Structure and Differentiation of Spruce Populations in Eastern Europe and Western Siberia

The structures and differentiation patterns of the Norway spruce (Picea abies) and Siberian spruce (P. obovata) populations have been studied in a wide area, including the transition zone of their continuous geographic range. The areas occupied by transitional populations have been detected. Series of distinct populations of the Norway and Siberian spruces are located to the west and east of them, respectively.     

Structure and differentiation of spruce populations in the Komi Republic

Groups of spruce populations growing in western and southwestern, central, and northern regions of the Komi Republic significantly differ from each other. Trees with a phenotype intermediate between Norway (Picea abies) and Siberian (P. obovata) spruce prevail in the first group, their proportion in the second group does not exceed 20%, and almost all trees in the third group have the Siberian spruce phenotype.     

Appropriateness of needles of different conifer species for the feeding and growth of larvae from two populations of the Siberian moth

A comparison of two Siberian moth populations from the Altai Republic (feeding on Siberian fir) and the Tyva Republic (feeding on Siberian larch) has revealed no differences between them with respect to ecophysiological parameters of larval growth and feeding on needles of the same conifer species under laboratory conditions. The index of appropriateness of food plants for the larvae from both populations decreases in the series larch > fir > spruce > pine. The age-related dynamics of parameters characterizing the feeding and growth of larvae are independent of the population to which the insects belong.     

Distribution of frost injuries in the wood of conifers

The distribution of frost injuries in earlywood and latewood, in different zones of stem (with respect to height, diameter, and circumference), and at the bases of large roots has been studied in Siberian spruce (Picea obovata Ledeb.) and Siberian larch (Larix sibirica Ledeb.) at the northern limit of their ranges (the Obsko-Tazovskaya forest-tundra). It has been shown that a high frequency of frost injuries, especially in spruce, is characteristic of the study region. Mass frost injuries occur only in thin trees, mainly in the lower part of stems. Guidelines for collecting and preparing wood samples for the study of frost injuries are proposed.     

The hypothesis of hydrochorous dissemination of populations of conifers

A comparative analysis of field observations and experiments on the distance and rate of dispersal of self-sown Scotch pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) progeny has shown that the rate of their hydrochorous dissemination downstream is an order of magnitude higher than the rate of dispersal in upland areas. A hypothesis assigning priority to the role of hydrochory in the expansion of conifer populations to the tundra (periglacial) zone along with climate warming has been formulated and substantiated by comparative isozyme analysis of populations in transects lying along and across rivers.     

Morphophysiological responses of Pinus sylvestris L. and Picea obovata Ledeb. to industrial pollution under conditions of Northwestern Russia

Morphophysiological parameters of needles have been studied in Scots pine (Pinus sylvestris L.) and Siberian spruce (Picea obovata Ledeb.) trees of different life states growing under conditions of industrial air pollution with sulfur and heavy metals. Three functional clusters (tentatively named the initial, transitional, and disastrous clusters), each characterized by a certain metabolic status, have been revealed, and their quantitative and qualitative structure has been evaluated. The morphophysiological responses of pine and spruce are shown to be qualitatively different, depending on differentiation of trees with respect to vitality under conditions of industrial pollution. Probable factors accounting for these differences are discussed.     

Mitochondrial DNA variation pattern and postglacial history of the Siberian larch (<Emphasis Type="Italic">Larix sibirica</Emphasis> Ledeb.)

Mitochondrial DNA (mtDNA) variation has been studied in populations of the Siberian larch with the use of five PCR-based markers characterizing 29 haplotypes. The studied populations from the main parts of the Siberian larch range belong to several geographically isolated groups with their specific sets of haplotypes, which have probably originated from separate refugia for forest vegetation of the last glacial maximum. Conclusions concerning the possible location of these refugia and the ways and pattern of the postglacial recolonization of the recent Siberian larch range are drawn. The spatial distribution of haplotypes within individual populations is nonrandom. The typical size of the area occupied by a group of plants with the same haplotype is 50–100 m across.     

Response of the pigment system of conifers to long-term industrial air pollution

To estimate the response of conifers to long-term industrial air pollution at the pigment system level, the dynamics of photosynthetic pigments have been studied in needles of Scots pine (Pinus sylvestris L.) and Siberian spruce (Picea obovata Ledeb.) growing at different distances from the emission source (a pulp and paper mill). It has been shown that the pigment apparatus of spruce. The results show that in spruce, unlike in pine, the pigment apparatus remains unchanged under the effect of weak pollution. Long-term exposure at high levels of industrial emission results in suppression of chlorophyll and carotenoid syntheses in pine but stimulates pigment production in spruce. An increase in the contents of pigments in the needles of conifers growing in the impact zone of the pulp and paper mill reflects a compensatory mechanism of adaptation of their pigment system to long-term stress exposure.     

Reference populations for discriminant analysis in the continuous range of Norway and Siberian spruces

Reference populations identified within the continuous range of Norway (Picea abies) and Siberian (P. obovata) spuces significantly differ in biometric characters of seed scales, relative position in the same coordinate system (Root 1 and Root 2), phenotypic structure, and mutual taxonomic position (according to the squared Mahalanobis distance). Due to the symmetrically equal positions of the populations relative to each other, they can be used as reference populations in discriminant analysis at a two-, three- or five-class gradation of the test character.     

Genetic divergence of eastern European and Tobol populations of Calluna vulgaris (L.) Hull.

Analysis of geographic variation of chloroplast DNA in the eastern European and western Siberian (Tobol) groups of Calluna vulgaris populations isolated from each other for a long period of time has revealed distinct differentiation between them. It has been shown that three populations growing in the Russian Plain and Karelia share two haplotypes characteristic of central Europe, while three Tobol populations contain one haplotype F occurring in Sweden. These results confirm the hypothesis of genetic divergence between the Tobol and eastern European groups of C. vulgaris populations.     

Mineral composition of assimilative organs of conifers after reduction of atmospheric pollution in the Kola Peninsula

An analysis has been made of changes in the mineral composition of conifer needles in northern taiga forests after reduction of emissions from the Severonickel Copper-Nickel Smelter Complex in the city of Monchegorsk, the most powerful source of air pollution in Northern Europe. The data obtained in permanent monitoring plots in 1991, 1993, 2000, and 2007 have been compared. The results provide evidence for reduction in the concentrations of heavy metals (Cu, Ni, Fe) and sulfur in the needles of spruce and pine trees in areas with different levels of air pollution. However, mineral nutrition of conifers has not been optimized. The plants are insufficiently supplied with nutrient elements such as P, Mg, Mn, and Zn. The contents of these and other elements essential for the normal functioning of needles (in particular, Ca, K, and P) continue to decrease, especially in pollution-induced sparse spruce forests. The most significant alterations of nutrient regime have been revealed in the Siberian spruce.     

Ecological factors influencing frequency of Norway spruce butt rot in mature stands in Lithuania

We assess the influence of edaphic, climatic, stand structure and individual tree (stump diameter) factors on incidence of Norway spruce (Picea abies (L.) H. Karst.) butt rot. The data used in the study come from 97 stands, originated from natural regeneration, which were clear-cut. The age of the stands varied between 71 and 130 years old. The climate continentality, site fertility, hydrotop (site humidity index), part of Norway spruce in the stand, stand stocking level (density index) and stump diameter were selected as the factors related with frequency of decay. The study material revealed that selected variables explain 34% of Norway spruce butt rot incidence in Lithuanian forests. Site fertility and stand age influence on butt rot incidence was weak. Higher incidence was related with low stand density, low site humidity (dry sites) and higher part of Norway spruce in stand. More frequent damages were recorded on higher diameter stumps. Our results showed longitudinal distribution of decay incidence in Lithuania—the frequency of decay increasing from West (coast) to East (continent).     

Effects of environmental factors on the seasonal growth of <Emphasis Type="Italic">Picea abies</Emphasis> L. (Karst.) in northern Karelia

The seasonal growth of shoots, needles, and the trunk in the Norway spruce has been studied in a bilberry spruce forest in the middle taiga subzone of Karelia. The results show that the growth of vegetative organs in this tree species depends mainly on the air temperature regime, whereas the effect of variations in factors such as the period of sunshine, precipitation, and air humidity is relatively weak.     

Decline of spruce (<Emphasis Type="Italic">Picea abies</Emphasis>) in forests of Belarus

The decline of spruce stands in Belarus has been analyzed in relation to the dynamics of climatic variables. The results show that this process is correlated with the amount of precipitation, moisture deficit, index of aridity, relative air humidity, and evapotranspiration. Frosts at the onset of the growing season enhance tree die-off, while increase in cloud cover has a favorable effect on the state of spruce stands. Damage to trees occurs mainly in areas with elevated and convex topography and slopes of southwestern aspect, increasing on steeper slopes. The level of die-off is most closely correlated with conditions of the previous year, which is explained by the impact of biological factors (pest insects and phytopathogens) on tree stands already affected by water stress. The decline of spruce stands on a mass scale is also observed in neighboring regions of Russia and counties of East Europe, which is evidence for a low adaptability of spruce to current climate change, including the increasing frequency and severity of dry periods.     

Formation of Two Xylem Frost Injuries in One Annual Ring in Siberian Spruce under Conditions of Western Siberian Forest-Tundra

The phenomenon of the formation of two damaged xylem layers in one frost ring is described in Siberian spruce grown in western Siberian forest-tundra. Temperature conditions providing for the formation of pathological cell and tissue structures are determined. The relationship between the formation of double frost injuries and cold periods is demonstrated.     

Reproduction of the Siberian spruce in the timberline ecotone of the Iremel’ Massif

Quantitative and qualitative characteristics of cones and seeds and the emergence and survival of seedlings in the Siberian spruce (Picea obovata Ledeb.) were studied in different parts of the timberline ecotone on the Iremel’ Massif, the Southern Urals, from 2003 to 2006. In the productive year 2003, a decrease in the number of cones (from 110000 to 3000 per hectare), the yield of seeds (from 13 × 10⁶ to 0.09 × 10⁶ per hectare), and their laboratory germination rate (from 34 to 14%) with an increase in elevation above sea level was recorded. In 2004, significant differences in the number of seedlings per hectare were revealed between the lower, middle, and upper parts of the ecotone (85 200, 19 800, and 0–400 ind., respectively). It has been shown that the greater part of seedlings (76–96%) perish during the first cold period. New generations of spruce trees are formed only after productive years. Seed production and seedling mortality in spruce apparently depend not only on air temperature and soil moistening but also on snow depth and soil temperature, which change more abruptly along the altitudinal gradient.     

Seasonal changes in the structure of <Emphasis Type="Italic">Picea obovata</Emphasis> mycorrhizas in the middle taiga subzone

Seasonal changes in the occurrence frequency of fungal coats and in dimensional anatomical characters of ectomycorrhizal endings in the roots of Siberian spruce have been studied in two middle taiga phytocenoses. Twelve subtypes of fungal coats have been identified, with their occurrence frequency changing during the growing season. Seasonal fluctuations have been revealed in the dimensional anatomical characters of ectomycorrhizae, with the average thickness and volume proportion of fungal coats slightly increasing by the end of the growing season.     

Population structure in European and Siberian spruces according to their phenotypes

Structure and diversity of populations in the European and Siberian spruces were studied by their phenotypes. On vast areas of their total range in the east of Europe and Siberia, significant variability of intrapopulation diversity index in the frequency of individual phenotypes is observed. All analyzed populations are divided into two large groups: with low (and decreased) and high (and increased) levels of intrapopulation diversity. A high level of diversity was observed in the regions with hybrid or intermediate populations, while a low level was in the regions with populations of European and Siberian spruces without genetic interference. Mean level of diversity is typical for the areas with populations transient in these characters.     

Analysis of meiotic chromosome aberrations in Siberian spruce (Picea obovata Ledeb.) under conditions of natural and technogenic stress

Meiotic abnormalities have been studied in Siberian spruce growing in the Southern Urals under different ecological conditions: in contrasting natural landscapes (high mountains, plains, river valleys and floodplains), in zones of natural geochemical anomalies, and in areas with different levels of industrial pollution. The results provide evidence for an adverse effect of technogenic pollution and extreme environmental factors on the process of microsporogenesis in the species studied.     

The Holocene dynamics of vegetation and climatic conditions on the eastern slope of the Subpolar Urals

Changes in the vegetation and climatic conditions on the eastern slope of the Subpolar Urals over the past 10000 years have been reconstructed on the basis of integrated palynological, botanical, and radiocarbon analysis of material from two sections of peat deposits in the floodplains of the Lyapin and Man’ya rivers (the Severnaya Sos’va basin). The dynamics of regional vegetation have been traced: from the herb–shrub tundra in the late postglacial time to the spruce–larch forest–tundra and sparse larch–birch–spruce stands in the Early Holocene, to birch–pine–spruce forests with an admixture of fir in the Middle Holocene, and to northern taiga forests with dominance of Scots pine and Siberian stone pine (similar to present-day forests) in the Late Holocene. The results show that the northern taiga zone of the study region in the period between approximately 5500 and 2500 years BP was occupied by forests of middle and southern taiga facies, as the climate was significantly warmer than it is today.