Organic agriculture and nitrous oxide emissions at sub-zero soil temperatures

In the Red River Valley of the upper midwestern United States, soil temperatures often remain below freezing during winter and N2O emissions from frozen cropland soils is assumed to be negligible. This study was conducted to determine the strength of N2O emissions and denitrification when soil temperatures were below zero for a manure-amended, certified organic field (T2O) compared with an unamended, conventionally managed field (T2C). Before manure application, both fields were similar with respect to autotrophic and heterotrophic N2O production and N2O flux at the soil surface (0.15+/-0.05 mg N2O-N m-2 d-1 for T2O and 0.12+/-0.06 mg N2O-N m-2 d-1 for T2C). After application of pelletized, dehydrated manure, average daily flux (based on time-integrated fluxes from 20 November to 8 April), was 1.19+/-0.34 mg N2O-N m-2 d-1 for T2O and 0.47+/-0.37 mg N2O-N m-2 d-1 for T2C. Denitrification for intact cores measured in the laboratory at -2.5 degrees C was greater for organically managed soils, although only marginally significant (p<0.1). Cumulative emissions for all winter measurements (from 16 November to 8 April) averaged 1.63 kg N2O-N ha-1 for T2O and 0.64 kg N2O-N ha-1 for T2C. Biological N2O production was evident at sub-zero soil temperatures, with winter emissions exceeding those measured in late summer. Late autumn manure application enhanced cumulative N2O-N emissions by 0.9 kg ha-1.     

Greenhouse gas emissions and soil indicators four years after manure and compost applications

Understanding how carbon, nitrogen, and key soil attributes affect gas emissions from soil is crucial for alleviating their undesirable residual effects that can linger for years after termination of manure and compost applications. This study was conducted to evaluate the emission of soil CO2, N2O, and CH4 and soil C and N indicators four years after manure and compost application had stopped. Experimental plots were treated with annual synthetic N fertilizer (FRT), annual and biennial manure (MN1 and MN2, respectively), and compost (CP1 and CP2, respectively) from 1992 to 1995 based on removal of 151 kg N ha(-1) yr(-1) by continuous corn (Zea mays L.). The control (CTL) plots received no input. After 1995, only the FRT plots received N fertilizer in the spring of 1999. In 1999, the emissions of CO2 were similar between control and other treatments. The average annual carbon input in the CTL and FRT plots were similar to soil CO2-C emission (4.4 and 5.1 Mg C ha(-1) yr(-1), respectively). Manure and compost resulted in positive C and N balances in the soil four years after application. Fluxes of CH4-C and N2O-N were nearly zero, which indicated that the residual effects of manure and compost four years after application had no negative influence on soil C and N storage and global warming. Residual effects of compost and manure resulted in 20 to 40% higher soil microbial biomass C, 42 to 74% higher potentially mineralizable N, and 0.5 unit higher pH compared with the FRT treatment. Residual effects of manure and compost on CO2, N20, and CH4 emissions were minimal and their benefits on soil C and N indicators were more favorable than that of N fertilizer.     

Long-term trends in nitrous oxide emissions, soil nitrogen, and crop yields of till and no-till cropping systems

No-till cropping can increase soil C stocks and aggregation but patterns of long-term changes in N2O emissions, soil N availability, and crop yields still need to be resolved. We measured soil C accumulation, aggregation, soil water, N2O emissions, soil inorganic N, and crop yields in till and no-till corn-soybean-wheat rotations between 1989 and 2002 in southwestern Michigan and investigated whether tillage effects varied over time or by crop. Mean annual NO3- concentrations in no-till were significantly less than in conventional till in three of six corn years and during one year of wheat production. Yields were similar in each system for all 14 years but three, during which yields were higher in no-till, indicating that lower soil NO3- concentrations did not result in lower yields. Carbon accumulated in no-till soils at a rate of 26 g C m(-2) yr(-1) over 12 years at the 0- to 5-cm soil depth. Average nitrous oxide emissions were similar in till (3.27 +/- 0.52 g N ha d(-1)) and no-till (3.63 +/- 0.53 g N ha d(-1)) systems and were sufficient to offset 56 to 61% of the reduction in CO2 equivalents associated with no-till C sequestration. After controlling for rotation and environmental effects by normalizing treatment differences between till and no-till systems we found no significant trends in soil N, N2O emissions, or yields through time. In our sandy loam soils, no-till cropping enhances C storage, aggregation, and associated environmental processes with no significant ecological or yield tradeoffs.     

Nitrous oxide fluxes in turfgrass: effects of nitrogen fertilization rates and types

Urban ecosystems are rapidly expanding and their effects on atmospheric nitrous oxide (N2O) inventories are unknown. Our objectives were to: (i) measure the magnitude, seasonal patterns, and annual emissions of N2O in turfgrass; (ii) evaluate effects of fertilization with a high and low rate of urea N; and (iii) evaluate effects of urea and ammonium sulfate on N2O emissions in turfgrass. Nitrogen fertilizers were applied to turfgrass: (i) urea, high rate (UH; 250 kg N ha(-1) yr(-1)); (ii) urea, low rate (UL; 50 kg N ha(-1) yr(-1)); and (iii) ammonium sulfate, high rate (AS; 250 kg N ha(-1) y(-1)); high N rates were applied in five split applications. Soil fluxes of N2O were measured weekly for 1 yr using static surface chambers and analyzing N2O by gas chromatography. Fluxes of N2O ranged from -22 microg N2O-N m(-2) h(-1) during winter to 407 microg N2O-N m(-2) h(-1) after fall fertilization. Nitrogen fertilization increased N2O emissions by up to 15 times within 3 d, although the amount of increase differed after each fertilization. Increases were greater when significant precipitation occurred within 3 d after fertilization. Cumulative annual emissions of N2O-N were 1.65 kg ha(-1) in UH, 1.60 kg ha(-1) in AS, and 1.01 kg ha(-1) in UL. Thus, annual N2O emissions increased 63% in turfgrass fertilized at the high compared with the low rate of urea, but no significant effects were observed between the two fertilizer types. Results suggest that N fertilization rates may be managed to mitigate N2O emissions in turfgrass ecosystems.     

Nitrous oxide and ammonia fluxes in a soybean field irrigated with swine effluent

In the United States, swine (Sus scrofa) operations produce more than 14 Tg of manure each year. About 30% of this manure is stored in anaerobic lagoons before application to land. While land application of manure supplies nutrients for crop production, it may lead to gaseous emissions of ammonia (NH3) and nitrous oxide (N2O). Our objectives were to quantify gaseous fluxes of NH3 and N2O from effluent applications under field conditions. Three applications of swine effluent were applied to soybean [Glycine max (L.) Merr. 'Brim'] and gaseous fluxes were determined from gas concentration profiles and the flux-gradient gas transport technique. About 12% of ammonium (NH4-N) in the effluent was lost through drift or secondary volatilization of NH3 during irrigation. An additional 23% was volatilized within 48 h of application. Under conditions of low windspeed and with the wind blowing from the lagoon to the field, atmospheric concentrations of NH3 increased and the crop absorbed NH3 at the rate of 1.2 kg NH3 ha(-1) d(-1), which was 22 to 33% of the NH3 emitted from the lagoon during these periods. Nitrous oxide emissions were low before effluent applications (0.016 g N2O-N ha(-1) d(-1)) and increased to 25 to 38 g N2O-N ha(-1) d(-1) after irrigation. Total N2O emissions during the measurement period were 4.1 kg N2O-N ha(-1), which was about 1.5% of total N applied. The large losses of NH3 and N2O illustrate the difficulty of basing effluent irrigation schedules on N concentrations and that NH3 emissions can significantly contribute to N enrichment of the environment.     

Comparison of DAYCENT-simulated and measured nitrous oxide emissions from a corn field

Accurate assessment of N(2)O emission from soil requires continuous year-round and spatially extensive monitoring or the use of simulation that accurately and precisely predict N(2)O fluxes based on climatic, soil, and agricultural system input data. DAYCENT is an ecosystem model that simulates, among other processes, N(2)O emissions from soils. The purpose of the study was to compare N(2)O fluxes predicted by the DAYCENT model to measured N(2)O fluxes from an experimental corn field in central Iowa. Soil water content temperature and inorganic N, simulated by DAYCENT were compared to measured values of these variables. Field N(2)O emissions were measured using four replicated automated chambers at 6-h intervals, from day of year (DOY) 42 through DOY 254 of 2006. We observed that DAYCENT generally accurately predicted soil temperature, with the exception of winter when predicted temperatures tended to be lower than measured values. Volumetric water contents predicted by DAYCENT were generally lower than measured values during most of the experimental period. Daily N(2)O emissions simulated by DAYCENT were significantly correlated to field measured fluxes; however, time series analyses indicate that the simulated fluxes were out of phase with the measured fluxes. Cumulative N(2)O emission calculated from the simulations (3.29 kg N(2)O-N ha(-1)) was in range of the measured cumulative N(2)O emission (4.26 +/- 1.09 kg N(2)O-N ha(-1)).     

Greenhouse gas emissions from two soils receiving nitrogen fertilizer and swine manure slurry

The interactive effects of soil texture and type of N fertility (i.e., manure vs. commercial N fertilizer) on N(2)O and CH(4) emissions have not been well established. This study was conducted to assess the impact of soil type and N fertility on greenhouse gas fluxes (N(2)O, CH(4), and CO(2)) from the soil surface. The soils used were a sandy loam (789 g kg(-1) sand and 138 g kg(-1) clay) and a clay soil (216 g kg(-1) sand, and 415 g kg(-1) clay). Chamber experiments were conducted using plastic buckets as the experimental units. The treatments applied to each soil type were: (i) control (no added N), (ii) urea-ammonium nitrate (UAN), and (iii) liquid swine manure slurry. Greenhouse gas fluxes were measured over 8 weeks. Within the UAN and swine manure treatments both N(2)O and CH(4) emissions were greater in the sandy loam than in the clay soil. In the sandy loam soil N(2)O emissions were significantly different among all N treatments, but in the clay soil only the manure treatment had significantly higher N(2)O emissions. It is thought that the major differences between the two soils controlling both N(2)O and CH(4) emissions were cation exchange capacity (CEC) and percent water-filled pore space (%WFPS). We speculate that the higher CEC in the clay soil reduced N availability through increased adsorption of NH(4)(+) compared to the sandy loam soil. In addition the higher average %WFPS in the sandy loam may have favored higher denitrification and CH(4) production than in the clay soil.     

Emissions of ammonia, methane, carbon dioxide, and nitrous oxide from dairy cattle housing and manure management systems

Concentrated animal feeding operations emit trace gases such as ammonia (NH?), methane (CH?), carbon dioxide (CO?), and nitrous oxide (N?O). The implementation of air quality regulations in livestock-producing states increases the need for accurate on-farm determination of emission rates. The objective of this study was to determine the emission rates of NH?, CH?, CO?, and N?O from three source areas (open lots, wastewater pond, compost) on a commercial dairy located in southern Idaho. Gas concentrations and wind statistics were measured each month and used with an inverse dispersion model to calculate emission rates. Average emissions per cow per day from the open lots were 0.13 kg NH?, 0.49 kg CH?, 28.1 kg CO?, and 0.01 kg N?O. Average emissions from the wastewater pond (g m(-2) d(-1)) were 2.0 g NH?, 103 g CH?, 637 g CO?, and 0.49 g N?O. Average emissions from the compost facility (g m(-2) d(-1)) were 1.6 g NH?, 13.5 g CH?, 516 g CO?, and 0.90 g N?O. The combined emissions of NH?, CH?, CO?, and N?O from the lots, wastewater pond and compost averaged 0.15, 1.4, 30.0, and 0.02 kg cow(-1) d(-1), respectively. The open lot areas generated the greatest emissions of NH?, CO?, and N?O, contributing 78, 80, and 57%, respectively, to total farm emissions. Methane emissions were greatest from the lots in the spring (74% of total), after which the wastewater pond became the largest source of emissions (55% of total) for the remainder of the year. Data from this study can be used to develop trace gas emissions factors from open-lot dairies in southern Idaho and potentially other open-lot production systems in similar climatic regions.     

Nitrogen source effects on nitrous oxide emissions from irrigated no-till corn

Nitrogen fertilization is essential for optimizing crop yields; however, it may potentially increase nitrous oxide (N2O) emissions. The study objective was to assess the ability of commercially available enhanced-efficiency N fertilizers to reduce N2O emissions following their application in comparison with conventional dry granular urea and liquid urea-ammonium nitrate (UAN) fertilizers in an irrigated no-till (NT) corn (Zea mays L.) production system. Four enhanced-efficiency fertilizers were evaluated: two polymer-coated urea products (ESN and Duration III) and two fertilizers containing nitrification and urease inhibitors (SuperU and UAN+AgrotainPlus). Nitrous oxide fluxes were measured during two growing seasons using static, vented chambers and a gas chromatograph analyzer. Enhanced-efficiency fertilizers significantly reduced growing-season N2O-N emissions in comparison with urea, including UAN. SuperU and UAN+AgrotainPlus had significantly lower N2O-N emissions than UAN. Compared with urea, SuperU reduced N2O-N emissions 48%, ESN 34%, Duration III 31%, UAN 27%, and UAN+AgrotainPlus 53% averaged over 2 yr. Compared with UAN, UAN+AgrotainPlus reduced N2O emissions 35% and SuperU 29% averaged over 2 yr. The N2O-N loss as a percentage of N applied was 0.3% for urea, with all other N sources having significantly lower losses. Grain production was not reduced by the use of alternative N sources. This work shows that enhanced-efficiency N fertilizers can potentially reduce N2O-N emissions without affecting yields from irrigated NT corn systems in the semiarid central Great Plains.     

Dairy farm effluent effects on urine patch nitrous oxide and carbon dioxide emissions

Dairy farm effluent (DFE) comprises animal feces, urine, and wash-down water collected at the milking shed. This is collected daily during the milking season and sprayed onto grazed dairy pastures. Urine patches in grazed pastures make a significant contribution to anthropogenic N(2)O emissions. The DFE could potentially mitigate N(2)O emissions by influencing the N(2)O to dinitrogen (N(2)) ratio, since it contains water-soluble carbon (WSC). Alternatively, DFE may enhance N(2)O emissions from urine patches. The application of DFE may also provide a substrate for the production of CO(2) in pasture soils. The effects of DFE on the CO(2) and N(2)O emissions from urine patches are unknown. Thus a laboratory experiment was performed where repeated DFE applications were made to repacked soil cores. Dairy farm effluent was applied at 0, 7, or 14 d after urine deposition. The urine was applied once on Day 0. Urine contained (15)N-enriched urea. Measurements of N(2)O, N(2), and carbon dioxide (CO(2)) fluxes, soil pH, and soil inorganic N concentrations were made. After 43 d the DFE had not mitigated N(2)O fluxes from urine patches. A small increase in the N(2)O flux occurred from the urine-treated soils where DFE was applied 1 wk after urine deposition. The amount of WSC applied in the DFE proved to be insignificant compared with the amount of soil C released as CO(2) following urine application. The priming of soil C in urine patches has implications for the understanding of soil C processes in grazed pasture ecosystems and the budgeting of C within these ecosystems.     

Tillage and field scale controls on greenhouse gas emissions

There is a lack of understanding of how associations among soil properties and management-induced changes control the variability of greenhouse gas (GHG) emissions from soil. We performed a laboratory investigation to quantify relationships between GHG emissions and soil indicators in an irrigated agricultural field under standard tillage (ST) and a field recently converted (2 yr) to no-tillage (NT). Soil cores (15-cm depth) were incubated at 25 degrees C at field moisture content and 75% water holding capacity. Principal component analysis (PCA) identified that most of the variation of the measured soil properties was related to differences in soil C and N and soil water conditions under ST, but soil texture and bulk density under NT. This trend became more apparent after irrigation. However, principal component regression (PCR) suggested that soil physical properties or total C and N were less important in controlling GHG emissions across tillage systems. The CO2 flux was more strongly determined by microbial biomass under ST and inorganic N content under NT than soil physical properties. Similarly, N2O and CH4 fluxes were predominantly controlled by NO3- content and labile C and N availability in both ST and NT soils at field moisture content, and NH4+ content after irrigation. Our study indicates that the field-scale variability of GHG emissions is controlled primarily by biochemical parameters rather than physical parameters. Differences in the availability and type of C and N sources for microbial activity as affected by tillage and irrigation develop different levels and combinations of field-scale controls on GHG emissions.     

Emission of the greenhouse gases nitrous oxide and methane from constructed wetlands in europe

The potential atmospheric impact of constructed wetlands (CWs) should be examined as there is a worldwide increase in the development of these systems. Fluxes of N(2)O, CH(4), and CO(2) have been measured from CWs in Estonia, Finland, Norway, and Poland during winter and summer in horizontal and vertical subsurface flow (HSSF and VSSF), free surface water (FSW), and overland and groundwater flow (OGF) wetlands. The fluxes of N(2)O-N, CH(4)-C, and CO(2)-C ranged from -2.1 to 1000, -32 to 38 000, and -840 to 93 000 mg m(-2) d(-1), respectively. Emissions of N(2)O and CH(4) were significantly higher during summer than during winter. The VSSF wetlands had the highest fluxes of N(2)O during both summer and winter. Methane emissions were highest from the FSW wetlands during wintertime. In the HSSF wetlands, the emissions of N(2)O and CH(4) were in general highest in the inlet section. The vegetated ponds in the FSW wetlands released more N(2)O than the nonvegetated ponds. The global warming potential (GWP), summarizing the mean N(2)O and CH(4) emissions, ranged from 5700 to 26000 and 830 to 5100 mg CO(2) equivalents m(-2) d(-1) for the four CW types in summer and winter, respectively. The wintertime GWP was 8.5 to 89.5% of the corresponding summertime GWP, which highlights the importance of the cold season in the annual greenhouse gas release from north temperate and boreal CWs. However, due to their generally small area North European CWs were suggested to represent only a minor source for atmospheric N(2)O and CH(4).     

Net global warming potential and greenhouse gas intensity in irrigated cropping systems in northeastern Colorado

The impact of management on global warming potential (GWP), crop production, and greenhouse gas intensity (GHGI) in irrigated agriculture is not well documented. A no-till (NT) cropping systems study initiated in 1999 to evaluate soil organic carbon (SOC) sequestration potential in irrigated agriculture was used in this study to make trace gas flux measurements for 3 yr to facilitate a complete greenhouse gas accounting of GWP and GHGI. Fluxes of CO2, CH4, and N2O were measured using static, vented chambers, one to three times per week, year round, from April 2002 through October 2004 within conventional-till continuous corn (CT-CC) and NT continuous corn (NT-CC) plots and in NT corn-soybean rotation (NT-CB) plots. Nitrogen fertilizer rates ranged from 0 to 224 kg N ha(-1). Methane fluxes were small and did not differ between tillage systems. Nitrous oxide fluxes increased linearly with increasing N fertilizer rate each year, but emission rates varied with years. Carbon dioxide efflux was higher in CT compared to NT in 2002 but was not different by tillage in 2003 or 2004. Based on soil respiration and residue C inputs, NT soils were net sinks of GWP when adequate fertilizer was added to maintain crop production. The CT soils were smaller net sinks for GWP than NT soils. The determinant for the net GWP relationship was a balance between soil respiration and N2O emissions. Based on soil C sequestration, only NT soils were net sinks for GWP. Both estimates of GWP and GHGI indicate that when appropriate crop production levels are achieved, net CO2 emissions are reduced. The results suggest that economic viability and environmental conservation can be achieved by minimizing tillage and utilizing appropriate levels of fertilizer.     

Nitrous oxide dynamics in a braided river system, New Zealand

Recently the Intergovernmental Panel on Climate Change (IPCC) emission factor EF5-r was revised downward to a value of 0.0025 kg N?O-N per kg NO?-N leached. It was not reduced further due to the continued uncertainty surrounding the dynamics of N?O in river systems. There have been few studies where river system N?O yields and fluxes have been measured. In this study, we examined the relationship between NO?-N and N?O-N fluxes at 10 sites along a braided river system (84 km) over a 397-d period. Isotopic analysis of NO?-N river water samples and the potential agricultural nitrogen (N) sources demonstrated that the NO?-N came from agricultural or sewage sources. Percent saturation of N?O varied with site and date (average, 114%) and correlated with river N?O-N concentrations. Modeled N?O fluxes (16-30 μg m(-2) h(-1)) from five sites were strongly related to river NO?-N concentrations ( r2 = 0.86). The modeled N?O-N fluxes ranged from 39 to 81% of the IPCC-derived emissions based on the NO?-N load in the river over 397 d and do not support further lowering of the EF5-r. Further in situ river studies are required to verify the N?O-N fluxes and the calculated gas transfer velocity values for these braided river systems.     

Cattle feedlot soil moisture and manure content: I. Impacts on greenhouse gases, odor compounds, nitrogen losses, and dust

Beef cattle feedlots face serious environmental challenges associated with manure management, including greenhouse gas, odor, NH3, and dust emissions. Conditions affecting emissions are poorly characterized, but likely relate to the variability of feedlot surface moisture and manure contents, which affect microbial processes. Odor compounds, greenhouse gases, nitrogen losses, and dust potential were monitored at six moisture contents (0.11, 0.25, 0.43, 0.67, 1.00, and 1.50 g H2O g(-1) dry matter [DM]) in three artificial feedlot soil mixtures containing 50, 250, and 750 g manure kg(-1) total (manure + soil) DM over a two-week period. Moisture addition produced three microbial metabolisms: inactive, aerobic, and fermentative at low, moderate, and high moisture, respectively. Manure content acted to modulate the effect of moisture and enhanced some microbial processes. Greenhouse gas (CO2, N2O, and CH4) emissions were dynamic at moderate to high moisture. Malodorous volatile fatty acid (VFA) compounds did not accumulate in any treatments, but their persistence and volatility varied depending on pH and aerobic metabolism. Starch was the dominant substrate fueling both aerobic and fermentative metabolism. Nitrogen losses were observed in all metabolically active treatments; however, there was evidence for limited microbial nitrogen uptake. Finally, potential dust production was observed below defined moisture thresholds, which were related to manure content of the soil. Managing feedlot surface moisture within a narrow moisture range (0.2-0.4 g H2O g(-1) DM) and minimizing the accumulation of manure produced the optimum conditions that minimized the environmental impact from cattle feedlot production.     

Nitrous oxide emissions from an ultisol of the humid tropics under maize-groundnut rotation

Nitrous oxide (N20) contributes to global climate change and agricultural soils seem to be the major source. Lack of information led to this study on the influence of different amounts and sources of nitrogen on N2O emission from a maize (Zea mays L.)-groundnut (Arachis hypogae L.) crop rotation in an Ultisol of the humid tropics. The treatments were: inorganic N + crop residues (NC), inorganic N only (RN), and half of inorganic N + crop residues + chicken manure (NCM). The corresponding amount of N applied was 322, 180, and 400 kg ha(-1) yr(-1), respectively. The N2O emissions depended on the amounts and types of N. A maximum peak (9,889 +/- 2,106 microg N2O-N m(-2) d(-1)) was detected at 2 wk before maize sowing amended with chicken manure, showing a persistent influence on N transformations and N2O release. The mineral N from either applied source became low by 2 to 4 wk, coinciding with the small N2O fluxes or its consumption to a few isolated instances. The N2O flux significantly correlated with the mineral N and water-filled pore spaces. The direct annual N2O emission was 3.94 +/- 0.23, 1.90 +/- 0.08, and 1.41 +/- 0.07 kg N2O-N ha(-1) from the NCM, NC, and RN treatments, respectively. The corresponding N2O-N loss of the applied N plus N fixed by groundnut was 0.83, 0.49, and 0.59%. Overestimations of direct annual N2O emission using the Intergovernmental Panel on Climate Change (IPCC) methodology suggest a location-specific emission factor for variable N sources to be considered.     

Effect of sampling frequency on estimates of cumulative nitrous oxide emissions

It is generally recognized that soil N(2)O emissions can exhibit pronounced day-to-day variations; however, measurements of soil N(2)O flux with soil chambers typically are done only at discrete points in time. This study evaluated the impact of sampling frequency on the precision of cumulative N(2)O flux estimates calculated from field measurements. Automated chambers were deployed in a corn/soybean field and used to measure soil N(2)O fluxes every 6 h from 25 Feb. 2006 through 11 Oct. 2006. The chambers were located in two positions relative to the fertilizer bands-directly over a band or between fertilizer bands. Sampling frequency effects on cumulative N(2)O-N flux estimation were assessed using a jackknife technique where populations of N(2)O fluxes were constructed from the average daily fluxes measured in each chamber. These test populations were generated by selecting measured flux values at regular time intervals ranging from 1 to 21 d. It was observed that as sampling interval increased from 7 to 21 d, variances associated with cumulative flux estimates increased. At relatively frequent sampling intensities (i.e., once every 3 d) N(2)O-N flux estimates were within +/-10% of the expected value at both sampling positions. As the time interval between sampling was increased, the deviation in estimated cumulative N(2)O flux increased, such that sampling once every 21 d yielded estimates within +60% and -40% of the actual cumulative N(2)O flux. The variance of potential fluxes associated with the between-band positions was less than the over-band position, indicating that the underlying temporal variability impacts the efficacy of a given sampling protocol.     

Gaseous nitrogen emissions and mineral nitrogen transformations as affected by reclaimed effluent application

Irrigation with reclaimed effluent (RE) is essential in arid and semiarid regions. Reclaimed effluent has the potential to stimulate gaseous N losses and affect other soil N processes. No direct measurements of the N2 and N2O emissions from Mediterranean soils have been conducted so far. We used the 15N gas flux method in a field and a laboratory experiment to study the effect of RE irrigation on gaseous N losses and other N transformations in a Grumosol (Chromoxerert) soil. The fluxes of N2, N2O, and NH3 were measured from six Grumosol lysimeters following application of either fresh water or RE. The N fertilizer was applied either as 15NH4 or 15NO3. Only up to 0.3% from the applied N fertilizer was lost as N2O + NH3. Reclaimed effluent enhanced the losses of NH3, but did not affect those of N2O. Nitrification and denitrification were equally important to N2O production. Laboratory incubations were performed to both confirm the influence of the irrigation water type and to test the effect of moisture content. Significant quantities of N2 and N2O (up to 3.1% of the applied fertilizer) were emitted from saturated soils. Reclaimed effluent application did not induce higher N2O emissions, yet significantly more (approximately 33%) N2 was emitted from RE-irrigated soils. Denitrification contributed up to 75% of the N2O amounts emitted from saturated soils. Reclaimed effluent application inhibited nitrification in the Grumosol by 15 to 25% and induced NO2 accumulation in soils incubated at a field-capacity moisture content.     

Nitrous oxide accumulation in soils from riparian buffers of a coastal plain watershed carbon/nitrogen ratio control

Riparian buffers are used throughout the world for the protection of water bodies from nonpoint-source nitrogen pollution. Few studies of riparian or treatment wetland denitrification consider the production of nitrous oxide (N2O). The objectives of this research were to ascertain the level of potential N2O production in riparian buffers and identify controlling factors for N2O accumulations within riparian soils of an agricultural watershed in the southeastern Coastal Plain of the USA. Soil samples were obtained from ten sites (site types) with different agronomic management and landscape position. Denitrification enzyme activity (DEA) was measured by the acetylene inhibition method. Nitrous oxide accumulations were measured after incubation with and without acetylene (baseline N2O production). The mean DEA (with acetylene) was 59 microg N2O-N kg(-1) soil h(-1) for all soil samples from the watershed. If no acetylene was added to block conversion of N2O to N2, only 15 microg N2O-N kg(-1) soil h(-1) were accumulated. Half of the samples accumulated no N2O. The highest level of denitrification was found in the soil surface layers and in buffers impacted by either livestock waste or nitrogen from legume production. Nitrous oxide accumulations (with acetylene inhibition) were correlated to soil nitrogen (r2=0.59). Without acetylene inhibition, correlations with soil and site characteristics were lower. Nitrous oxide accumulations were found to be essentially zero, if the soil C/N ratios>25. Soil C/N ratios may be an easily measured and widely applicable parameter for identification of potential hot spots of N2O productions from riparian buffers.     

Real-time, high-resolution quantitative measurement of multiple soil gas emissions: selected ion flow tube mass spectrometry

A new technique is presented for the rapid, high-resolution identification and quantification of multiple trace gases above soils, at concentrations down to 0.01 microL L(-1) (10 ppb). The technique, selected ion flow tube mass spectrometry (SIFT-MS), utilizes chemical ionization reagent ions that react with trace gases but not with the major air components (N2, O2, Ar, CO2). This allows the real-time measurement of multiple trace gases without the need for preconcentration, trapping, or chromatographic separation. The technique is demonstrated by monitoring the emission of ammonia and nitric oxide, and the search for volatile organics, above containerized soil samples treated with synthetic cattle urine. In this model system, NH3 emissions peaked after 24 h at 2000 nmol m(-2) s(-1) and integrated to approximately 7% of the urea N applied, while NO emissions peaked about 25 d after urine addition at approximately 140 nmol m(-2) s(-1) and integrated to approximately 10% of the applied urea N. The monitoring of organics along with NH3 and NO was demonstrated in soils treated with synthetic urine, pyridine, and dimethylamine. No emission of volatile nitrogen organics from the urine treatments was observed at levels >0.01% of the applied nitrogen. The SIFT method allows the simultaneous in situ measurement of multiple gas components with a high spatial resolution of < 10 cm and time resolution <20 s. These capabilities allow, for example, identification of emission hotspots, and measurement of localized and rapid variations above agricultural and contaminated soils, as well as integrated emissions over longer periods.