Comprehensive Analysis of Radioactive and Chemical Contamination of Water Bodies in the Area of Radium-Industry Waste Storage

Genotoxicity and cytotoxicity of water samples from the creeks flowing through the area of radium mining waste storage into the Ukhta River and from this river were assessed using biological tests with Allium schoenoprasum L. Chemical analysis of the samples indicated that concentrations of parent heavy natural radionuclides, ²¹⁰Pb, and ²¹⁰Po do not exceed the allowable level. Of ten heavy metals, only Zn and Mn concentrations exceeded MACs. The results of biological tests provided evidence for the significantly increased genotoxicity of water samples. The mitotic index increased with an increase in the concentration of Zn ions and manifested an inverse dependence on the activity of ²³⁸U. The genotoxic effect linearly depended on Zn concentration. Possible mechanisms responsible for the induction of these biological effects are discussed.     

Radiological hazards of TENORM in the wasted petroleum pipes

Disposal petroleum pipes containing sludge and scale as a technically enhanced natural occurring radioactive material (TENORM) leads to internal and external radiation hazards and then a significant radiation dose to the workers. In order to contribute to a future waste management policy related to the presence of TENORM in the disposal sites of wasted petroleum pipes, scale and sludge as TENORM wastes are collected form these disposal pipes for radiometric analysis. These pipes are imported from onshore oilfields at south Sinai governorate, Egypt. The highest mean ²²⁶Ra and ²²⁸Ra concentrations of 519 and 50 kBq/kg respectively, were measured in scale samples. Sludge lies within the normal range of radium concentration. The average absorbed dose caused by the exposure to the wasted pipes equal to 4.09 μGy h⁻¹ from sludge and 262 μGy h⁻¹ from scale. This is much higher than the acceptable level of 0.059 μGy h⁻¹. Due to radon inhalation, important radon related parameters are calculated which advantage in internal dose calculation. Fairly good correlation between real radium content and radon exhalation rate for sludge samples is obtained. The hazards from sludge come from its high emanation power for radon which equal to 3.83%. The obtained results demonstrate the need of screening oil residues for their radionuclide content in order to decide about their final disposal.     

Occurrence and distribution of organochlorine pesticides - lindane, p,p'-DDT, and heptachlor epoxide - in surface water of China

Persistent organochlorine pesticides pollutants (OCPs) have been reported to occur at relatively high concentrations in some Chinese waters. In order to map the distribution of organochlorine pesticides in the surface water throughout China, samples were collected from over 600 sites in seven major river basins and three main internal rivers drainage areas during 2003 and 2004. The surface water samples were analyzed for the representative organochlorine pesticides contaminants including lindane (gamma-HCH), p,p'-DDT and heptachlor epoxide. In general, the most frequently detected compound was lindane, being detected in 83.9% of samples (mean=31.3 ng/l; range <0.17-860 ng/l), and the highest concentration was present in the Yellow River basin. p,p'-DDT was detected in 63.1% of the samples collected (mean=14.6 ng/l; range <0.14-368 ng/l) with the highest concentration present in the Huaihe River basin. Heptachlor epoxide was detected in only 9.3% of water samples (range <0.11-10 ng/l). Measured concentrations for the three compounds were low and rarely exceed the environment quality standard for surface water of China. Lindane was more frequently detected at much higher concentrations in the rivers of northern China compared with those of southern China. The sites with higher concentration of lindane and p,p'-DDT mainly occurred in the Yellow River and Huaihe River basins, so the results of this investigation indicate that the organochlorine pesticide contamination of Yellow River and Huaihe River basins should be of particular concern relative to the other basins. When compared with other regions of the world, it appears that the Chinese surface water is moderately polluted by lindane and p,p'-DDT.     

A bioassay approach to determine the dioxin-like activity in sediment extracts from the Danube River: ethoxyresorufin-O-deethylase induction in gill filaments and liver of three-spined sticklebacks (Gasterosteus aculeatus L.)

Sediment samples from the upper Danube River in Germany have previously been characterized as ecotoxicologically hazardous and contaminants in these sediments may contribute to the observed decline of fish populations in this river section. For the investigation of sediment toxicity there is a need for development, standardization and implementation of in vivo test systems using vertebrates. Therefore, the main objective of this study was to apply and evaluate a recently established fish gill EROD assay as a biomarker in sediment toxicity assessment by using extracts of well characterised sediment samples from the upper Danube River. This to our knowledge is the first application of this novel assay to sediment extracts. Sediments from four different sites along the upper Danube River were Soxhlet-extracted with acetone and dissolved in DMSO. Three-spined sticklebacks (Gasterosteus aculeatus L.) were exposed for 48 h to various concentrations of the extracts, to the positive control beta-naphthoflavone or to the solvent. Measurements of EROD activity in gill filaments and liver microsomes followed the exposure. Concentration-dependent induction of EROD in both gill and liver was found for all sediment extracts. The highest EROD-inducing potency was determined for extracts of sediments from the sites "Opfinger See" and "Sigmaringen" and the EROD activities in gill and liver correlated well. The results from the gill and liver assays were in accordance with in vitro results of previous investigations. The EROD activities measured in the present study corresponded with the concentrations of PAHs, PCBs and PCDD/Fs in the sediment samples derived in a previous study. The sticklebacks in this study were in the reproductive phase and a stronger EROD induction was obtained in the females than in the males. Implementation of the EROD assay in testing of sediment extracts gave highly reliable results which make this assay an ecotoxicologically relevant method for assessment of contamination with Ah receptor agonists in sediments.     

Trends in the levels of halogenated flame retardants in the Great Lakes atmosphere over the period 2005–2013

Air (vapor and particle phase) samples were collected every 12 days at five sites near the North American Great Lakes from 1 January 2005 to 31 December 2013 as a part of the Integrated Atmospheric Deposition Network (IADN). The concentrations of 35 polybrominated diphenyl ethers (PBDEs) and eight other halogenated flame retardants were measured in each of the ~ 1,300 samples. The levels of almost all of these flame retardants, except for pentabromoethylbenzene (PBEB), hexabromobenzene (HBB), and Dechlorane Plus (DP), were significantly higher in Chicago, Cleveland, and Sturgeon Point. The concentrations of PBEB and HBB were relatively high at Eagle Harbor and Sturgeon Point, respectively, and the concentrations of DP were relatively high at Cleveland and Sturgeon Point, the two sites closest to this compound's production site. The data were analyzed using a multiple linear regression model to determine significant temporal trends in these atmospheric concentrations. The concentrations of PBDEs were decreasing at the urban sites, Chicago and Cleveland, but were generally unchanging at the remote sites, Sleeping Bear Dunes and Eagle Harbor. The concentrations of PBEB were decreasing at almost all sites except for Eagle Harbor, where the highest PBEB levels were observed. HBB concentrations were decreasing at all sites except for Sturgeon Point, where HBB levels were the highest. DP concentrations were increasing with doubling times of 3–9 years at all sites except those closest to its source (Cleveland and Sturgeon Point). The levels of 1,2-bis(2,4,6-tribromophenoxy)ethane (TBE) were unchanging at the urban sites, Chicago and Cleveland, but decreasing at the suburban and remote sites, Sturgeon Point and Eagle Harbor. The atmospheric concentrations of 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EHTBB) and bis(2-ethylhexyl)-tetrabromophthalate (BEHTBP) were increasing at almost every site with doubling times of 3–6 years.     

Environment and sustainability of the Middle Route,South-to-North Water Transfer Project in China: a close look

In many arid and semiarid regions, water scarcity, population increase and frequent droughts are exerting great pressures on water resources. Presently, the Mid-route of the South-to-North Water Transfer Project (MRSN) was built to mitigate the water crisis in the north of China by long-distance transfer of water from the Yangtze River in southern China. This study investigated the running condition of the MRSN, including operation management, freezing situation and water quality. Water samples were also taken from different sites and then analyzed in laboratory. Results suggest that the project was reasonably designed and the project management was excellent. Closed management was adopted in the project to protect water quality. The sediment concentrations and water turbidities of the water samples were in the range of 0.2–0.8 kg/m³ and 0.8–1.7 NTU, respectively, which met or were close to the standards of drinking water in China. Water freezing is also not a problem, since the thickest ice was only 0.9 cm even in the coldest season as the authors measured the investigation, and at the same time, the ice booms worked well. In the future, it is promising that to effectively integrate the methods of self-rescuing and water importing could fundamentally conquer water shortage, reasonably allocate water resources and finally achieve the harmonious development of economics, ecology and society.     

Vertical distribution of anthropogenic radionuclides in cores from contaminated floodplains of the Yenisey River

The Mining and Chemical Industrial Combine, Zheleznogorsk (MCIC, previously known as Krasnoyarsk-26) on the River Yenisey has contaminated the surrounding environment with anthropogenic radionuclides as a result of discharges of radioactive wastes. The purpose of this study was to investigate the vertical distribution of anthropogenic contamination (¹³⁷Cs and plutonium) within floodplain areas at different distances from the discharge point. Sites were chosen that display different characteristics with respect to periodic inundation with river water. Cs-137 activity concentrations were in the range 23–3770 Bq/kg (dry weight, d.w.); Pu-239,240 activity concentrations were in the range <0.01–14.2 Bq/kg (d.w.). Numerous sample cores exhibited sub-surface maxima which may be related to the historical discharges from the MCIC. Possible evidence indicating the deposition of earlier discharges at MCIC in deeper core layers was observed in the ²³⁸Pu:^239,240Pu activity ratio data: a Pu signal discernible from global fallout could be observed in numerous samples. Cs-137 and Pu-239,240 activity concentrations were correlated with the silt fraction (% by mass <63 μm) though no significant correlation was observed between (grain-size) normalised ¹³⁷Cs activity concentrations and distance downstream from the MCIC.     

Natural radioactivity in winter wheat from organic and conventional agricultural systems

The distribution of natural radionuclides was studied in winter wheat plants collected from three sites in Belgium during 2004-2007. Activity concentrations of ⁴⁰K, ²²⁶Ra, ²²⁸Ra and ²²⁸Th in organically and conventionally grown wheat, and in the corresponding soil samples, were determined using ultra low-level gamma-ray spectrometry. The observed soil-to-wheat concentration ratios were calculated for the different parts of the wheat plant (root, stem and grain) in the two agricultural systems (organic and conventional). There were large variations in radionuclide activity concentrations between the sites and fields, but no significant difference between conventionally and organically grown wheat plants was observed.     

Assessment of the radiological impact of oil refining industry

The field of radiation protection and corresponding national and international regulations has evolved to ensure safety in the use of radioactive materials. Oil and gas production processing operations have been known to cause naturally occurring radioactive materials (NORMs) to accumulate at elevated concentrations as by-product waste streams. A comprehensive radiological study on the oil refining industry in Egypt was carried out to assess the radiological impact of this industry on the workers. Scales, sludge, water and crude oil samples were collected at each stage of the refining process. The activity concentration of ²²⁶Ra, ²³²Th and ⁴⁰K were determined using high-resolution gamma spectrometry. The average activity concentrations of the determined isotopes are lower than the IAEA exempt activity levels for NORM isotopes. Different exposure scenarios were studied. The average annual effective dose for workers due to direct exposure to gamma radiation and dust inhalation found to be 0.6 μSv and 3.2 mSv, respectively. Based on the ALARA principle, the results indicate that special care must be taken during cleaning operations in order to reduce the personnel's exposure due to maintenance as well as to avoid contamination of the environment.     

The importance of physico-chemical parameters on the speciation of natural radionuclides in riverine ecosystems

External gamma radiation levels were measured in the catchment areas of the Sharavathi River and the dose rates in air were found to be in the range 26.0-61.0 nGy h⁻¹. Soil and sediment samples of the riverine environment were analysed for natural radionuclides such as ²²⁶Ra, ²³²Th and ⁴⁰K using a NaI(Tl) gamma spectrometer. The activity concentration of ²¹⁰Pb and ²¹⁰Po in soil and sediment samples was determined by radiochemical separation techniques. Evaluation of the activity concentration of radionuclides with grain size revealed an increase in the activity of ²²⁶Ra, ²³²Th and ⁴⁰K towards fine grain size. The activity concentrations for all isotopes in all samples were not significantly correlated with pH. However, the activity of ²¹⁰Po and ²¹⁰Pb in sediment showed a moderate positive correlation with organic matter content and a good correlation with clay content of sediment.     

Monitoring and assessment of radionuclide discharges from Temelín Nuclear Power Plant into the Vltava River (Czech Republic)

The paper summarizes impacts of the Temelín Nuclear Power Plant (NPP) on the Vltava and Labe River basins. The study is based on the results of long-term monitoring carried out before the plant operation (1989–2000), and subsequently during the plant operation (2001–2005). In the first period, the main objective was to determine background radionuclide levels remaining in the environment after global fallout and due to the Chernobyl accident. A decrease in the concentrations of ⁹⁰Sr, ¹³⁴Cs and ¹³⁷Cs, which was observed before the plant operation, continued also during the subsequent period. Apart from tritium, the results of the observation did not indicate any impacts of the plant on the concentrations of activation and fission products in the hydrosphere. The annual average tritium concentrations in the Vltava River were in agreement with predicted values. The maximum annual average tritium concentration (13.5 Bq L⁻¹) was observed in 2004 downstream from the wastewater discharge in the Vltava River at Solenice. Estimated radiation doses for adults due to intakes of river water as drinking water contaminated by tritium are below 0.1 μSv y⁻¹.     

The speciation and bioavailability of mercury in sediments of Haihe River, China

Twenty-one sediment samples in 11 sites along the Haihe River and Dagu Drainage River, Tianjin, China, were analyzed to investigate the pollution status and bioavailability of mercury (Hg). The results showed that the Haihe River was slightly polluted with Hg when flowing through Tianjin city. On the contrary, the sediments collected from Dagu Drainage River, an important drainage river in Tianjin, were found to have very high Hg concentrations and the highest concentration reached 8779.1 ng g(-1) (dry weight). The methylmercury (MeHg) concentrations accounted for 0.1-2.4% (average: 0.9%) of total mercury (HgT) and were strongly influenced by HgT (r=0.91, p=0.99, n=20) and total organic carbon (TOC; r=0.76, p=0.99, n=20) contents in sediments. Moreover, a five-step sequential selective extraction (SSE) procedure was used to study the bioavailability of Hg in sediments. The mercury in sediments existed mainly as element Hg and mercury sulfide, which accounted for 46.5% and 39.0% of HgT, respectively. The percentage of exchangeable Hg (defined as water soluble Hg plus 'human stomach acid' soluble Hg) was only 0.1-4.6%. The distribution of exchangeable Hg showed an obvious difference to that of HgT, indicating that the HgT concentrations were absolutely insufficient to evaluate the risk of Hg in sediments.     

Endocrine and immunological parameters in individuals involved in Prestige spill cleanup tasks seven years after the exposure

In November 2002 the oil tanker Prestige spilled 63,000 tonnes of heavy oil off the northwest coast of Spain, impacting more than 1000 km of coastline. A general concern led to a huge mobilization of human and technical resources, and more than 300,000 people participated in cleanup activities, which lasted up to 10 months. Some endocrine and immunological alterations were reported in Prestige oil exposed subjects for several months. Therefore, the objective of this study was to evaluate if these alterations are still present seven years after the exposure. Fifty-four individuals exposed for at least 2 months were compared to 50 matched referents. Prolactin and cortisol plasma concentrations, percentages of lymphocyte subsets (CD3⁺, CD4⁺, CD8⁺, CD19⁺, and CD56⁺16⁺), plasma levels of circulating cytokines (interleukin (IL) 2, IL4, IL6, IL10, tumour necrosis factor α, and interferon γ), and serum concentrations of neopterin, tryptophan and kynurenine were determined in peripheral blood samples. Results showed significant differences in exposed individuals vs. referents only in cortisol (increase), kynurenine and %CD16⁺56⁺ lymphocytes (both decrease). Time of exposure to the oil or using protective clothes did not influence the results, but effect of using protective mask was observed on neopterin, %CD8⁺, CD4⁺/CD8⁺ ratio and IL4. Surveillance of the exposed individuals for early detection of possible health problems related to the endocrine or immunological systems is recommended.     

Chironomid larval deformity frequencies, mortality, and diversity in heavy-metal contaminated sediments of a Canadian riverine wetland

Sediment sampling at point source locations in the Niagara River watershed revealed an area in a Class One Provincially Significant Riverine Wetland that was located near the discharge of a stainless steel company. The site had unusually high concentrations of heavy metals and oily wastes in its sediments. The impacts of these oil and heavy metal contaminated sediments on an invertebrate population of midge (chironomid) larvae was assessed using both lab and field techniques. The midge larvae were the dominant invertebrates in this section of the river. Twenty-six percent of the chironomids from sites located 10 to 800 m downstream of the stainless steel company's point source were deformed. A lab study was carried out to determine what percentage of the observed deformities could be attributed to the heavy metal content of the sediments and what percentage was due to the organic fraction. Sediments collected near the point source were tested and found to be acutely toxic. Sediments collected 60 m downstream of the point source were teratogenic. The frequency of mentum deformities for chironomids reared in clean sediments (control aquaria) was only 2.2%. A similar deformity frequency (2.2%) was observed at the clean sediment reference site in the Welland River watershed. Of the 97 chironomids removed from the de-oiled heavy metal contaminated sediments in the test aquarium, 10.3% displayed deformities. This is believed to be one of the few times that chironomid deformities were induced in a controlled lab study in which heavy metal concentrations similar to those found in the field were used.     

Chemical and microbiological examination of well and Nile water

The chemical composition and microbiological contamination of well water and Nile River water used for drinking were investigated in localities around Khartoum, Sudan, to present baseline data. The chemical analyses results obtained indicated that public health hazards due to pH, Ca⁺⁺, CO₃^??, HCO₃^?, and NO₃^? are unlikely in all the samples studied, while Na⁺ and Cl^? ions concentrations in well water warrant some attention. Contamination from organic matter and suspended material is also negligible. Microbial contamination with coliform and fecal coliform is high in surface wells and in the Nile River, but negligible in deep bores, tap water, and mineral water. The logarithm of the colony count ranges from zero for mineral water to 6.8 for water from the White Nile. In addition to drawing further attention to the well water and Nile water used for drinking by a wide sector of the population, the microbial count data for water from the Nile disagreed with the generally held belief that the Nile, being one of the largest rivers of the world, carries no detectable fecal contamination. Our data also did not support the belief that the White Nile is more contaminated than the Blue Nile.     

A study of radionuclides, metals and stable lead isotope ratios in sediments and soils in the vicinity of natural U-mineralisation areas in the Northern Territory

Australian guidelines recommend that tailings materials from uranium (U) mining and milling be contained without any detrimental impact on the environment for at least 1000 years. Natural analogue sites are being investigated to determine if they can provide data on the rates of natural erosion processes which occur over these timescales, for input into predictive geomorphic computer models. This paper presents radionuclide, metal and stable lead (Pb) isotope data from sediment cores and surface soils in the vicinity of two mineralised areas in the Alligator Rivers Region.Surface scrapes from the natural Anomaly #2, south of the Ranger mineral lease, exhibit radiogenic ²⁰⁶Pb/²⁰⁷Pb and ²⁰⁸Pb/²⁰⁷Pb ratios, and elevated U and metal concentrations typical for a near surface U anomaly. In contrast, samples taken from the Koongarra mineral lease (KML) show radionuclide activity and metal concentrations similar to natural areas elsewhere in the Alligator Rivers Region and Pb isotope ratios are closer to present day average crustal ratios (PDAC), as the orebodies at KML are covered by surficial sand. A sediment core collected from Anbangbang Billabong, downstream of KML, exhibits small variations in Pb isotope ratios that indicate that approximately 1% of the upper sediments in the sediment core may be derived from material originating from the U anomaly at Koongarra.     

Study of total organic halogen as a means to detect groundwater contamination

Total organic halogen (TOX) was evaluated as a means to detect contamination of groundwater by halogenated organics. Groundwater samples from monitoring wells at 10 Canadian landfill sites were analyzed for carbon adsorbable TOX, total organic carbon and volatile organics. Low concentrations of TOX (range, ND-44; median 8 μg Cl L^?1) were found at nine sites, and elevated TOX levels (?2072 μg Cl L^?1) at the tenth site. This latter site was studied in greater detail, and TOX and GC-MS analyses were carried out for volatile, base neutral, and acid extractable fractions of the water in six monitoring wells at this site. The TOX and GC-MS techniques were found to provide complementary information. GC-MS analysis detected high concentrations of 1,2-dichloroethylene, trichloroethylene, toluene, and ethylbenzene in some of the wells. TOX measurements, however, indicated the presence of halogenated contaminants in the base-neutral and acid extractable fractions, which had not been detected by GC-MS.     

Seasonal and inter-annual variation of Beryllium-7 deposition in birch-tree leaves and grass in the northeast upland area of the Czech Republic

The activity concentrations of Beryllium-7 (⁷Be), a naturally occurring radioisotope produced in the atmosphere, were measured in leaves of birch-trees, above-ground parts of grass, soil and rainwater in the mountain massive Kralicky Sneznik (the northeast of the Czech Republic, altitude about 750 m) in the years of 2005, 2006 and 2007. Dried and ground samples of the plants and soils, and water samples from wet deposition were used to determine the ⁷Be content using a semiconductor gamma spectrometer. The ⁷Be values ranged from 147.0 to 279.6 Bq kg⁻¹, from 48.7 to 740.8 Bq kg⁻¹, from 2.1 to 8.7 Bq kg⁻¹, and from 0.6 to 1.9 Bq kg⁻¹ in birch-tree leaves, grass samples, soils, and rainwater, respectively. Insignificant inter-annual variations but significant increase in the ⁷Be activity concentrations during the spring and summer months were observed in birch-tree leaves and grass samples. The seasonal variation of the ⁷Be concentrations in grass samples correlated (R² = 0.4663 and 0.6489) with precipitation. No similar correlation was found for ⁷Be in birch-tree leaves. Beryllium-7 content in birch-tree leaves and in aerial parts of grass was mainly caused by direct transport of ⁷Be from wet deposition into aerial parts of the observed plants.     

Ecological half-life of Cs in mosses and lichens in the Ordu province,Turkey by Cevik and Celik

Twenty-one years after the Chernobyl accident, lichen and moss samples were collected from the Ordu province, which was already chosen for a related study some years ago. It was observed that ¹³⁷Cs activity concentration ranged from 31 to 469 Bq kg⁻¹ in the moss and from 132 to 1508 Bq kg⁻¹ in the lichen samples. The decrease of the activity concentrations in the present measurements (2007) relative to those in 1997 (over a period of 10 y) indicated ecological half-lives between 1.8 and 10.4 y for the moss and between 2.1 and 13.7 y for the lichen samples. It was observed that ¹³⁷Cs was still eminent in the area studied. Moreover, ⁴⁰K activity concentrations and K element concentrations were measured and their relationships were discussed.     

Natural radioactivity in some major rivers of coastal Karnataka on the southwest coast of India

Systematic studies on radiation level and distribution of radionuclides have been carried out in riverine environs of three major rivers of coastal Karnataka, viz. Kali, Sharavathi and Netravathi. The ambient gamma radiation levels along three rivers were measured using a portable plastic scintillometer. Activity concentrations of (226)Ra, (232)Th and (40)K in soil, sediment and rock were measured using a NaI(Tl) gamma-ray spectrometer. In the Kali, Sharavathi and Netravathi riverbanks, the median values of absorbed gamma dose rates in air were found to be 44 nGy h(-1), 35 nGy h(-1) and 57 nGy h(-1), respectively. The highest activity of (226)Ra was found in riverbank soil samples of Sharavathi River. The highest activities of (232)Th and (40)K were found in riverbank soil and sediment samples of Netravathi River. In Kali River, the highest (226)Ra activity was recorded for rock samples. To assess the radiological hazard of natural radioactivity in the samples, absorbed gamma dose rates in air, radium equivalent activity, representative level index, external hazard index and internal hazard index associated with the radionuclides were calculated and compared with internationally recommended values. The representative level index (I(gammar)) values are high in sediment samples of Netravathi River. The radium equivalent activity (Ra(eq)), external hazard index (H(ex)) and internal hazard index (H(in)) values are high in rock samples of Kali River. The results of these investigations are presented and discussed in this paper.