Application of enteric viruses for fecal pollution source tracking in environmental waters

Microbial source tracking (MST) tools are used to identify sources of fecal pollution for accurately assessing public health risk and implementing best management practices (BMPs). This review focuses on the potential of enteric viruses for MST applications. Following host infection, enteric viruses replicate and are excreted in high numbers in the hosts' feces and urine. Due to the specificity in host infection, enteric viruses have been considered one of the most accurate library-independent culture-independent MST tools. In an assessment of molecular viral assays based on sensitivity, specificity and the density of the target virus in fecal-impacted samples, human adenovirus and human polyomavirus were found to be the most promising human-specific viral markers. However, more research is needed to identify promising viral markers for livestock because of cross-reactions that were observed among livestock species or the limited number of samples tested for specificity. Other viral indicators of fecal origin, F+ RNA coliphage and pepper mild mottle virus, have also been proposed as potential targets for developing MST markers. Enhancing the utility of enteric viruses for MST applications through next generation sequencing (NGS) and virus concentration technology is discussed in the latter part of this review. The massive sequence databases generated by shotgun and gene-targeted metagenomics enable more efficient and reliable design of MST assays. Finally, recent studies revealed that alternative virus concentration methodologies may be more cost-effective than standard technologies such as 1MDS; however, improvements in the recovery efficiency and consistency are still needed. Overall, developments in metagenomic information combined with efficient concentration methodologies, as well as high host-specificity, make enteric viruses a promising tool in MST applications.     

Assaying organochlorines in archived serum for a large, long-term cohort: implications of combining assay results from multiple laboratories over time

Conserving irreplaceable, archived serum samples may sometimes conflict with the objective of minimizing measurement error due to laboratory effects. We sought to determine whether we could successfully combine assay results for DDT-related compounds and polychlorinated biphenyls (PCBs) in serum from the same birth cohort obtained from different laboratories over time. Using the Child Health and Development Studies (CHDS) serum archive, we compared variability for assays of a quality control pool to variability for assays of subject serum. The quality control pool was created from native archived serum samples that were pooled, then aliquoted, blinded and inserted pair-wise into assay batches along with the subject serum for 5 studies using CHDS samples conducted over a 13year period by three different laboratories. We found that the variability between laboratory and over time within laboratory was small relative to inter-individual variability for p,p'-DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane), p,p'-DDE (1,1'-dichloro-2,2'-bis(p-chlorophenyl)ethylene) and o,p'-DDT (1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)-ethane). Results were also consistent for most PCB congeners which were detectable in 85% or more of samples. Our results suggest that it is possible to combine assays for DDT and PCB congeners measured at positive levels as they are accumulated for cohort subjects without risking meaningful misclassification due to variation stemming from laboratory or time period. This has significant implications for future study costs, conservation of irreplaceable archived samples and for leveraging past investments for future research. For PCB congeners with very low levels, findings caution against pooling of assays without further exploration.     

A soil sampling intercomparison exercise for the ALMERA network

Soil sampling and analysis for radionuclides after an accidental or routine release is a key factor for the dose calculation to members of the public, and for the establishment of possible countermeasures. The IAEA organized for selected laboratories of the ALMERA (Analytical Laboratories for the Measurement of Environmental Radioactivity) network a Soil Sampling Intercomparison Exercise (IAEA/SIE/01) with the objective of comparing soil sampling procedures used by different laboratories. The ALMERA network is a world-wide network of analytical laboratories located in IAEA member states capable of providing reliable and timely analysis of environmental samples in the event of an accidental or intentional release of radioactivity. Ten ALMERA laboratories were selected to participate in the sampling exercise. The soil sampling intercomparison exercise took place in November 2005 in an agricultural area qualified as a “reference site”, aimed at assessing the uncertainties associated with soil sampling in agricultural, semi-natural, urban and contaminated environments and suitable for performing sampling intercomparison. In this paper, the laboratories sampling performance were evaluated.     

IAEA programme on the quality of marine radioactivity data

Society's growing interest in environmental issues requires the production of reliable information for policy-makers, stakeholders and society in general. This information must be based on accurate data produced by qualified laboratories and data need to be comparable between numerous laboratories for joint assessments, e.g. in International Conventions. The Marine Environment Laboratories of the International Atomic Energy Agency has been providing Quality Assurance services to laboratories involved in marine radioactivity studies for the past 30 years. This has included training, organization of laboratory intercomparison exercises, production of Reference Materials (RMs) and more recently, production of Certified Reference Materials (CRMs) that comply with relevant ISO standards. Here, the overall process of Certification of Reference Materials is reviewed, past work summarized and future needs of marine radioactivity laboratories briefly discussed.     

Air quality management in the WHO European Region--results of a quality assurance and control programme on air quality monitoring (1994-2004)

Since the last decade the WHO Collaborating Centre for Air Quality Management and Air Pollution Control, Berlin, Germany, operates a quality assurance and control (QA/QC) programme on air quality monitoring in the WHO European Region. As main activity Intercomparison workshops have been established for air monitoring network laboratories on a regular basis to harmonise air quality measurements, analysis and calibration techniques. 36 air hygiene laboratories of public health and environmental institutions of 24 countries participated in twelve Intercomparisons between 1994 and 2004. The majority was carried out for NO, NO(2), SO(2) and O(3). The results were predominantly satisfactory for automatic methods. The results of manual methods were mainly in a good, and for several concentration levels partly very good accordance with the data obtained by the monitors.     

Traditional management and conservation of shea trees (<Emphasis Type="Italic">Vitellaria paradoxa</Emphasis> subspecies <Emphasis Type="Italic">nilotica</Emphasis>) in Uganda

Traditional practices are universally recognised as a basis for conservation of biodiversity. However, such practices are often not included in natural resource conservation policies. This study assessed local conservation practices of shea trees (Vitellaria paradoxa) within different farming systems in Uganda and developed conservation guidelines for the species. The assessment involved 300 respondents, 15 focus groups and 41 key informants. Content analysis was used to identify the most important management and conservation practices. Local uses were categorised on the basis of shea tree products while differences in conservation practices were analysed using the Friedman test. The results show that eight shea tree products are used for 36 different purposes. Respondents’ age significantly influenced their knowledge about the shea tree. Traditional conservation practices include on-farm retention during cultivation and the use of folklore (mainly taboos), customs and rituals. Traditional management practices include weeding, bush burning, pollarding and pruning. Based on the current management and traditional conservation practices, a framework for the conservation of shea trees is proposed for integration into conservation policy decisions.     

Air quality management in the WHO European Region — Results of a quality assurance and control programme on air quality monitoring (1994–2004)

Since the last decade the WHO Collaborating Centre for Air Quality Management and Air Pollution Control, Berlin, Germany, operates a quality assurance and control (QA/QC) programme on air quality monitoring in the WHO European Region. As main activity Intercomparison workshops have been established for air monitoring network laboratories on a regular basis to harmonise air quality measurements, analysis and calibration techniques. 36 air hygiene laboratories of public health and environmental institutions of 24 countries participated in twelve Intercomparisons between 1994 and 2004. The majority was carried out for NO, NO₂, SO₂ and O₃. The results were predominantly satisfactory for automatic methods. The results of manual methods were mainly in a good, and for several concentration levels partly very good accordance with the data obtained by the monitors.     

Recent IAEA intercomparison on the determination of trace elements in simulated air filters

Results of a recent intercomparison on the determination of trace elements in simulated air filter samples are reported. A large scatter of results is obtained for some elements and systematic differences between laboratories are observed. The performance of different methods, in particular neutron activation analysis and atomic absorption spectrophotometry, used by the various laboratories is discussed (air filter analysis; intercomparison; trace elements; control charts).     

Blood cadmium,mercury, and lead in children: An international comparison of cities in six European countries,and China,Ecuador, and Morocco

Children's blood-lead concentration (B-Pb) is well studied, but little is known about cadmium (B-Cd) and mercury (B-Hg), in particular for central Europe. Such information is necessary for risk assessment and management. Therefore, we here describe and compare B-Pb, B-Cd and B-Hg in children in six European, and three non-European cities, and identify determinants of these exposures. About 50 school children (7–14 years) from each city were recruited (totally 433) in 2007–2008. Interview and questionnaire data were obtained. A blood sample was analyzed: only two laboratories with strict quality control were used. The European cities showed only minor differences for B-Cd (geometric means 0.11–0.17 μg/L) and B-Pb (14–20 μg/L), but larger for B-Hg (0.12–0.94 μg/L). Corresponding means for the non-European countries were 0.21–0.26, 32–71, and 0.3–3.2 μg/L, respectively. For B-Cd in European samples, traffic intensity close to home was a statistically significant determinant, for B-Hg fish consumption and amalgam fillings, and for B-Pb sex (boys higher). This study shows that European city children's B-Cd and B-Pb vary only little between countries; B-Hg differs considerably, due to varying tooth restoration practices and fish intake. Traffic intensity seemed to be a determinant for B-Cd. The metal concentrations were low from a risk perspective but the chosen non-European cities showed higher concentrations than the cities in Europe.     

Viruses in surface and drinking waters

Over 114 different human enteric viruses are known. They are all excreted into sewage, and opportunities exist for them to find their way into water distribution systems. The characteristics of the illnesses caused by enteric viruses make their transmission by water difficult to recognize. Methods are not available for isolating all of the enteric viruses from water, so that finding any type of human virus in drinking water is an indication that other types may also be present. With improvements in technology, our laboratory has recently been able to detect group B coxsackieviruses, rotaviruses, and hepatitis A virus in chlorinated drinking water. At present, there are no uniform methods for concentrating, isolating, and identifying viruses in water. Emphasis should be placed on seeking uniformity in methods so that standards can be set for viruses in water.     

The Brazilian National Intercomparison Program (PNI/IRD/CNEN): evaluation of 15 years of data

The Instituto de Radioprote??o e Dosimetria has been coordinating the National Intercomparison Program (PNI) for 15 years, from 1991 to 2005. The objective is to evaluate the analytical performance of the laboratories in low-level activity concentration radionuclide assays in environmental samples. This work presents an evaluation of PNI data from 42 intercomparison runs, which distributed to 22 Brazilian laboratories 2511 samples evaluating 5768 radionuclide assays involving 32 radionuclides in the period from 1991 to 2004. The laboratory performance was evaluated using the Normalized Standard Deviation used by the U.S. EPA. For comparison aims, the Normalized Deviation, used by BIPM was also applied. Laboratory performance were grouped and evaluated in three periods of 5 years each. The first period shows, an average value of good performance of 71.2%, the second shows an average of 78.6% and the last an average of 82.3%. Performance for each kind of radionuclide is also presented.     

Radioactivity in environmental samples: Calibration standards measurement methods,quality assurance,and data analysis

The numerous environmental radioactivity measurements made by and for the U.S. Environmental Protection Agency (U.S. EPA) include measurements on samples of water, urine, food, milk, and air filters. Calibration standards are listed which are available in the form of water solutions and soils for a wide range of radionuclides. Method validation procedures for U.S. EPA approval include protocol development and single-laboratory and multiple-laboratory evaluation for precision and accuracy. Interlaboratory comparison studies are conducted for both cross-check and performance evaluation samples and involve 295 federal, state, and local laboratories. For water samples, 80%–90% of the participating laboratories are within the control limits for most of the radionuclides measured; however, some problem areas exist, especially for radium-228 and strontium-89 and -90. For milk and food samples, more than 90% of the laboratories are within control limits for cobalt-60 and cesium-137 but some problems exist for the measurement of strontium-90, iodine-131, and potassium-40. For tritium, 91% of the laboratories are within the control limit for water samples and 87% are within the control limits for the urine samples. The laboratory performance for air filter samples shows some problems for gross beta, strontium-90 and cesium-137 measurements.     

Adaptation of agriculture to climate change in semi-arid Borena,Ethiopia

Livestock production is very risky due to climate variability in semi-arid Sub-Saharan Africa. Using data collected from 400 households in the Borena zone of the Oromia Region, we explored what drives adoption of agricultural practices that can decrease the vulnerability of agro-pastoralists to climate change. Households with more adaptive capacity adopted a larger number of practices. The households’ adaptive capacity was stronger when the quality of local institutions was high. However, adaptive capacity had less explanatory power in explaining adoption of adaptation options than household socio-economic characteristics, suggesting that aggregating information into one indicator of adaptive capacity for site-specific studies may not help to explain the adoption behaviour of households. Strong local institutions lead to changes in key household-level characteristics (like membership to community groups, years lived in a village, access to credit, financial savings and crop income) which positively affect adoption of agricultural practices. In addition, better local institutions were also positively related to adoption of livestock-related adaptation practices. Poor access to a tarmac road was positively related to intensification and diversification of crop production, whereas it was negatively related to the intensification of livestock production, an important activity for generating cash in the region. Our findings suggest that better local institutions lead to changes in household characteristics, which positively affect adoption of adaptation practices, suggesting that policies should aim to strengthen local institutions.     

Effects of a participatory bird census project on knowledge, attitudes and behaviors of coffee farmers in Colombia

This study presents an evaluation of a participatory bird census (PBC) project that has been administered to coffee farmers in Colombia. Our objectives were (1) to evaluate the effect of the PBC project on conservation knowledge, attitudes, and behaviors of coffee farmers and (2) to learn about the barriers farmers perceive toward adopting conservation practices. We conducted 261 interviews on four groups to control for the effects of involvement with an environmental coffee certification program (Rainforest Alliance??RA) and the PBC project. The four groups were (1) non-PBC participant, non-RA certified; (2) PBC participant, non-RA certified; (3) non-PBC participant, RA certified; and (4) PBC participant, RA certified. PBC participant/RA and PBC participant/non-RA were more knowledgeable about migratory and threatened birds. PBC participant/RA, PBC participant/non-RA, and non-PBC participant/RA groups believed they had the skills to perform bird conservation practices on their farms. A majority of respondents indicated that they were performing bird conservation practices and had positive attitudes toward birds. Farmers believed that lack of environmental awareness and lack of knowledge were the main barriers to perform bird conservation practices. Evaluating participatory programs with Colombian farmers can reveal environmental literacy improvements, but self-reported surveys may not be adequate to ascertain attitude changes and adoption of conservation practices. Direct observations on individual farms would be required to determine the impacts on such outreach efforts. Bird conservation seems popular with Colombian coffee farmers, and outreach programs that give detailed biodiversity management information could help aid bird conservation efforts on coffee farms.     

A survey of environmental radioactivity measurements in the United States

A survey of selected radiochemical laboratories in the United States was conducted to estimate the relative numbers and types of analyses performed nationwide for the measurement of environmental radio-activity and to identify the regulatory requirements which affect the selection of analytical procedures and media. Information was sought from each laboratory regarding its parent facility, whether the laboratory performs its own analyses or contracts them out, and the technical and regulatory requirements for which the laboratory performed analyses.Analyses of water, air-particulate, and soil and sediment samples contribute the greatest workload. Many fewer vegetation, milk, diet, gas, or bioassay samples are processed. The most common measurements, especially for water, are for gross-α and gross-β activity. More than 50 different specific-radionuclide analyses are being performed with varying degree of regularity by the 161 laboratories surveyed. γ-spectrometry is the most frequently used method for specific-radionuclide analyses; the use of Ge (Li) detectors for γ-spectrometric measurements now exceeds that of NaI (Tl) detectors. Other radionuclides for which specific analyses are frequently performed by a large percentage (25–75%) of the laboratories include ⁹⁰Sr, ¹³¹I, ²²⁶Ra, ^238,239Pu, ^234,235,238U, and tritium in water (HTO).     

Assessing of Environmental Quality in Six Areas in India,Sri Lanka and Bangladesh

Environmental quality for environmental health has been examined visually by describing general conditions and drinking water supply, sanitation and solid waste treatment conditions and practices in six South-East Asian villages with a dense population. The environmental review was supplemented by discussions with local people and political and administrative decision makers. Some drinking water analyses were done in order to show the water quality to the local people. The quality of the general conditions and the conditions and practices of drinking water supply, excreta disposal and solid waste treatment practices have been graded with the grading system presented below. The grading could be done in 1 or 2 days in each area by two persons, of which one was an environmental scientist and the other a social scientist, who also knew the local culture and worked as a translator.     

How do soil and water conservation practices influence climate change impacts on potato production? Evidence from eastern Canada

We used a stochastic production function method together with a farm-level dataset covering 18 farms over a 23-year period to assess the role that soil and water conservation practices play in affecting the climate change impacts on potato yield in northwestern New Brunswick, Canada. Our analysis accounted for the yield effects of farm inputs, farm technologies, farm-specific factors, seasonal climatic variables, soil and water conservation practices, and a series of interaction terms between soil and water conservation practices and climatic variables. Regression results were used in combination with three climate change scenarios developed by the Intergovernmental Panel on Climate Change (A2, A1B, B1) and four general circulation model predictions over three 30-year time periods (2011–2040, 2041–2070, and 2071–2100) to estimate a range of potato yield projections over these time periods. Results show that accounting for soil and water conservation practices in climate–yield relationships increased the impacts of climate change on potato yield, with yield increases of up to 38 % by the 2071–2100 period. These findings provide evidence that adoption of soil and water conservation practices can help boost potato production in a changing Canadian climate.     

The effect of diesel exhaust on sperm-shape abnormalities in mice

The sperm-shape abnormality bioassay in mice was used to determine whether chemical mutagens in diesel exhaust reach the testes. Strain A male mice (30 per group from 4 to 6 weeks of age) were exposed for 31 or 39 weeks to either diesel exhaust or clean air. After exposure, Eosin Y-stained, air-dried smears of cauda epididymal sperm were scored for changes in sperm-head abnormalities in three different laboratories. There was no difference in the proportion of abnormally shaped sperm in controls and mice exposed to diesel exhaust.     

Contour Farming Based on Natural Vegetative Strips: Expanding the Scope for Increased Food Crop Production on Sloping Lands in Asia

In the agriculture of the future, there is a compelling place for agroecologically-based practices alongside practices based on the best available chemical, genetic, and engineering components. This paper explores this issue in the context of the development and spread of a conservation farming system based on natural vegetative contour buffer strips in smallholder production systems in southeast Asia. Farmers adapted contour hedgerow farming practices into a simpler, buffer-strip system as a labor-saving measure to conserve soil and sustain yields on steeply sloping cropland in Claveria, Mindanao, Philippines. Permanent-ridge tillage systems were also adapted to smallholder farming systems by researchers. Natural vegetative buffer strips resulted in gradually increasing yields, with an estimated benefit of 0.5t/ha/crop. They were seen to increase land values, facilitate investment in more intensive and profitable cropping systems, and expand the land base for food crop agriculture. They induced an institutional innovation of farmer-led Landcare organizations, which have spread this and other agroforestry practices to thousands of households in the southern Philippines.     

Measurements of 60Co in spoons activated by neutrons during the JCO criticality accident at Tokai-mura in 1999

Neutron activated items from the vicinity of the place where the JCO criticality accident occurred have been used to determine the fluence of neutrons around the facility and in nearby residential areas. By using underground laboratories for measuring the activation products, it is possible to extend the study to also cover radionuclides with very low activities from long-lived radionuclides. The present study describes gamma-ray spectrometry measurements undertaken in a range of underground laboratories for the purpose of measuring (60)Co more than 2 years after the criticality event. The measurements show that neutron fluence determined from (60)Co activity is in agreement with previous measurements using the short-lived radionuclides (51)Cr and (59)Fe. Limits on contamination of the samples with (60)Co are evaluated and shown to not greatly affect the utility of neutron fluence determinations using (60)Co activation.