Messung und Quantifizierung von Mineralölkohlenwasserstoffen

Investigations concerning the measurement and evaluation of mineral oil hydrocarbons using Fourier-transform-infrared (FT/IR-)-spectroscopy,¹H-Nuclear magnetic resonance (¹H-NMR)-spectroscopy and Capillary gas chromatography — Flame ionisation detection (GC-FID) are presented. By means of various mineral oils and three certified reference materials (CRM) all tested methods were within a ±7%-range to the mineral oil nominal value and the 95% confidence intervals of the CRM’s, respectively. The GC-FID evaluation could be done without calibration using an relative response ratio of mineral oil to an internal standard (n-tetracontane). A¹H-NMR-method was developed for the quantitative determination of mineral oil hydrocarbons, successfully applied down to 0.2 mg/ml. Due to the determination limit achieved, the¹H-NMR-spectroscopy gain in importance as a reference method for the analysis of mineral oils.     

Abschätzung des umweltchemischen und ökotoxikologischen Verhaltens von Stoffen durch computergestützte Analyse von Struktur und Verhalten sowie von Struktur und Wirkung

Risk assessment of chemicals requires comprehensive data material, which often is not available. To provide substitutes for the lacking experimental ecotoxicological and physico-chemical data, a software-system (SAR-System) has been developed comprising more than 90 estimation models for relevant endpoints. The approach is based on quantitative structure-activity relationships (QSAR). Two major aspects characterize the SAR-System:

1. The implemented models were tested for their validity and application range.
2. The QSARs are accessible by a menu-driven programme package.

The following endpoints are included: Thysico-Chemical Data: 1-Octanol/water-partition-coefficient log P_ow, vapour pressure, water solubility, pK_a-value, boiling point Biological Data: Toxicity towards fish, daphnia, tetrahymenae, algae, bacteria and mammals, mutagenicity. Distribution: soil sorption, bioconcentration, Henry-Constant, Mackay (Level I). Degradation: photodegradation, biodegradability.     

Qualität und Verwertungsmöglichkeiten von Holzaschen aus naturbelassenen Hölzern

Background, aim and scope The increasing use of wood for generating heat and electricity requires that more and more fuels be obtained directly or indirectly from the forest. Sound, sustainable recycling management calls for the return of any generated wood ash back to the forest to make use of the nutrients it contains. Similarly, recycling this ash in other locations such as agricultural land or private gardens can serve equally well as fertilizer. At the same time, it is critical that no accumulated pollutants be introduced into the nutrient loop. Wood ash that is heavily laden with such pollutants must not be considered for recycling. As part of this research project, commissioned by the Ministry for the Environment and Conservation, Agriculture and Consumer Protection of the State of North Rhine-Westphalia in Germany (MUNLV), ash samples of 209 wood-fired appliances generating between 10 and 4000?kW of heat performance were taken from throughout the federal state of North Rhine-Westphalia. Untreated wood, either forested or scrap, was used as the sole fuel for these appliances. Materials and methods All course ash and fly ash samples were analyzed to determine their composition of main nutrients, heavy metals, and the elements Fe, Cl, Si, Al and Na. The purpose of this analysis was to evaluate the suitability of this wood ash for reintroduction to forest soils or as fertilizer in other types of soil. Results The majority of the wood ash samples in this study contained sufficiently high amounts of nutrients to match the requirement for stand-alone fertilizers (PK-fertilizer, potash fertilizer). However, the heavy metal content was highly variable, with a mean content high enough to prohibit them from being classified per se as PK- or potash fertilizer for agricultural land. Due to the high quantities of cadmium, application of this ash to garden soils would likewise be ill-advised. Discussion On forest soils German law permits application of a mixture of potash fertilizer containing, at most, 30?% wood ash (course ash). Because of the high amounts of cadmium and copper, wood ash from our samples can only comprise a maximum of 28?% when added to typically used potash fertilizers. Higher percentage of wood ash would exceed the cut-off value established by the German Fertilizer Ordinance (Düngemittelverordnung – DüMV). Conclusions The application of wood ash on agricultural land and in private gardens is, rightfully so, highly regulated by law. However, the rules governing application of wood ash in the forest are much more lax. Determination of heavy metal content in wood ash cannot be used to determine compliance with DüMV standards because of the high content and fluctuating nature of heavy metals found. Recommendations and perspectives Presently an alternative approach for classifying the ecologic risk of wood ash recycling is being developed.     

Nutzen und Grenzen von Modellökosystemen zur Erfassung der Wirkung von Schadstoffen auf benthische Lebensgemeinschaften

The aim of this short article is to deliver insight into the prospects and constraints of model ecosystems in the assessment of effects of contaminants on benthic communities, especially the meiofauna. Numerous substances accumulate in sediments and thus a complex contamination exists which is able to impair benthic organisms. Besides direct effects, indirect effects are of importance and should be considered. Changes in competition and predator-prey-relationships lead to a variety of effects in the benthic food web that cannot be explained by direct toxicity of contaminants. In this article two microcosm studies, one with the heavy metal cadmium and one with the veterinary pharmaceutical Ivermectin, are presented which both showed direct as well as indirect effects. A significant change in abundance and composition of the meiofauna community has been observed. The prediction of field-effects of a certain substance as well as the interpretation of field data are the aims of model ecosystems. Both studies proofed that the investigation of meiofauna in microcosms is a promising tool to achieve these aims. Furthermore, microcosms with meiofauna might be able to assist the effect-directed analysis (EDA) in a risk assessment by identifying the effect-causing contaminants of complex environmental samples.     

Konzentration und Verteilung von Platingruppenelementen (PGE) in Böden

In Germany automobile exhaust control by platinum-group element (PGE) loaded catalysts is now well established. By mechanical or chemical attrition, these catalysts could act as a potential source for a significant increase of PGE abundances in the environment. During a recent research program concentrations and distributions of PGE in different soils were measured along the highway A66 Frankfurt-Wiesbaden. 71 nearsurface soil samples were analysed by graphite furnace atomic absorption spectrometry (GFAAS). The results revealed average abundances of 10 ppb platinum and 3 ppb ruthenium. Palladium as well as rhodium were below the analytical detection limit of 2 ppb. With increasing distance from the highway edge PGE values gradually decreased. Only the upmost soil layer, down to 20 cm depth, contains measurable PGE concentrations. Results: There are irregular PGE concentrations of anthropogenic origin in different soils along the highway A66 Frankfurt-Wiesbaden possibly arising from automobile exhaust catalysts. Further monitoring could help to avoid potential environmental hazards. *** DIRECT SUPPORT *** A00HP020 00002     

Einsatz der Hasse-Diagrammtechnik zur vergleichenden Datenanalyse von Biomarkerantworten und ökotoxikologischen Tests

Ecotoxicological investigations focus on biological systems and their response to chemically induced stress. Experimental techniques are much more developed than deterministic dynamic modelling. In this methodological contribution a technique is presented, based on lattice theory. This technique, also calledHasse diagram technique, allows data analysis with respect to comparative evaluation. Hasse diagrams are used

? to suggest a possible measure of microbial diversity,

? to analyze dependencies between phospholipid fatty acids and simple geochemical parameters on an ordinal scale and

? to visualise complex results of interactions of humic substances with xenobiotics.

     

Bakterielle Mineralisierung von Dibenzofuran,Dibenzo-p-dioxin und 1,2,4,5-Tetrachlorbenzol in Böden

Quantitative degradation of dibenzofuran (DBF), dibenzo-p-dioxin (DBD), and 1,2,4,5-tetrachlorbenzene (TeCB) by the bacterial strains sphingomonas sp. HH 69, sphingomonas sp. RW1 and pseudomonas sp. PS 14 was investigated by radio-tracer techniques in liquid cultures and especially in samples of different soils. Pure strains converted DBF, DBD and TeCB up to 80% to CO₂ within few days. This degradation occurred not only in aerated liquid cultures but also in heterogeneous soils, with low levels of other pollutatns at favourable pH-values. Rate and degree of the mineralization of DBF, DBD and TeCB were independent of the DBF-, DBD-, or TeCB-concentration in the soils within a broad range. There was obviously a close correlation between the starting concentration of active cells (starting bacterial count) and the rate of degradation of the test substances in soil; by raising the starting bacterial count in the soil samples, mineralization of DBF, DBD, and TeCB, respectively, was clearly accelerated. However, under nearly the same coniditions in more acid soils (pH-values < 4) no significant degradation of DBF, DBD, and TeCB to CO₂ took place. As expected, this difficulty can be overcome by mixing CaCO₂ into the acid soils (liming). Easily utilizable substances like peptone, triolein, and glucose added as special carbon and energy sources to low contaminated soils, had only a small—if any—effect on the mineralization of DBF, DBD, and TeCB. In soils contaminated by a mixture of pollutants, the bacterial strains could develop their degradation capacity only to a limited extent and showed different degradation effects depending on the basic type of contamination. The efficiency of the degradation specialists was thus dependent on the “Chemical Environment” (type and concentration of contaminants present).     

Screening zur Mobilisierbarkeit von Kupfer aus Böden und Schlämmen

In the presented paper, the pHstat-procedure is compared with four simple extraction procedures. These procedures make use of the following extraction solutions: distilled water (based on DIN 38414 part 4, DIN-S4), 1 mol/L ammonium nitrate, 1 mol/L ammonium acetate at pH 7.0 and 1 mol/L sodium acetate at pH 5.0, respectively. The extraction procedures were applied to 10 different soil samples and 2 sludges. The DIN-S4-procedure shows the lowest amounts of mobilized copper. The highest parts are extracted by sodium acetate solution. For the procedures investigated, the mobilized amounts extracted by ammonium acetate solution are most comparable with those of the pHstat-procedure. The simple sodium acetate procedure is useful for screening to select significant samples from a large number of soils. Thus, the high effort caused by the pHstat-procedure can be reduced to a minimum through the application of this screening procedure. The application of this screening, however, is difficult for samples containing large parts of organic compounds, e.g. sewage sludge. These samples show a different extracting behaviour.     

Metallakkumulation in Moosen: Standörtliche und regionale Randbedingungen des Biomonitoring von Luftverunreinigungen

Goal and Scope

Several studies show that the concentration of metals in mosses depends not only on metal deposition but also on factors such as moss species, canopy drip, precipitation, altitude, distance to the sea and the analytical technique used. However, contrasting results have been reported and the interpretation of the spatial variability of the metal accumulation in mosses remains difficult. In the presented study existing monitoring data from the European Heavy Metals in Mosses Surveys together with surface data on precipitation, elevation and land use are statistically analysed to assess factors other than emissions that have an influence on the metal accumulation in the mosses.

Main Features

Inference statistics and Spearman correlation analysis were applied to examine the association of the metal accumulation and the distance of the monitoring sites to the sea as well as the altitude. Whether or not significant differences of the metal loads in the mosses exist at national borders was examined with help of the U-test after Mann and Whitney. In order to identify and rank the factors that are assumed to have an influence on the metal uptake of the mosses Classification and Regression Trees (CART) were applied.

Results

No clear tendency could be derived from the results of the inference statistical calculations and the correlation analyses with regard to the distance of the monitoring site to the sea and the altitude. According to the results of the CART-analyses mainly the moss species, potential emission sources around the monitoring sites, canopy drip and precipitation have an effect on the metal bioaccumulation. Assuming that each participating country followed strictly the manual for sampling and sample preparation the results of the inference statistical calculations furthermore suggest that in most cases different techniques for digestion and analysis bias the measurements significantly.

Discussion

For the first time a national monitoring data base consisting of measurement data and metadata as well as surface information on precipitation, land use and elevation was applied to examine influence factors on the metal bioaccumulation in mosses. The respective results mirror existing knowledge from other national studies to a large extend, although further analyses are necessary to affirm the findings. These analyses should include data from other national monitoring programmes and should additionally be carried out with other decision tree algorithms than CART.

Conclusions

The local variability in the metal concentration in mosses can be uncovered in terms of predictors or underlying hidden causes by using CART. Ideally, such an approach should be applied across the whole of Europe. This will only be feasible if all participating countries provide additional information about site characteristics as currently is done in for example the German moss surveys.

Recommendations

The UNECE Metals in Mosses Survey experimental protocol should be improved in order to reduce the observed influences, to enhance standardisation, and to strengthen the quality control. This implies the integration of sampling site describing metadata into the assessment. Furthermore, basis research is needed to test the hypothesis concerning moss species-specific accumulation of depositions.

Perspectives

Provided that the presented results hold true in further analyses correction factors should be applied on the moss data in order to get the depicted spatial patterns and temporal trends of metal bioaccumulation unbiased. Such factors should be calculated for natural landscape units or ecoregions that are homogeneous with regard to climate, vegetation and altitude.     

Identifikation geeigneter Nachweismöglichkeiten von hormonaktiven und reproduktionstoxischen Wirkungen in aquatischen Ökosystemen

Background and objectives There is an urgent need to detect, assess, and reduce effects of hormonally active compounds and endocrine disrupters in aquatic systems, as reflected in national research programs like the Swiss NRP 50 “Endocrine Disruptors” and its consensus platforms. As a medium-term measure, the EU strategy on endocrine disruptors (SEC(2007)1635) uses the Endocrine Disruptor Testing and Assessment (EDTA) Task Force of the Organisation for Economic Co-operation and Development (OECD) along with other research activities. In particular the test methods of the OECD that are currently in validation or already validated may contribute to a better understanding of the extent of endocrine disruption, in particular if they are applied on environmental samples and in the context of risk-assessment strategies, for instance in waste water treatment. This article aims to give an overview and an evaluation on available and validated biological test systems for the detection of endocrine disruptive and reproductive effects in aquatic systems. Based on this a recommendation for a modular ecotoxicological test platform is given. The study focuses on test methods for sex hormone active substances. Material and methods On the basis of an extensive literature search and ongoing international validation efforts by the OECD for methods to detect endocrine disruptive effects, 15 biological test methods (5 in vivo and 10 in vitro) were selected. Comprising, for example, of eight OECD methods and three out of five in-vitro methods mentioned in the Global Water Research Coalition (GWRC) report “Tools to detect estrogenic activity in environmental waters” (Leusch 2008). Experienced users and developers were then asked to rate the test according to given relevant criteria. The resulting criteria profiles were compiled, compared, and evaluated. Results The methods were selected on the basis of validation status, distribution, their suitability for standardisation, and their proven sensitivity for environmental samples. We assumed that specific YES/YAS-procedures and the ER/AR Calux systems achieve the mentioned criteria. In the case that strong cytotoxicity of environmental samples affects the applicability of cellular reporter gene assays, alternatively a molecular receptor binding assay (e.?g. ELRA) could be used. Additional molecular receptor binding assays are currently in validation by the OECD. Modulating effects on steroidgenesis and probably even on aromatase activity can be detected by the OECD validated H295R Steroidgenesis Assay. Its applicability for environmental samples is currently tested. As in-vivo methods different fish assays (e.?g. Fish Screening Assay and the Zebrafish Embryo Test) were evaluated. For the detection of effects on the thyroid-associated hormone system the Amphibian Metamorphosis Assay (XEMA, for Xenopus Metamorphosis Assay) will soon be available as an OECD-Guideline. Because of trends in the international community to avoid tests with vertebrates in the future, only an optional recommendation was given for such tests. Reproduction toxicity, on the other hand, can be sensitively tested by the tests using the gastropod Potamopyrgus antipodarum, which, as invertebrate tests, do not require a permit to perform animal testing procedures. This test is also sensitive for many substances which are able to induce an endocrine disruption in vertebrates. Its validation is currently performed by the OECD. The applicability of the herein proposed test methods of the modular test platform for environmental-sample assessments were previously confirmed by several published scientific studies. Discussion We propose several test methods to be included in an application-oriented modular test platform. These tests should be suitable to efficiently indicate different mechanisms of endocrine disruption and reproduction toxicity, and may be employed in different situations according to their respective advantages. The modular test platform is able to detect impacts on the reproduction relevant effects in invertebrates, amphibians, and fishes. Furthermore the different mode of actions of estrogenic and androgenic receptor binding, steroid genesis and perhaps even the modulation of aromatase are detectable. A research report on the relevance of endocrine substances and pharmaceuticals in aquatic environments (Moltmann et al. 2007) showed that endocrine end-points of in-vivo tests (31 of 71 tested substances) tend to be more sensitive than general ecotoxicological end-points, such as mortality and growth. Consequently, endocrine and general-toxic effects should be detected in an integrative manner by ecotoxicological test platforms. Conclusions By combining literature research with a targeted query for information about the chosen test procedures it was possible to obtain a detailed overview about the current state-of-the-art of science and technology in the detection of hormone-active effects and reproduction toxicity. Ecotoxicological and regulative aspects were considered equally, and the applicability of the test procedures was evaluated. Because of the diversity of endocrine disrupting mechanisms, a modular combination of in-vivo and in-vitro methods in a joint test platform is needed to recognise and differentiate the transitions from hormone-active effects to endocrine disruption. Recommendations and perspectives Our study leads to a proposal of a modular ecotoxicological test platform, which offers an integrative detection of hormone-active and reproduction-relevant effects in the aquatic environment. The modular system presented here allows the switching between test modules according to the continuously developing state-of-the-art of science and technology as well as the incorporation of novel developments. Further standardisation of such methods for regulative applications is recommended.     

Untersuchungen zum ökotoxikologischen Schädigungspotenzial und Erosionsrisiko von kontaminierten Sedimenten in staugeregelten Flüssen

Particle-bound pollutants accumulate in river-bottom sediments, a process which results in a significant decrease in the ecotoxicological availability of toxicants for the majority of aquatic organisms. Under normal hydrologic conditions, the release of contaminants from bottom sediments is usually of minor importance. In contrast, flood events may remobilize highly contaminated sediments via in-stream erosion. The objective of this study was to develop a combined ecotoxicological and hydraulic approach to elucidate the ecotoxicological implications associated with the risk of erosion of contaminated sediments. This integrated strategy was applied to the lock-regulated Neckar river in Southern Germany. Both the bottom-sediment cores and suspended matter from two intensive flood events were investigated. Sediment samples below an erosional unconformity showed a sharp increase in the ecotoxicological load. Moreover, it was found that major flood events (HQ₅ and higher) could possibly erode even very old, well-consolidated and highlycontaminated sediments. The suspended matter of the high discharge events investigated (return periods of 15 to 20 years) exerted significantly higher cytotoxicity and mutagenicity than a moderate flood with a 1-year return period. These findings support the conclusion that the observed ecotoxicological effects during major floods may at least in part be due to the in-stream erosion of highly contaminated bottom sediments.     

Die Rolle von Stressproteinen bei der Anpassung an Umweltbedingungen: Ökophysiologische, ökotoxikologische und evolutionsbiologische Implikationen

Introduction Stress proteins are ubiquitous and inducible by a vast number of stressors. Their relevance for ecological and microevolutionary adaptations has been investigated since the early 1990s. Stress proteins: function and induction Stress proteins are categorized in different families of which most data have been recorded for Hsp70, Hsp60, and Hsp90. These cytoplasmatic proteins play a central role in protein folding, the trans-membrane passage of proteins, and the stabilization of signal transduction pathways. Hsp70 is induced by elevated concentrations of unfolded or malfolded intracellular proteins and hence indicates proteotoxicity. Modulation of the stress protein level by internal factors During certain developmental stages, stress protein levels are modified by internal factors. This has been particularly shown for the early ontogeny, for the formation of quiescent stages, and for the reproductive phase. Adaptation to external stressors in the laboratory Laboratory studies on the effects of ecophysiologically relevant stressors have revealed short-term physiological adaptation to heat or environmental chemicals to involve an elevation of stress protein levels. Extreme stressor impact disrupts this responsive mechanism. Adaptation to field situations Also in the field, physiological responses to temperature fluctuations or contamination usually involve the induction of stress proteins which can be used as a biomarker of proteotoxic effect. Implications for evolutionary biology In long-term contaminated sites, however, phenotypes displaying low stress protein levels appear to be evolutionarily favored. Whether different stress response strategies may entail different degrees of phenotypic variation even under natural conditions – via Hsp action on developmental canalization – is a matter of hot debate.     

Die ökologische Bewertung von Seeufern in Deutschland

Goal and Scope

Details about the ecological function of lake shores as ecotones between land and lakes are not well-known. These ecotones are also heavily exploited and, in part, considerably changed. Whereas anthropogenic nutrient loading is decreasing, structural changes are increasing. Unfortunately, there is a deficit in methods of evaluation and decision processes.

Main Focus

Even the EU-water framework directive was no remedy for this deficit, as lake shores were included only implicitly. In this article several evaluation methods and their conceptual groundwork are presented. However, these methods were not developed for lake shore research. Therefore, criteria are proposed which could fulfill the specific demands of lake shore assessments. The management of lakes shores should consider structural and biological parameters, and be agreeable to local residents.

Results and Conclusions

In addition to conventional biodiversity methods, the ecology of lake shores could also be represented by a functional food net, for example in benthic invertebrates. But even quantification of biodiversity alone creates many problems. A simple biodiversity index cannot meet all the demands placed on a method of evaluation in complex situations, especially when coupled with additional information on structure, practicability, costs, etc. For these reasons, assessments for future management cannot be based on such an index.

Outlook

A possible approach to include this complexity in assessments is to apply mathematical models and theoretical order concepts.     

Die Lösung von Nutzungskonflikten aufgrund flächenhafter Bodenkontaminationen mit Hilfe Geografischer Informationssysteme und Mitteln der Geostatistik

Goal and Scope

By means of Geographical Information Systems (GIS) and other geostatistical methods, the extensive heterogeneous data materials of large area soil contaminations are re-evaluated to eliminate exploitation conflicts and investment obstacles.

Methods

The mapping of relevant frame criteria generates the basis to solve the conflicts, such as types of exploitation of field areas, development plans, protected areas, flooded areas, etc. as well as their geo-referencing in the Geographical Information System. The critical examination of data age and quality as well as the geostatistical evaluation by means of semivariogram analysis and Kriging technique are essential prerequisites for the determination of areas related to utility and exceeding test values.

Results and Conclusions

As a result, one can modify the hazardous situation and can provide recommendations for an optimal elimination of hazards, mostly in the framework of scheduled measures in terms of town planning.

Outlook

On the basis of the results obtained, further exploitation or monitoring work can clearly be optimized.     

Bestimmung von Redoxzonen in einem mineralölbelasteten Grundwasserleiter

Goal and Scope

The goal of this study is the investigation and the grafic presentation of the characteristic redox zonation in a mineral oil contaminated aquifer which will be formed in the plume downstream of the contamination source. Methanogenic conditions, sulfate-reduction, Fe(III)-reduction, Mn(IV)-reduction, nitrate-reduction, aerobic conditions. By that indications type and degree of microbial degradation which is the most important part in Natural Attenuation (NA) processes can be obtained easily.

Methods

Changes of the groundwater parameters Eh, O₂, NO ₃ ^? , SO₄ ^2?, Fe²⁺, Mn²⁺, HCO₃ ^?, Ca²⁺ will be measured upstream, downstream and also in the centre of the plume. The results will be presented in a sequence of special diagrams.

Results and Conclusion

When microbial degradation of hydrocarbons takes place, a microbial community will always use that electron acceptor from which it will gain a maximum of energy by the corresponding redox-reactions. This means as long as oxygen is available this will be used. After its depletion nitrate serves as electron acceptor leading via nitrite to the formation of nitrogen or ammonia. Manganese (IV) and Iron (III) species which are rather insoluble are mainly available from the soil-phase, can act as electron acceptor as next, leading to soluble Manganese (II) and Iron (II) compounds in groundwater. Finally before methanogenic conditions occur sulphate will become a suitable electron acceptor leading to the formation of hydrogen sulphide. All these processes of mineralization of the hydrocarbons will lead to the production of CO₂ and as consequence to an increase of HCO₃ ^? in groundwater changing the calcareous/carbonic acid-equilibrium. By that more soluble Ca(HCO₃)₂ is formed from insoluble CaCO₃, so the concentration from Ca²⁺ will also inerease. Thus, by the action of microorganisms, a typical redox-zonation and changes of other parameters will occur.

Recommendations and Perspective

To follow the changes in time and space of some characteristic groundwater parameters is a simple way to estimate the potential of microbial degradation in a contaminated aquifer considering Natural Attenuation (NA)-processes.     

Ökotoxikologische und humantoxikologische Risikobewertung PAK-belasteter Böden vor und nach biologischer Behandlung

The goal of the present work is to assess the adverse effects of soil bound polycyclic aromatic hydrocarbons (PAH) which remain in soils after biological remediation. We focus on risk assessment for mammalian species with respect to the oral uptake of contaminated soil particles and compare the results of a biomarker test with those of an ecotoxicological assay, the bioluminescence inhibition test withVibrio fischeri. As a biomarker effect in mammals, we determined the liver microsomal cytochrome P450 enzyme CYP1A1 which is induced by PAH in exposed rats. After biological soil treatment, different amounts of PAH remain in the soil depending on the soil properties and initial pollutant composition. Particularly, higher condensated PAH resists biological treatment due to its hydrophobicity. In addition, high amounts of organic carbon in the soils affect remediation efficiency. In the bioluminescence inhibition test, eluates of all biologically treated soils studied do not reveal any or only low inhibitory effects. In contrast, the oral uptake of biologically treated contaminated soils leads to induction levels for CYP1A1 similar to those in the untreated samples. A good correlation is obtained between CYP1A1 levels and the amount of 5 and 6-ring PAH in the soil samples. The main result is that the remediation efficiency determined by the luminescence test is not reflected by the biomarker test, a finding which indicates the high bioavailability of residual PAH in soils. Consequently, new criteria for human risk assessment can be delineated.     

Existierende dioxininventare weltweit und neue methodik zur erstellung von vergleichbaren und vollständigen emissionsinventaren

Presently, there are less than 20 inventories to estimate releases of dioxins and furans. According to a recent survey, 12,900 g TEQ of these unwanted byproducts are emitted into the atmosphere by 16 countries. Highest emissions are from the densely populated industrialized countries of the Northern hemisphere — Japan and the United States of America. Whereas the metal producing and recycling industry is the sector with the highest dioxin emissions in Europe, waste incineration is considered to be the major source in many other countries. Measures to reduce dioxin emissions have resulted in strong downward trends, as shown e.g. in Germany and Japan; however, potentials for further reduction have been identified. So far, countries have utilized own methods to calculate their dioxin emissions and the majority has addressed releases to air only. The future Stockholm Convention on POPs will require to continuously reduce dioxin emissions. In order to assist countries in inventory making, UNEP has produced the Toolkit, a methodology to establish comparable dioxin inventories that address releases to air, water and land, with products and in residues.     

Ökologische Optimierungspotenziale der energetischen Nutzung von Palmöl

Aim and Background The use of palm oil for bioenergy has become increasingly important for Europe in the last years because of its favourable proportion of yield to area under cultivation. Thus, palm oil presents a low-priced alternative to other energy sources, e.?g. rapeseed oil. Currently, however, palm oil gets a bad press due to new studies about the negative environmental consequences of cultivation practices. Due to the high demand for palm oil, land is becoming scarce. This results in the clearing of primary forests and consequently in the loss of biodiversity and in an increase of greenhouse gas emissions. To reduce the latter, not only the process of oil palm cultivation has to be optimised but also the practice of establishing new plantations by clearing natural forests has to be questioned. The aim of this article is to disclose potentials for greenhouse gas reductions in existing as well as in newly-planned oil palm plantations. Results and Discussion For existing oil palm plantations, two main fields for possible optimisation can be identified: one is improving the plantation management, the other is increasing the efficiency of the utilisation of waste products such as fibres and husks or oil mill effluents. For newly-planned oil palm plantations alternative land use scenarios have to be considered. The results show a big potential for optimisation. Thus, the greenhouse gas balance improves slightly if plantations are run more efficiently. If the waste products are used to generate energy, there are significantly positive effects on the greenhouse gas balance, especially through the reduction of methane emissions. By running a plantation in a professional best-practice way, 4.8 t of greenhouse gases can be saved annually per hectare cultivation area, expressed as CO₂ equivalents. If newly-planned oil palm plantations are established on fallow land, greenhouse gas emissions can be further reduced by an additional 4.8 t of CO₂ equivalents per hectare and year. From an economic perspective, this may be more costly than clearing primary forest but it is advantageous for both the greenhouse gas balance and the biodiversity of the concerned areas. All in all, exploiting the whole potential for optimisation could result in the saving of 10.2 t CO₂ equivalents per hectare and year more than it is the case in the existing mode of cultivation. Conclusions and Perspectives Due to the high demand of palm oil by the world market, cultivation areas for oil palms are becoming increasingly scarce. Thus, it is vital to exploit the full potential of oil palm cultivation in an environmentally and economically sustainable way. The management of plantations has to be optimised and a generally valid waste management system must be implemented in existing and future plantations. New plantations should preferably be established on fallow land, not by the clearing of primary forests. It is essential for a sustainable palm oil production to tap the full potential for optimisation. This, however, is currently not happening due to the high start-up investments. It is thus recommended to introduce an internationally valid certification system which may provide an incentive for more sustainable and effective production methods.