purposes seems impossible. In the western Amazonian lowland, younger land surfaces (Pliocene) have developed along with Pleistocene flood plains. The mineral alteration in the soil profiles of this area, which are partly characterized by poor drainage, has resulted in impoverishment of soils to a lesser degree. These soils can be used to a limited extent for agricultural purposes, especially at the level of a subsistence economy.     

Squeezing light out of crystals: Triboluminescence

Triboluminescence is the light emitted when mechanical energy is applied to a crystal. Many common substances such as sugar and copper sulfate are triboluminescent. The origin of the light and the mechanical, chemical, and crystallographic requirements of the phenomenon are described in this paper.     

Comparative psychoacoustics: Perspectives of peripheral sound analysis in mammals

Psychophysical data on hearing in mammals are summarized. The data are then correlated to the anatomy and physiology of the ear. Common mechanisms of sound transfer and analysis in the acoustic system, with stress on the auditory periphery, are discussed.     

Small scale farmers’ vulnerability to climatic changes in southern Benin: the importance of farmers’ perceptions of existing institutions

Farmers in rural Africa use a number of adaptive strategies to cope with observed climatic changes and their impacts on agriculture. Most studies on adaptive capacity focus on socio-economic parameters (such as poverty or education), and few provide detailed analysis on the role played by different institutions at local level, and the effects of how these institutions are perceived on farmers’ adaptation. Semi-structured interviews were conducted among 46 households from seven villages in southern Benin (West Africa), and among representatives of several institutions at the local level. Half the participants were involved in Non Governmental Organizations (NGOs) development projects and half were independent farmers. Results indicate that independent farmers mostly use non-agricultural coping strategies (loans, work in town) while project farmers mainly use agricultural adaptive strategies (improved seed varieties). Lack of adaptive capacity of independent farmers is linked to weak State institutions at the local level. Due to their lack of efficiency and high corruption rates, local State representatives are mistrusted. NGOs are trusted and seek for help, even by independent farmers. Even if NGOs do not have climate change adaptation in their agendas, they promote activities, which help reduce farmers’ vulnerability. Although our results are limited to south-western Benin, they question the way adaptation is promoted today, for instance through the United Nations Framework Convention for Climate Change (through the National Adaptation Programmes of Action).     

Suppressor cells prevent host resistance to tumor growth

The role of immunosuppressor cells in preventing host immune rejection of tumor cells is described. The growth of Lewis lung carcinoma (3LL) in syngeneic C57BL mice is accompanied by a weak and transient anti-tumor cytotoxic response that is later on replaced by tumor-enhancing activity. This enhancing activity is correlated with the generation of suppressor cells in 3LL-bearing mice. Such suppressors were demon strated in two ways: (a) Elimination of the hydrocortisone(HC)-sensitive lymphoid population from tumorbearing mice (TBM) resulted in a significant increase in the anti-tumor cytotoxic response and in a marked delay in tumor development. It is assumed that HC inactivates precursors of suppressor lymphocytes whereas the mature suppressor cells themselves are HC-resistant. (b) The increased resistance against the tumor could be partially re-suppressed by restoring the HC-treated TBM with spleen or thymus cells from normal C57BL. Suppression, however, was more pronounced if the resistant mice were restored with spleen or thymus cells from TBM. HC-resistant spleen cells from TBM that appear to be enriched for mature suppressor cells were capable of suppressing in vitro the secondary sensitization of spleen cells from TBM against tumor cells.     

Early diagenetic alterations of biogenetic organic compounds

Plant matter from a beech forest, leaves, and soil were extracted and analyzed by GC-MS technique. The amount of the extract varies with the CPI value. Characteristic compounds like phytadienes, plant alcohols, polycyclic aromatics, sterols, and triterpenes are observed in different samples at various concentrations. It seems there is a rapid change in the composition of extracts from recent plant material during early diagenesis based on reactions such as hydrolysis, dehydration, oxidation, and polymerization.     

Toward drugs derived from cannabis

Recent work aimed at the introduction of natural and synthetic cannabinoids as drugs is reviewed. Δ¹-Tetrahydrocannabinol (Δ¹-THC) is mainly investigated as a potential drug against glaucoma and asthma, and as an antiemetic agent in cancer chemotherapy. Cannabidiol is being tried in the clinic against epilepsy and as a hypnotic. Numerous synthetic cannabinoids are currently being investigated as analgetics and as sedative-relaxants.     

“Arabidobrassica”: Plant-genome engineering by protoplast fusion

After fusion of somatic cells (isolated protoplasts) of Arabidopsis thaliana and Brassica campestris, plants could be regenerated in which genetic material from parents of taxonomically different tribes is combined. Between these regenerants asymmetric hybrids have also been obtained, in which one parental genome is represented by reduced chromosome numbers. Results concerning the analysis and genesis of these plants are used to discuss the question whether such monster plants are of any importance.     

On the kinetics of acetylcholine at the synapse

Electrophysiologic, morphologic, and biochemical definitions of the synapse will be correlated spatially and temporally. Postsynaptic fatigue and facilitation follow oscillations of the free pool of acetylcholine, predicted by the kinetic theory and observed at the electric organ of Torpedo marmorata. The underlying thermodynamic instability exhibits properties of the Na⁺—K⁺-dependent hydrolysis of adenosine triphosphate and represents a necessary condition for synaptic memory.     

How do neutron stars evolve?

A possible evolutionary history of neutron stars is delineated, ranging from star formation through supernova explosions, X-ray and pulsar stages to an eventual pulsar turnoff via spin alignment, or magnetic field decay. New is the emphasis that there should be two populations of pulsars, the slow and the fast one, according to their successive formation in a binary system. The two pulsar populations are related to two phenotypes of supernova remnants, of which Cas A and the Crab are the best known examples.     

The phylogeny of archaebacteria,including novel anaerobic thermoacidophiles in the light of RNA polymerase structure

DNA-dependent RNA polymerases of archaebacteria are distinct from those of eubacteria both in structure and in function. They show similarities to those of the eukaryotic cytoplasm. Extremely thermophilic anaerobic sulfur-respiring archaebacteria isolated from solfataric waters represent four different families, the Thermoproteaceae, the “stiff filaments”, the Desulfurococcaceae and the Thermococcaceae, of a novel order, Thermoproteales. Together with the Sulfolobales, they form the second branch of the urkingdom of the archaebacteria besides that of the methanogens and extreme halophiles. Thermoplasma appears isolated.     

The lumisphere,a new model of pre-biotic evolution

Lyophilization of the amber-colored, fluorescent oils obtained via thermolysis of amino acid mixtures (Lys, Ala, Gly) leads to a cellular-structured material—with an average cell diameter of 50 μm. The cellular aggregates show a resemblance to epithel tissues. Single cellular entities find their analogs in Fox microspheres as well as in the microfossilsIsuasphaera. Short-wave UV (254 nm) irradiation of the aqueous suspensions destroys the cells, whereas suspension in saline solutions (NaCl) offers protection against dissolution by UV light. The fluorescence spectrum of the microscopic preparations containing the highly fluorescent cellular material (“lumispheres”) was registered.     

A new pheromone of the silkworm moth Bombyx mori

The female silkmoth Bombyx mori L. emits a second pheromone component bombykal (E-10, Z-12-hexade-cadien-1-al) in addition to the well-known sexual attractant bombykol (E-10, Z-12-hexadecadien-1-ol). Bombykal stimulates its own specialized and highly sensitive olfactory cells of the male moth. Surprisingly, the aldehyde inhibits the release of the male's wing-fluttering response to bombykol.     

New results in comet research

Comets are kilometer-sized icy conglomerates of frozen gases and dust particles which move around the Sun in an assembly called “Oort's cloud” in the outer parts of the planetary system. Only those which are ejected towards the Sun by the perturbations of passing stars will evaporate gases and dust while they are near the Sun. This material forms the large coma and the tails which make up the characteristic phenomenon of a comet. The spectral analysis has yielded that water is the main constituent of a comet, other molecules are simple compounds of H, C, O and N. — The ESA is planning a space mission to Comet Halley in 1986.     

Guidelines for the strategic management of flood risks in industrial plant oil in the Brazilian coast: adaptive measures to the impacts by relative sea level rise

This study provides guidelines for strategic management in industrial oil plants linked to uncertainties of climate change through the development of integrated planning methodology with focus on coastal flooding events caused by relative sea level rise (RSLR). The research site is in Redonda Island, located in Guanabara Bay, Rio de Janeiro City, Brazil, and since 1960, it constitutes an industrial oil plant facility. The region suffers interaction with storms and meteorological tides from extratropical cyclones over the South Atlantic Ocean, being vulnerable to risks of disasters, floods, and coastal erosion. A Program on Vulnerability and Adaptation Assessments to Relative Sea Level Rise (“Programa de Avaliação e Adaptação às Vulnerabilidades de Elevação do Nível Relativo do Mar—PAAVENRM”) was developed to avoid compromising the regional and local development in the industrial system of the island, which is an ad hoc instrument designed to anticipate and reduce risks, damages, and losses by occurrence of extreme climatic events in coastal areas prone to flooding caused by RSLR. Results from computer simulation modeling indicate 37 prospective qualitative scenarios that consolidate the conditions of future climate vulnerability of the plant, starting from United Nations Intergovernmental Panel on Climate Change (IPCC) information for RSLR up to 2100. Three quantitative forecasting scenarios were simulated, under boundary conditions preset for different altimetric ranges subject to submersion, based upon ordinary and extraordinary tides measured in the area in relation to RSLR, which allowed the evaluation of the industrial infrastructure at risk. Furthermore, three thematic maps were elaborated for the planning of specific coastal protection interventions. Percentages of physical damage and property losses were estimated. The importance of applying guidelines for medium and long-term corporate strategy management, integrating the risk of flooding, the rigging of civil defense systems, meteorology, and of the plans, programs, and existing systems and others to be developed is highlighted. From this perspective, the proposed scenarios help to identify the most relevant alternatives for mitigation and adaptation under technical criteria for decision making in the study area.