Reevaluation of the sex pheromone of the lesser date moth, Batrachedra amydraula, using autosampling SPME-GC/MS and field bioassays

In a previous study of the sex pheromone of the lesser date moth, Batrachedra amydraula, using laboratory females, (Z4,Z7)-4,7-decadien-1-yl acetate, Z4-decen-1-yl acetate, Z5-decen-1-yl acetate and decyl acetate were identified by sequential SPME-GC/MS analysis. Traces of Z5-decen-1-ol were detected only in airborne collections. Concomitant field tests and re-evaluation of the composition of the sex pheromone of B. amydraula with feral female moths, using an improved sequential SPME-GC/MS procedure revealed a complex mixture of candidate pheromonal compounds. The same unsaturated acetates and the corresponding alcohols: (Z4,Z7)-4,7-decadien-1-ol, Z4-decen-1-ol, Z5-decen-1-ol were positively identified. In addition, the corresponding aldehydes, octanol and octyl acetate were also detected. All compounds were found to be released in a circadian rhythm, in a narrow time window of 2 h, approximately 1 h before sunrise. Comprehensive field bioassays indicated that the optimal attractive blend is a three-component mixture of (Z4,Z7)-4,7-decadien-1-yl acetate, Z5-decen-1-yl acetate and Z5-decen-1-ol in a ratio of 1:2:2. This blend gave about fivefold higher trap catch of B. amydraula males as compared to the previously published binary blend of (Z4,Z7)-4,7-decadien-1-yl acetate and Z5-decen-1-yl acetate in a ratio of 1:2. The alcohol Z5-decen-1-ol is an essential synergistic component of the sex pheromone of B. amydraula. All other identified compounds are inert, being neither synergists nor inhibitors of the pheromone. The optimal pheromone blend will be a useful tool in monitoring and control of B. amydraula, which is a serious pest of date plantations throughout the Middle East and northern Africa.     

Computer simulation applied to the biological control of the insect Aphis gossypii for the parasitoid Lysiphlebus testaceipes

In integrated pest management (IPM), biological control is one of the possible options for the prevention or remediation of an unacceptable pest activity or damage. The success of forecast models in IPM depends, among other factors, on the knowledge of temperature effect over pests and its natural enemies. In this work, we simulated the effects of parasitism of Lysiphlebus testaceipes (Cresson, 1880) (Hymenoptera: Aphidiidae) on Aphis gossypii (Glover, 1877) (Hemiptera: Aphididae), a pest that is associated to crops of great economic importance in several parts of the world. We made use of experimental data relative to the host and its parasitoid at different temperatures. Age structure was incorporated into the dynamics through the Penna model. The results obtained showed that simulation, as a forecast model, can be a useful tool for biological control programs.     

Identification of the sex pheromone of Conogethes pluto: a pest of Alpinia

In recent years, Conogethes pluto (Lepidoptera: Crambidae) has become a major pest of Alpinia and other ornamental gingers in the Northern Territory and Queensland, Australia. This pest damages the flowers and bores into the stems, causing substantial losses to production. Currently, no synthetic sex pheromone is available to monitor or control this pest. This work aims at the identification of the sex pheromone of this pest. Analysis of the sex pheromone gland of female C. pluto by gas chromatography/electroantennogram detector revealed the presence of seven candidate pheromone compounds that elicited electroantennogram responses. Using gas chromatography/mass spectrometry analysis and micro-derivatization reactions, six compounds were identified as (E)-10-hexadecenal, as the main pheromone compound, (Z)-10-hexadecenal, hexadecanal, (E)-10-hexadecen-1-ol, (10E,12E)-hexadeca-10,12-dienal and (3Z,6Z,9Z)-tricosa-3,6,9-triene as minor pheromone compounds. In two-field trapping experiments, C. pluto responded to the six-component blend, and three of six compounds, i.e., (E)-10-hexadecenal, (3Z,6Z,9Z)-tricosa-3,6,9-triene, and (10E,12E)-hexadeca-10,12-dienal were shown to be necessary for attraction. In a subsequent experiment testing various doses (i.e., 0.01, 0.1, and 1 mg) of the six-component blend, the largest number of males was captured in traps baited with a lure loading of 1 mg. The availability of the sex pheromone of C. pluto will enable monitoring and provides the basis for additional control options for this pest.     

Behavioral ecology of the neotropical termite-hunting ant Pachycondyla (= Termitopone) marginata: colony founding,group-raiding and migratory patterns

This study provides the first detailed field account of colony founding, group-raiding and migratory habits in the neotropical termite-hunting ant rPachycondyla marginata, in a semi-deciduous forest in south-east Brazil. New colonies can originate by haplometrosis, pleometrosis, or colony fission. Incipient colonies with multiple foundresses persisted longer in the field, and most excavated nests contained more than one dealated female. A total of 202 group raids by P. marginata were registered, and in all cases the raided termite species was Neocapritermes opacus. Nearly 20% of the workers within a colony engage with raiding activity. Colonies of P. marginata hunt for termites approximately every 2–3 weeks, and group-raids may last for more than 24 h. Target termite nests are up to 38 m from the ant colony, and occasionally two nests are simultaneously raided by one ant colony. Raiding ants carry 1 or 2 paralysed prey, and nearly 1600 termites can be captured during a 9-h raid. Migration by P. marginata colonies lasted over 2 days and covered distances of 2-97 m (n = 48). Average residence time at a given location was 150 days. Three basic migratory patterns were noted: colony fission (only part of the colony moves), long-distance migrations, and short-distance migrations. Both raiding and migratory activities appeared to be strongly affected by seasonal factors. The group raiding and migratory patterns of P. marginata are compared with other ant taxa with similar habits. It is concluded that P. marginata presents a rudimentary form of the so-called army ant behavior, which is highly developed in the subfamilies Dorylinae and Ecitoninae. The extremely specialized diet of P. marginata and the associated high costs of migration are features likely to prevent it from evolving a full army ant life pattern.     

Hierarchical Bayesian Modelling of plant colonisation by winged aphids: Inferring dispersal processes by linking aerial and field count data

Understanding and modelling insect pest dispersal is an important prerequisite for designing integrated pest management programs. Nevertheless, studies investigating the dispersal of small insects in natural conditions remain scarce mainly because of the difficulty of tracking the movements of these organisms. Here we propose to use Hierarchical Bayesian Modelling (HBM) framework to gain knowledge on hidden processes that cannot be observed directly in natura, such as insect landing and insect mortality, through the definition of latent variables. An HBM describing crop colonization by winged aphids was fitted to a large dataset of field observations issued from a long term survey at a wide scale of both aerial and field densities of the bird cherry-oat aphid Rhopalosiphum padi. This study provides the first evidence that suction trap data are reliable proxies of aphid colonizing rates in cereal fields in autumn and can be a nice alternative to the very time-consuming crop sampling. The proportion of winged aphids landing in cereal fields is shown to vary between regions according to the degree of investment of local R. padi population in sexual reproduction. Results also indicate that under autumnal field conditions, less than 5% of winged aphids survive more than 10 days after landing. This HBM provides the basis of a predictive model for aphid crop colonization that fully accounts for all sources of uncertainty. It should be of great value to improve the trust of users in any decision making systems.     

Incorporating natural enemies in an economic threshold for dynamically optimal pest management

The control of pests by their natural enemies represents an important regulating ecosystem service that helps maintain the stability of crop ecosystems. These services, however, are often ignored in pest management decision making. In addition, the use of broad-spectrum insecticides can damage the populations of natural enemies, reducing the cost-effectiveness of insecticide investment if unaccounted for in treatment decisions.The existing literature on modeling of biological control of insect pests has generally focused on simulations of the population dynamics of pest and natural enemy species and the processes underlying pest control. But agriculture is a managed ecosystem where predator–prey relationships are heavily influenced by human managers. In modeling managerial choices, this study develops an intra-seasonal dynamic bioeconomic optimization model for insecticide-based pest management that explicitly takes into account both the biological control effect of natural enemies on pest density and the nontarget mortality effect of insecticides on the level of natural pest control supplied. The model captures predator–prey interactions, linking them to crop growth and yield damage functions, which in turn are evaluated in a dynamic optimization framework. We introduce a new decision rule for judicious insecticide decisions using a natural enemy-adjusted economic threshold. This threshold represents the pest population density at which insecticide control becomes optimal in spite of the opportunity cost of injury to natural enemies of the target pest. Using field data from Michigan, the model is applied to the case of soybean aphid (Aphis glycines, Matsumura), a recent invasive pest of soybean (Glycine max), whose management is of both economic and environmental importance to the North Central region of the United States. As illustrated by the numerical examples, such natural enemy-adjusted threshold is likely to lead to fewer recommendations for insecticide use than naïve models that ignore natural enemies, resulting in less insecticide use, while maintaining profitability for farmers that rely on chemical pest control methods.The bioeconomic model developed in this study can be used to conduct a wide variety of analyses such as identifying dynamically optimal spray strategies and estimating the implied economic value of natural control services. Furthermore, with the incorporation of inter-year carry-over factors, such as overwintering of pests and natural enemies, the current model can contribute to building multi-year models for studying long-term pest management.     

Fragmentation of Phragmites Habitats, Minimum Viable Population Size, Habitat Suitability, and Local Extinction of Moths, Midges, Flies, Aphids, and Birds

Results from populations of insects and birds inhabiting Phragmites habitats were used to analyze effects of fragmentation. Flush-crash cycles of the stem-boring moth Archanara geminipuncta (Lepidoptera, Noctuidae) showed regionally concurrent, local extinctions despite an originally enormous population size (more than 180,000 adults), emphasizing the importance of metapopulation dynamics. Further, A. geminipuncta could be considered a keystone species, since shoot damage facilitated more than twenty species of herbivores, saprovores (of the caterpillars' feces), and their parasitoids. The gall midge Lasioptera arundinis could survive only in side shoots induced by shoot damage of A. geminipuncta .
Small Phragmites stands had thinner shoots (due to a water or nutrient deficiency) and shoots with more leaves (due to a better light supply) than large stands, thereby influencing species-specific demands for habitat suitability and nutritiousness of reed tissue. In other words significance of habitat fragmentation could not be assessed by area alone. For example, two chloropid flies depending on thin, yellowish shoots survived only in small habitats or in the unmown edges of large habitats.
Local persistence of Phragmites herbivores depended on much larger population sizes than could be expected from a population size sufficient to maintain genetic variation. At least 11,000 adults of the gall midge Giraudiella inclusa (or more than 84,000 galls) were necessary to avoid local extinction.
With regard to conservation management of reed habitats, nature reserves should consist of old and unmown reeds, have fewer disturbed (particularly, fewer mown) habitat edges, measure more than two hectares (priority should go to the largest remaining fragments), and be surrounded by nearby reed habitats providing reservoir populations and diverse shoot types.     

Host-plant genotypic diversity mediates the distribution of an ecosystem engineer

Ecosystem engineers affect ecological communities by physically modifying the environment. Understanding the factors determining the distribution of engineers offers a powerful predictive tool for community ecology. In this study, we examine whether the goldenrod bunch gall midge (Rhopalomyia solidaginis) functions as an ecosystem engineer in an old-field ecosystem by altering the composition of arthropod species associated with a dominant host plant, Solidago altissima. We also examine the suite of factors that could affect the distribution and abundance of this ecosystem engineer. The presence of bunch galls increased species richness and altered the structure of associated arthropod communities. The best predictors of gall abundance were host-plant genotype and plot-level genotypic diversity. We found positive, nonadditive effects of genotypic diversity on gall abundance. Our results indicate that incorporating a genetic component in studies of ecosystem engineers can help predict their distribution and abundance, and ultimately their effects on biodiversity.     

Using Citizen Science Programs to Identify Host Resistance in Pest‐Invaded Forests

Abstract: Threats to native forests from non‐native insects and pathogens (pests) are generally addressed with methods such as quarantine, eradication, biological control, and development of resistant stock through hybridization and breeding. In conjunction with such efforts, it may be useful to have citizen scientists locate rare surviving trees that may be naturally pest resistant or tolerant. The degree of resistance of individual trees identified in this way can be tested under controlled conditions, and the most resistant individuals can be integrated into plant breeding programs aimed at developing pest‐resistant native stock. Involving citizen scientists in programs aimed at identifying rare trees that survive colonization by pests provides a low‐cost means of maximizing search efforts across wide geographic regions and may provide an effective supplement to existing management approaches.     

Variability over space and time in the genetic structure of the winged kelp Alaria marginata

The genetic structure of Alaria marginata Postels & Ruprecht was investigated spatially and seasonally using amplified-fragment length polymorphisms (AFLP). Using one primer, 206 scoreable bands were produced. Individual plants that were separated by as little as a few decimeters to >100 km could be distinguished, and followed an isolation-by-distance model. Genetic similarity (average April data) ranged from 76% for patches (a few decimeters in diameter), to 71% for individual kelp stands (15 m across) and 67% for a group of stands separated by 185 km. Greater genetic similarity of patches occurred at a wave-sheltered site than at a wave-exposed site. The lower wave action may limit meiospore dispersal, resulting in patches of related sporophytes. Genetic similarities between A. marginata stands indicated a pattern of gene flow that is consistent with local currents. In one stand, genetic similarities were markedly different between seasons. This seasonal pattern may be the result of different age groups dominating the sampled stands over time. The genetic structure of A. marginata comprises local scale (patch and within-stand) heterogeneity and larger scale (between-stands) homogeneity. Received: 30 September 1998 / Accepted: 26 October 1999     

Bioremediation of toxic metals mercury and cesium using three types of biosorbent: bacterial exopolymer,gall nut,and oak fruit particles

Cesium and mercury are two mono-valent elements which can be found in toxic industrial, medical, and nuclear wastes. Their presence in the environment has deleterious effects on the ecosystem, living organisms including humans. Due to the chemical nature of these metals, bioremediation by conventional methods is more difficult to achieve compared to other metals. In this study, we used three biosorbents (oak powder, gall nut, and bacterial exopolymer) for the bioremediation of Hg and Cs. Bio-polymer was produced in the GMS mineral broth. Synthetic wastes of Hg(NO₃)₂ and isotope Cs-133 as the single-metal solutions were used. The biorefining process was carried out in glass columns, made of Pyrex, with dimensions 20?×?7/2?cm² with a V-shaped bottom. The samples were analyzed using atomic absorption. The experimental results showed that eliminated metal percent by oak powder, gall nut, and bacterial exopolymer were, respectively, of 94.8%, 96%, and 13.8% for Hg and 7.8%, 4.4%, and 69.4% for Cs. The tests revealed that Ca⁺⁺, when used as flocculant, played a key role in both biosorption and bio-precipitation rates. Consequently, it was concluded that the investigated biosorbents could be use as an integrated biosorption system for the refinement of mixed wastes.     

Condition-dependent sex allocation by clones of a galling aphid

Local mate competition (LMC) has been postulated to be the primary factor of female-biased sex allocation. In animals such as aphids that exhibit seasonal alternations of clonal and sexual reproduction, there is a high possibility of intra-clonal mating and LMC. This possibility is more plausible for more fecund clones, but outbreeding is predicted for less fecund clones. We hypothesize that clones that are more fecund will gain higher fitness returns by reducing investment in males because of more intense LMC among clonal males. We tested this hypothesis by elucidating the clonal sex allocation patterns of the galling aphid Kaltenbachiella japonica, in which inbreeding and LMC appear to be common. Winged mothers that emerge from a gall, belonging to the same clone, produced males and sexual females asexually on a branch, without dispersing to other trees. The heavier the gall, the more winged mothers were produced from the gall. Individual mothers produced a constant number of males and a variable number of females. The clonal sex allocation to males was 39.8?%, on average, and decreased with increasing gall weight. This result showed that clones that were more fecund exhibited more female-biased sex allocation and thus supported our hypothesis. Furthermore, our results corroborated Stubblefield and Seger’s hypothesis for sex allocation in patch structure rather than Yamaguchi’s constant male hypothesis. We conclude that K. japonica clones are able to adjust their sex allocation patterns adaptively depending on the quality of resources in the galls.     

Bewertung von diffusen Stoffeinträgen im Rahmen der nationalen Biodiversitätsstrategie

Background, aim and scope In 2007, the German government approved a national strategy on biological diversity. The strategy requires the integration of its quality targets and action targets into other policies. In addition to other pressure factors, area-wide diffuse substance discharges contribute considerably to the loss of biological diversity. Reducing these diffuse substance discharges to long-term ecologically compatible levels is one of the main action targets. This paper aims to provide an overview of preconditions for implementing this objective in terms of content as well as organization and to point out important deficits. Main focus To achieve this, the paper will discuss environmental monitoring and its implementation in Germany drawing on the targets of the national strategy on biological diversity. It will discuss the opportunities for the evaluation of the German and European biodiversity strategies using monitoring required in the execution of environmental laws and regulations as well as international substance-related indicators. Results Environmental monitoring in Germany and in Europe is characterized by a plurality of monitoring networks operated under different administrative competences and environmental sectors. Only a small part of the collected data is evaluated in a cross-media and cross-sectoral perspective even though the linking of the different data sets is technically possible. Discussion A key problem is that harmonized minimum requirements to monitor the reduction of diffuse substance discharges are missing. Without the implementation of a cooperative and harmonized monitoring approach and organizational guidelines, the reduction of diffuse substance discharges will not be verifiable. Environmental data as well as indicators are essential to evaluate the success of the national biodiversity strategy. Suitable monitoring concepts are available in the German Federal Republic and its Federal States, nevertheless, a political impulse for their implementation is missing. Recommendations and perspectives The national biodiversity strategy may reopen the discussion on the necessity of an integrated monitoring. A coordinated approach that refers both to the international indicator-discussion and to institutional obstacles and aims to develop a proposal for integrated monitoring data is necessary.     

Echolocation beam shape in emballonurid bats, Saccopteryx bilineata and Cormura brevirostris

The shape of the sonar beam plays a crucial role in how echolocating bats perceive their surroundings. Signal design may thus be adapted to optimize beam shape to a given context. Studies suggest that this is indeed true for vespertilionid bats, but little is known from the remaining 16 families of echolocating bats. We investigated the echolocation beam shape of two species of emballonurid bats, Cormura brevirostris and Saccopteryx bilineata, while they navigated a large outdoor flight cage on Barro Colorado Island, Panama. C. brevirostris emitted more directional signals than did S. bilineata. The difference in directionality was due to a markedly different energy distribution in the calls. C. brevirostris emitted two call types, a multiharmonic shallowly frequency-modulated call and a multiharmonic sweep, both with most energy in the fifth harmonic around 68?kHz. S. bilineata emitted only one call type, multiharmonic shallowly frequency-modulated calls with most energy in the second harmonic (~46?kHz). When comparing same harmonic number, the directionality of the calls of the two bat species was nearly identical. However, the difference in energy distribution in the calls made the signals emitted by C. brevirostris more directional overall than those emitted by S. bilineata. We hypothesize that the upward shift in frequency exhibited by C. brevirostris serves to increase directionality, in order to generate a less cluttered auditory scene. The study indicates that emballonurid bats are forced to adjust their relative harmonic energy instead of adjusting the fundamental frequency, as the vespertilionids do, presumably due to a less flexible sound production.     

Quantitative and qualitative observations on Laminaria dentigera and other subtidal kelps of southern Kodiak Island,Alaska

Observations were made and quantitative samples of non-canopy kelps were collected, using SCUBA, at 9 subtidal sites off southern Kodiak Island in May, 1976. At a 10th site, only observations were made. The most abundant species in the quadrats were Laminaria dentigera, L. yezoensis, Pleurophycus gardneri, Agarum cribrosum, and Alaria marginata. We found fertile plants of all these species. L. dentigera dominated at all sampled sites except one within a bed of Nereocystis luetkeana, where P. gardneri was dominant. L. yezoensis was dominant at the site which was visited but not sampled. The average wet weight of non-canopy kelps in the 55 samples was 12 kg m^-2, and the quadrat range was 2 to 35 kg m^-2. The reported ranges of P. gardneri and Alaria marginata are extended westward from Montague Island (147°22W) to Bumble Bay, Kodiak Island (154°43W), where A. marginata was found at a depth of 8.5 m.     

Sulfuric acid in the phaeophyte alga Desmarestia munda deters feeding by the sea urchin Strongylocentrotus droebachiensis

The phaeophyte alga Desmarestia munda (Order Desmarestiales) concentrates sulfuric acid (H₂SO₄) in cell vacuoles. The primary function of the accumulated acid in the alga's life history is unknown. To investigate if sulfuric acid works as a chemical defense, grazing by the sea urchin herbivore Strongylocentrotus droebachiensis on D. munda was compared to grazing on four common non-acidic phaeophyte algae (Alaria marginata, Laminaria saccharina, Costaria costata, and Nereocystis luetkeana) in multiple-choice feeding preference experiments. Both fed and starved urchins discriminated among algae; N. luetkeana and L. saccharina were consumed preferentially to D. munda and A. marginata. Fed urchins consumed a smaller quantity of A. marginata and D. munda than the starved urchins (15-28% less). The lack of preference for D. munda was not correlated with the presence of polyphenolic compounds or with nitrogen concentration. A. marginata had a high polyphenolic concentration (0.8% DM, dry mass) compared to that of the other four algae (0.08-0.24% DM). The nutritional content (C:N ratio and percent nitrogen) of the five algae ranged from 10.8 to 16.2 and from 2.08 to 3.55, respectively, but was not correlated with food choice by the urchins. Sulfuric acid made up nearly 16% DM of D. munda tissue and the pH of homogenized tissues averaged 1.99 pH units. Sulfuric acid, added to prepared food to provide a range of pH values from 1.9 to 6.2 pH units, deterred feeding by urchins at pH values Г.5 pH units. This result provides direct evidence that sulfuric acid at concentrations found in algal tissues deters grazing by S. droebachiensis. Therefore, acid accumulation in D. munda provides an important ecological defense against grazing by this herbivore.     

Modeling the population dynamics of cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) over a wide area in northern China

The cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) is one of the most serious crop pests in northern China, calling for accurate prediction of pest outbreaks and strategies for pest control. A computer model is developed to simulate the population dynamics of H. armigera over a wide area in northern China. The area considered covers 12 provinces where serious outbreaks of H. armigera have been observed. In this model, pest development is driven by local ambient temperature, and adults migrate long distances between regions and select preferred hosts for oviposition within a region. Six types of host including cotton, wheat, corn, peanut, soybean and a single category composed of all other minor hosts are considered in this model. Survival rates of eggs and larvae are based on life-table data, and simulated as a function of host type, host phenology and temperature. The incidence of diapause depends on temperature and photoperiod experienced during the larval stage. Survival rate of non-diapause pupae is a nonlinear function of rainfall, and overwinter survival rate is a nonlinear function of temperature. Insecticide is applied when population density exceeds the economic threshold on a host crop within a region. Comparisons of model output with light-trap data indicate that our model reflects the pest population dynamics over a wide area, and could potentially be used for testing novel pest control strategies in northern China.     

Farmers' perceptions of constraints to plantain production in Ghana

SUMMARY

Plantain (Musa AAB), a primary food crop in Ghana, is a key component in sustainable agricultural systems in high rainfall zones. Recently, there has been a substantial yield decline and reduction in plantation life. To elucidate the context in which intervention strategies should be developed, a Participatory Rural Appraisal (PRA) was conducted at five villages in the major plantain-producing belt of Ghana. The importance of plantain as a preferred food was confirmed, although farmers tended to sell plantain for cash income, using cheaper, less preferred alternatives for home consumption. Farmers identified decreasing soil fertility, the high cost of labour for weeding, pests and diseases, lack of good quality planting material and marketing-related issues as the major production constraints. Due to declining productivity in less fertile regions, plantain has been replaced with other food crops such as cassava and maize. Farmers overestimated the importance of insect pests but were unaware of the extensive damage that could be caused by nematodes and the foliar disease, black sigatoka. They observed, however, that pest damage is more severe when soil fertility is poor. Clearly, integrated pest management is likely to be most effective when practiced within the context of cropping systems management; sustainable strategies that are being developed for resource-limited plantain farmers in Ghana are discussed.     

Microbial activities in the emitted hydrothermal waters of the Galápagos rift vents

In-situ measurement of chemolithotrophic and some heterotrophic microbial activities were made in the immediate vicinity of actively discharging hydrothermal vents of the Galápagos Rift region at depths of 2 500 to 2 600 m. The CO₂-assimilation or chemosynthesis productivity in the emitted vent waters, freshly mixed with oxygenated ambient seawater of 2°C, was minor compared to the bacterial biomass produced within the subsurface vent system prior to emission. Uptake of acetate and glucose indicated the presence of mixotrophic or facultatively chemolithotrophic bacteria in the emitted vent waters in agreement with isolations. Demonstration of ribulose bisphosphate carboxylase and phosphoenol pyruvate carboxylase in cultures of thiobacilli isolated from these vent water supports the notion that chemoautotrophic sulfur-oxidizing bacteria are one of the sources of primary production in the form of particulate organic carbon for filtering organisms in the deep sea hydrothermal environment. The rates of bacterial metabolic activities in emitted vent water are too low for the amount of invertebrate biomass and the rate of its growth and maintenance. Therefore, the larger portion of chemosynthetic sustenance of deep sea vent ecosystems appears to be based on symbiotic associations between bacteria and invertebrates and on surface attached bacteria.     

Modelling the impact of defoliation and leaf damage on forest plantation function and production

After presenting a short review of process-based model requirements to capture the plant dynamic response to defoliation, this paper describes the development and testing of a model of crown damage and defoliation for Eucalyptus. A model that calculates light interception and photosynthetic production for canopies that vary spatially and temporally in leaf area and photosynthetic properties is linked to the forest growth model CABALA. The process of photosynthetic up-regulation following defoliation is modelled with a simple conditional switch that triggers up-regulation when foliar damage or removal causes the ratio of functional leaf area to living tissue in the tree to change.We show that the model predicts satisfactorily when validated with trees of Eucalyptus nitens and Eucalyptus globulus from a range of sites of different ages, subject to different types of stress and different types of defoliation events (R² = 0.96 across a range of sites). However, the complexity of particular situations can cause the model to fail (e.g. very heavy defoliation events where branch death occurs).It is concluded that while the model will not cope with all situations, an appropriate level of generality has been captured to represent many of the physiological processes and feedbacks that occur following defoliation or leaf damage. This makes the model useful for guiding management interventions following pest attack and allows the development of scenarios including climate change impact analyses and decision-making on the merits of post-defoliation fertilisation to expedite recovery.