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In an unprecedented short time, the polymerase chain reaction (PCR) has developed into an indispensible analytical tool which facilitates gene analyses and recombinant techniques, enables DNA detection from single cells, and permits sequence determinations even from extinct species. Like the PCR process itself, the number of laboratories making use of this novel technique has been growing exponentially. This report covers critically the PCR from the basic principle to selected examples. It illustrates the strengths of the method: speed, sensitivity, and versatility and discusses aspects which still need improvement and development.  相似文献   

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Die Interferone     
Interferons are a family of proteins synthesized by the cells of higher organisms as a first reaction to viral infections, preceding the immune response. They are also involved in the regulation of the immune system and therefore included among the lymphokines. Since they had been shown to have antitumor activity in vitro and in experimental animals, they were suggested to be powerful drugs in cancer therapy.  相似文献   

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Die Pterosaurier     
During the Mesozoic the pterosaurs, or flying reptiles, were widely spread over the world. The distribution of the pterosaur fossils shows a restriction to a zone within 40 ° latitude in relation to the paleoequators. Probably all pterosaurs had hairs, thus indicating homoiothermic conditions. The brain structure is similar to the bird's brain but relatively smaller. With regard to certain osteologic features the pterosaurs have to be classified as archosaurs. Their origin is not known and they probably became extinct due to the change of climate during the late Cretaceous.  相似文献   

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模具参数对模具寿命的影响   总被引:1,自引:0,他引:1  
运用正交试验设计方法对影响模具寿命的锻造温度、冲孔连皮厚度、飞边槽高度等三种因素进行了比较试验 ,找出了影响模具寿命的三种因素的主次顺序。  相似文献   

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Die Neuen Sterne     
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The Science of Nature -  相似文献   

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